Parasites, Hosts and Diseases

"AGS"

Original Articles

Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae: Hae Kyung Park, Seong Won Chang, Se Won Kang, Min Kyoung Cho, Sun Hee Choi, Yeon Chul Hong, Yong Seok Lee, Hae Jin Jeong, Hak Sun Yu; Korean J Parasitol 2008;46(2):59-63.
Published online June 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.2.59

Abstract
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In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

Citations

Citations to this article as recorded by

Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage
Bin Tang, Mingyuan Liu, Libo Wang, Shenye Yu, Haining Shi, Pascal Boireau, Vasile Cozma, Xiuping Wu, Xiaolei Liu
Parasites & Vectors.2015;[Epub] CrossRef
Molecular characterization of 45kDa aspartic protease of Trichinella spiralis
Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
Veterinary Parasitology.2012; 190(3-4): 510. CrossRef
Protease-Activated Receptor 2 Is Involved in Th2 Responses againstTrichinella spiralisInfection
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu
The Korean Journal of Parasitology.2011; 49(3): 235. CrossRef
Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
Veterinary Parasitology.2009; 164(2-4): 242. CrossRef

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In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line: Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu; Korean J Parasitol 2004;42(1):27-34.
Published online March 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.1.27

Abstract
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We investigated the optimal culture conditions for Cryptosporidium muris in a human stomach adenocarcinoma (AGS) cell line by determining the effects of medium pH and of selected supplements on the development of C. muris. The optimum pH of the culture medium required for the development of C. muris was determined to be 6.6. The number of parasites significantly increased during cultivation for 72 hr (p < 0.05) at this level. On the other hand, numbers decreased linearly after 24 hr of incubation at pH 7.5. When cultured in different concentrations of serum, C. muris in media containing 5% FBS induced 4-7 times more parasites than in 1% or 10% serum. Of the six medium supplements examined, only 1 mM pyruvate enhanced the number of C. muris in vitro. Transmission electron microscopic observation showed the developmental stages of C. muris in the cytoplasm of the cells, not in an extracytoplasmic location. The growth of C. muris in AGS cells provides a means of investigating its biological characteristics and of testing its response to therapeutic agents. However, a more optimized culture system is needed for the recovery of oocysts on a large scale in vitro.

Citations

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A Scoping Review of Changing Climate and Weather Connections to Waterborne Pathogens in Coastal Environments
Bailey M. Magers, Kyle D. Brumfield, Moiz Usmani, Rita R. Colwell, Antarpreet S. Jutla
Global Change Biology.2025;[Epub] CrossRef
Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans
Janka Melicherová, Lada Hofmannová, Andrea Valigurová
European Journal of Protistology.2018; 62: 101. CrossRef
The fine structure of sexual stage development and sporogony ofCryptosporidium parvumin cell-free culture
HEBATALLA M. ALDEYARBI, PANAGIOTIS KARANIS
Parasitology.2016; 143(6): 749. CrossRef
Morphology and infectivity of virus that persistently caused infection in an AGS cell line
Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno, Tomohiko Takasaki, Kouichi Sano
Medical Molecular Morphology.2011; 44(4): 213. CrossRef
Evolution of Cryptosporidium in vitro culture
P. Karanis, H.M. Aldeyarbi
International Journal for Parasitology.2011; 41(12): 1231. CrossRef
Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells
Liang Wu, Sheng-xia Chen, Xu-gan Jiang, Yu-juan Shen, Zhao-xi Lu, Guo-hua Tu, Xing-li Fu, Jian-ping Cao
Parasitology Research.2009; 105(5): 1419. CrossRef

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Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi: Hyun-Hee Kong, Mee-Yeul Hwang, Hyo-Kyung Kim, Dong-Il Chung; Korean J Parasitol 2001;39(2):151-160.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.151

Abstract
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Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes enconding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.

Citations

Citations to this article as recorded by

Construction of EST Database for Comparative Gene Studies of Acanthamoeba
Eun-Kyung Moon, Joung-Ok Kim, Ying-Hua Xuan, Young-Sun Yun, Se Won Kang, Yong Seok Lee, Tae-In Ahn, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2009; 47(2): 103. CrossRef
Acanthamoeba Interaction with Extracellular Matrix Glycoproteins: Biological and Biochemical Characterization and Role in Cytotoxicity and Invasiveness
BRUNO DA ROCHA‐AZEVEDO, MELISSA JAMERSON, GUY A. CABRAL, FERNANDO C. SILVA‐FILHO, FRANCINE MARCIANO‐CABRAL
Journal of Eukaryotic Microbiology.2009; 56(3): 270. CrossRef
Sampling Gene Diversity Across the Supergroup Amoebozoa: Large EST Data Sets from Acanthamoeba castellanii, Hartmannella vermiformis, Physarum polycephalum, Hyperamoeba dachnaya and Hyperamoeba sp.
Russell F. Watkins, Michael W. Gray
Protist.2008; 159(2): 269. CrossRef
Anisakis simplex: Analysis of expressed sequence tags (ESTs) of third-stage larva
Hak Sun Yu, Sang Kyun Park, Keun Hee Lee, Sun Joo Lee, Sun Hee Choi, Mee Sun Ock, Hae Jin Jeong
Experimental Parasitology.2007; 117(1): 51. CrossRef
Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba
Joanna It-itan Alafag, Eun-Kyung Moon, Yeon-Chul Hong, Dong-Il Chung, Hyun-Hee Kong
The Korean Journal of Parasitology.2006; 44(4): 331. CrossRef
Acanthamoeba healyi: Molecular cloning and characterization of a coronin homologue, an actin-related protein
Eleonor T. Baldo, Eun-Kyung Moon, Hyun-Hee Kong, Dong-Il Chung
Experimental Parasitology.2005; 110(2): 114. CrossRef

