Parasites, Hosts and Diseases

"DNA isolation"

Brief Communication

Comparison of Two PCR Assays for Trichomonas vaginalis: Chang-Suk Noh, Sang-Su Kim, Sung-Yul Park, Hong-Sang Moon, Yeonchul Hong, Jae-Sook Ryu; Korean J Parasitol 2019;57(1):27-31.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.27

Abstract
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PCR is known to be the most sensitive method for diagnosing Trichomonas vaginalis infections. This study aimed to compare the sensitivity of a PCR assay for trichomoniasis (HY-PCR) developed in Hanyang University with the use of a Seeplex Ace Detection Kit®, using urine collected from four Korean men with prostatic disease. Overall, HY-PCR was more sensitive than the Seeplex Kit. The use of Chelex 100 is recommended for DNA isolation in order to increase the sensitivity of the PCR test.

Citations

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