Parasites, Hosts and Diseases

"Antigens"

Original Articles

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection: Jung-Hwa Cho, Woo-Suk Chung, Kyoung-Ju Song, Byoung-Kuk Na, Seung-Won Kang, Chul-Yong Song, Tong-Soo Kim; Korean J Parasitol 2005;43(1):19-25.
Published online March 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.1.19

Abstract
PDF

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N.caninum. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo.

Citations

Citations to this article as recorded by

Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2
Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello
Royal Society Open Science.2025;[Epub] CrossRef
A Listeria monocytogenes-Based Vaccine Formulation Reduces Vertical Transmission and Leads to Enhanced Pup Survival in a Pregnant Neosporosis Mouse Model
Dennis Imhof, William Robert Pownall, Camille Monney, Anna Oevermann, Andrew Hemphill
Vaccines.2021; 9(12): 1400. CrossRef
Interferon-γ-dependent protection against Neospora caninum infection conferred by mucosal immunization in IL-12/IL-23 p40-deficient mice
Pedro Ferreirinha, Ricardo Fróis-Martins, Luzia Teixeira, António Rocha, Manuel Vilanova, Alexandra Correia
Vaccine.2018; 36(32): 4890. CrossRef
On the application of reverse vaccinology to parasitic diseases: a perspective on feature selection and ranking of vaccine candidates
Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
International Journal for Parasitology.2017; 47(12): 779. CrossRef
Mucosal immunization confers long-term protection against intragastrically established Neospora caninum infection
Pedro Ferreirinha, Alexandra Correia, Maria Teixeira-Coelho, Hugo Osório, Luzia Teixeira, António Rocha, Manuel Vilanova
Vaccine.2016; 34(50): 6250. CrossRef
A 78 kDa host cell invasion protein of Neospora caninum as a potential vaccine candidate
Qiang Lv, Shenyang Xing, Pengtao Gong, Le Chang, Zhengzheng Bian, Lidong Wang, Xichen Zhang, Jianhua Li
Experimental Parasitology.2015; 148: 56. CrossRef
Discovering a vaccine against neosporosis using computers: is it feasible?
Stephen J. Goodswen, Paul J. Kennedy, John T. Ellis
Trends in Parasitology.2014; 30(8): 401. CrossRef
Cloning and expression of Neospora caninum dense-granule 7 in E. coli
Marziye Kefayat, Hossein Hamidinejat, Masoud Reza Seifiabadshapoori, Mohammad Mehdi Namavari, Parviz Shayan, Saad Gooraninejad
Journal of Parasitic Diseases.2014; 38(2): 196. CrossRef
Vaccines against neosporosis: What can we learn from the past studies?
Thierry Monney, Andrew Hemphill
Experimental Parasitology.2014; 140: 52. CrossRef
A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
Yanina P. Hecker, Verónica Cóceres, Silvina E. Wilkowsky, José M. Jaramillo Ortiz, Eleonora L. Morrell, Andrea E. Verna, Agustina Ganuza, Dora B. Cano, Lilian Lischinsky, Sergio O. Ángel, Patricia Zamorano, Anselmo C. Odeón, María R. Leunda, Carlos M. Cam
Veterinary Immunology and Immunopathology.2014; 162(3-4): 142. CrossRef
A guide to in silico vaccine discovery for eukaryotic pathogens
S. J. Goodswen, P. J. Kennedy, J. T. Ellis
Briefings in Bioinformatics.2013; 14(6): 753. CrossRef
ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation
Jun Hiasa, Junko Kohara, Maki Nishimura, Xuenan Xuan, Hiromi Tokimitsu, Yoshifumi Nishikawa
Veterinary Parasitology.2012; 187(3-4): 379. CrossRef
Vaccines against a Major Cause of Abortion in Cattle, Neospora caninum Infection
Thierry Monney, Karim Debache, Andrew Hemphill
Animals.2011; 1(3): 306. CrossRef
Comparative efficacy of immunization with inactivated whole tachyzoites versus a tachyzoite-bradyzoite mixture against neosporosis in mice
S. ROJO-MONTEJO, E. COLLANTES-FERNÁNDEZ, S. RODRÍGUEZ-MARCOS, F. PÉREZ-ZABALLOS, I. LÓPEZ-PÉREZ, A. PRENAFETA, L. M. ORTEGA-MORA
Parasitology.2011; 138(11): 1372. CrossRef
Transgenic Neospora caninum strains constitutively expressing the bradyzoite NcSAG4 protein proved to be safe and conferred significant levels of protection against vertical transmission when used as live vaccines in mice
V. Marugán-Hernández, L.M. Ortega-Mora, A. Aguado-Martínez, E. Jiménez-Ruíz, G. Álvarez-García
Vaccine.2011; 29(44): 7867. CrossRef
Selection of Neospora caninum antigens stimulating bovine CD4+ve T cell responses through immuno-potency screening and proteomic approaches
Mara S Rocchi, Paul M Bartley, Neil F Inglis, Esther Collantes-Fernandez, Gary Entrican, Frank Katzer, Elisabeth A Innes
Veterinary Research.2011; 42(1): 91. CrossRef
RecNcMIC3-1-R is a microneme- and rhoptry-based chimeric antigen that protects against acute neosporosis and limits cerebral parasite load in the mouse model for Neospora caninum infection
Thierry Monney, David Rütti, Michelle Schorer, Karim Debache, Denis Grandgirard, Stephen L. Leib, Andrew Hemphill
Vaccine.2011; 29(40): 6967. CrossRef
Immunization of female BALB/c mice with Neospora cyclophilin and/or NcSRS2 elicits specific antibody response and prevents against challenge infection by Neospora caninum
Wenbin Tuo, Yan Zhao, Daming Zhu, Mark C. Jenkins
Vaccine.2011; 29(13): 2392. CrossRef
Intraperitoneal and intra-nasal vaccination of mice with three distinct recombinantNeospora caninumantigens results in differential effects with regard to protection against experimental challenge withNeospora caninumtachyzoites
K. DEBACHE, C. GUIONAUD, F. ALAEDDINE, A. HEMPHILL
Parasitology.2010; 137(2): 229. CrossRef
Characterisation of NcGRA7 and NcSAG4 proteins: Immunolocalisation and their role in the host cell invasion by Neospora caninum tachyzoites
Adriana Aguado-Mart?nez, Gema ?lvarez-Garc?a, Gereon Schares, Ver?nica Risco-Castillo, Aurora Fern?ndez-Garc?a, Virginia Marug?n-Hern?ndez, Luis Ortega-Mora
Acta Parasitologica.2010;[Epub] CrossRef
CpG-ODN combined with Neospora caninum lysate, but not with excreted-secreted antigen, enhances protection against infection in mice
Dâmaso P. Ribeiro, Marina M.P. Freitas, Mariana R.D. Cardoso, Ana C.A.M. Pajuaba, Neide M. Silva, Tiago W.P. Mineo, João S. Silva, José R. Mineo, Deise A.O. Silva
Vaccine.2009; 27(19): 2570. CrossRef
Characterization of tissue distribution and histopathological lesions in Neospora caninum experimentally infected gerbils
Seung-Won Kang, Sung-Soo Park, Se-Eun Choe, Young-Hwa Jean, Suk-Chan Jung, Keun Kim, Dong Van Quyen
