Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum
H H Ang,*1K L Chan,1 and J W Mak2
1School of Pharmaceutical Sciences, University of Science Malaysia, Minden, 11800, Filaria, Malaysia.
Received December 09, 1995; Accepted August 26, 1996.
Abstract
Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty- eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.
Tables
Table 1 GDH isoenzyme variants for Plasmodium falciparum isolates and clones
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