Parasites, Hosts and Diseases

"Giardia lamblia"

Brief Communication

Prevalence of Intestinal Protozoans among Schoolchildren in Suburban Areas near Yangon, Myanmar: Min-Jae Kim, Bong-Kwang Jung, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Keon-Hoon Lee, Seon Cho, Thi Thi Htoon, Htay Htay Tin, Jong-Yil Chai; Korean J Parasitol 2016;54(3):345-348.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.345

Abstract
PDF

Although intestinal protozoans are common etiologies of diarrhea, few studies have been conducted in Myanmar. This study planned to investigate the prevalence of Giardia lamblia, Entamoeba coli, Entamoeba histolytica, and Endolimax nana among schoolchildren and their guardians in suburban areas near Yangon, Myanmar. We performed a cross-sectional survey among schoolchildren and their guardians from 7 primary schools in South Dagon and Hlaing Thar Yar districts, Yangon, Myanmar. Stool samples were observed with a microscope after concentration technique and iodine staining. Total 821 stool samples, including 556 from schoolchildren and 265 from guardians, were examined. The median age was 6 years old for schoolchildren and 36 years old for guardians. A 53.1% of the school children and 14.6 % of the guardians were males. The overall prevalence of each intestinal protozoan species was as follows: 3.4% (28/821) for G. lamblia; 3.5% (29/821) for E. coli; 1.2% (10/821) for E. histoytica, and 3.0% for E. nana. This study showed that intestinal protozoans are common in primary schoolchildren and their guardians in suburban areas near Yangon, Myanmar. Health interventions, such as hand washing education, improvement of sanitation, and establishment of water purification systems are urgently needed in this area.

Citations

Citations to this article as recorded by

Prevalence and Risk Factors of Giardia intestinalis Infestation and Assemblage of Isolates Among Monastery Primary School Children in Yangon, Myanmar
Yi Yi Myint, Win Pa Pa Aung, Maleewong Wanchai, Pewpan M. Intapan, Oranuch Sanpool, Aung Phyo Wai, Win Win Maw
Cureus.2024;[Epub] CrossRef
Epidemiology and Molecular Characterization of Entamoeba spp. in Non-Human Primates in Zoos in China
Diya An, Shui Yu, Tingting Jiang, Jianhui Zhang, Qun Liu, Jing Liu
Veterinary Sciences.2024; 11(12): 590. CrossRef
Review of zoonotic amebiasis: Epidemiology, clinical signs, diagnosis, treatment, prevention and control
Junqiang Li, Zhaohui Cui, Xiaoying Li, Longxian Zhang
Research in Veterinary Science.2021; 136: 174. CrossRef
Determinants of Pathogen Contamination of the Environment in the Greater Yangon Area, Myanmar
Leon Espira, Ther Aung, Khin Han, Pamela Jagger, Joseph N. S. Eisenberg
Environmental Science & Technology.2021; 55(24): 16465. CrossRef
First identification and genotyping of Enterocytozoon bieneusi in humans in Myanmar
Yujuan Shen, Baiyan Gong, Xiaohua Liu, Yanchen Wu, Fengkun Yang, Jie Xu, Xiaofan Zhang, Jianping Cao, Aiqin Liu
BMC Microbiology.2020;[Epub] CrossRef
The Influence of HIV Status on the Burden and Clinical Manifestations of Gastrointestinal Pathogens in Yangon, Myanmar
Win Pa Pa Aung, Nan Phyu Sin Toe Myint, Thet Tun Zaw, David A. Cooper, Ne Myo Aung, Mar Mar Kyi, Mya Mya Lwin, Josh Hanson
The American Journal of Tropical Medicine and Hygiene.2020; 102(4): 884. CrossRef
Identification of Uncommon Cryptosporidiumviatorum (a Novel Subtype XVcA2G1c) and Cryptosporidium andersoni as Well as Common Giardia duodenalis Assemblages A and B in Humans in Myanmar
Yanchen Wu, Baiyan Gong, Xiaohua Liu, Yanyan Jiang, Jianping Cao, Lan Yao, He Li, Aiqin Liu, Yujuan Shen
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Prevalence and Genetic Identification of Three Entamoeba Species in Pigs in Southeastern China
Ting Ji, Hao-Xuan Cao, Ran Wu, Lin-Lin Cui, Guo-Ming Su, Chang Niu, Ning Zhang, Shou-Kun Wang, Dong-Hui Zhou
BioMed Research International.2019; 2019: 1. CrossRef
Is it necessary to take anthelmintics every year in Korea?
Sun Huh
Journal of the Korean Medical Association.2018; 61(3): 198. CrossRef
Prevalence, molecular epidemiology, and zoonotic potential of Entamoeba spp. in nonhuman primates in China
Haiju Dong, Junqiang Li, Meng Qi, Rongjun Wang, Fuchang Yu, Fuchun Jian, Changshen Ning, Longxian Zhang
Infection, Genetics and Evolution.2017; 54: 216. CrossRef
Intestinal Parasitic Infections and Environmental Water Contamination in a Rural Village of Northern Lao PDR
Alexis Ribas, Chloé Jollivet, Serge Morand, Boupha Thongmalayvong, Silaphet Somphavong, Chern-Chiang Siew, Pei-Jun Ting, Saipin Suputtamongkol, Viengsaene Saensombath, Surapol Sanguankiat, Boon-Huan Tan, Phimpha Paboriboune, Kongsap Akkhavong, Kittipong C
The Korean Journal of Parasitology.2017; 55(5): 523. CrossRef

