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Volume 61(4); November 2023

Review

Paleoparasitology research on ancient helminth eggs and larvae in the Republic of Korea
Jong-Yil Chai, Min Seo, Dong Hoon Shin
Parasites Hosts Dis 2023;61(4):345-387.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23085
Paleoparasitology is a discipline that applies existing conventional and molecular techniques to study parasites found in ancient ruins. This review focuses on the history of the discovery of parasites (mostly helminth eggs and larvae) in archaeological soil samples and mummies in Korea from the Three Kingdoms Period to the Joseon Dynasty (100 BCE-1910 CE). We also briefly review important milestones in global paleoparasitology. The helminth species reported so far in Korea included Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis (larva), Trichostrongylus sp. (larva), Paracapillaria philippinensis (syn. Capillaria philippinensis), Enterobius vermicularis, Fasciola hepatica, dicrocoeliids, Paragonimus westermani, Clonorchis sinensis, Metagonimus yokogawai, Pygidiopsis summa, Gymnophalloides seoi, Isthmiophora hortensis, Dibothriocephalus nihonkaiensis (syn. Diphyllobothrium nihonkaiense), and Taenia spp. tapeworms. The findings obtained by Korean paleoparasitologists/archaeologists have brought about deep insight into the status of helminthic infections in Korea’s past populations. Continued paleoparasitological research is essential for further understanding of ancient parasites and parasitic diseases in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • 7,094 View
  • 224 Download
  • 1 Web of Science
  • Crossref

Original Articles

Involvement of NOX2-derived ROS in human hepatoma HepG2 cell death induced by Entamoeba histolytica
Young Ah Lee, Myeong Heon Shin
Parasites Hosts Dis 2023;61(4):388-396.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23094
Entamoeba histolytica is an enteric tissue-invasive protozoan parasite causing amoebic colitis and liver abscesses in humans. Amoebic contact with host cells activates intracellular signaling pathways that lead to host cell death via generation of caspase-3, calpain, Ca2+ elevation, and reactive oxygen species (ROS). We previously reported that various NADPH oxidases (NOXs) are responsible for ROS-dependent death of various host cells induced by amoeba. In the present study, we investigated the specific NOX isoform involved in ROS-dependent death of hepatocytes induced by amoebas. Co-incubation of hepatoma HepG2 cells with live amoebic trophozoites resulted in remarkably increased DNA fragmentation compared to cells incubated with medium alone. HepG2 cells that adhered to amoebic trophozoites showed strong dichlorodihydrofluorescein diacetate (DCF-DA) fluorescence, suggesting intracellular ROS accumulation within host cells stimulated by amoebic trophozoites. Pretreatment of HepG2 cells with the general NOX inhibitor DPI or NOX2-specific inhibitor GSK 2795039 reduced Entamoeba-induced ROS generation. Similarly, Entamoeba-induced LDH release from HepG2 cells was effectively inhibited by pretreatment with DPI or GSK 2795039. In NOX2-silenced HepG2 cells, Entamoeba-induced LDH release was also significantly inhibited compared with controls. Taken together, the results support an important role of NOX2-derived ROS in hepatocyte death induced by E. histolytica.

