Parasites, Hosts and Diseases

"Brugia malayi"

Original Article

Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P: Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.645

Abstract
PDF

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

Citations

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Mini Review

Successful Control of Lymphatic Filariasis in the Republic of Korea: Hyeng-Il Cheun, Yoon Kong, Shin-Hyeong Cho, Jong-Soo Lee, Jong-Yil Chai, Joo-Shil Lee, Jong-Koo Lee, Tong-Soo Kim; Korean J Parasitol 2009;47(4):323-335.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.323

Abstract
PDF

A successful experience of lymphatic filariasis control in the Republic of Korea is briefly reviewed. Filariasis in the Republic of Korea was exclusively caused by infection with Brugia malayi. Over the past several decades from the 1950s to 2006, many investigators exerted their efforts to detection, treatment, and follow-up of filariasis patients in endemic areas, and to control filariasis. Mass, combined with selective, treatments with diethylcarbamazine to microfilaria positive persons had been made them free from microfilaremia and contributed to significant decrease of the microfilarial density in previously endemic areas. Significant decrease of microfilaria positive cases in an area influenced eventually to the endemicity of filariasis in the relevant locality. Together with remarkable economic growth followed by improvement of environmental and personal hygiene and living standards, the factors stated above have contributed to blocking the transmission cycle of B. malayi and led to disappearance of this mosquito-borne ancient disease in the Republic of Korea.

Citations

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Elizabeth A Cromwell, Chris A Schmidt, Kevin T Kwong, David M Pigott, Denise Mupfasoni, Gautam Biswas, Shreya Shirude, Elex Hill, Katie M Donkers, Amir Abdoli, Michael R M Abrigo, Victor Adekanmbi, Olatunji O Adetokunboh Sr., Srividya Adinarayanan, Ehsan
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Brief Communication

A survey of Brugia malayi infection on the Heugsan Islands, Korea: Jong-Yil Chai, Soon-Hyung Lee, Sung-Yil Choi, Jong-Soo Lee, Tai-Soon Yong, Kyun-Jong Park, Kyeong-Ae Yang, Keon-Hoon Lee, Mi-Jeng Park, Hyung-Ran Park, Mi-Ja Kim, Han-Jong Rim; Korean J Parasitol 2003;41(1):69-73.
Published online March 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.1.69

Abstract
PDF

Lymphatic filariasis due to Brugia malayi infection was endemic in several areas of South Korea. The infection was controlled, or disappeared, in most areas, with the exception of the remote southwestern islands of Jeonranam-do, including the Heugsan Islands. To discover its current situation, a small-scale survey was performed on the Heugsan Islands in September 2000. A total of 378 people, 151 male and 227 female, living in 8 villages (6 on Daeheugsan-do, 1 on Daejang-do, and 1 on Yeongsan-do) were subjected to a night blood survey for microfilaremia, and physical examination for elephantiasis on the extremities. There were 6 (1.6%) microfilaria positive cases, all in females aged 57-72 years, and from only two villages of the Daeheugsan-do area. There were 4 patients with lower leg elephantiasis, but they showed no microfilaremia. The results show that a low-grade endemicity of filariasis remains on the Daeheugsan-do.

Citations

Citations to this article as recorded by

Prevention and Control Strategies for Parasitic Infections in the Korea Centers for Disease Control and Prevention
Young Yil Bahk, Eun-Hee Shin, Shin-Hyeong Cho, Jung-Won Ju, Jong-Yil Chai, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(5): 401. CrossRef
Follow-up Study of Patients Previously Diagnosed with Lymphatic Filariasis in Korea
Hyeng Il Cheun, Hee Eun Shin, Da Won Ma, Sung Hee Hong, Tae Yun Kim, Sang Eun Lee, JungWon Ju, Yun-Kyu Park, Tong-Soo Kim, Shin Hyeong Cho
Osong Public Health and Research Perspectives.2017; 8(6): 421. CrossRef
Surveillance and Vector Control of Lymphatic Filariasis in the Republic of Korea
Shin Hyeong Cho, Da Won Ma, Bo Ra Koo, Hee Eun Shin, Wook Kyo Lee, Byong Suk Jeong, Chaeshin Chu, Won Ja Lee, Hyeng Il Cheun
Osong Public Health and Research Perspectives.2012; 3(3): 145. CrossRef
Seasonal Prevalence of Mosquitoes, Including Vectors of Brugian Filariasis, in Southern Islands of the Republic of Korea
Hyeng-Il Cheun, Shin-Hyeong Cho, Hee-Il Lee, E-Hyun Shin, Jong-Soo Lee, Tong-Soo Kim, Won-Ja Lee
The Korean Journal of Parasitology.2011; 49(1): 59. CrossRef
Elimination of lymphatic filariasis in the Republic of Korea: an epidemiological survey of formerly endemic areas, 2002–2006
Hyeong‐Il Cheun, Jong‐Soo Lee, Shin‐Hyeong Cho, Yoon Kong, Tong‐Soo Kim
Tropical Medicine & International Health.2009; 14(4): 445. CrossRef
Review of Zoonotic Parasites in Medical and Veterinary Fields in the Republic of Korea
Heejeong Youn
The Korean Journal of Parasitology.2009; 47(Suppl): S133. CrossRef
Successful Control of Lymphatic Filariasis in the Republic of Korea
Hyeng-Il Cheun, Yoon Kong, Shin-Hyeong Cho, Jong-Soo Lee, Jong-Yil Chai, Joo-Shil Lee, Jong-Koo Lee, Tong-Soo Kim
The Korean Journal of Parasitology.2009; 47(4): 323. CrossRef
Trends in parasitic diseases in the Republic of Korea
Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai
Trends in Parasitology.2008; 24(3): 143. CrossRef