10,216 View
181 Download
13 Web of Science
Crossref

Original Articles

An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting: Yoursry Hawash, M. M. Ghonaim, S. S. Al-Shehri; Korean J Parasitol 2016;54(1):1-8.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.1

Abstract
PDF

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, RIDA^® Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

Citations

Citations to this article as recorded by

A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates
Ajanta Ghosal, Sanjib K. Sardar, Tapas Haldar, Akash Prasad, Koushik Das, Seiki Kobayashi, Yumiko Saito-Nakano, Shanta Dutta, Tomoyoshi Nozaki, Sandipan Ganguly
Diagnostic Microbiology and Infectious Disease.2024; 110(4): 116548. CrossRef
Comparison of ELISA, nested PCR and sequencing and a novel qPCR for detection of Giardia isolates from Jordan
Nawal Hijjawi, Rongchang Yang, Ma'mon Hatmal, Yasmeen Yassin, Taghrid Mharib, Rami Mukbel, Sameer Alhaj Mahmoud, Abdel-Ellah Al-Shudifat, Una Ryan
Experimental Parasitology.2018; 185: 23. CrossRef
Methods for the detection of Cryptosporidium and Giardia: From microscopy to nucleic acid based tools in clinical and environmental regimes
Folasade Esther Adeyemo, Gulshan Singh, Poovendhree Reddy, Thor Axel Stenström
Acta Tropica.2018; 184: 15. CrossRef
High Frequency of Enteric Protozoan, Viral, and Bacterial Potential Pathogens in Community-Acquired Acute Diarrheal Episodes: Evidence Based on Results of Luminex Gastrointestinal Pathogen Panel Assay
Yousry A. Hawash, Khadiga A. Ismail, Mazen Almehmadi
The Korean Journal of Parasitology.2017; 55(5): 513. CrossRef

11,460 View
167 Download
7 Web of Science
Crossref

Breast-Feeding Protects Infantile Diarrhea Caused by Intestinal Protozoan Infections: Ekhlas Hamed Abdel-Hafeez, Usama Salah Belal, Manal Zaki Mohamed Abdellatif, Koji Naoi, Kazumi Norose; Korean J Parasitol 2013;51(5):519-524.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.519

Abstract
PDF

This study investigated the effect of breast-feeding in protection against protozoan infection in infants with persistent diarrhea. Infants were classified into 2 groups; 161 breast-fed infants and the same number of non-breast-fed infants. Microscopic examinations of stool were done for detection of parasites and measuring the intensity of infection. Moreover, serum levels of IgE and TNF-α were measured by ELISA. Cryptosporidium spp., Entamoeba histolytica/Entamoeba dispar, Giardia lamblia, and Blastocystis sp. were demonstrated in infants with persistent diarrhea. The percentage of protozoan infections was significantly lower in breast-fed infants than that in the non-breast-fed infants. The levels of IgE and TNF-α were significantly lower in the breast-fed group than in the non-breast-fed group. There were significant positive associations between the serum levels of IgE and TNF-α and the intensity of parasite infection in the breast-fed group. It is suggested that breast-feeding has an attenuating effect on the rate and intensity of parasite infection.