Parasitology Research.2009; 104(6): 1261. CrossRef
Failure of a vaccine using immunogenic recombinant proteins rNcSAG4 and rNcGRA7 against neosporosis in mice
Adriana Aguado-Martínez, Gema Álvarez-García, Aurora Fernández-García, Verónica Risco-Castillo, Virginia Marugán-Hernández, Luis M. Ortega-Mora
Vaccine.2009; 27(52): 7331. CrossRef
Vaccination with recombinant NcROP2 combined with recombinant NcMIC1 and NcMIC3 reduces cerebral infection and vertical transmission in mice experimentally infected with Neospora caninum tachyzoites
Karim Debache, Ferial Alaeddine, Christophe Guionaud, Thierry Monney, Joachim Müller, Maria Strohbusch, Stephen L. Leib, Denis Grandgirard, Andrew Hemphill
International Journal for Parasitology.2009; 39(12): 1373. CrossRef
Neospora caninum – How close are we to development of an efficacious vaccine that prevents abortion in cattle?
Michael P. Reichel, John T. Ellis
International Journal for Parasitology.2009; 39(11): 1173. CrossRef
Immunization with Oligomannose-Coated Liposome-Entrapped Dense Granule Protein 7 Protects Dams and Offspring fromNeospora caninumInfection in Mice
Yoshifumi Nishikawa, Houshuang Zhang, Yuzuru Ikehara, Naoya Kojima, Xuenan Xuan, Naoaki Yokoyama
Clinical and Vaccine Immunology.2009; 16(6): 792. CrossRef
Vaccination of mice with recombinant NcROP2 antigen reduces mortality and cerebral infection in mice infected with Neospora caninum tachyzoites
Karim Debache, Christophe Guionaud, Ferial Alaeddine, Meike Mevissen, Andrew Hemphill
International Journal for Parasitology.2008; 38(12): 1455. CrossRef
Prevention of lethal experimental infection of C57BL/6 mice by vaccination with Brucella abortus strain RB51 expressing Neospora caninum antigens
Sheela Ramamoorthy, Neelima Sanakkayala, Ramesh Vemulapalli, Robert B. Duncan, David S. Lindsay, Gerhart S. Schurig, Stephen M. Boyle, Ramanathan Kasimanickam, Nammalwar Sriranganathan
International Journal for Parasitology.2007; 37(13): 1521. CrossRef
Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins
Ramesh Vemulapalli, Neelima Sanakkayala, Jatinder Gulani, Gerhardt G. Schurig, Stephen M. Boyle, David S. Lindsay, Nammalwar Sriranganathan
Veterinary Parasitology.2007; 148(3-4): 219. CrossRef
VACCINATION WITH MICRONEME PROTEIN NCMIC4 INCREASES MORTALITY IN MICE INOCULATED WITH NEOSPORA CANINUM
Sangeetha Srinivasan, Joachim Mueller, Angela Suana, Andrew Hemphill
Journal of Parasitology.2007; 93(5): 1046. CrossRef
Protecting babies: vaccine strategies to prevent foetopathy inNeospora caninum‐infected cattle
D. J. L. WILLIAMS, A. J. TREES
Parasite Immunology.2006; 28(3): 61. CrossRef
Vaccination as a control strategy against the coccidial parasitesEimeria,ToxoplasmaandNeospora
E. A. INNES, A. N. VERMEULEN
Parasitology.2006; 133(S2): S145. CrossRef
Immunization with native surface protein NcSRS2 induces a Th2 immune response and reduces congenital Neospora caninum transmission in mice
G.J. Haldorson, B.A. Mathison, K. Wenberg, P.A. Conrad, J.P. Dubey, A.J. Trees, I. Yamane, T.V. Baszler
International Journal for Parasitology.2005; 35(13): 1407. CrossRef