Citations

Citations to this article as recorded by  Crossref logo
  • CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products
    Young Ah Lee, Myeong Heon Shin
    Parasites, Hosts and Diseases.2024; 62(3): 270.     CrossRef
  • Optimization of 18 S rRNA metabarcoding for the simultaneous diagnosis of intestinal parasites
    Dongjun Kang, Jun Ho Choi, Myungjun Kim, Sohyeon Yun, Singeun Oh, Myung-hee Yi, Tai-Soon Yong, Young Ah Lee, Myeong Heon Shin, Ju Yeong Kim
    Scientific Reports.2024;[Epub]     CrossRef
  • 3,612 View
  • 158 Download
  • 2 Web of Science
  • Crossref
Phagocytosis-associated genes in Acanthamoeba castellanii feeding on Escherichia coli
Min-Jeong Kim, Eun-Kyung Moon, Hye-Jeong Jo, Fu-Shi Quan, Hyun-Hee Kong
Parasites Hosts Dis 2023;61(4):397-404.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23088
Acanthamoeba species are free-living amoebae those are widely distributed in the environment. They feed on various microorganisms, including bacteria, fungi, and algae. Although majority of the microbes phagocytosed by Acanthamoeba spp. are digested, some pathogenic bacteria thrive within them. Here, we identified the roles of 3 phagocytosis-associated genes (ACA1_077100, ACA1_175060, and AFD36229.1) in A. castellanii. These 3 genes were upregulated after the ingestion of Escherichia coli. However, after the ingestion of Legionella pneumophila, the expression of these 3 genes was not altered after the consumption of L. pneumophila. Furthermore, A. castellanii transfected with small interfering RNS (siRNA) targeting the 3 phagocytosis-associated genes failed to digest phagocytized E. coli. Silencing of ACA1_077100 disabled phagosome formation in the E. coli-ingesting A. castellanii. Alternatively, silencing of ACA1_175060 enabled phagosome formation; however, phagolysosome formation was inhibited. Moreover, suppression of AFD36229.1 expression prevented E. coli digestion and consequently led to the rupturing of A. castellanii. Our results demonstrated that the ACA1_077100, ACA1_175060, and AFD36229.1 genes of Acanthamoeba played crucial roles not only in the formation of phagosome and phagolysosome but also in the digestion of E. coli.

Citations

Citations to this article as recorded by  Crossref logo
  • Phylogenomic, structural, and cell biological analyses reveal that Stenotrophomonas maltophilia replicates in acidified Rab7A-positive vacuoles of Acanthamoeba castellanii
    Javier Rivera, Julio C. Valerdi-Negreros, Diana M. Vázquez-Enciso, Fulvia-Stefany Argueta-Zepeda, Pablo Vinuesa, Michael L. Ginger, Monica Crary, Sutherland K. Maciver
    Microbiology Spectrum.2024;[Epub]     CrossRef
  • 4,517 View
  • 161 Download
  • 1 Web of Science
  • Crossref
In vitro anti-Trypanosoma cruzi activity of methanolic extract of Bidens pilosa and identification of active compounds by gas chromatography-mass spectrometry analysis
Gabriel Enrique Cázares-Jaramillo, Zinnia Judith Molina-Garza, Itza Eloisa Luna-Cruz, Luisa Yolanda Solís-Soto, José Luis Rosales-Encina, Lucio Galaviz-Silva
Parasites Hosts Dis 2023;61(4):405-417.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23069
Chagas disease, caused by Trypanosoma cruzi parasite, is a significant but neglected tropical public health issue in Latin America due to the diversity of its genotypes and pathogenic profiles. This complexity is compounded by the adverse effects of current treatments, underscoring the need for new therapeutic options that employ medicinal plant extracts without negative side effects. Our research aimed to evaluate the trypanocidal activity of Bidens pilosa fractions against epimastigote and trypomastigote stages of T. cruzi, specifically targeting the Brener and Nuevo León strains—the latter isolated from Triatoma gerstaeckeri in General Terán, Nuevo León, México. We processed the plant’s aerial parts (stems, leaves, and flowers) to obtain a methanolic extract (Bp-mOH) and fractions with varying solvent polarities. These preparations inhibited more than 90% of growth at concentrations as low as 800 μg/ml for both parasite stages. The median lethal concentration (LC50) values for the Bp-mOH extract and its fractions were below 500 μg/ml. Tests for cytotoxicity using Artemia salina and Vero cells and hemolytic activity assays for the extract and its fractions yielded negative results. The methanol fraction (BPFC3MOH1) exhibited superior inhibitory activity. Its functional groups, identified as phenols, enols, alkaloids, carbohydrates, and proteins, include compounds such as 2-hydroxy-3-methylbenzaldehyde (50.9%), pentadecyl prop-2-enoate (22.1%), and linalool (15.4%). Eight compounds were identified, with a match confirmed by the National Institute of Standards and Technology (NIST-MS) software through mass spectrometry analysis.

