Parasites, Hosts and Diseases

"David Blair"

Original Articles

Molecular Variation in the Paragonimus heterotremus Complex in Thailand and Myanmar: Oranuch Sanpool, Pewpan M. Intapan, Tongjit Thanchomnang, Penchom Janwan, Yukifumi Nawa, David Blair, Wanchai Maleewong; Korean J Parasitol 2013;51(6):677-681.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.677

Abstract
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Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. Of the 7 members of the genus known in Thailand until recently, only P. heterotremus has been confirmed as causing human disease. An 8th species, P. pseudoheterotremus, has recently been proposed from Thailand, and has been found in humans. Molecular data place this species as a sister species to P. heterotremus, and it is likely that P. pseudoheterotremus is not specifically distinct from P. heterotremus. In this study, we collected metacercariae of both nominal species (identification based on metacercarial morphology) from freshwater crabs from Phetchabun Province in northern Thailand, Saraburi Province in central Thailand, and Surat Thani Province in southern Thailand. In addition, we purchased freshwater crabs imported from Myanmar at Myawaddy Province, western Thailand, close to the Myanmar-Thailand border. The DNAs extracted from excysted metacercariae were PCR-amplified and sequenced for ITS2 and cox1 genes. The ITS2 sequences were nearly identical among all samples (99-100%). Phylogenies inferred from all available partial cox1 sequences contained several clusters. Sequences from Indian P. heterotremus formed a sister group to sequences from P. pseudoheterotremus-type metacercariae. Sequences of P. heterotremus from Thailand, Vietnam, and China formed a separate distinct clade. One metacercaria from Phitsanulok Province was distinct from all others. There is clearly considerable genetic variation in the P. heterotremus complex in Thailand and the form referred to as P. pseudoheterotremus is widely distributed in Thailand and the Thai-Myanmar border region.

Citations

Citations to this article as recorded by

Considerable genetic diversity within Paragonimus heterotremus in Luang Prabang, northern Lao People's Democratic Republic
Alongkorn Nonthapa, Rutchanee Rodpai, Tongjit Thanchomnang, Patcharaporn Boonroumkaew, Lakkhana Sadaow, David Blair, Pewpan M. Intapan, Wanchai Maleewong, Virasack Banouvong, Sakhone Laymanivong, Oranuch Sanpool
Infection, Genetics and Evolution.2025; 128: 105718. CrossRef
Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny
Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
Parasitology Research.2024;[Epub] CrossRef
Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report
Qiu-Hong Shu, Shu-De Li, Ming Tian, Yong Meng, Shu-Mei-Qi He, Min Zhu, Miao-Miao Wang, Wen-Lin Wang
Acta Parasitologica.2022; 67(1): 316. CrossRef
The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes
Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Urusa Thaenkham
BMC Genomics.2022;[Epub] CrossRef
General overview of the current status of human foodborne trematodiasis
Jong-Yil Chai, Bong-Kwang Jung
Parasitology.2022; 149(10): 1262. CrossRef
Genetic differentiation of Southeast Asian Paragonimus Braun, 1899 (Digenea: Paragonimidae) and genetic variation in the Paragonimus heterotremus complex examined by nuclear DNA sequences
Chairat Tantrawatpan, Sumonta Tapdara, Takeshi Agatsuma, Oranuch Sanpool, Pewpan M. Intapan, Wanchai Maleewong, Weerachai Saijuntha
Infection, Genetics and Evolution.2021; 90: 104761. CrossRef
An outbreak of Paragonimus westermani infection among Cambodian technical intern trainees in Japan, exhibiting various extrapulmonary lesions
Yoshifumi Nakagawa, Yuki Ikematsu, Takayuki Nakanishi, Yuji Ogawa, Ryuichi Taen, Yasunori Nakashima, Hironao Okabe, Ayako Yoshida, Haruhiko Maruyama
Parasitology International.2021; 81: 102279. CrossRef
Development of point-of-care testing tool using immunochromatography for rapid diagnosis of human paragonimiasis
Lakkhana Sadaow, Oranuch Sanpool, Hiroshi Yamasaki, Wanchai Maleewong, Pewpan M. Intapan
Acta Tropica.2020; 203: 105325. CrossRef
Paragonimus and paragonimiasis in Asia: An update
Ayako Yoshida, Pham Ngoc Doanh, Haruhiko Maruyama
Acta Tropica.2019; 199: 105074. CrossRef
Morphological and molecular characterization of Paragonimus caliensis Little, 1968 (Trematoda: Paragonimidae) from Medellin and Pichinde, Colombia
Carolina Lenis, Alicia Galiano, Imelda Vélez, Iván Darío Vélez, Carlos Muskus, Antonio Marcilla
Acta Tropica.2018; 183: 95. CrossRef
Gene diversity and genetic variation in lung flukes (genusParagonimus)
David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh
Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 6. CrossRef
A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region
K. Choudhary, A. Kumar Verma, S. Swaroop, N. Agrawal
Helminthologia.2015; 52(3): 167. CrossRef

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Codon usage and bias in mitochondrial genomes of parasitic platyhelminthes: Thanh Hoa Le, Donald Peter McManus, David Blair; Korean J Parasitol 2004;42(4):159-167.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.159

Abstract
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Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood flukeSchistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.

Citations

Citations to this article as recorded by

Signatures of Endosymbiosis in Mitochondrial Genomes of Rhabdocoel Flatworms
M. Monnens, T. Artois, A. Briscoe, Y. L. Diez, K. P. P. Fraser, B. S. Leander, D. T. J. Littlewood, M. J. Santos, K. Smeets, N. W. L. Van Steenkiste, M. P. M. Vanhove
Molecular Ecology.2025;[Epub] CrossRef
Evolution of codon usage in Taenia saginata genomes and its impact on the host
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Frontiers in Veterinary Science.2023;[Epub] CrossRef
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Experimental Parasitology.2022; 242: 108387. CrossRef
Analysis of codon usage bias of lumpy skin disease virus causing livestock infection
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Frontiers in Veterinary Science.2022;[Epub] CrossRef
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The complete mitochondrial genome of Paragonimus ohirai (Paragonimidae: Trematoda: Platyhelminthes) and its comparison with P. westermani congeners and other trematodes
Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Takeshi Agatsuma, David Blair
PeerJ.2019; 7: e7031. CrossRef
Compositional Analysis of Flatworm Genomes Shows Strong Codon Usage Biases Across All Classes
Guillermo Lamolle, Santiago Fontenla, Gastón Rijo, Jose F. Tort, Pablo Smircich
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Food Science and Biotechnology.2018; 27(3): 725. CrossRef
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Leonie J. Barnett, Terrence L. Miller
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M. Teresa Aguado, Cristina Grande, Michael Gerth, Christoph Bleidorn, Carolina Noreña
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The complete mitochondrial genome of the tapeworm Cladotaenia vulturi (Cestoda: Paruterinidae): gene arrangement and phylogenetic relationships with other cestodes
Aijiang Guo
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Evolutionary Analysis of Mitogenomes from Parasitic and Free-Living Flatworms
Eduard Solà, Marta Álvarez-Presas, Cristina Frías-López, D. Timothy J. Littlewood, Julio Rozas, Marta Riutort, Hector Escriva
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Platyzoan mitochondrial genomes
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