8,953 View
94 Download
Crossref

ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas: Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(4):203-207.
Published online December 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.4.203

Abstract
PDF

An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used as template to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1). Each amplified DNA fragment was inserted into pQE30 plasmid to induce the expression of His-tagged protein in Escherichia coli (M15 strain) by IPTG. His-tagged proteins were purified by Ni-NTA metal-affinity chromatography and used as antigens for ELISA with patient sera that were confirmed previously by blood smear examinations. When applied to patient sera, 122 (80.3%) out of 152 vivax malaria cases reacted to at least one antigen, while no reactions were observed with 128 uninfected serum samples. We applied this ELISA to the screening of 3,262 civilian residents in endemic regions near the DMZ, which resulted in 236 positively detected (7.2%) cases. This method can be applied to serological diagnosis and mass screening in endemic regions, or can be used as a safety test for transfusion blood in endemic areas.

Citations

Citations to this article as recorded by

Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Lejla Kartal, Ivo Mueller, Rhea J. Longley
Pathogens.2023; 12(6): 791. CrossRef
Antibodies Against the Plasmodium vivax Apical Membrane Antigen 1 From the Belem Strain Share Common Epitopes Among Other Worldwide Variants
Ana Caroline Barbosa França, Kátia Sanches Françoso, Rodolfo Ferreira Marques, Gustavo H. G. Trossini, Renan A. Gomes, Marinete M. Póvoa, Maristela G. Cunha, Eduardo L. V. Silveira, Irene S. Soares
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Analyses of the expression, immunohistochemical properties and serodiagnostic potential of Schistosoma japonicum peroxiredoxin-4
Minh-Anh Dang-Trinh, Jose Ma. M. Angeles, Kharleezelle J. Moendeg, Adrian Miki C. Macalanda, Thu-Thuy Nguyen, Luna Higuchi, Shotaro Nakagun, Masashi Kirinoki, Yuichi Chigusa, Yasuyuki Goto, Shin-ichiro Kawazu
Parasites & Vectors.2020;[Epub] CrossRef
Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species
Kharleezelle J. Moendeg, Jose Ma. M. Angeles, Yasuyuki Goto, Lydia R. Leonardo, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu
Parasitology Research.2015; 114(3): 1225. CrossRef
Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients
Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim
The Korean Journal of Parasitology.2014; 52(2): 143. CrossRef
Immunological markers of Plasmodium vivaxexposure and immunity: a systematic review and meta-analysis
Julia C Cutts, Rosanna Powell, Paul A Agius, James G Beeson, Julie A Simpson, Freya J I Fowkes
BMC Medicine.2014;[Epub] CrossRef
A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran
Afsaneh Motevalli Haghi, Mohammad Reza Khoramizade, Mehdi Nateghpour, Mehdi Mohebali, Gholam Hossein Edrissian, Mohammad Reza Eshraghian, Zargham Sepehrizadeh
The Korean Journal of Parasitology.2012; 50(1): 15. CrossRef
Isolation and Characterization of the MSP1 Genes from Plasmodium malariae and Plasmodium ovale
George J. Dawson, Suresh M. Desai, Larry Birkenmeyer, A. Scott Muerhoff
The American Journal of Tropical Medicine and Hygiene.2010; 82(6): 996. CrossRef
Detection ofPlasmodium falciparum,P. vivax,P. ovale, andP. malariaeMerozoite Surface Protein 1-p19 Antibodies in Human Malaria Patients and Experimentally Infected Nonhuman Primates
A. Scott Muerhoff, Larry G. Birkenmeyer, Ruthie Coffey, Bruce J. Dille, John W. Barnwell, William E. Collins, Joann S. Sullivan, George J. Dawson, Suresh M. Desai
Clinical and Vaccine Immunology.2010; 17(10): 1631. CrossRef
A new ELISA kit which uses a combination of Plasmodium falciparum extract and recombinant Plasmodium vivax antigens as an alternative to IFAT for detection of malaria antibodies
Cecile Doderer, Aurelie Heschung, Phillippe Guntz, Jean-Pierre Cazenave, Yves Hansmann, Alexandre Senegas, Alexander W Pfaff, Tamer Abdelrahman, Ermanno Candolfi
Malaria Journal.2007;[Epub] CrossRef

8,006 View
81 Download
Crossref

Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda): Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong; Korean J Parasitol 2003;41(3):155-163.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.155