Citations

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  • Inhibitory Activity of Compounds Obtained from Streptomyces Against Trypanosoma cruzi
    Jorge Andrés Delgado-Garduño, Lucio Galaviz-Silva, Ma Guadalupe Rojas-Verde, Joel Horacio Elizondo-Luevano, Lidia Baylón-Pacheco, José Luis Rosales-Encina, Guadalupe Gutiérrez-Soto, Zinnia Judith Molina-Garza
    Pathogens.2025; 14(7): 638.     CrossRef
  • Nanostructured Polyurethane‐Collagen Hydrogels: Bioactive Crosslinked Networks for Enhanced Wound Healing
    María I. León‐Campos, Jesús A. Claudio‐Rizo, Luis E. Cobos‐Puc, Denis A. Cabrera‐Munguía, Anilú Rubio‐Rios, Ernesto Oyervides‐Muñoz, Angélica Velázquez‐Arrellano
    Journal of Biomedical Materials Research Part A.2025;[Epub]     CrossRef
  • Assessment of Anticancer Properties of Argemone mexicana L. and Berberine: A Comparative Study
    Joel H. Elizondo-Luevano, Ramiro Quintanilla-Licea, Imelda N. Monroy-García, Miroslava Kačániová, Uziel Castillo-Velázquez, Aldo F. Bazaldúa-Rodríguez, Lourdes M. Garza-Vega, Ángel D. Torres-Hernández, Abelardo Chávez-Montes
    Plants.2024; 13(10): 1374.     CrossRef
  • In Vitro Evaluation of Anti-Hemolytic and Cytotoxic Effects of Traditional Mexican Medicinal Plant Extracts on Human Erythrocytes and Cell Cultures
    Joel H. Elizondo-Luevano, Ramiro Quintanilla-Licea, Sandra L. Castillo-Hernández, Eduardo Sánchez-García, Minerva Bautista-Villarreal, Georgia M. González-Meza, Marcela A. Gloria-Garza, Osvelia E. Rodríguez-Luis, Maciej Ireneusz Kluz, Miroslava Kačániová
    Life.2024; 14(9): 1176.     CrossRef
  • 3,303 View
  • 138 Download
  • 5 Web of Science
  • Crossref
The detection of Toxoplasma gondii ME49 infections in BALB/c mice using various techniques
Hae-Ji Kang, Jie Mao, Min-Ju Kim, Keon-Woong Yoon, Gi-Deok Eom, Ki-Back Chu, Eun-Kyung Moon, Fu-Shi Quan
Parasites Hosts Dis 2023;61(4):418-427.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23048
Toxoplasma gondii infections are primarily diagnosed by serological assays, whereas molecular and fluorescence-based techniques are garnering attention for their high sensitivity in detecting these infections. Nevertheless, each detection method has its limitations. The toxoplasmosis detection capabilities of most of the currently available methods have not been evaluated under identical experimental conditions. This study aimed to assess the diagnostic potential of enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and immunofluorescence (IF) in BALB/c mice experimentally infected with various doses of T. gondii ME49. The detection of toxoplasmosis from sera and brain tissues was markedly enhanced in mice subjected to high infection doses (200 and 300 cysts) compared to those subjected to lower doses (10 and 50 cysts) for all the detection methods. Additionally, increased B1 gene expression levels and cyst sizes were observed in the brain tissues of the mice. Importantly, IHC, IF, and ELISA, but not RT-PCR, successfully detected T. gondii infections at the lowest infection dose (10 cysts) in the brain. These findings may prove beneficial while designing experimental methodologies for detecting T. gondii infections in mice.