Citations

Citations to this article as recorded by

Selected Protective Mechanisms of Human Milk Against Intestinal Protozoal Infections in Infants
Joanna Wróblewska, Anna Długosz, Marcin Wróblewski, Jarosław Nuszkiewicz, Paweł Sutkowy, Alina Woźniak
Current Issues in Molecular Biology.2025; 47(8): 674. CrossRef
Giardia lamblia risk factors and burden in children with acute gastroenteritis in a Nicaraguan birth cohort
Lester Gutiérrez, Nadja A. Vielot, Roberto Herrera, Yaoska Reyes, Christian Toval-Ruíz, Patricia Blandón, Rebecca J. Rubinstein, Javier Mora, Luther A. Bartelt, Filemón Bucardo, Sylvia Becker-Dreps, Samuel Vilchez, Sarman Singh
PLOS Neglected Tropical Diseases.2024; 18(11): e0012230. CrossRef
Tradeoffs in milk immunity affect infant infectious disease risk
Katherine Wander, Masako Fujita, Siobhan M Mattison, Margaret Duris, Megan Gauck, Tessa Hopt, Katherine Lacy, Angela Foligno, Rebecca Ulloa, Connor Dodge, Frida Mowo, Ireen Kiwelu, Blandina T Mmbaga
Evolution, Medicine, and Public Health.2022; 10(1): 295. CrossRef
Intestinal Parasitic Infections Among Pediatric Patients in a Metropolitan City of Bangladesh With Emphasis on Cryptosporidiosis
Nusrat Jahan Nipa, Nasima Aktar, Hasina M Hira, Farhana Akter, Dilshad Jahan, Salequl Islam, Ayukafangha Etando, Adnan Abdullah, Kona Chowdhury, Rahnuma Ahmad, Ahsanul Haq, Mainul Haque
Cureus.2022;[Epub] CrossRef
Association of genetic polymorphism at tumor necrosis factor-α gene promoter - 1031T/C and parasitic infections among children in Northern South Africa
N.C. Davhana, A.K. ElBakri, P.O. Bessong, A. Samie
Heliyon.2020; 6(10): e05129. CrossRef
Antibodies, prophylaxis, transmission
Pierre Lutgen
Pharmacy & Pharmacology International Journal.2018;[Epub] CrossRef
Parasiticidal effect of synthetic bovine lactoferrin peptides on the enteric parasite Giardia intestinalis
Hugo Aguilar-Diaz, Adrian Canizalez-Roman, Tomas Nepomuceno-Mejia, Francisco Gallardo-Vera, Yolanda Hornelas-Orozco, Kamran Nazmi, Jan G.M. Bolscher, Julio Cesar Carrero, Claudia Leon-Sicairos, Nidia Leon-Sicairos
Biochemistry and Cell Biology.2017; 95(1): 82. CrossRef
Cryptosporidium and Giardia in Africa: current and future challenges
Sylvia Afriyie Squire, Una Ryan
Parasites & Vectors.2017;[Epub] CrossRef
Cryptosporidium and Cyclospora Diarrheal Infection in Malnourished Children: a Nutritional Approach
María A. Echevarría, María L. Eva
Current Tropical Medicine Reports.2017; 4(3): 178. CrossRef
Molecular Analysis of the Enteric Protozoa Associated with Acute Diarrhea in Hospitalized Children
Sonia Boughattas, Jerzy M. Behnke, Khalid Al-Ansari, Aarti Sharma, Wafa Abu-Alainin, Asma Al-Thani, Marawan A. Abu-Madi
Frontiers in Cellular and Infection Microbiology.2017;[Epub] CrossRef
Killing of Cryptosporidium sporozoites by Lactoferrin
Jose Luis Paredes, Hayley Sparks, A. Clinton White Jr., Griselle Martinez-Traverso, Theresa Ochoa, Alejandro Castellanos-González
The American Journal of Tropical Medicine and Hygiene.2017; 97(3): 774. CrossRef
Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin-25-Mediated Protection from Amebic Colitis
Zannatun Noor, Koji Watanabe, Mayuresh M. Abhyankar, Stacey L. Burgess, Erica L. Buonomo, Carrie A. Cowardin, William A. Petri, Alan Sher, Ralph Budd, Kris Chadee
mBio.2017;[Epub] CrossRef
Evolution of Socioeconomic Conditions and Its Relation to Spatial–Temporal Changes of Giardiasis and Helminthiasis in Amazonian Children
B. M. Delfino, R. G. Campos, T. M. Pereira, S. A. S. Mantovani, H. Oliart-Guzmán, A. C. Martins, A. M. Braña, F. L. C. C. Branco, J. A. Filgueira-Júnior, A. P. Santos, T. S. Araújo, C. S. M. Oliveira, A. A. Ramalho, P. T. Muniz, C. T. Codeço, M. da Silva-
EcoHealth.2016; 13(4): 743. CrossRef
The Impact of the Milk Glycobiome on the Neonate Gut Microbiota
Alline R. Pacheco, Daniela Barile, Mark A. Underwood, David A. Mills
Annual Review of Animal Biosciences.2015; 3(1): 419. CrossRef
Exclusive breast feeding in early infancy reduces the risk of inpatient admission for diarrhea and suspected pneumonia in rural Vietnam: a prospective cohort study
Sarah Hanieh, Tran T. Ha, Julie A. Simpson, Tran T. Thuy, Nguyen C. Khuong, Dang D. Thoang, Thach D. Tran, Tran Tuan, Jane Fisher, Beverley-Ann Biggs
BMC Public Health.2015;[Epub] CrossRef
Noninvasive intestinal amebiasis
Gayatri V. Nair, Easwaran P. Variyam
Current Opinion in Infectious Diseases.2014; 27(5): 465. CrossRef
Breastfeeding and risk of parasitic infection-a review
Prameela Kannan Kutty
Asian Pacific Journal of Tropical Biomedicine.2014; 4(11): 847. CrossRef

