Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong
Korean J Parasitol 2018;56(5):437-446. Published online October 31, 2018
To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
Citations
Citations to this article as recorded by
Molecular survey of Toxoplasma gondii B1 gene in pigs from various localities in Korea Dongmi Kwak, Min-Goo Seo Parasites, Hosts and Diseases.2024; 62(3): 294. CrossRef
Foodborne transmission of Toxoplasma gondii infection in the last decade. An overview S. Almeria, J.P. Dubey Research in Veterinary Science.2021; 135: 371. CrossRef
Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review K. L. D. Tharaka D. Liyanage, Anke Wiethoelter, Jasmin Hufschmid, Abdul Jabbar Pathogens.2021; 10(5): 605. CrossRef
All about Toxoplasma gondii infections in pigs: 2009–2020 Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong Yang, Chunlei Su Veterinary Parasitology.2020; 288: 109185. CrossRef
WITHDRAWN: All about Toxoplasma gondii infections in pigs: the past decade Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong R. Yang, Chunlei Su Veterinary Parasitology.2020; : 109182. CrossRef
Zoonotic parasites are animal parasites that can infect humans. The major zoonotic protozoa in the Republic of Korea are Babesia bovis, Chilomastix mesnili, Cryptosporidium parvum, Endolimax nana, Entamoeba coli, Entamoeba hitolytica, Giardia lamblia, Iodamoeba b?tschlii, Pneumocystis carinii, Sarcocystis cruzi, and Toxoplasma gondii. The major zoonotic helminths in Korea include trematodes, cestodes, and nematodes. Trematodes are Clonorchis sinensis, Echinostoma hortense, Echinostoma spp., Fasciola hepatica, Heterophyes nocens, Metagonimus yokogawai, and Paragonimus westermani. Cestodes are Diphyllobothrium latum, Dipylidium caninum, Echinococcus granulosus, Hymenolepis nana, Raillietina tetragona, sparganum (Spirometra spp.), Taenia saginata, T. solium, and T. asiatica. Nematodes are Ancylostoma caninum, Brugia malayi, Capillaria hepatica, Dirofilaria immitis, Gnathostoma dololesi, Gnathostoma spinigerum, Loa loa, Onchocerca gibsoni, Strongyloides stercoralis, Thelazia callipaeda, Trichinella spiralis, Trichostrongylus orientalis, Trichuris trichiura, and Trichuris vulpis. The one arthropod is Sarcoptes scabiei. Many of these parasites have disappeared or were in decline after the 1990's. Since the late 1990's, the important zoonotic protozoa have been C. parvum, E. nana, E. coli, E. hitolytica, G. lamblia, I. buetschlii, P. carinii and T. gondii. The important zoonotic helminths have been C. sinensis, H. nocens, M. yokogawai, P. westermani, D. latum, T. asiatica, sparganum, B. malayi, T. orientalis, T. callipaeda and T. spiralis. However, outbreaks of these parasites are only in a few endemic areas. The outbreaks of Enterobius vermicularis and head lice, human parasites, have recently increased in the kindergartens and primary schools in the Republic of Korea.