Citations

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  • Spatiotemporal Diffusion, Colonization, and Antibody Responses in Susceptible C57BL/6J Mice Orally Infected with Toxoplasma gondii Cysts
    Zhao Li, Qi-Shuai Liu, Jun-Jie Hu, Cai-Qin Deng, Tao Li, Wen-Bin Zheng, Xing-Quan Zhu, Feng-Cai Zou
    Veterinary Sciences.2025; 12(3): 212.     CrossRef
  • Ivermectin Identified Using a High-Throughput Screening System Exhibits Anti-Clonorchis sinensis Activity in Rats
    Soon-Ok Lee, Hyeryon Lee, Ki Back Chu, Jianhua Li, Sung-Jong Hong, Sung Soo Kim, Joo Hwan No, Fu-Shi Quan
    Antibiotics.2025; 14(8): 837.     CrossRef
  • Recombinant vaccinia virus expressing MIC8, AMA1, or RON4 induce protection against Toxoplasma gondii ME49 strain infection
    Hae-Ji Kang, Fu-Shi Quan
    Acta Tropica.2025; 270: 107812.     CrossRef
  • Protective Efficacy Induced by Virus-like Particles Expressing Dense Granule Protein 5 of Toxoplasma gondii
    Su In Heo, Hae-Ji Kang, Jie Mao, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan
    Vaccines.2025; 13(8): 787.     CrossRef
  • Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection
    Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan
    Parasites, Hosts and Diseases.2025; 63(4): 340.     CrossRef
  • 3,088 View
  • 147 Download
  • 4 Web of Science
  • Crossref
Functional characterization of Clonorchis sinensis choline transporter
Jeong Yeon Won, Johnsy Mary Louis, Eui Sun Roh, Seok Ho Cha, Jin-Hee Han
Parasites Hosts Dis 2023;61(4):428-438.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23082
Clonorchis sinensis is commonly found in East Asian countries. Clonorchiasis is prevalent in these countries and can lead to various clinical symptoms. In this study, we used overlap extension polymerase chain reaction (PCR) and the Xenopus laevis oocyte expression system to isolate a cDNA encoding the choline transporter of C. sinensis (CsChT). We subsequently characterized recombinant CsChT. Expression of CsChT in X. laevis oocytes enabled efficient transport of radiolabeled choline, with no detectable uptake of arginine, α-ketoglutarate, p-aminohippurate, taurocholate, and estrone sulfate. Influx and efflux experiments showed that CsChT-mediated choline uptake was time- and sodium-dependent, with no exchange properties. Concentration-dependent analyses of revealed saturable kinetics consistent with the Michaelis–Menten equation, while nonlinear regression analyses revealed a Km value of 8.3 μM and a Vmax of 61.0 pmol/oocyte/h. These findings contribute to widen our understanding of CsChT transport properties and the cascade of choline metabolisms within C. sinensis.

Citations

Citations to this article as recorded by  Crossref logo
  • Functional characterization of glucose transporter 4 involved in glucose uptake in Clonorchis sinensis
    Hojong Jun, Ernest Mazigo, Wang-Jong Lee, Yun-Kyu Park, Jin-Hee Han, Seok Ho Cha
    Parasites, Hosts and Diseases.2024; 62(4): 450.     CrossRef
  • 3,512 View
  • 148 Download
  • 1 Web of Science
  • Crossref
Efficacy of recombinant enolase as a candidate vaccine against Haemaphysalis longicornis tick infestation in mice
Md. Samiul Haque, Mohammad Saiful Islam, Myung-Jo You
Parasites Hosts Dis 2023;61(4):439-448.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23075
Tick infestation causes a significant threat to human and animal health, requiring effective immunological control methods. This study aimed to investigate the potential of recombinant Haemaphysalis longicornis enolase protein for tick vaccine development. The exact mechanism of the recently identified enolase protein from the H. longicornis Jeju strain remains poorly understood. Enolase plays a crucial role in glycolysis, the metabolic process that converts glucose into energy, and is essential for the motility, adhesion, invasion, growth, and differentiation of ticks. In this study, mice were immunized with recombinant enolase, and polyclonal antibodies were generated. Western blot analysis confirmed the specific recognition of enolase by the antiserum. The effects of immunization on tick feeding and attachment were assessed. Adult ticks attached to the recombinant enolase-immunized mice demonstrated longer attachment time, increased blood-sucking abilities, and lower engorgement weight than the controls. The nymphs and larvae had a reduced attachment rate and low engorgement rate compared to the controls. Mice immunized with recombinant enolase expressed in Escherichia coli displayed 90% efficacy in preventing tick infestation. The glycolytic nature of enolase and its involvement in crucial physiological processes makes it an attractive target for disrupting tick survival and disease transmission. Polyclonal antibodies recognize enolase and significantly reduce attachment rates, tick feeding, and engorgement. Our findings indicate that recombinant enolase may be a valuable vaccine candidate for H. longicornis infection in experimental murine model.