13,613 View
104 Download
Crossref

Brief Communications

Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification: Jie Li, Peiyuan Wang, Aiguo Zhang, Ping Zhang, Muhamd Alsarakibi, Guoqing Li; Korean J Parasitol 2013;51(2):237-241.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.237

Abstract
PDF

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of G. lamblia from dogs. The fecal samples were collected and prepared for microscopic analysis, and then the genomic DNA was extracted directly from purified cysts. The concentration of DNA samples of G. lamblia were diluted by 10-fold serially ranging from 10^-1 to 10^-5 ng/?l for LAMP and PCR assays. The LAMP assay allows the amplification to be finished within 60 min under isothermal conditions of 63℃ by employing 6 oligonucleotide primers designed based on G. lamblia elongation factor 1 alpha (EF1α) gene sequence. Our tests showed that the specific amplification products were obtained only with G. lamblia, while no amplification products were detected with DNA of other related protozoans. Sensitivity evaluation indicated that the LAMP assay was sensitive 10 times more than PCR. It is concluded that LAMP is a rapid, highly sensitive and specific DNA amplification technique for detection of G. lamblia, which has implications for effective control and prevention of giardiasis.

Citations

Citations to this article as recorded by

Evaluation of loop mediated isothermal amplification, quantitative real-time PCR, conventional PCR methods for identifying Ascaris lumbricoides in human stool samples
Mamta Thakur, Abhishek Mewara, PVM Lakshmi, Sucheta Guleria, Sumeeta Khurana
Diagnostic Microbiology and Infectious Disease.2025; 112(3): 116808. CrossRef
Evaluation of Touchdown Loop-Mediated Isothermal Amplification for the Detection of Giardia duodenalis
Márton Hartdégen, András József Laki, Kolos Farkasvölgyi, Kristóf Iván, Judit Plutzer
Parasitologia.2025; 5(2): 25. CrossRef
Real‐time loop‐mediated isothermal amplification (real‐time LAMP) assay for rapid diagnosis of amoebic liver abscess
Sandhya Khunger, Abhishek Mewara, Upninder Kaur, Ajay Duseja, Pallab Ray, Naveen Kalra, Navneet Sharma, Rakesh Sehgal
Tropical Medicine & International Health.2024; 29(2): 104. CrossRef
A colorimetric assay for Neospora caninum utilizing the loop-mediated isothermal amplification technique
Tingting Liu, Kairao Hu, Meiyi Chen, Hongrong Hong, Xi Jiang, Rongsheng Huang, Yiwen Wang, Jing Huang, Xingang Yu, Quan Liu, Zhengkai Wei
Research in Veterinary Science.2024; 179: 105395. CrossRef
Advances in point-of-care and molecular techniques to detect waterborne pathogens
Meysam Khodaparast, Dave Sharley, Stephen Marshall, Travis Beddoe
npj Clean Water.2024;[Epub] CrossRef
Detection of chronic toxoplasmosis in the brain of mice using loop-mediated isothermal amplification (LAMP) and conventional PCR
Mona K. Hegazy, Nora E. Saleh, Wafaa A. Aboukamar
Experimental Parasitology.2023; 251: 108556. CrossRef
A rapid multiplex loop-mediated isothermal amplification (mLAMP) assay for detection of Entamoeba histolytica and Giardia duodenalis
Abhishek Mewara, Sandhya Khunger, Chayan Sharma, Sivanantham Krishnamoorthi, Shreya Singh, Rakesh Yadav, Sumeeta Khurana, Rakesh Sehgal
Letters in Applied Microbiology.2023;[Epub] CrossRef
Disease Biomarkers of Giardiasis
