Parasites, Hosts and Diseases

"Antibodies"

Original Articles

Bile duct changes in rats reinfected with Clonorchis sinensis: Dongil Choi, Sung-Tae Hong, Shunyu Li, Byung-Suk Chung, Jae Hoon Lim, Soon-Hyung Lee; Korean J Parasitol 2004;42(1):7-17.
Published online March 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.1.7

Abstract
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This study describes an evaluation of the sonographic, cholangiographic, pathological, and immunological findings, and the protective effect shown by rats reinfected with Clonorchis sinensis. Eight experimental rat groups were, namely, a normal control, a primary infection control, a reinfection I (reinfection 7 week after treatment following 3-week infection), a reinfection II (reinfection 2 week after treatment following 8-week infection), a reinfection III (exploration of the intrahepatic bile ducts 1 week after reinfection 4 week after treatment following 4-week infection), a superinfection, a secondary infection control, and an infection following immunization group. Sonographic and cholangiographic findings showed moderate or marked dilatation of the bile duct confluence in the primary infection control, reinfection II, and secondary infection control groups. Juvenile worms survived in the intrahepatic bile ducts 1 week after reinfection following treatment in the reinfection III group. It was concluded that reinfecting juvenile worms found during the first week following reinfection failed to survive or grow further. Anatomical, pathophysiological, or immunological changes may induce protection from reinfection in rats.

Citations

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Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid-enhanced T1 mapping to evaluate liver damage caused by Clonorchis sinensis
Yanhan Xiang, Ningqin Li, Zhongyang Zhou, Jing Fang, Jinyuan Liao
Radiology of Infectious Diseases.2021; 8(3): 108. CrossRef
Perturbations of Metabolomic Profiling of Spleen From Rats Infected With Clonorchis sinensis Determined by LC-MS/MS Method
Xiaoli Zhang, Xinyi Hu, Rui Chen, Beibei Sun, Yannan Gao, Shanshan Duan, Liyan Liu, Su Han
Frontiers in Molecular Biosciences.2020;[Epub] CrossRef
The Role of Stroma in Cholangiocarcinoma: The Intriguing Interplay between Fibroblastic Component, Immune Cell Subsets and Tumor Epithelium
Alessandra Gentilini, Mirella Pastore, Fabio Marra, Chiara Raggi
International Journal of Molecular Sciences.2018; 19(10): 2885. CrossRef
In-vivo monitoring of development of cholangiocarcinoma induced with C. sinensis and N-nitrosodimethylamine in Syrian golen hamsters using ultrasonography and magnetic resonance imaging: a preliminary study
Hyunsik Woo, Joon Koo Han, Jung Hoon Kim, Sung-Tae Hong, Md. Hafiz Uddin, Ja-June Jang
European Radiology.2017; 27(4): 1740. CrossRef
Oxidative stress-mediated mouse liver lesions caused by Clonorchis sinensis infection
Sejung Maeng, Hye Won Lee, Qudsia Bashir, Tae Im Kim, Sung-Jong Hong, Tae Jin Lee, Woon-Mok Sohn, Byoung-Kuk Na, Tong-Soo Kim, Jhang Ho Pak
International Journal for Parasitology.2016; 46(3): 195. CrossRef
Cell Death and Proliferation after Treatment and Reinfection of Clonorchis sinensis in the Sprague-Dawley Rat Bile Duct
Byoung-Hoon Min, Ka-Young Ahn, Haeng-Sook Lee, Soo-Jin Kim, Kyoung-Hwan Joo
Applied Microscopy.2015; 45(2): 80. CrossRef
Recent Advances in Serodiagnosis for Clonorchiasis
Tae Im Kim, Sung-Jong Hong
Hanyang Medical Reviews.2010; 30(3): 232. CrossRef
Fas/FasL-dependent apoptosis of hepatocytes induced in rat and patients with Clonorchis sinensis infection
Xiaoli Zhang, Zhanfeng Jin, Rong Da, Yunxia Dong, Wuqi Song, Xiaobei Chen, Qi Huang, Hong Ling, Yang Che, Yihong Li, Fengmin Zhang
Parasitology Research.2008; 103(2): 393. CrossRef
Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2008; 46(1): 17. CrossRef
Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2008; 102(6): 1111. CrossRef
Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α
Tae Yun Kim, Pyo Yun Cho, Jong Won Na, Sung-Jong Hong
Parasitology Research.2007; 101(6): 1557. CrossRef
Clonorchis sinensis: molecular cloning and localization of myosin regulatory light chain
Yeong-Deok Kwon, Pyo Yun Cho, Sung-Jong Hong
Parasitology Research.2005; 97(1): 21. CrossRef

