Parasites, Hosts and Diseases

"Jae-Ho Lee"

Original Articles

Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts: Hae-Jin Sohn, Ga-Eun Seo, Jae-Ho Lee, A-Jeong Ham, Young-Hwan Oh, Heekyoung Kang, Ho-Joon Shin; Korean J Parasitol 2019;57(3):217-223.
Published online June 30, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.3.217

Abstract
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Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this study, we observed the cytopathic effect, in vitro cytotoxicity, and secretion pattern of cytokines in human corneal epithelial cells (HCECs) induced by A. castellanii trophozoites and/or cysts. Morphological observation revealed that panked dendritic HCECs co-cultured with amoeba cysts had changed into round shape and gradually died. Such changes were more severe in co-culture with cyst than those of co-cultivation with trophozoites. In vitro cytotoxicity assay revealed the highest cytotoxicity to HCECs in the co-culture system with amoeba cysts. A. castellanii induced the expression of IL-1α, IL-6, IL-8, and CXCL1 in HCECs. Secreted levels of IL-1α, IL-6, and IL-8 in HCECs co-cultured with both trophozoites and cysts were increased at an early incubation time (3 and 6 hr). These results suggested that cytopathic changes and pro-inflammatory cytokines release of HCECs in response to A. castellanii, especially amoebic cysts, are an important mechanism for AK development.

Citations

Citations to this article as recorded by

A Synthetic View on Acanthamoeba Keratitis Host Immune Response: Potential Factors Influencing the Development of Chronic Inflammation
Bianca Prado-Costa, Larissa Fagundes Pinto, Mariana Fernandes Fonseca, Denise de Freitas, Larissa Magalhães Alvarenga
Cornea.2025; 44(1): 118. CrossRef
In Vitro Efficacy of Miltefosine Against Clinical Isolates of Acanthamoeba spp. from Patients with Keratitis
Lakshminarayanan Gowtham, Savitri Sharma, Bhupesh Bagga
Seminars in Ophthalmology.2025; 40(8): 767. CrossRef
Diagnostic features of Acanthamoeba keratitis via in vivo confocal microscopy
Joanna Przybek-Skrzypecka, Malcolm Armstrong, Jennifer Kim, Andrew Walkden, Leon Au, Arun Brahma, Fiona Carley, Jaya Devi Chidambaram
Scientific Reports.2025;[Epub] CrossRef
Assessing Acanthamoeba cytotoxicity: comparison of common cell viability assays
Alvie Loufouma Mbouaka, Iwona Lesiak-Markowicz, Irene Heredero-Bermejo, Rounik Mazumdar, Julia Walochnik, Tania Martín-Pérez
Frontiers in Microbiology.2023;[Epub] CrossRef
Host cell-type and pathogen-specific immunomodulatory functions of macrophage migration inhibitory factor (MIF) in infectious keratitis
Swagata Ghosh, AH Humera Khathun, G.S. Athulya, P. Vignesh, L Mathan, Ninad Mudaraddi, Siddharth Narendran, Prajna Lalitha, N. Venkatesh Prajna
Experimental Eye Research.2023; 236: 109669. CrossRef
Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP
Qingshan Ji, Lisong Wang, Jiajia Liu, Yali Wu, Huayi Lv, Yuechun Wen, Lei Shi, Bin Qu, Nóra Szentmáry
Inflammation.2021; 44(2): 682. CrossRef
Corneal Changes in Acanthamoeba Keratitis at Various Levels of Severity: An In Vivo Confocal Microscopic Study
Zhenyu Wei, Kai Cao, Leying Wang, Christophe Baudouin, Antoine Labbé, Qingfeng Liang
Translational Vision Science & Technology.2021; 10(7): 10. CrossRef
Polymicrobial Keratitis: Risk Factors, Clinical Characteristics, Bacterial Profile, and Antimicrobial Resistance
Laura A. González-Dibildox, José A. Oyervidez-Alvarado, Kristian A. Vazquez-Romo, Nallely Ramos-Betancourt, Everardo Hernandez-Quintela, Francisco Beltran, Manuel Garza-Leon
Eye & Contact Lens: Science & Clinical Practice.2021; 47(8): 465. CrossRef

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Effects of iNOS inhibitor on IFN-γ production and apoptosis of splenocytes in genetically different strains of mice infected with Toxoplasma gondii: Ki-Man Kang, Gye-Sung Lee, Jae-Ho Lee, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2004;42(4):175-183.
Published online December 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.4.175

Abstract
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To evaluate the role of nitric oxide (NO) in IFN-γ production and apoptosis of splenocytes in genetically different strains of mice with toxoplasmosis, BALB/c (a toxoplasmosis resistant strain) and C57BL/6 (a toxoplasmosis susceptible strain) mice were infected with Toxoplasma gondii cysts orally and subsequently injected intraperitoneally with aminoguanidine, an iNOS inhibitor (AG; 35 mg/kg per mouse daily for 14 days). When BALB/c or C57BL/6 mice were infected with T. gondii without AG treatment, number of brain cysts, NO and IFN-γ production by splenocytes, and percentages of apoptotic splenocytes were increased compared to uninfected control mice without AG treatment. AG treatment increased the number of brain cysts, and reduced NO and IFN-γ production in T. gondii-infected C57BL/6 mice. In contrast, in T. gondii-infected BABL/c mice, the number of brain cysts, and NO and IFN-γ production of splenocytes was not altered by treatment with AG. However, the percentages of apoptotic splenocytes in T. gondii-infected BALB/c or C57BL/6 mice were not affected by AG treatment. These results suggest that NO modulates IFN-γ production in T. gondii-infected C57BL/6 mice, and that NO is involved in mediating a protective response in toxoplasmosis susceptible, but not resistant, mice strain during acute infection.

Citations

Citations to this article as recorded by

Effect of spiramycin versus aminoguanidine and their combined use in experimental toxoplasmosis
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BMC Immunology.2011;[Epub] CrossRef
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