Norhamizah Roshidi, Norsyahida Arifin, Francisco Gonzalez Salazar
Journal of Parasitology Research.2022; 2022: 1. CrossRef
Optimization and validation of a loop-mediated isothermal amplification (LAMP) assay for detection of Giardia duodenalis in leafy greens
Laura F. Lalonde, Vincent Xie, Jenna R. Oakley, Vladislav A. Lobanov
Food and Waterborne Parasitology.2021; : e00123. CrossRef
Loop mediated isothermal amplification (LAMP) of Toxoplasma DNA from dried blood spots
Mona K. Hegazy, Soha I. Awad, Nora E. Saleh, Mamdouh M. Hegazy
Experimental Parasitology.2020; 211: 107869. CrossRef
Sensitive and rapid detection of Ortleppascaris sinensis (Nematoda: Ascaridoidea) by loop-mediated isothermal amplification
Jinhong Zhao, Wei Xu, Genjun Tu, Yongkang Zhou, Xiaobing Wu
PeerJ.2019; 7: e7607. CrossRef
Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis
Aongart Mahittikorn, Nipa Thammasonthijarern, Amonrattana Roobthaisong, Ruenruetai Udonsom, Supaluk Popruk, Sukhontha Siri, Hirotake Mori, Yaowalark Sukthana
Parasites & Vectors.2017;[Epub] CrossRef
Development of a Loop Mediated Isothermal Amplification for Diagnosis ofAscaris lumbricoidesin Fecal Samples
Esther A. Shiraho, Agola L. Eric, Ibrahim N. Mwangi, Geoffrey M. Maina, Joseph M. Kinuthia, Martin W. Mutuku, Robert M. Mugambi, Jackson M. Mwandi, Gerald M. Mkoji
Journal of Parasitology Research.2016; 2016: 1. CrossRef
Molecular Detection of <i>Giardia intestinalis</i> from Stray Dogs in Animal Shelters of Gyeongsangbuk-do (Province) and Daejeon, Korea
Jin-Cheol Shin, Alisha Wehdnesday Bernardo Reyes, Sang-Hun Kim, Suk Kim, Hyung-Jin Park, Kyoung-Won Seo, Kun-Ho Song
The Korean Journal of Parasitology.2015; 53(4): 477. CrossRef
Loop-mediated isothermal amplification assay for detection ofHistomonas meleagridisinfection in chickens targeting the 18S rRNA sequences
Jinjun Xu, Chanbao Qu, Jianping Tao
Avian Pathology.2014; 43(1): 62. CrossRef
Diagnostic testing for Giardia infections
M. F. Heyworth
Transactions of the Royal Society of Tropical Medicine and Hygiene.2014; 108(3): 123. CrossRef

10,465 View
100 Download
Crossref

A Survey of Intestinal Protozoan Infections among Gastroenteritis Patients during a 3-Year Period (2004-2006) in Gyeonggi-do (Province), South Korea: Jeong-Weon Huh, Su-Gyeong Moon, Young-Hee Lim; Korean J Parasitol 2009;47(3):303-305.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.303

Abstract
PDF

The incidence and etiology of parasite-associated gastroenteritis during 2004-2006 in Gyeonggi-do (province), South Korea was determined by means of antigen detection ELISA on 6,071 stool specimens collected from 6 general hospitals. At least 1 parasitic agent was detected in 3.4% (208/6,071) of the stool samples. Among these, Giardia lamblia was the most numerous (152 cases; 2.5%), followed by Entamoeba histolytica (25 cases; 0.4%), Cryptosporidium parvum (23 cases; 0.4%), and mixed infections (8 cases; 0.1%). Patients aged 1-5 years had the largest proportion (69.2%; 144/208) of parasite-positive stool specimens. Parasite-mediated gastroenteritis was most common from June to September. The detection rate gradually increased from 2004 to 2006. This study shows that parasite-mediated gastroenteritis may be significant among children in Korea and that parasite infection surveillance should be constantly performed.