Citations

Citations to this article as recorded by  Crossref logo
  • Comprehensive antigen identification and comparative analysis: significant approaches for controlling Haemaphysalis longicornis ticks
    Md. Samiul Haque, Bumseok Kim, Myung-Jo You
    Journal of Veterinary Science.2025;[Epub]     CrossRef
  • Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction in Haemaphysalis longicornis (Acari: Ixodidae) ticks
    Md. Samiul Haque, Mohammad Saiful Islam, Myung-Jo You
    Journal of Veterinary Science.2024;[Epub]     CrossRef
  • Targeting Plasmodium Life Cycle with Novel Parasite Ligands as Vaccine Antigens
    Shan Khan, Manas Paresh Patel, Aleem Damji Patni, Sung-Jae Cha
    Vaccines.2024; 12(5): 484.     CrossRef
  • 3,218 View
  • 151 Download
  • 3 Web of Science
  • Crossref
Brief Communications
Antiamoebic activities of flavonoids against pathogenic free-living amoebae, Naegleria fowleri and Acanthamoeba species
Hương Giang Lê, Tuấn Cường Võ, Jung-Mi Kang, Thu Hằng Nguyễn, Buyng-Su Hwang, Young-Taek Oh, Byoung-Kuk Na
Parasites Hosts Dis 2023;61(4):449-454.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23078
Free-living amoebae (FLA) rarely cause human infections but can invoke fatal infections in the central nervous system (CNS). No consensus treatment has been established for FLA infections of the CNS, emphasizing the urgent need to discover or develop safe and effective drugs. Flavonoids, natural compounds from plants and plant-derived products, are known to have antiprotozoan activities against several pathogenic protozoa parasites. The anti-FLA activity of flavonoids has also been proposed, while their antiamoebic activity for FLA needs to be emperically determined. We herein evaluated the antiamoebic activities of 18 flavonoids against Naegleria fowleri and Acanthamoeba species which included A. castellanii and A. polyphaga. These flavonoids showed different profiles of antiamoebic activity against N. fowleri and Acanthamoeba species. Demethoxycurcumin, kaempferol, resveratrol, and silybin (A+B) showed in vitro antiamoebic activity against both N. fowleri and Acanthamoeba species. Apigenin, costunolide, (‒)-epicatechin, (‒)-epigallocatechin, rosmarinic acid, and (‒)-trans-caryophyllene showed selective antiamoebic activity for Acanthamoeba species. Luteolin was more effective for N. fowleri. However, afzelin, berberine, (±)-catechin, chelerythrine, genistein, (+)-pinostrobin, and quercetin did not exhibit antiamoebic activity against the amoeba species. They neither showed selective antiamoebic activity with significant cytotoxicity to C6 glial cells. Our results provide a basis for the anti-FLA activity of flavonoids, which can be applied to develope alternative or supplemental therapeutic agents for FLA infections of the CNS.