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In vitro infection of Cryptosporidium parvum to four different cell lines: Jae-Ran Yu, Sung-Don Choi, Young-Wook Kim; Korean J Parasitol 2000;38(2):59-64.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.59

Abstract
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To determine a suitable condition for in vitro infection model of Cryptosporidium parvum, four different cell lines, AGS, MDCK, HCT-8 and Caco-2, were used as host cell lines which were cultured at various concentrations of added supplements. These supplement include fetal bovine serum (FBS), sodium choleate, ascorbic acid, folic acid, calcium pantothenate, para-aminobenzoic acid and pyruvate and their effects on the cell lines which were infected with C. parvum were evaluated. The results of this study showed that the AGS cell line was most susceptible to C. parvum whereas the Caco-2 cells appeared to be least susceptible to C. parvum. In regards to the serum condition, 10% FBS was suitable for the growth of AGS and HCT-8 cells, and 1% FBS was good for the growth of the MDCK cells when they were inoculated with C. parvum. Vitamines had a positive effect on the AGS cells, and pyruvate also showed positive effects on all of the cell lines except for Caco-2. Modified medium for each cell line was prepared by adding appropriate amounts of each supplement which resulted in the highest parasite infection number. Modified media increased the number of parasites infected on AGS cells to 2.3-fold higher when compared to the control media. In this study, we found that the AGS cell line was a suitable host model for evaluating C. parvum in vitro study and the media contents for the optimal infection conditions were suggested.

Citations

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Novel organoids and ex vivo models for advancing poultry coccidiosis research
Phoebe Yuen Ka Chan, Bernat Marti-Garcia, Virginia Marugan-Hernandez
Parasitology.2025; : 1. CrossRef
In vitro and in vivo anti-Cryptosporidium and anti-inflammatory effects of Aloe vera gel in dexamethasone immunosuppressed mice
Alyaa Farid, Aya Tawfik, Basil Elsioufy, Gehan Safwat
International Journal for Parasitology: Drugs and Drug Resistance.2021; 17: 156. CrossRef
Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans
Janka Melicherová, Lada Hofmannová, Andrea Valigurová
European Journal of Protistology.2018; 62: 101. CrossRef
A new in vitro model using small intestinal epithelial cells to enhance infection of Cryptosporidium parvum
Eunice A. Varughese, Christina L. Bennett-Stamper, Larry J. Wymer, Jagjit S. Yadav
Journal of Microbiological Methods.2014; 106: 47. CrossRef
Apicomplexan infections in the gut
C. L. Wilhelm, F. Yarovinsky
Parasite Immunology.2014; 36(9): 409. CrossRef
Evolution of Cryptosporidium in vitro culture
P. Karanis, H.M. Aldeyarbi
International Journal for Parasitology.2011; 41(12): 1231. CrossRef
Morphology and infectivity of virus that persistently caused infection in an AGS cell line
Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno, Tomohiko Takasaki, Kouichi Sano
Medical Molecular Morphology.2011; 44(4): 213. CrossRef
Determining GFP Activity in HCT-8 Cells Infected withCryptosporidium Parvumby Reverse Transcription Polymerase Chain Reaction and Nested PCR
Z. Koloren
Biotechnology & Biotechnological Equipment.2010; 24(3): 2009. CrossRef
Transient expression of red and yellow fluorescent protein vectors in HCT-8 cells ınfected with Cryptosporidium parvum
Zeynep Koloren, Sadik Dinçer
Parasitology Research.2009; 105(4): 1023. CrossRef
In vitro development of Cryptosporidium parvum in serum-free media
K.M. Woods, S.J. Upton
Letters in Applied Microbiology.2007; 44(5): 520. CrossRef
Cryptosporidium p30, a Galactose/N-Acetylgalactosamine-specific Lectin, Mediates Infection in Vitro
Najma Bhat, Angela Joe, Mercio PereiraPerrin, Honorine D. Ward
Journal of Biological Chemistry.2007; 282(48): 34877. CrossRef
In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line
Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu
The Korean Journal of Parasitology.2004; 42(1): 27. CrossRef
MICROBIAL ADHESION OF CRYPTOSPORIDIUM PARVUM SPOROZOITES: PURIFICATION OF AN INHIBITORY LIPID FROM BOVINE MUCOSA
Julie K. Johnson, Joann Schmidt, Howard B. Gelberg, Mark S. Kuhlenschmidt
Journal of Parasitology.2004; 90(5): 980. CrossRef
IcsB, secreted via the type III secretion system, is chaperoned by IpgA and required at the post‐invasion stage of Shigella pathogenicity
Michinaga Ogawa, Toshihiko Suzuki, Ichiro Tatsuno, Hiroyuki Abe, Chihiro Sasakawa
Molecular Microbiology.2003; 48(4): 913. CrossRef
The effect of microfilament inhibitor on the Cryptosporidium infection in vitro
Jae-Ran Yu, Sung-Don Choi
The Korean Journal of Parasitology.2000; 38(4): 257. CrossRef