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Toxoplasma gondii: Ultrastructural localization of specific antigens and inhibition of intracellular multiplication by monoclonal antibodies: Boo-Young Lee, Myoung-Hee Ahn, Hyun-Chul Kim, Duk-Young Min; Korean J Parasitol 2001;39(1):67-75.
Published online March 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.1.67

Abstract
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This experiment was focused on the characterization of anti-Toxoplasma monoclonal antibodies (mAbs) and the effect of mAbs on the parasite invasion of mouse peritoneal macrophages. Twenty eight mAbs including M110, M556, R7A6 and M621 were characterized by Ab titer, immunoglobulin isotyping and western blot pattern. Antibody titer (optical density) of 4 mAbs, M110, M556, R7A6 and M621, were 0.53, 0.67, 0.45 and 0.39 (normal mouse serum; 0.19) with the same IgG1 isotypes shown by Enzyme-linked immunosorbent assay (ELISA). Western blot analysis showed that M110, M556, R7A6 and M621 reacted with the 33 kDa (p30), 31 kDa (p28), 43 kDa and 36 kDa protein. Immunogold labelling of mAbs M110, M556, R7A6 and M621 reacted with the surface membrane, dense granules and parasitophorous vacuolar membrane (PVM), rhoptries and cytoplasm of tachyzoite, respectively. For in vitro assay, preincubation of tachyzoites with four mAbs, M110, M556, R7A6 and M621 resulted in the decrease of the number of infected macrophages (p < 0.05) and the suppression of parasite multiplication at 18 h postinfection. Four monoclonal antibodies including M110 (SAG1) were found to have an important role in the inhibition of macrophage invasion and T. gondii multiplication in vitro, and these mAbs may be suitable for vaccine candidates, diagnostic kit and for chemotherapy.

Citations

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Engineering and Functional Evaluation of Neutralizing Antibody Fragments Against Congenital Toxoplasmosis
Anne di Tommaso, Matthieu O Juste, Zineb Lakhrif, Marie-Noëlle Mévélec, Coraline Borowczyk, Pierre Hammeni, Guillaume Désoubeaux, Nathalie Van Langendonck, Françoise Debierre-Grockiego, Nicolas Aubrey, Isabelle Dimier-Poisson
The Journal of Infectious Diseases.2021; 224(4): 705. CrossRef
Molecular cloning and characterization ofNcROP2Fam-1, a member of the ROP2 family of rhoptry proteins inNeospora caninumthat is targeted by antibodies neutralizing host cell invasionin vitro
FERIAL ALAEDDINE, ANDREW HEMPHILL, KARIM DEBACHE, CHRISTOPHE GUIONAUD
Parasitology.2013; 140(8): 1033. CrossRef
Toxoplasma gondii: Ultrastructure study of the entry of tachyzoites into mammalian cells
Fawzia H. Toulah, Saedia A. Sayed Al-Ahl, Dawlat M. Amin, Mona H. Hamouda
Saudi Journal of Biological Sciences.2011; 18(2): 151. CrossRef
Neospora caninum: Comparative gene expression profiling of Neospora caninum wild type and a temperature sensitive clone
Robert W. Li, Wenbin Tuo
Experimental Parasitology.2011; 129(4): 346. CrossRef
Generation of a Neutralizing Human Monoclonal Antibody Fab Fragment to Surface Antigen 1 ofToxoplasma gondiiTachyzoites
Yong-Feng Fu, Meng Feng, Kenji Ohnishi, Tamon Kimura, Johbu Itoh, Xun-Jia Cheng, Hiroshi Tachibana, J. H. Adams
Infection and Immunity.2011; 79(1): 512. CrossRef
Multiplex PCR-aided Differential Diagnosis of Taeniid Species

Journal of Life Science.2010; 20(6): 955. CrossRef
Rab11A-Controlled Assembly of the Inner Membrane Complex Is Required for Completion of Apicomplexan Cytokinesis
Carolina Agop-Nersesian, Bernina Naissant, Fathia Ben Rached, Manuel Rauch, Angelika Kretzschmar, Sabine Thiberge, Robert Menard, David J. P. Ferguson, Markus Meissner, Gordon Langsley, L. David Sibley
PLoS Pathogens.2009; 5(1): e1000270. CrossRef
Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo
Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2001; 39(3): 233. CrossRef