Citations

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  • Effectiveness of phytoproducts against pathogenic free-living amoebae - A scoping and critical review paving the way toward plant-based pharmaceuticals
    Beni Jequicene Mussengue Chaúque, Thaisla Cristiane Borella da Silva, Eduardo Brittes Rott, Felipe Brittes Rott, Ana Paula Marçal Copetti Leite, Guilherme Brittes Benitez, Neuana Fernando Neuana, José Roberto Goldim, Marilise Brittes Rott, Régis Adriel Za
    Fitoterapia.2025; 182: 106404.     CrossRef
  • Unveiling phenolic content, antibacterial, and antibiofilm potential of sacha inchi (Plukenetia volubilis L.) seed shell extracts against Staphylococcus aureus
    Gadah A. Al-Hamoud, Musarat Amina, Reem Hamoud Alrashoudi, Ayesha Mateen, Farah Maqsood, Hanan M. Al-Yousef
    PeerJ.2025; 13: e19524.     CrossRef
  • A xanthone O-glucoside isolated from Iris setosa Pall. ex Link exhibits promising anti-amoebic activity against the brain-eating amoeba Naegleria fowleri
    Hương Giang Lê, Buyng Su Hwang, Ji-Su Choi, Yong Tae Jeong, Tuấn Cường Võ, Minkyoung Cho, Yeonchul Hong, Jeong Ho Kim, Young Taek Oh, Byoung-Kuk Na
    Phytomedicine.2025; 147: 157199.     CrossRef
  • From nose to neurons: The lethal journey of the brain-eating amoeba Naegleria fowleri
    Arindam Mitra, Débora Brito Goulart
    The Microbe.2025; 8: 100537.     CrossRef
  • (‒)-Epicatechin reveals amoebicidal activity against Acanthamoeba castellanii by activating the programmed cell death pathway
    Hương Giang Lê, Jung-Mi Kang, Tuấn Cường Võ, Won Gi Yoo, Yeonchul Hong, Byoung-Kuk Na
    Phytomedicine.2024; 125: 155389.     CrossRef
  • The potential of nanocomposites (patuletin-conjugated with gallic acid-coated zinc oxide) against free-living amoebae pathogens
    Ruqaiyyah Siddiqui, Bushra Khatoon, Muhammad Kawish, Sreedevi Sajeev, Shaheen Faizi, Muhammad Raza Shah, Ahmad M. Alharbi, Naveed Ahmed Khan
    International Microbiology.2024; 28(5): 929.     CrossRef
  • A Comprehensive Review on the Antibacterial, Antifungal, Antiviral, and Antiparasitic Potential of Silybin
    José Lima Pereira-Filho, Amanda Graziela Gonçalves Mendes, Carmem Duarte Lima Campos, Israel Viegas Moreira, Cinara Regina Aragão Vieira Monteiro, Suzany Hellen da Silva Soczek, Elizabeth Soares Fernandes, Rafael Cardoso Carvalho, Valério Monteiro-Neto
    Antibiotics.2024; 13(11): 1091.     CrossRef
  • 3,818 View
  • 190 Download
  • 7 Web of Science
  • Crossref
Genetic polymorphism of merozoite surface protein 1 and antifolate-resistant genes in Plasmodium falciparum from Mali and Niger
Mahaman Moustapha Lamine, Rabia Maman, Abdoul Aziz Maiga, Ibrahim Maman Laminou
Parasites Hosts Dis 2023;61(4):455-462.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23049
Since 2015, countries in the Sahel region have implemented large-scale seasonal malaria chemoprevention (SMC). However, the mass use of sulfadoxine-pyrimethamine (SP) plus amodiaquine impacts the genetic diversity of malaria parasites and their sensitivity to antimalarials. This study aimed to describe and compare the genetic diversity and SP resistance of Plasmodium falciparum strains in Mali and Niger. We collected 400 blood samples in Mali and Niger from children aged 3–59 months suspected of malaria. Of them, 201 tested positive (Niger, 111, 55.2%; Mali, 90, 44.8%). Polymorphism of merozoite surface protein 1 (msp1) genetic marker showed 201 allotypes. The frequency of the RO33 allotype was significantly higher in Niger (63.6%) than in Mali (39.3%). There was no significant difference in the frequency of the K1 and MAD20 allotypes between the 2 countries. The multiplicity of infection was 2 allotypes per patient in Mali and one allotype per patient in Niger. The prevalence of strains with the triple mutants Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H and Pfdhfr51I/Pfdhfr59R/Pfdhps437G was 18.1% and 30.2%, respectively, and 7.7% carried the quadruple mutant Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H/Pfdhps437G. Despite the significant genetic diversity of parasite populations, the level of SP resistance was comparable between Mali and Niger. The frequency of mutations conferring resistance to SP still allows its effective use in intermittent preventive treatment in pregnant women and in SMC.