Citations
Citations to this article as recorded by
Prevalence of parasitic infections in stray cats from Gimpo-si, Gyeonggi-do, Korea Sooji Hong, Hyejoo Shin, Seungwan Ryoo, Chung-Won Lee, Jae-Young Park, Jong-Yil Chai, Bong-Kwang Jung Parasites, Hosts and Diseases.2025; 63(2): 182. CrossRef
Prevalence of gastrointestinal parasitic infections in wild mammals of a safari park and a zoo in Bangladesh Sabrina Ferdous, Joya Chowdhury, Tanjila Hasan, Pronesh Dutta, Md. Mizanur Rahman, Mohammad Mahmudul Hassan, Md. Rayhan Faruque, Mohammad Abdul Alim Veterinary Medicine and Science.2023; 9(3): 1385. CrossRef
Perceptions, attitudes and practices regarding canine zoonotic helminthiases among dog owners in Nyagatare district, Rwanda Pie Ntampaka, François Niragire, Vincent Nkurunziza, Gisele Uwizeyimana, Anselme Shyaka Veterinary Medicine and Science.2022; 8(4): 1378. CrossRef
Seropositivity Rates of Strongyloides stercoralis Antibody in the Southeastern Region of Republic of Korea: A Single-Center Retrospective Study Taehwa Kim, Seungjin Lim The Korean Journal of Parasitology.2022; 60(3): 181. CrossRef
Evaluation of zoonotic platyhelminthe infections identified in slaughtered livestock in Iran, 2015–2019 Behzad Kiani, Christine M. Budke, Ebrahim Shams Abadi, Soheil Hashtarkhani, Amene Raouf Rahmati, Mostafa AkbarPour, Mehdi Zarean, Bibi Razieh Hosseini Farash, Fatemeh Kiani, Elham Moghaddas BMC Veterinary Research.2021;[Epub] CrossRef
Parasites Circulating in Wild Synanthropic Capybaras (Hydrochoerus hydrochaeris): A One Health Approach Manuel Uribe, Carlos Hermosilla, Arlex Rodríguez-Durán, Juan Vélez, Sara López-Osorio, Jenny J. Chaparro-Gutiérrez, Jesús A. Cortés-Vecino Pathogens.2021; 10(9): 1152. CrossRef
Gastrointestinal parasites in the opossum Didelphis aurita: Are they a potential threat to human health? Marcos Antônio Bezerra-Santos, Carolina Silveira Fontes, Bárbara Cristina Félix Nogueira, Ricardo Seiti Yamatogi, Rafael Antonio Nascimento Ramos, Juliana Arena Galhardo, Luis Fernando Viana Furtado, Élida Mara Leite Rabelo, Jackson Victor de Araújo, Artu Journal of Parasitic Diseases.2020; 44(2): 355. CrossRef
Helminth Parasites among Rodents in the Middle East Countries: A Systematic Review and Meta-Analysis Md Mazharul Islam, Elmoubashar Farag, Mohammad Mahmudul Hassan, Devendra Bansal, Salah Al Awaidy, Abdinasir Abubakar, Hamad Al-Romaihi, Zilungile Mkhize-Kwitshana Animals.2020; 10(12): 2342. CrossRef
Monitoring of Cysticercosis of rabbits in farms of different forms of ownership M. V. Bogach, O. O. Horobei, O. M. Ivanchenko, D. V. Vovk Journal for Veterinary Medicine, Biotechnology and Biosafety.2020; 6(3): 9. CrossRef
Anaerobic digestion of agricultural manure and biomass – Critical indicators of risk and knowledge gaps Rajat Nag, Agathe Auer, Bryan K. Markey, Paul Whyte, Stephen Nolan, Vincent O'Flaherty, Lauren Russell, Declan Bolton, Owen Fenton, Karl Richards, Enda Cummins Science of The Total Environment.2019; 690: 460. CrossRef
IDENTIFICATION AND PREVALENCE OF GASTROINTESTINAL PARASITES IN BEEF CATTLE IN SIAK SRI INDRAPURA, RIAU, INDONESIA Lucia Tri Suwanti Indonesian Journal of Tropical and Infectious Disease.2019; 7(6): 155. CrossRef
Comparative proteomics analysis of Trichinella spiralis muscle larvae exposed to albendazole sulfoxide stress Ruo Yu Peng, Hui Jun Ren, Chun Li Zhang, Pin Lv, Gao Hui Wei, Liang Ming Acta Tropica.2018; 185: 183. CrossRef