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IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis: Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho; Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Abstract
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Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

Citations

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Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
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Oriol Jornet‐Rius, Nida Chornarm, Niki Skeldon, Ashley McGrew, Michael Lappin, Laia Solano‐Gallego, A Russell Moore
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Lydia J. Colopy, Kai‐Biu Shiu, Laura A. Snyder, Anne C. Avery, Emily D. Rout, A R. Moore
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Yun Ku Kim, Myung Hwan Kim, Han Seoung Park, Ji Woong Jang, Yil Sik Hyun, Ji Hun Kim
Korean Journal of Medicine.2012; 82(3): 331. CrossRef
Suppression of IP-10/CXCL10 gene expression in LPS- and/or IFN-γ-stimulated macrophages by parasite-secreted products
Soji Fukumoto, Miki Hiroi, Paramasari Dirgahayu, Kazutoyo Miura, Sayuri Tademoto, Hitoshi Otsuki, Yoshihiro Ohmori
Cellular Immunology.2012; 276(1-2): 101. CrossRef
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Jia-Xu Chen, Mu-Xin Chen, Lin Ai, Jun-Hu Chen, Shao-Hong Chen, Yong-Nian Zhang, Yu-Chun Cai, Xing-Quan Zhu, Xiao-Nong Zhou, Patrick J. Lammie
PLoS Neglected Tropical Diseases.2012; 6(11): e1899. CrossRef
Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera
Moizur Rahman, Eung-Goo Lee, Young-An Bae
Parasitology International.2011; 60(2): 139. CrossRef
Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization
Hyun-Jong Yang
The Korean Journal of Parasitology.2010; 48(2): 183. CrossRef
Diagnostic values of IgG4 in human gnathostomiasis
Malinee T. Anantaphruti, S. Nuamtanong, P. Dekumyoy
Tropical Medicine & International Health.2005; 10(10): 1013. CrossRef

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Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA: Woon-Mok Sohn, Ho-Woo Nam; Korean J Parasitol 1999;37(4):249-256.
Published online December 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.4.249

Abstract
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A total of 198 sera from stray cats was assayed against Toxoplasma gondii antigen by western blot. Out of 198 sera assayed, 26 sera (13.1%) showed typical blot patterns against T. gondii. When spotted by ELISA absorbance and indirect latex agglutination test (ILAT) titer, all 26 cases were distributed over the cut-off value of ELISA whereas 24 cases (92.3%) were in the positive range of 1:32 or higher and 2 cases in negative range by ILAT. Among western blot negative 172 sera, 162 cases were negative in both ILAT and ELISA while 10 cases were reactive falsely such that three cases were ILAT positive with 1:32 titer and 9 cases were ELISA positive (2 cases overlapped). These 10 cases reacted peculiarly without typical binding pattern in Western blot. Sandwich-ELISA was performed with monoclonal antibodies (mAbs) of Tg563 (30 kDa, SAG1), Tg505 (22 kDa, SAG2), Tg605 (43 kDa, SAG3), Tg556 (28 kDa, GRA2), Tg737 (32 kDa, GRA6), Tg695 (66 kDa, ROP2), Tg786 (42 kDa, ROP6), and Tg621 (32 kDa, anonymous but cytosolic) clone, respectively. All western blot-positive cases were in the positive range and negative cases in the negative range clearly. Among the 10 false reactive cases, 3 cases were in the positive range with one or more mAbs. All mAbs used in this study were confirmed to be specific to T. gondii infection as a standardized sandwich-ELISA to differentiate it from other pathogens.

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Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA: Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong; Korean J Parasitol 1999;37(3):163-169.
Published online September 20, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.3.163

Abstract
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House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 ?g/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

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Monoclonal antibodies to recombinant Der f 2 and development of a two‐site ELISA sensitive to major Der f 2 isoallergen in Korea
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Variation of antigenicity and serological reaction to Pneumocystis carinii in Korea: Hyun-Young Park, Soo-Ung Lee, Seoung-Wan Chae, Sun Huh, Jae-Ran Yu, Jin Kim, Sung-Tae Hong; Korean J Parasitol 1999;37(2):109-115.
Published online June 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.2.109

Abstract
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The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon, Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-Linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.

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