Original Article
Korean J Parasitol. 1978 Dec;16(2):69-81. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1978.16.2.69
Copyright © 1978 by The Korean Society for Parasitology
Effects of prednisolone injection on the liver of the mouse inoculated with the adult worms of Clonorchis sinensis intraperitoneally
Soon-Hyung Lee,Chul-Yong Song and Je G. Chi
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Department of Pathology, College of Medicine, Seoul National University, Korea.
Abstract

In order to understand the effect of prednisolone injection on the histopathological changes of the mouse liver and the chronological changes of the worm structure of Clonorchis sinensis, when this fluke was inoculated to the mouse intraperitoneally.

The recovery rate, survival rate, location and size of the inoculated worms as well as the histopathological changes of the liver were investigated for the comparison among the groups of mice, which were classified by number of worms and the duration of observation period.

The result obtained were summarized as follows:

1. The recovery rate and survival rate of the worms decreased especially 28 days after the inoculation.

2. Most of worms (45.5%) were collected from the peritoneal cavity, and some of worms were found tightly adherent to the capsules of the liver, spleen, intestine and diaphragm.

3. The mean worm size after inoculation was constantly smaller than that before inoculation.

4. Remarkable atrophy in the reproductive organs of the worm, such as spermatheca, testes, vitelline gland and ovary was frequently observed at the 10th day of inoculation.

5. Histopathologically the liver failed to show any parasitic worm inside the intrahepatic biliary system. However, multiple well formed egg-containing granulomas were present along the liver capsule. These necrotic granulomas were occasionally found under the fibrotic liver capsule. Focal necrosis and focal phlebitis together with vascular dilatation were prominent features of the liver.

6. The worms recovered in the capsule of the liver were degenerated and necrotized. Usually, there were remarkable capsulitis and granuloma formation around the eggs.

Figures


Figs. 1-4
Fig. 1. A normal worm (C. sinensis) of control group.

Fig. 2. A recovered worm from the abdominal cavity of the mouse, 4 days after the intraperitoneal inoculation. The spermatheca began to atrophy.

Fig. 3. Atrophy of the spermatheca, testes, ovary, vitellaria and scantiness of egg in the uterus were observed in the worm, 10 days after the inoculation.

Fig. 4. Remarkable atrophy of reproductive organs was visible in this worm, 28 days after the inoculation.



Figs. 5-8
Fig. 5. Hepatic lobule showing irregularity of nuclear size. H&E×400.

Fig. 6. Dilated venous channel is seen in the center, the wall of which is infiltrated with various inflammatory cells. H&E×400.

Fig. 7. A granuloma possibly parasitic origin is observed under the liver capsule. H&E×100.

Fig. 8. Photomicrograph of a necrotic granuloma(presumed to be a dead worm) excavation th liver capsule. H&E×100.


Tables


Table 1
Experimental design


Table 2
Number of worms recovered


Table 3
Number of worms survived


Table 4
Worms recovered from various parts of the body by group


Table 5
Worms recovered form various parts of the body by duration


Table 6
Worm size after inoculation by group


Table 7
Worm size recovered after inoculation by duration


Table 8
Atrophic changes of reproductive organs of the worms found by group


Table 9
Atrophic changes of reproductive organs of the worms found by duration


Table 10
Histopathological changes of mouse liver

References
1. Chan PH, Teoh TB. The pathology of Clonorchis sinensis infestation of the pancreas. J Pathol Bacteriol 1967;93(1):185–189.
  
2. Chung CS, et al. An epidemiological study of primary liver carcinoma in Busan area with special reference to clonorchiasis. Korean J Path 1976;10(1):33–46.
3. Chung KN. J Busan Med Coll 1975;15(1):217–227.
4. Faust EC, et al. Am J Hyg Monogr Ser 1927;8:1–207.
5. Hahn SS. Korean J Int Med 1961;4(6):417–444.
6. Hoeppli R. Chinese Med J 1933;47:1125–1141.
7. Hou PC. The pathology of Clonorchis sinensis infestation of the liver. J Pathol Bacteriol 1955;70(1):53–64.
  
8. Hou PC. The relationship between primary carcinoma of the liver and infestation with Clonorchis sinensis. J Pathol Bacteriol 1956;72(1):239–246.
  
9. Hou PC. Primary Carcinoma of Bile Duct of the Liver of the Cat (Felis Catus) Infested with Clonorchis Sinensis. J Pathol Bacteriol 1964;87:239–244.
  
10. Chang HP. Pathological Changes in the Intrahepatic Bile Ducts of Cats (Felis Catus) Infested with Clonorchis Sinensis. J Pathol Bacteriol 1965;89:357–364.
  
11. Kang TW. Seoul Univ J 1960;14:49–79.
12. Kim DH. Korean J Int Med 1966;9(1):33–48.
13. Kim JH. Korean J Int Med 1969;12(12):897–910.
14. Kim YI, et al. J Korean Surg Soc 1968;10(4):273–281.
15. Kim YI, et al. Seoul J Med 1974;15(3):247–253.
16. Kobayashi H. Saikingaku Zasshi 1912;202:1–66.
17. Komiya Y, et al. Jap J Med Sci Biol 1953;6(6):571–575.
 
18. Lee CS. Theses Catholic Med Coll 1967;12:103–129.
19. Mukoyama T. J Jap Path Soc 1921;11:443–446.
20. Rhee CH, Seo BS. [The Host-Parasite Relations Of Clonorchis Sinensis In The Mouse: Studies On The Development Of C. Sinensis In Mouse]. Korean J Parasitol 1968;6(3):101–109.
 
21. Seo BS. New Med J 1959;2(12):1389–1397.
22. Seo BS, et al. Seoul Univ Coll Theseon 1957;6:201–209.
23. Song CY, Lee SH, Chi JG. [An Experimental Study On The Mouse Intraperitoneal Inoculation Of The Adult Worm Of Clonorchis Sinensis]. Korean J Parasitol 1977;15(2):75–92.
 
24. Sun T, Chou ST, Gibson JB. Route of entry of Clonorchis sinensis to the mammalian liver. Exp Parasitol 1968;22(3):346–351.
  
25. Sun T, Gibson JB. Antigens of Clonorchis sinensis in experimental and human infections. Am J trop Med Hyg 1969;18(2):241–252.
 
26. Yoshimura H. Jap J Parasit 1965;14(5):445–451.
27. Yoshimura H. Jap J Parasit 1972;211:18–19.
28. Yoshimura H, et al. Jap J Parasit 1972;21(3):127–134.
29. Wykoff DE. Studies on Clonorchis sinensis III The host-parasite relations in the rabbit and observations on the relative susceptibility of certain laboratory hosts. J Parasitol 1958;44(5):461–466.
  
30. Wykoff DE, Lepes TJ. Studies on Clonorchis sinensis I Observation on the route of migration in the definitive host. Am J trop Med Hyg 1957;6(6):1061–1065.