Zoonotic intestinal parasites of carnivores: A systematic review in Iran Shahabeddin Sarvi, Ahmad Daryani, Mehdi Sharif, Mohammad Taghi Rahimi, Mohammad Hasan Kohansal, Siavash Mirshafiee, Abolghasem Siyadatpanah, Seyed-Abdollah Hosseini, Shirzad Gholami Veterinary World.2018; 11(1): 58. CrossRef
Serological and Molecular Detection of Toxoplasma gondii and Babesia microti in the Blood of Rescued Wild Animals in Gangwon-do (Province), Korea Sung-Hee Hong, Hee-Jong Kim, Young-Il Jeong, Shin-Hyeong Cho, Won-Ja Lee, Jong-Tak Kim, Sang-Eun Lee The Korean Journal of Parasitology.2017; 55(2): 207. CrossRef
Characteristic CT and MR imaging findings of cerebral paragonimiasis Yong Xia, Jing Chen, Yan Ju, Chao You Journal of Neuroradiology.2016; 43(3): 200. CrossRef
Human Intestinal Parasites From the Wushantou Site in Neolithic Period Taiwan (800–1 BC) Hui-Yuan Yeh, Yu-Pei Chen, Piers D. Mitchell The Journal of Island and Coastal Archaeology.2016; 11(3): 425. CrossRef
Microbiological survey of mice (Mus musculus) purchased from commercial pet shops in Kanagawa and Tokyo, Japan Nobuhito HAYASHIMOTO, Hanako MORITA, Tomoko ISHIDA, Ritsuki UCHIDA, Mai TANAKA, Midori OZAWA, Masahiko YASUDA, Toshio ITOH Experimental Animals.2015; 64(2): 155. CrossRef
Trichinellosis: Characteristics of the cause, mode of transmission and the possibility of prevention Jelena Antić-Stanković Hrana i ishrana.2015; 56(2): 12. CrossRef
A slaughterhouse study on prevalence of some helminths of cattle in Lorestan provience, west Iran Behrouz Ezatpour, Ali Hasanvand, Mehdi Azami, Hossein Mahmoudvand, Khatereh Anbari Asian Pacific Journal of Tropical Disease.2014; 4(5): 416. CrossRef
Detection and Determination ofToxoplasma gondiiSeroprevalence in Native Korean Goats (Capra hircus coreanae) Byeong Yeal Jung, Eyerusalem Bizuneh Gebeyehu, Seung-Hun Lee, Min-Goo Seo, Jae-Won Byun, Jae Ku Oem, Ha-Young Kim, Dongmi Kwak Vector-Borne and Zoonotic Diseases.2014; 14(5): 374. CrossRef
Prevalence of Intestinal Parasitic Infections Among Rural Inhabitants of Hamadan City, Iran, 2012 Rasool Jafari, Mohammad Fallah, Hossein Yousofi Darani, Hosein Ali Yousefi, Mohammad Ali Mohaghegh, Milad Latifi, Mohammad Sadaghian, Amir Hossein Maghsood Avicenna Journal of Clinical Microbiology and Infection.2014; 1(2): 21445. CrossRef
The origins of human parasites: Exploring the evidence for endoparasitism throughout human evolution Piers D. Mitchell International Journal of Paleopathology.2013; 3(3): 191. CrossRef
Intestinal Parasites among Wild Rodents in Northern Gangwon-do, Korea Young-Il Lee, Hee-Jang Pyeon, Min Seo The Korean Journal of Parasitology.2013; 51(5): 603. CrossRef
Positivity and Intensity of Gnathostoma spinigerum Infective Larvae in Farmed and Wild-Caught Swamp Eels in Thailand Wilai Saksirisampant, Benjamas Wongsatayanon Thanomsub The Korean Journal of Parasitology.2012; 50(2): 113. CrossRef
An Unusual Case of Hypereosinophilia and Abdominal Pain: An Outbreak ofTrichostrongylusImported From New Zealand Emma C. Wall, Neha Bhatnagar, Julie Watson, Tom Doherty Journal of Travel Medicine.2011; 18(1): 59. CrossRef
Zoonotic enteric protozoa R.C.A. Thompson, A. Smith Veterinary Parasitology.2011; 182(1): 70. CrossRef
Pneumocystis: from a doubtful unique entity to a group of highly diversified fungal species Magali Chabé, Cécile-Marie Aliouat-Denis, Laurence Delhaes, El Moukhtar Aliouat, Eric Viscogliosi, Eduardo Dei-Cas FEMS Yeast Research.2011; 11(1): 2. CrossRef
First
Prev