Citations

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  • Preventive interventions and diagnostic testing compliance in the management of tropical infections among patients with sickle cell disease in Tanzania
    Felician Paschal Mgasa, Singeun Oh, Avelina Mgasa, Sunjoo Kang, Erasto Mbugi, Ju Yeong Kim
    Parasites, Hosts and Diseases.2025; 63(2): 147.     CrossRef
  • Micro-geographic variation in antigenic diversity of PfEBA-175 region II in asymptomatic Plasmodium falciparum infections in Tanzania
    Jadidan Hada Syahada, Wang-Jong Lee, Hojong Jun, Johnsy Mary Louis, Fadhila Fitriana, Fauzi Muh, Feng Lu, Md Atique Ahmed, Sunghun Na, Wanjoo Chun, Won Sun Park, Bo-Young Jeon, Eun-Teak Han, Jim Todd, Alphaxard Manjurano, Winifrida Kidima, Ernest Mazigo,
    Frontiers in Immunology.2025;[Epub]     CrossRef
  • 2,328 View
  • 126 Download
  • 3 Web of Science
  • Crossref
Update on the risk factors for opisthorchiasis and cholangiocarcinoma in Thailand
Sattrachai Prasopdee, Thittinan Rojthongpond, Yanwadee Chitkoolsamphan, Montinee Pholhelm, Siraphatsorn Yusuk, Junya Pattaraarchachai, Kritiya Butthongkomvong, Jutharat Kulsantiwong, Teva Phanaksri, Anthicha Kunjantarachot, Smarn Tesana, Thanakrit Sathavornmanee, Veerachai Thitapakorn
Parasites Hosts Dis 2023;61(4):463-470.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23032
This study aimed to identify the recent risk factors for Opisthorchis viverrini infection and cholangiocarcinoma (CCA) to improve disease prevention. The participants were divided into the following 3 groups based on their health status: healthy control (nonOV and nonCCA), those with O. viverrini infection (OV), and those with CCA. A questionnaire was used to explore their lifestyle and behaviors. Multivariate logistic regression and backward elimination were used to identify the significant risk factors. The results showed that the significant risk factors for both O. viverrini infection and CCA were age>50 years (odd ratio (OR)=8.44, p<0.001, 95% confidence intervals (CI) 2.98–23.90 and OR=43.47, p=0.001, 95% CI 14.71–128.45, respectively) and raw fish consumption (OR=8.48, p< 0.001, 95% CI 3.18–22.63 and OR=3.15, p=0.048, 95% CI 1.01–9.86, respectively). A history of O. viverrini infection was identified as an additional risk factor for CCA (OR=20.93, p=0.011, 95% CI 2.04–215.10). This study provided an update on the risk factors for O. viverrini infection and CCA. Asymptomatic patients with O. viverrini infection, particularly those>50 years old, should be carefully monitored to prevent CCA.

Citations

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  • Influence of knowledge, attitude and self‐efficacy on Opisthorchis viverrini and cholangiocarcinoma prevention: A One Health approach in high‐risk areas of Thailand
    Worathon Busabong, Nopparat Songserm, Somkiattiyos Woradet, Banchob Sripa
    Tropical Medicine & International Health.2025; 30(2): 99.     CrossRef
  • Targeting tumor-associated genes, immune response, and circulating tumor cells in intrahepatic cholangiocarcinoma: Therapeutic potential of Atractylodes lancea (Thunb.) DC
    Pongsakorn Martviset, Pathanin Chantree, Nisit Tongsiri, Tullayakorn Plengsuriyakarn, Kesara Na-Bangchang, Keun-Yeong Jeong,
    PLOS One.2025; 20(5): e0323732.     CrossRef
  • Large-scale epidemiology of opisthorchiasis in 21 provinces in Thailand based on diagnosis by fecal egg examination and urine antigen assay and analysis of risk factors for infection
    Kulthida Y. Kopolrat, Chanika Worasith, Phattharaphon Wongphutorn, Anchalee Techasen, Chatanun Eamudomkarn, Jiraporn Sithithaworn, Watcharin Loilome, Nisana Namwat, Attapol Titapun, Chaiwat Tawarungruang, Bandit Thinkhamrop, Samarn Futrakul, Simon D. Tayl
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Morphological studies of fly puparia (Diptera: Calliphoridae) in the soil from a Joseon Dynasty grave in Korea
In-Yong Lee, Ju Yeong Kim, Jung-Min Park, Ji Ho Seo, Bo-Young Jeon, Tai-Soon Yong, Min Seo
Parasites Hosts Dis 2023;61(4):471-475.
Published online November 28, 2023
DOI: https://doi.org/10.3347/PHD.23047
Archaeoentomological investigations were conducted on soil contents from a grave belonging to the Joseon Dynasty as part of the Urban Environment Maintenance Project (UEMP) in Cheongjin 12–16 dong (districts), Jongno-gu, Seoul, Korea, from December 01, 2008 to February 19, 2011. A total of 28 insect puparia with hard shells of the common green bottle fly Lucilia sericata were identified in the soil. Evidence suggested that the corpse was placed outside for some days instead of being buried immediately after death. This is the first report of fly puparia in soil samples from a tomb of the Joseon Dynasty during 16–17 AD in Korea. Our findings may help determine the timeframe of burial and offer archaeological insights into the funerary customs of the period.
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