Abstract
PDF

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

Citations

Citations to this article as recorded by

Construction and validation of a novel multi-epitope in silico vaccine design against the paramyosin protein of Opisthorchis viverrini using immunoinformatics analyses
Alok Kafle, Jan Clyden B. Tenorio, Roshan Kumar Mahato, Sahara Dhakal, Muhammad F. Heikal, Sutas Suttiprapa
Acta Tropica.2024; 260: 107389. CrossRef
Antigenic components, identification, and characterization of whole worm extract of Platynosomum illiciens
Shin Moe Aoke, Amaya Watthanadirek, Napassorn Poolsawat, Nitipon Srionrod, Pornpiroon Nooroong, Sutthida Minsakorn, Sitthichok Lacharoje, Woraporn Sukhumavasi, Panat Anuracpreeda
Veterinary Parasitology.2022; 310: 109774. CrossRef
A first attempt at determining the antibody-specific pattern of Platynosomum fastosum crude antigen and identification of immunoreactive proteins for immunodiagnosis of feline platynosomiasis
Babi Kyi Soe, Poom Adisakwattana, Onrapak Reamtong, Panat Anuracpreeda, Woraporn Sukhumavasi
Veterinary World.2022; : 2029. CrossRef
Current Perspectives on Opisthorchiasis Control and Cholangiocarcinoma Detection in Southeast Asia
Narong Khuntikeo, Attapol Titapun, Watcharin Loilome, Puangrat Yongvanit, Bandit Thinkhamrop, Nittaya Chamadol, Thidarat Boonmars, Teerachai Nethanomsak, Ross H. Andrews, Trevor N. Petney, Paiboon Sithithaworn
Frontiers in Medicine.2018;[Epub] CrossRef
Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War
George Psevdos, Florence M. Ford, Sung-Tae Hong
Infectious Diseases in Clinical Practice.2018; 26(4): 208. CrossRef
Comments on “Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War”
Theodore E. Nash, David Sullivan, Edward Mitre, Eric Garges, Victoria J. Davey, Gary Roselle, Stephen J. Davies, Peter D. Rumm
Infectious Diseases in Clinical Practice.2018; 26(4): 240. CrossRef
Responding to Letter “Comments on ‘Screening US Vietnam Veterans for Liver Fluke Exposure 5 Decades After the End of the War’” by Theodore E. Nash, David Sullivan, Edward Mitre, et al
George Psevdos, Florence M. Ford, Sung-Tae Hong
Infectious Diseases in Clinical Practice.2018; 26(4): 242. CrossRef
Characterization of immunogenic Clonorchis sinensis protein fractions by gel filtration chromatography
Duan Pham Ngoc, Yuji Arimatsu, Sasithorn Kaewkes, Banchob Sripa
Asian Pacific Journal of Tropical Disease.2015; 5(4): 284. CrossRef
Eosinophilia in Infectious Diseases
Elise M. O’Connell, Thomas B. Nutman
Immunology and Allergy Clinics of North America.2015; 35(3): 493. CrossRef
Predominance of IL-10 and TGF-β production from the mouse macrophage cell line, RAW264.7, in response to crude antigens from Clonorchis sinensis
Hae Joo Wi, Yan Jin, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Cytokine.2012; 59(2): 237. CrossRef
The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis
Myoung-Ro Lee, Yu-Jung Kim, Dae-Won Kim, Won Gi Yoo, Shin-Hyeong Cho, Kwang Yeon Hwang, Jung-Won Ju, Won-Ja Lee
Molecular and Biochemical Parasitology.2012; 182(1-2): 1. CrossRef
Detection of salivary antibodies to crude antigens of Opisthorchis viverrini in opisthorchiasis and cholangiocarcinoma patients
Ponlatham Chaiyarit, Paiboon Sithithaworn, Chanitra Thuwajit, Puangrat Yongvanit
Clinical Oral Investigations.2011; 15(4): 477. CrossRef
Clonorchis sinensis infection is positively associated with atopy in endemic area
M‐H Choi, Y‐S Chang, M. K. Lim, Y. M. Bae, S‐T Hong, J‐K Oh, E. H. Yun, M‐J Bae, H‐S Kwon, S‐M Lee, H‐W Park, K‐U Min, Y‐Y Kim, S‐H Cho
Clinical & Experimental Allergy.2011; 41(5): 697. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Resistance of cholangiocarcinoma cells to parthenolide-induced apoptosis by the excretory–secretory products of Clonorchis sinensis
Young Ju Kim, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Parasitology Research.2009; 104(5): 1011. CrossRef
Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system
Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi
Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334. CrossRef
Proliferative effects of excretory/secretory products from Clonorchis sinensis on the human epithelial cell line HEK293 via regulation of the transcription factor E2F1
Young Ju Kim, Min-Ho Choi, Sung-Tae Hong, Young Mee Bae
Parasitology Research.2008; 102(3): 411. CrossRef
A Bile Based Study of Clonorchis sinensis Infections in Patients with Biliary Tract Diseases in Ulsan, Korea
Kwang Ro Joo, Sung-Jo Bang
Yonsei Medical Journal.2005; 46(6): 794. CrossRef
Organ-specific antigens of Clonorchis sinensis
Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2004; 42(4): 169. CrossRef
Evaluation of Clonorchis sinensis Recombinant 7-Kilodalton Antigen for Serodiagnosis of Clonorchiasis
Qin-Ping Zhao, Sung-Ung Moon, Hyeong-Woo Lee, Byoung-Kuk Na, Seung-Yull Cho, Yoon Kong, Ming-Sen Jiang, Ai-Hua Li, Tong-Soo Kim
Clinical and Vaccine Immunology.2004; 11(4): 814. CrossRef