Citations

Citations to this article as recorded by

Gut protozoa of wild rodents – a meta-analysis
Simon Hunter-Barnett, Mark Viney
Parasitology.2024; 151(6): 594. CrossRef
Epidemiology of human giardiasis in Romania: A 14 years survey
Adriana Codrean, Dan L. Dumitrascu, Victor Codrean, Delia Mirela Tit, Simona Bungau, Selim Aleya, Marius Rus, Ovidiu Fratila, Delia Carmen Nistor-Cseppento, Lotfi Aleya, Nicoleta Negrut
Science of The Total Environment.2020; 705: 135784. CrossRef
Multilocus genotyping of Giardia duodenalis from pigs in Korea
Haeseung Lee, ByeongYeal Jung, Jun-Sik Lim, Min-Goo Seo, Seung-Hun Lee, Kwang-Ho Choi, Mi-Hye Hwang, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak
Parasitology International.2020; 78: 102154. CrossRef
Review of Successful Control of Parasitic Infections in Korea
Sung-Tae Hong, Tai-Soon Yong
Infection & Chemotherapy.2020; 52(3): 427. CrossRef
Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
The Korean Journal of Parasitology.2019; 57(5): 531. CrossRef
Etiological Agents of Pediatric Diarrhea in Ardebil, Northwestern Iran
Leila Asadi, Tala Pourlak, Behrooz Ahmadi, Mina Aghamali, Mohammad Asgharzadeh, Mohammad Aghazadeh, Elham Zeinalzadeh, Hossein Samadi Kafil
Archives of Pediatric Infectious Diseases.2018;[Epub] CrossRef
Monitoring of Noxious Protozoa for Management of Natural Water Resources
Young Yil Bahk, Pyo Yun Cho, Sung Kyu Ahn, Sangjung Park, Won Hwa Jheong, Yun-Kyu Park, Ho-Joon Shin, Sang-Seob Lee, Okjae Rhee, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(2): 205. CrossRef
Molecular epidemiology and multilocus sequence analysis of potentially zoonotic Giardia spp. from humans and dogs in Jamaica
Mellesia F. Lee, Paul Cadogan, Sarah Eytle, Sonia Copeland, Julia Walochnik, John F. Lindo
Parasitology Research.2017; 116(1): 409. CrossRef
Detection of Intestinal Parasites in Diarrhea Samples Using Various Diagnostic Methods and Evaluation of the Stability of In-house Quality Control Materials for Stool Examination
Eun Jeong Won, Ji Seung Jung, Jun Hyung Lee, Hyun Jung Choi, Seung Jung Kee, Soo Hyun Kim, Myung Geun Shin, Jong Hee Shin, Soon Pal Suh
Journal of Laboratory Medicine and Quality Assurance.2017; 39(2): 90. CrossRef
Detection and Molecular Characterization of <i>Cryptosporidium</i> spp. from Wild Rodents and Insectivores in South Korea
Juha Song, C-Yoon Kim, Seo-Na Chang, Tamer Said Abdelkader, Juhee Han, Tae-Hyun Kim, Hanseul Oh, Ji Min Lee, Dong-Su Kim, Jong-Taek Kim, Hong-Shik Oh, Moonsuk Hur, Jae-Hwa Suh, Jae-Hak Park
The Korean Journal of Parasitology.2015; 53(6): 737. CrossRef
A Survey of Intestinal Parasite Infection during a 10-Year Period (2003-2012)
Young-Eun Kim, Hee Jae Huh, Yu-Yean Hwang, Nam Yong Lee
Annals of Clinical Microbiology.2013; 16(3): 134. CrossRef
Health Risk Assessment of Cryptosporidium in Tap Water in Korea
Mok-Young Lee, Sang-Jung Park, Eun-Joo Cho, Su-Jeong Park, Sun-Hee Han, Oh-Sang Kwon
Korean Journal of Environmental Health Sciences.2013; 39(1): 32. CrossRef
Enteric Protozoa in the Developed World: a Public Health Perspective
Stephanie M. Fletcher, Damien Stark, John Harkness, John Ellis
Clinical Microbiology Reviews.2012; 25(3): 420. CrossRef
A Ten-year Survey ofGiardia Cystsin Drinking Water Supplies of Seoul, the Republic of Korea
Mok-Young Lee, Eun-Joo Cho, Jin-Hyo Lee, Sun-Hee Han, Yong-Sang Park
The Korean Journal of Parasitology.2011; 49(1): 9. CrossRef
Zoonotic Potential and Molecular Epidemiology ofGiardiaSpecies and Giardiasis
Yaoyu Feng, Lihua Xiao
Clinical Microbiology Reviews.2011; 24(1): 110. CrossRef

