INTRODUCTION
Heterophyes nocens and
Pygidiopsis summa are minute intestinal trematodes of the family Heterophyidae Odhner, 1914 (= heterophyids) infecting avian and mammalian hosts. Human infections with these heterophyids in the Republic of Korea were first reported from inhabitants residing in Okku-gun, Jeollabuk-do who habitually consumed raw flesh of mullets (
Seo et al., 1981a). Subsequently, their endemicities were recorded in western coastal areas, including Shinan-gun, Muan-gun, and Buan-gun (
Chai et al., 1997,
1998,
2000;
Chai and Lee, 2002). In many coastal islands, wide distribution of human
H. nocens and
P. summa infections was also reported (
Chai et al., 2004).
The main source of human infection with heterophyids, i.e.,
H. nocens and
P. summa, is brackish water fish, including mullets
Mugil cephalus and gobies
Acanthogobius flavimanus (
Chun, 1963;
Seo et al., 1980,
1981b). More than 20 years ago, high prevalence of these heterophyid metacercariae was reported in mullets and gobies collected from several southwestern coastal areas of the Republic of Korea (
Seo et al., 1981b). However, further information on geographical distribution of fish intermediate hosts, and prevalences and metacercarial densities of
H. nocens and
P. summa in fish, has not been available.
Metacercarial infections in fish could affect the prevalence of definitive hosts in local areas (
Seo et al., 1982;
Song et al., 1985;
Chai and Lee, 2002). Therefore, studies on metacercarial infections in brackish water fish could give useful information on the present status as well as strategies for prevention of human infections. The present study was performed to determine the current status of geographical distribution of
H. nocens and
P. summa infections based on detection of metacercariae in mullets and gobies in western, southern, and eastern coastal areas of the Republic of Korea.
DISCUSSION
In the present study, wide variations in the prevalence of H. nocens and P. summa metacercariae were observed in mullets and gobies according to geographical locality, where the fish were caught. Mullets from western coastal areas were more highly infected with metacercariae of H. nocens and P. summa than mullets from southern and eastern coastal areas. Metacercarial densities in mullets also varied according to locality, i.e., low in Ganghwa-gun (4 per fish) and high in Shinan-gun (271) for H. nocens, and low in Gangjin-gun (2) and high in Shinan-gun (483) for P. summa. Moreover, individual mullet caught from the same area also revealed remarkable differences in the metacercarial density, for example, in Shinan-gun, the density ranged from 10 to 2,314 metacercariae for H. nocens and from 4 to 3,106 metacercariae for P. summa (n = 20 mullets).
The exact survey areas were different between the present study and several previous studies (
Seo et al., 1980,
1981b;
Sohn et al., 1989,
1994). However, the results of the present study were somewhat different from those of the previous studies. In the study progressed in 1980, all mullets caught from Ganghwa-gun were found free from infections with metacercariae of
H. nocens and
P. summa (
Seo et al., 1981b). However, in the present study,
H. nocens metacercariae were found in 7 (47%) of 15 mullets from Ganghwa-gun, and all (100%) mullets from Gangwha-gun were infected with
P. summa metacercariae. In the present study, mullets from Seocheon-gun were highly infected with metacercariae of
H. nocens (73%) and
P. summa (53%), however, in a previous study, no metacercariae of these species were found in 5 mullets from an area nearby the present surveyed area (
Seo et al., 1981b). These results may represent significant changes in the ecology of these heterophyid flukes in western coastal areas of the Republic of Korea.
Moreover, the highest metacercarial density (av. 271 metacercariae for
H. nocens, and av. 483 metacercariae for
P. summa) and high infection rates (both 100%) were observed in mullets from an island of Shinan-gun, a western coastal area, which is not far from 5 km from Mokpo-shi. These results agree to a previous study (
Seo et al., 1981b), in which all mullets caught from Mokpo-shi were found infected with metacercariae of
H. nocens and
P. summa. However, metacercarial densities in that survey (
H. nocens: 52,
P. summa: 23 metacercariae per mullet) were much lower than those observed in the present study.
Similarly, in southern coastal areas, the status of metacercarial infection in fish also seems to be variable according to the time of surveys. For example, Seo et al. (
1980) reported low infection rates (
H. nocens: 0%,
P. summa: 7%) and low metacercarial densities in 14 mullets caught from Sacheon-shi. However, remarkable increases in the infection rates (100% for both species) and metacercarial densities were reported in another study in a nearby area, Samcheonpo-shi (
Sohn et al., 1989). However, in the present study, out of 17 mullets, only 8 (47%) were infected with
H. nocens metacercariae and 13 (77%) were with
P. summa metacercariae. It is suggested that the ecology, in terms of endemicity, of
H. nocens and
P. summa around Sacheon-shi may have fluctuated during the 2 decades between the 1980s and the 2000s.
In eastern coastal areas, the infection rate and density of heterophyid metacercariae in mullets were very low both in the present study and in a previous study (
Seo et al., 1981b). For example, in Yeongdeok-gun, it was reported that 1 of 3 mullets examined was infected with
H. nocens metacercariae (
Seo et al., 1981b). However, in the present study, no metacercariae of
H. nocens and
P. summa were detected in 10 mullets from Yeongdeok-gun and 5 mullets from Sokcho-shi. Only 1 of 10 mullets from Donghae-shi was infected with
P. summa metacercariae.
In the present study, the majority of
H. nocens metacercariae were found in the trunk, gill, and head of mullets, and the majority of
P. summa metacercariae were found in the gill, trunk, and intestine. These results are similar to those of previous studies. Seo et al. (
1981b) reported metacercariae of
H. nocens and
P. summa in the muscle (90.9% and 0.7%, respectively) and gill (9.1% and 99.3%) of mullets. Sohn et al. (
1989) found
P. summa metacercariae in the head of mullets, including the gill (87.4%) and viscera (12.6%), and
H. nocens metacercariae in the muscle (82.2%) and head (17.8%) of mullets caught from Haenam-gun. However, metacercarial distribution of
H. nocens in a previous study in Sacheon-shi was quite different from that in the present study;
H. nocens metacercariae were found in the head (83.6%), muscle (16.2%), and viscera (0.2%) of mullets (
Sohn et al., 1994). The reasons and factors for this difference should be elucidated.
Only a few reports have been available on the infection of gobies with heterophyid metacercariae in the Republic of Korea. Infections with
H. nocens metacercariae in gobies from Mokpo-shi, Yeochun-gun, and Haenam-gun were reported previously (
Seo et al., 1981b;
Sohn et al., 1994) and infections with
P. summa metacercariae were studied in gobies from Mokpo-shi (
Seo et al., 1981b). In the present study, it has been first shown that gobies from Muan-gun were infected with
H. nocens and
P. summa metacercariae, and that gobies from Seocheon-gun were infected with
H. nocens metacercariae. With regard to
H. nocens metacercariae, metacercarial distribution in the body of gobies varied by different reports. In the present study,
H. nocens metacercariae were detected in the gill as well as the head and trunk (muscle) of gobies. However, Sohn et al. (
1994) reported that
H. nocens metacercariae were distributed only in the muscle (67.7%) and head (32.3%) of gobies.
It is concluded that H. nocens and P. summa metacercariae are highly prevalent in mullets and gobies caught from western and southern coastal areas of the Republic of Korea. The prevalence and metacercarial density varied according to locality where mullets and gobies were caught. Attention to prevent human H. nocens and P. summa infections is required in the coastal areas of the Republic of Korea.