Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2, Tvα-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of Tvα-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-Tvα-actinin 2 antibodies indicated its presence in the secreted proteins of T. vaginalis. ELISA was employed to measure cytokine production by vaginal epithelial cells, prostate cells, mouse dendritic cells (DCs), or T cells stimulated with T. vaginalis or Tvα-actinin 2 protein. Both T. vaginalis and rTvα-actinin 2 induced cytokine production from epithelial cell lines, including IL-10. Moreover, CD4+CD25- regulatory T cells (Treg cells) incubated with rTvα-actinin 2-treated DCs produced high levels of IL-10. These data indicate that Tvα-actinin 2 modulates immune responses via IL-10 production by Treg cells.
Citations
Citations to this article as recorded by
Identification and Characterization of α-Actinin 1 of Histomonas meleagridis and Its Potential Vaccine Candidates against Histomonosis Dandan Liu, Chen Chen, Qiaoguang Chen, Shuang Wang, Zaifan Li, Jie Rong, Yuming Zhang, Zhaofeng Hou, Jianping Tao, Jinjun Xu Animals.2023; 13(14): 2330. CrossRef
Surface‐enhanced Raman scattering of secretory proteins for the cytotoxicity analysis of low‐dose doxorubicin Mengmeng Zheng, Siqi Gao, Yamin Lin, Yating Lin, Zufang Huang, Shusen Xie, Yun Yu, Juqiang Lin Journal of Raman Spectroscopy.2020; 51(11): 2217. CrossRef
Vasoactive intestinal peptide is required in the maintenance of immune regulatory competency of immune regulatory monocytes L Guan, D Yu, G-H Wu, H-J Ning, S-D He, S-S Li, T-Y Hu, G Yang, Z-Q Liu, H-Q Yu, X-Z Sun, Z-G Liu, P-C Yang Clinical and Experimental Immunology.2019; 196(2): 276. CrossRef
First
Prev