9,355 View
126 Download
Crossref

Evaluation of α-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia: Juri Kim, Myeong Heon Shin, Kyoung-Ju Song, Soon-Jung Park; Korean J Parasitol 2009;47(3):287-291.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.287

Abstract
PDF

The α/β-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant α-tubulin of Giardia lamblia were made, and found effective as a probe to specifically detect G. lamblia by immunofluorescence assays. Nucleotide sequences of α-tubulin genes were compared between G. lamblia WB and GS strains, prototypes of assemblage A and assemblage B, respectively. A set of primers was designed and used to amplify a portion of the α-tubulin gene from G. lamblia. PCR-RFLP analysis of this α-tubulin PCR product successfully differentiated G. lamblia into 2 distinct groups, assemblages A and B. The results indicate that α-tubulin can be used as a molecular probe to detect G. lamblia.

Citations

Citations to this article as recorded by

Genetic diversity and molecular diagnosis of Giardia
Yankai Chang, Junqiang Li, Longxian Zhang
Infection, Genetics and Evolution.2023; 113: 105482. CrossRef
Tubulin as a potential molecular target for resveratrol in Giardia lamblia trophozoites, in vitro and in silico approaches
José Roberto Vargas-Villanueva, Filiberto Gutiérrez-Gutiérrez, Mariana Garza-Ontiveros, Sendar Daniel Nery-Flores, Lizeth Guadalupe Campos-Múzquiz, Dagoberto Vazquez-Obregón, Raul Rodriguez-Herrera, Lissethe Palomo-Ligas
Acta Tropica.2023; 248: 107026. CrossRef
Phosphorylation of Serine 148 in Giardia lamblia End‐binding 1 Protein is Important for Cell Division
Juri Kim, Hye‐Yeon Lee, Kyu‐Ho Lee, Soon‐Jung Park
Journal of Eukaryotic Microbiology.2017; 64(4): 464. CrossRef
Host defences against Giardia lamblia
G. Lopez‐Romero, J. Quintero, H. Astiazarán‐García, C. Velazquez
Parasite Immunology.2015; 37(8): 394. CrossRef
Characterization of Microtubule-Binding and Dimerization Activity of Giardia lamblia End-Binding 1 Protein
Juri Kim, Sara Nagami, Kyu-Ho Lee, Soon-Jung Park, Ira J. Blader
PLoS ONE.2014; 9(5): e97850. CrossRef
Zoonotic potential of Giardia
Una Ryan, Simone M. Cacciò
International Journal for Parasitology.2013; 43(12-13): 943. CrossRef
Identification of Antigenic Proteins in Trichomonas vaginalis
Hye-Yeon Lee, Sujin Hyung, Jong Woong Lee, Juri Kim, Myeong Heon Shin, Jae-Sook Ryu, Soon-Jung Park
The Korean Journal of Parasitology.2011; 49(1): 79. CrossRef

8,385 View
115 Download
Crossref

Intestinal protozoan infections and echinococcosis in the inhabitants of Dornod and Selenge, Mongolia (2003): Sun Huh, Jae-Ran Yu, Jong-Il Kim, Choijamts Gotov, Radnaabazar Janchiv, Jeong-Sun Seo; Korean J Parasitol 2006;44(2):171-174.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.171

Abstract
PDF

The status of intestinal parasitic infections and seroprevalence of cystic echinococcosis in the inhabitants in Dornod and Selenge, Mongolia, was observed with stool and serum samples from 165 and 683 residents from August 9 to August 15, 2003. A total of 10 inhabitants (6.1%) were found to be infected with protozoan cysts or oocysts by stool examinations; 7, 1 and 2 cases with Entamoeba coli, Giardia lamblia and Cryptosporidium parvum, respectively. A total of 62 people (9.1%) revealed significantly high levels of specific antibodies against Echinococcus granulosus by enzyme-linked immunosorbent assay (ELISA). The surveyed areas are rural areas and the sanitized tab water is not available. There is a possibility of endemic transmission of water-borne protozoan diseases in these areas. The clinical echinococcosis is needed to be searched from the seropositive inhabitants.

