Parasites, Hosts and Diseases

"Abdukhakim Khajibaev"

Original Articles

Prevalence of Serum IgG Antibodies to Cystic Echinococcus Antigen among Patients in an Uzbekistan Emergency Hospital: Se Jin Park, Sung Sik Han, Khikmat Anvarov, Abdukhakim Khajibaev, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2015;53(6):699-703.
Published online December 31, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.6.699

Abstract
PDF

Cystic echinococcosis (CE) is one of the most widespread zoonotic helminthiases, which can last an asymptomatic infection for several years. The purpose of this study was to demonstrate serum antibody prevalence of CE among asymptomatic people in Uzbekistan using ELISA. A total of 2,547 serum samples were collected, 66 from confirmed CE patients and 2,481 of patients with other diseases than CE at a hospital in Tashkent, Uzbekistan. The serum samples were screened for CE specific IgG antibodies by ELISA using cystic fluid antigen obtained from sheep. The serum antibody positive rate was 89.4% (59/66) in CE and 3.6% (89/2,481) in other disease patients. The present ELISA recognized 89.4% sensitivity and 96.4% specificity. The ELISA absorbance of positive samples was distributed 0.271-0.971 for CE and 0.273-0.887 for other disease patients. The other disease patients with high absorbance over 0.3 were 50 (2.0%) who were presumed to be active CE patients. The patients in their 40s showed the highest positive rate of 5.2% (P=0.181), and women were 4.4% while men were 3.1% positive (P=0.136). The data confirmed that there are many asymptomatic patients of CE in Tashkent. It is indicated that CE is an endemic disease of public health importance in Uzbekistan.

Citations

Citations to this article as recorded by

Comparison of Methods in the Serologic Diagnosis of Cystic Echinococcosis
Sidre Erganis, Fakhriddin Sarzhanov, Funda Doğruman Al, Kayhan Cağlar
Acta Parasitologica.2024; 69(2): 1122. CrossRef
The echinococcoses in Asia: The present situation
Akira Ito, Christine M. Budke
Acta Tropica.2017; 176: 11. CrossRef
Kistik Ekinokokoz’un serolojik tanısında Indirekt Hemaglütinasyon, İndirekt Floresan Antikor ve Enzim İmmuno Assay testlerinin etkinliğinin değerlendirilmesi
Sadık AKGÜN, Hakan Sezgin SAYİNER, Tekin KARSLIGİL
Journal of Contemporary Medicine.2017;[Epub] CrossRef

8,288 View
85 Download
3 Web of Science
Crossref

Serodiagnosis of Echinococcosis by ELISA Using Cystic Fluid from Uzbekistan Sheep: Yan Jin, Khikmat Anvarov, Abdukhakim Khajibaev, Samin Hong, Sung-Tae Hong; Korean J Parasitol 2013;51(3):313-317.
Published online June 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.3.313

Abstract
PDF

According to increase of travel, the cases of imported echinococcosis have been increasing in Korea. The present study was undertaken to develop a serodiagnostic system for echinococcosis in Korea. For diagnosis of echinococcosis, the fluid of Echinococcus granulosus hydatid cysts was collected from naturally infected sheep in Uzbekistan. Also serum samples of infected patients who were surgically confirmed were collected in a hospital in Tashkent, Uzbekistan. According to the absorbance of 59 echinococcosis positive and 39 negative control serum samples, the cut-off value was determined as 0.27. The sensitivity and specificity of ELISA with hydatid fluid antigen were 91.5% and 96%, respectively. The antigen cross-reacted with the serum of some cysticercosis or clonorchiasis patients. However, immunoblot analysis on the cystic fluid recognized antigenic proteins of 7-, 16-, and 24-kDa bands in their dominant protein quantity and strong blotting reactivity. In conclusion, the present ELISA system using hydatid cyst fluid antigen from Uzbekistan sheep is sensitive and specific for diagnosis of echinococcosis cases.

Citations

Citations to this article as recorded by

Delayed Diagnosis of Imported Cystic Echinococcosis and Successful Treatment With Percutaneous Drainage and Albendazole in Korea: A Case Report
Won Jun Choi, Hanna Jin, Hyeon Jae Jo, Chan Mi Lee, Chang Kyung Kang, Pyoeng Gyun Choe, Wan Beom Park, Nam Joong Kim, Min-Ho Choi
Journal of Korean Medical Science.2025;[Epub] CrossRef
Human and camel cystic echinococcosis – a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification
A. Maher, N. I. Toaleb, R. M. Shaapan, D. Aboelsoued, M. B. Salman, S. Zaky
Veterinary Research Communications.2024; 48(4): 2193. CrossRef
Past and present of diagnosis of echinococcosis: A review (1999–2021)
Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Sadiq Khan, Muhammad Saqib, Warda Qamar, Li Li, Bao-Quan Fu, Hong-Bin Yan, Wan-Zhong Jia
Acta Tropica.2023; 243: 106925. CrossRef
A Novel Designed Sandwich ELISA for the Detection of Echinococcus granulosus Antigen in Camels for Diagnosis of Cystic Echinococcosis
Nagwa I. Toaleb, Dina Aboelsoued, Kadria N. Abdel Megeed, Sahar Hussein Abdalla Hekal
Tropical Medicine and Infectious Disease.2023; 8(8): 400. CrossRef
A simple and efficient purification method of native immunoreactive antigen for diagnosis of camel hydatidosis
Nagwa I. Toaleb, Mohamed S. Helmy, Eman E. El Shanawany, Eman H. Abdel-Rahman
Veterinary World.2020; 13(1): 141. CrossRef
Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
Liu Tianli, Wang Xifeng, Tian Zhenzhong, Wang Lixia, Zhang Xingxing, Qiao Jun, Meng Qingling, Gong Shasha, Chen Ying, Cai Xuepeng
The Korean Journal of Parasitology.2019; 57(1): 61. CrossRef
An Imported Case of Disseminated Echinococcosis in Korea
Dong Hoon Shin, Hae Chan Jo, Jeong-Han Kim, Kang Il Jun, Wan Beom Park, Nam-Joong Kim, Min-Ho Choi, Chang Kyung Kang, Myoung-don Oh
The Korean Journal of Parasitology.2019; 57(4): 429. CrossRef
Serological diagnosis of cystic echinococcosis in cattle
Aitbay K. Bulashev, Zhanbolat A. Suranshiev, Orken S. Akibekov, Zhanara Zh. Akanova, Gulmira A. Abulgazimova
Folia Parasitologica.2017;[Epub] CrossRef
Diagnostic value of semi-purified antigens of hydatid cyst fluid in human cystic echinococcosis
Wissal Iraqi
Acta Parasitologica.2016;[Epub] CrossRef
Presumptive case of sparganosis manifesting as a hepatic mass: A case report and literature review
Gyeong Deok Jo, Jae Young Lee, Sung-Tae Hong, Jung Hoon Kim, Joon Koo Han
World Journal of Radiology.2016; 8(10): 846. CrossRef
Prevalence of Serum IgG Antibodies to Cystic <i>Echinococcus</i> Antigen among Patients in an Uzbekistan Emergency Hospital
Se Jin Park, Sung Sik Han, Khikmat Anvarov, Abdukhakim Khajibaev, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2015; 53(6): 699. CrossRef
Infection Status of Hydatid Cysts in Humans and Sheep in Uzbekistan
Sung-Tae Hong, Yan Jin, Khikmat Anvarov, Abdukhakim Khadjibaev, Samin Hong, Yusufjon Ahmedov, Utkir Otaboev
The Korean Journal of Parasitology.2013; 51(3): 383. CrossRef