Experimental studies on the efficacy of thiabendazole against the migratory stages of ascarids in mouse
Moo-Joon Cho
Department of Parasitology, Yonsei University Medical College, Korea.
Abstract
The following results were obtained in the present study concerning the effectiveness of thiabendazole upon the larvae of the migrating stages.
1) In the early observation group: The average number of larvae of the group treated with single dose and the group treated with three doses were reduced in proportion of 52.2%, 58.5 % respectively compared with control group.
2) In the late observation group: The reduction rate in the group treated with singel dose and group treated with three doses were 59.3 % and 63.2 % respectively compared with control group.
3) The reduction rates of larvae from liver and lungs in the early drug administration group were 72.9 % and 59.7 % respectively, and 14.9 % and 54.8 % in the delayed drug administration group.
4) In the group of drug given before and after infection, the number of recovered larvae were reduced 42.2 % and 31.1 % respectively compared with the control group.
5) The peak number in organs was delayed 1 to 2 days in the treated group than that of control group.
6) The survival period of the infected mouse was prolonged by the drug administration.
7) The pathological changes were reduced by the administration of the drug. Through above results, it was concluded that thiabendazole reduced the number of migrating larvae and delayed the normal migration of the larvae in tissues and reduced the pathological changes in the tissues.
Fig. 2 Comparison of number of Toxocara canis larvae recovered from each organs of mice infected and prescribed with thiabendazole 4 days post-infection.
Fig. 3 Comparison of number of Toxocara canis larvae recovered from each organs of mice infected and prescribed with thiabendazole 14 days post-infection.
Fig. 4 Comparison of number of Ascaris lumbricoides larvae recovered from each organs of mice infected and prescribed with thiabendazole 2 and 6 days post-infection.
Fig. 5 Comparison of number of Ascaris lumbricoides larvae recovered from each organs of mice infected and prescribed with thiabendazole pre-infection, from 24 hours post-infection.
Fig. 6 Change of number of Ascaris lumbricoides larvae recovered from mice infected and prescribed with thiabendazole pre-infection, from 24 hours post-infection.
Fig. 7 Change of number of Ascaris lumbricoides larvae recovered from mice liver infected and prescribed with thiabendazole pre-infection, from 24 hours post-infection.
Fig. 8 Change of number of Ascaris lumbricoides larvae recovered from mice lung infected and prescribed with thiabendazole pre-infection, from 24 hours post-infection.
Fig. 9 Change of number of Ascaris lumbricoides larvae recovered from mice intestine infected and prescribed with thiabendazole pre-infection, from 24 hours post-infection.
Fig. 10 Change of number of Ascaris lumbricoides larvae recovered from mice infected and prescribed with thiabendazole pre- and post-infection, from 24 hours post-infection.
Fig. 11 Survival rate of the mouse group infected with Ascaris lumbricoides and virus, and infected group prescribed with thiabendazole.
Appendix Fig. 3 Mouse lung tissue infected with Ascaris lumbricoides and virus 7 days post-infection
Appendix Fig. 4 Mouse lung tissue infected with Ascaris lumbricoides
Appendix Fig. 5 Mouse lung tissue prescribed with thiabendazole 24 hours pre-infection with Ascaris lumbricoides and infected with virus 7 days post-infection with Ascaris lumbricoides.
Appendix Fig. 6 Mouse lung tissue prescribed with thiabendazole 24 hours post-infection with Ascaris lumbricoides and infected with virus 7 days post-infection. with Ascaris lumbricoides.
Tables
Table 1 Number of Toxocara canis larvae recovered from each organs of mice infected and prescribed with thiabendazole 4 days post-infection
Table 2 Number of Toxocara canis larvae recovered from each organs of mice infected and prescribed with thiabendazole 14 days post-infection
Table 3 Number of Ascaris lumbricoides larvae recovered from each organs of mice infected and prescribed with thiabendazole 2 and 6 days post-infection
Table 4 Number of Ascaris lumbricoides larvae recovered from each organs of mice infected and prescribed with thiabendazole pre-infection, 24 hours post-infection
*Number of egg: 800
Table 5 Mortality rate of mice group infected with Ascaris lumbricoides and virus and group infected and treated with thiabendazole
I: Group infected with virus only, II: Group infected with Ascaris lumbricoides only III: Group infected with MAscaris lumbricoides and virus, IV: Group treated before infection and infected with Ascaris lumbricoides and virus, V: Group infected with Ascaris lumbricoides, treated and infected with virus.
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