The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant α-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of α-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia.
Citations
Citations to this article as recorded by
Genetic diversity and molecular diagnosis of Giardia Yankai Chang, Junqiang Li, Longxian Zhang Infection, Genetics and Evolution.2023; 113: 105482. CrossRef
Tubulin as a potential molecular target for resveratrol in Giardia lamblia trophozoites, in vitro and in silico approaches José Roberto Vargas-Villanueva, Filiberto Gutiérrez-Gutiérrez, Mariana Garza-Ontiveros, Sendar Daniel Nery-Flores, Lizeth Guadalupe Campos-Múzquiz, Dagoberto Vazquez-Obregón, Raul Rodriguez-Herrera, Lissethe Palomo-Ligas Acta Tropica.2023; 248: 107026. CrossRef
Phosphorylation of Serine 148 in Giardia lamblia End‐binding 1 Protein is Important for Cell Division Juri Kim, Hye‐Yeon Lee, Kyu‐Ho Lee, Soon‐Jung Park Journal of Eukaryotic Microbiology.2017; 64(4): 464. CrossRef
Host defences against Giardia lamblia G. Lopez‐Romero, J. Quintero, H. Astiazarán‐García, C. Velazquez Parasite Immunology.2015; 37(8): 394. CrossRef
Characterization of Microtubule-Binding and Dimerization Activity of Giardia lamblia End-Binding 1 Protein Juri Kim, Sara Nagami, Kyu-Ho Lee, Soon-Jung Park, Ira J. Blader PLoS ONE.2014; 9(5): e97850. CrossRef
Zoonotic potential of Giardia Una Ryan, Simone M. Cacciò International Journal for Parasitology.2013; 43(12-13): 943. CrossRef
Identification of Antigenic Proteins in Trichomonas vaginalis Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park The Korean Journal of Parasitology.2011; 49(1): 79. CrossRef
Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA.
Citations
Citations to this article as recorded by
Intraspecific variability in several isolates of Philasterides dicentrarchi (syn. Miamiensis avidus), a scuticociliate parasite of farmed turbot Belén Budiño, Jesús Lamas, María P. Pata, Juan A. Arranz, Manuel L. Sanmartín, José Leiro Veterinary Parasitology.2011; 175(3-4): 260. CrossRef
Analysis of Internal Transcribed Spacer 1 Sequences of Pneumocystis jiroveci from Clinical Specimens Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee Chonnam Medical Journal.2008; 44(2): 82. CrossRef
First
Prev
