Original Article
Korean J Parasitol. 1989 Mar;27(1):23-33. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1989.27.1.23
Copyright © 1989 by The Korean Society for Parasitology
Effect of Hymenolepis nana infection on immunological responses of mice to sRBC
J K Rhee,S Y Youk and B K Park
Department of Veterinary Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 560-756, Korea.
Abstract

In an attempt to investigate the effect of Hymenolepis nana infection on immunological responses to sRBC in ICR strain of mice, cellular and humoral immune responses were chronologically monitored after sensitization with sRBC. Mice weighing about 20 g were allocated into artificial and natural infection groups. The shell-free eggs of H. nana were inoculated into mice on the day 0 (initial) and day 10 in the former group, and praziquantel (25 mg/kg/day) was administered for 3 days to the one half of the mice at the 15th day after the first inoculation and to all of the mice in natural infection group. In artificial infection group, the delayed-type hypersensitivity (DTH) to sRBC was considerably decreased on the day 10 after the first inoculation, and then elevated gradually to normal. Eosinophils in the peripheral blood increased slightly. The hemagglutinin (HA) and hemolysin (HE) titers during the early stage were shown to be more or less higher than those of control. Thereafter, the titers were returned to normal, followed by a transient decrease on the day 15 post-infection. The sRBC rosette and antibody-treated rosette-forming capacities on the day 15 post-infection were temporarily lowered but became higher thereafter. The mucosal mast cells (MMC) in the small intestine were gradually increased to make a peak on the day 10 post-infection and then maintained more or less at lower level. After praziquantel treatment, the DTH and the number of eosinophils were decreased slightly and the MMC number and sRBC rosette-forming capacity were considerably decreased. The titers of HA and HE and antibody-treated rosette-forming capacity, however, were elevated in general. In natural infection group, the DTH, the number of eosinophils, and MMC which were elevated due to H. nana infection were gradually returned to normal after praziquantel treatment. The titers of HA and HE which were decreased by parasite infection were increased to normal after the treatment. However, the capacities of sRBC rosette or antibody-treated rosette formation were maintained at low levels in spite of the treatment. These results revealed that the immune responses to sRBC were significantly activated during H. nana infection, although they were transiently decreased during the days 10-15 post-infection.

Figures


Fig. 1
Experimental procedure.

Tables


Table 1
Effect of Hymenolepis nana infection on DTH to sRBC in mice


Table 2
Effect of H. nana infection on sRBC-rosette formation of splenocytes in mice


Table 3
Effect of H. nana infection on the distribution of eosinophil in peripheral blood of mice


Table 4
Effect of H. nana infection on the distribution of mucosal mast cells in intestine of mice


Table 5
Effect of H. nana infection on HA and HE antibody responses to sRBC in mice


Table 6
Effect of H. nana infection on antibody-coated rosette formation of splenocytes to sRBC in mice

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