9,160 View
107 Download
Crossref

Western blot diagnosis of vivax malaria with multiple stage-specific antigens of the parasite: Eui-Sun Son, Tong Soo Kim, Ho-Woo Nam; Korean J Parasitol 2001;39(2):171-176.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.171

Abstract
PDF

Western blot analysis was performed to diagnose vivax malaria using stage-specific recombinant antigens. Genomic DNA from the whole blood of a malaria patient was used as templates to amplify the coding regions for the antigenic domains of circumsporozoite protein (CSP-1), merozoite surface protein (MSP-1), apical merozoite antigen (AMA-1), serine repeat antigen (SERA), and exported antigen (EXP-1) of Plasmodium vivax. Each amplified DNA fragment was inserted into a pGEX-4T plasmid to induce the expression of GST fusion protein in Escherichia coli by IPTG. The bacterial cell extracts were separated on 10% SDS-PAGE followed by western blot analysis with patient sera which was confirmed by blood smear examination. When applied with patient sera, 147 (91.9%) out of 160 vivax malaria, 12 (92.3%) out of 13 falciparum malaria, and all 9 vivax/falciparum mixed malaria reacted with at least one antigen, while no reactions occurred with 20 normal uninfected sera. In the case of vivax malaria, CSP-1 reacted with 128 (80.0%) sera, MSP-1 with 102 (63.8%), AMA-1 with 128 (80.0%), SERA with 115 (71.9%), and EXP-1 with 89 (55.6%), respectively. We obtained higher detection rates when using 5 antigens (91.9%) rather than using each antigen solely (55.6-80%), a combination of 2 (76.3-87.5%), 3 (85.6-90.6%), or 4 antigens (89.4-91.3%). This method can be applied to serological diagnosis, mass screening in endemic regions, or safety test in transfusion of prevalent vivax malaria.

Citations

Citations to this article as recorded by

A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding
Clinical Infectious Diseases.2022; 74(1): 40. CrossRef
Antigen Discovery in Circulating Extracellular Vesicles From Plasmodium vivax Patients
Iris Aparici-Herraiz, Melisa Gualdrón-López, Carlos J. Castro-Cavadía, Jaime Carmona-Fonseca, María Fernanda Yasnot, Carmen Fernandez-Becerra, Hernando A. del Portillo
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Development of an Immunosensor for PfHRP 2 as a Biomarker for Malaria Detection
Aver Hemben, Jon Ashley, Ibtisam Tothill
Biosensors.2017; 7(3): 28. CrossRef
Expression and Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-3 (MSP-3) for Detection of Human Malaria
Jeremy Ryan De Silva, Yee-Ling Lau, Mun-Yik Fong, Luzia Helena Carvalho
PLOS ONE.2016; 11(7): e0158998. CrossRef
Evaluation of Recombinant Plasmodium knowlesi Merozoite Surface Protein-133 for Detection of Human Malaria
Yee Ling Lau, Fei Wen Cheong, Rohela Mahmud, Mun Yik Fong
The American Journal of Tropical Medicine and Hygiene.2013; 88(5): 835. CrossRef
Identification of Plasmodium vivax Proteins with Potential Role in Invasion Using Sequence Redundancy Reduction and Profile Hidden Markov Models
Daniel Restrepo-Montoya, David Becerra, Juan G. Carvajal-Patiño, Alvaro Mongui, Luis F. Niño, Manuel E. Patarroyo, Manuel A. Patarroyo, Leonardo Mariño-Ramírez
PLoS ONE.2011; 6(10): e25189. CrossRef
ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas
Sera Kim, Hye-Jin Ahn, Tong-Soo Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(4): 203. CrossRef

