Eosinophil degranulation plays a crucial role in tissue inflammatory reactions associated with helminth parasitic infections and allergic diseases. Paragonimus westermani, a lung fluke causing human paragonimiasis, secretes a large amount of cysteine proteases, which are involved in nutrient uptake, tissue invasion, and modulation of hos's immune responses. There is, however, limited information about the response of eosinophils to direct stimulation by cysteine proteases (CP) secreted by P. westermani. In the present study, we tested whether degranulation and superoxide production from human eosinophils can be induced by stimulation of the 2 CP (27 kDa and 28 kDa) purified from excretory-secretory products (ESP) of P. westermani newly excysted metacercariae (PwNEM). A large quantity of eosinophil-derived neurotoxin (EDN) was detected in the culture supernatant when human eosinophils isolated from the peripheral blood were incubated with the purified 27 kDa CP. Furthermore, the 27 kDa CP induced superoxide anion production by eosinophils in time- and dose-dependent manners. In contrast, the purified 28 kDa CP did not induce superoxide production and degranulation. These findings suggest that the 27 kDa CP secreted by PwNEM induces superoxide production and degranulation of human eosinophils, which may be involved in eosinophil-mediated tissue inflammatory responses during the larval migration in human paragonimiasis.
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Massive secretions in paragonimiasis pleural effusion: a new finding concerning clinical recognition and treatment Yunxuan Zhang, Yimin Xie, Yarui Luo, Shuang Xiang, Wen Zhong, Na Wu, Meng Chen, Xiaojiao Zhou, Shulei Peng, Qiang Chen European Journal of Clinical Microbiology & Infectious Diseases.2023; 42(4): 493. CrossRef
Downregulated RPS-30 in Angiostrongylus cantonensis L5 plays a defensive role against damage due to oxidative stress Wei-Wei Sun, Xiu-Mei Yan, Qing Shi, Yuan-Jiao Zhang, Jun-Ting Huang, Hui-Cong Huang, Hong-Fei Shi, Bao-Long Yan Parasites & Vectors.2020;[Epub] CrossRef
Molecular and immunological characterization of cathepsin L-like cysteine protease of Paragonimus pseudoheterotremus Tippayarat Yoonuan, Supaporn Nuamtanong, Paron Dekumyoy, Orawan Phuphisut, Poom Adisakwattana Parasitology Research.2016; 115(12): 4457. CrossRef
Conservation and diversification of the transcriptomes of adult Paragonimus westermani and P. skrjabini Ben-wen Li, Samantha N. McNulty, Bruce A. Rosa, Rahul Tyagi, Qing Ren Zeng, Kong-zhen Gu, Gary J. Weil, Makedonka Mitreva Parasites & Vectors.2016;[Epub] CrossRef
Systems Biology Studies of Adult Paragonimus Lung Flukes Facilitate the Identification of Immunodominant Parasite Antigens Samantha N. McNulty, Peter U. Fischer, R. Reid Townsend, Kurt C. Curtis, Gary J. Weil, Makedonka Mitreva, Aaron R. Jex PLoS Neglected Tropical Diseases.2014; 8(10): e3242. CrossRef
Eosinophilic Pneumonias Praveen Akuthota, Peter F. Weller Clinical Microbiology Reviews.2012; 25(4): 649. CrossRef
Eosinophil and Tissue-invasive Parasitic Helminth Myeong Heon Shin Hanyang Medical Reviews.2010; 30(3): 238. CrossRef
Eosinophil-Mediated Tissue Inflammatory Responses in Helminth Infection Myeong Heon Shin, Young Ah Lee, Duk-Young Min The Korean Journal of Parasitology.2009; 47(Suppl): S125. CrossRef
The global cysteine peptidase landscape in parasites Holly J. Atkinson, Patricia C. Babbitt, Mohammed Sajid Trends in Parasitology.2009; 25(12): 573. CrossRef
Parasitic helminths: a pharmacopeia of anti-inflammatory molecules M. J. G. JOHNSTON, J. A. MacDONALD, D. M. McKAY Parasitology.2009; 136(2): 125. CrossRef
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