Genetic polymorphisms of pvdhfr and pvdhps genes of Plasmodium vivax were investigated in 83 blood samples collected from patients in the Philippines, Bangladesh, and Nepal. The SNP-haplotypes of the pvdhfr gene at the amino acid positions 13, 33, 57, 58, 61, 117, and 173, and that of the pvdhps gene at the positions 383 and 553 were analyzed by nested PCR-RFLP. Results suggest diverse polymorphic patterns of pvdhfr alone as well as the combination patterns with pvdhps mutant alleles in P. vivax isolates collected from the 3 endemic countries in Asia. All samples carried mutant combination alleles of pvdhfr and pvdhps. The most prevalent combination alleles found in samples from the Philippines and Bangladesh were triple mutant pvdhfr combined with single mutant pvdhps allele and triple mutant pvdhfr combined with double wild-type pvdhps alleles, respectively. Those collected from Nepal were quadruple mutant pvdhfr combined with double wild-type pvdhps alleles. New alternative antifolate drugs which are effective against sulfadoxine-pyrimethamine (SP)-resistant P. vivax are required.
Citations
Citations to this article as recorded by
Molecular surveillance of antifolate drug resistance markers in Plasmodium vivax from Khyber Pakhtunkhwa province, northwest Pakistan Thu Hằng Nguyễn, Hương Giang Lê, Tuấn Cường Võ, Đăng Thùy Dương Nguyễn, Kim Oanh Nguyễn, Minkyoung Cho, Youn-Kyoung Goo, Sahib Gul Afridi, Byoung-Kuk Na Acta Tropica.2025; 264: 107583. CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Geographical spread and structural basis of sulfadoxine-pyrimethamine drug-resistant malaria parasites Rini Chaturvedi, Jyoti Chhibber-Goel, Ishika Verma, Sreehari Gopinathan, Suhel Parvez, Amit Sharma International Journal for Parasitology.2021; 51(7): 505. CrossRef
Molecular monitoring of dihydrofolatereductase (dhfr) and dihydropteroatesynthetase (dhps) associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates of Palawan, Philippines Alison Paolo Bareng, Fe Esperanza Espino, Wanna Chaijaroenkul, Kesara Na-Bangchang Acta Tropica.2018; 180: 81. CrossRef
Mutations of pvdhfr and pvdhps genes in vivax endemic-malaria areas in Kota Marudu and Kalabakan, Sabah Umi Rubiah Sastu, Noor Rain Abdullah, Nor Azrina Norahmad, Muhammad Nor Farhan Saat, Prem Kumar Muniandy, Jenarun Jelip, Moizin Tikuson, Norsalleh Yusof, Hasidah Mohd Sidek Malaria Journal.2016;[Epub] CrossRef
Geographic distribution of amino acid mutations in DHFR and DHPS in Plasmodium vivax isolates from Lao PDR, India and Colombia Naowarat Saralamba, Supatchara Nakeesathit, Mayfong Mayxay, Paul N. Newton, Lyda Osorio, Jung-Ryong Kim, Nicholas J. White, Nicholas P. J. Day, Arjen M. Dondorp, Mallika Imwong Malaria Journal.2016;[Epub] CrossRef
First
Prev