8,539 View
95 Download
Crossref

IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis: Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho; Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Abstract
PDF

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

Citations

Citations to this article as recorded by

Sparganosis
S. B. Chuelov, A. L. Rossina
CHILDREN INFECTIONS.2024; 23(1): 44. CrossRef
Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay
Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu
Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
Evaluation of serum protein electrophoresis and immunofixation in dogs seropositive for various vector‐borne pathogens
Oriol Jornet‐Rius, Nida Chornarm, Niki Skeldon, Ashley McGrew, Michael Lappin, Laia Solano‐Gallego, A Russell Moore
Veterinary Clinical Pathology.2024; 53(4): 420. CrossRef
Immunoglobulin G4‐related disease in a dog
Lydia J. Colopy, Kai‐Biu Shiu, Laura A. Snyder, Anne C. Avery, Emily D. Rout, A R. Moore
Journal of Veterinary Internal Medicine.2019; 33(6): 2732. CrossRef
A Case of Sparganosis by Ingestion of Undercooked Snakes That Mimicked Peritoneal Carcinomatosis
Yun Ku Kim, Myung Hwan Kim, Han Seoung Park, Ji Woong Jang, Yil Sik Hyun, Ji Hun Kim
Korean Journal of Medicine.2012; 82(3): 331. CrossRef
Suppression of IP-10/CXCL10 gene expression in LPS- and/or IFN-γ-stimulated macrophages by parasite-secreted products
Soji Fukumoto, Miki Hiroi, Paramasari Dirgahayu, Kazutoyo Miura, Sayuri Tademoto, Hitoshi Otsuki, Yoshihiro Ohmori
Cellular Immunology.2012; 276(1-2): 101. CrossRef
A Protein Microarray for the Rapid Screening of Patients Suspected of Infection with Various Food-Borne Helminthiases
Jia-Xu Chen, Mu-Xin Chen, Lin Ai, Jun-Hu Chen, Shao-Hong Chen, Yong-Nian Zhang, Yu-Chun Cai, Xing-Quan Zhu, Xiao-Nong Zhou, Patrick J. Lammie
PLoS Neglected Tropical Diseases.2012; 6(11): e1899. CrossRef
Two-dimensional immunoblot analysis of antigenic proteins of Spirometra plerocercoid recognized by human patient sera
Moizur Rahman, Eung-Goo Lee, Young-An Bae
Parasitology International.2011; 60(2): 139. CrossRef
Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization
Hyun-Jong Yang
The Korean Journal of Parasitology.2010; 48(2): 183. CrossRef
Diagnostic values of IgG4 in human gnathostomiasis
Malinee T. Anantaphruti, S. Nuamtanong, P. Dekumyoy
Tropical Medicine & International Health.2005; 10(10): 1013. CrossRef

7,611 View
87 Download
Crossref

Variation of antigenicity and serological reaction to Pneumocystis carinii in Korea: Hyun-Young Park, Soo-Ung Lee, Seoung-Wan Chae, Sun Huh, Jae-Ran Yu, Jin Kim, Sung-Tae Hong; Korean J Parasitol 1999;37(2):109-115.
Published online June 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.2.109

Abstract
PDF

The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon, Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-Linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.

Citations

Citations to this article as recorded by

Human Immunodeficiency Virus-Infected Patients with PriorPneumocystisPneumonia Exhibit Increased Serologic Reactivity to Several Major Surface Glycoprotein Clones
K. R. Daly, J. V. Koch, N. J. Shire, L. Levin, P. D. Walzer
Clinical and Vaccine Immunology.2006; 13(10): 1071. CrossRef