Citations

Citations to this article as recorded by

Prevalence of intestinal protozoan parasites among Asian schoolchildren: a systematic review and meta-analysis
Amir Abdoli, Meysam Olfatifar, Aida Vafae Eslahi, Zeinab Moghadamizad, Oskar Nowak, Majid Pirestani, Amir Karimipour-saryazdi, Milad Badri, Panagiotis Karanis
Infection.2024; 52(6): 2097. CrossRef
Prevalence and risk factors associated with human cystic echinococcosis in rural areas, Mongolia
Temuulen Dorjsuren, Sumiya Ganzorig, Munkhbaatar Dagvasumberel, Altansukh Tsend-Ayush, Chimedlkhamsuren Ganbold, Mandukhai Ganbat, Enkh-Oyun Tsogzolbaatar, Uranchimeg Tsevelvaanchig, Giimaa Narantsogt, Chinchuluun Boldbaatar, Burnee Mundur, Munkhgerel Kha
PLOS ONE.2020; 15(7): e0235399. CrossRef
Patients with cystic echinococcosis in the three national referral centers of Mongolia: A model for CE management assessment
Bolor Bold, Jan Hattendorf, Agiimaa Shagj, Bayar Tserendovdon, Tsendjav Ayushkhuu, Amgalan Luvsandorj, Jakob Zinsstag, Thomas Junghanss, Francesca Tamarozzi
PLOS Neglected Tropical Diseases.2018; 12(8): e0006686. CrossRef
The echinococcoses in Asia: The present situation
Akira Ito, Christine M. Budke
Acta Tropica.2017; 176: 11. CrossRef
Epidemiology and control of echinococcosis in central Asia, with particular reference to the People's Republic of China
Wenbao Zhang, Zhuangzhi Zhang, Weiping Wu, Baoxin Shi, Jun Li, Xiaonong Zhou, Hao Wen, Donald P. McManus
Acta Tropica.2015; 141: 235. CrossRef
The present situation of echinococcoses in Mongolia
A. Ito, C.M. Budke
Journal of Helminthology.2015; 89(6): 680. CrossRef
Cystic Echinococcoses in Mongolia: Molecular Identification, Serology and Risk Factors
Akira Ito, Temuulen Dorjsuren, Anu Davaasuren, Tetsuya Yanagida, Yasuhito Sako, Kazuhiro Nakaya, Minoru Nakao, Oyun-Erdene Bat-Ochir, Tsendjav Ayushkhuu, Narantuya Bazarragchaa, Nyamkhuu Gonchigsengee, Tiaoying Li, Gurbadam Agvaandaram, Abmed Davaajav, Ch
PLoS Neglected Tropical Diseases.2014; 8(6): e2937. CrossRef

7,954 View
95 Download
Crossref

Original Article

In vivo determination of the gap2 gene promoter activity in Giardia lamblia: Hye-Won Yang, Juri Kim, Tai-Soon Yong, Soon-Jung Park; Korean J Parasitol 2006;44(1):21-26.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.21

Abstract
PDF

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

Citations

Citations to this article as recorded by

Eukaryote-conserved histone post-translational modification landscape in Giardia duodenalis revealed by mass spectrometry
Samantha J. Emery-Corbin, Joshua J. Hamey, Balu Balan, Laura Rojas-López, Staffan G. Svärd, Aaron R. Jex
International Journal for Parasitology.2021; 51(4): 225. CrossRef
Trans-spliced Heat Shock Protein 90 Modulates Encystation in Giardia lamblia
Rishi Kumar Nageshan, Nainita Roy, Shatakshi Ranade, Utpal Tatu, Rhoel Ramos Dinglasan
PLoS Neglected Tropical Diseases.2014; 8(5): e2829. CrossRef