Parasites, Hosts and Diseases

"Jeong-Keun Lee"

Original Articles

Influencing Factors for Cure of Clonorchiasis by Praziquantel Therapy: Infection Burden and CYP3A5 Gene Polymorphism: Chung Hyeon Kim, Jeong-Keun Lee, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2011;49(1):45-49.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.45

Abstract
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Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2±3,600.0 vs 4,998.5±7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs≥1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs≥1,000.

Citations

Citations to this article as recorded by

Clonorchiasis and opisthorchiasis: epidemiology, transmission, clinical features, morbidity, diagnosis, treatment, and control
Men-Bao Qian, Jennifer Keiser, Jürg Utzinger, Xiao-Nong Zhou, Graeme N. Forrest
Clinical Microbiology Reviews.2024;[Epub] CrossRef
Efficacy and Safety of Tribendimidine Against Clonorchis sinensis
Men-Bao Qian, Peiling Yap, Yi-Chao Yang, Hai Liang, Zhi-Hua Jiang, Wei Li, Yu-Guang Tan, Hui Zhou, Jürg Utzinger, Xiao-Nong Zhou, Jennifer Keiser
Clinical Infectious Diseases.2013; 56(7): e76. CrossRef
Efficacy of Praziquantel against Schistosoma mekongi and Opisthorchis viverrini: A Randomized, Single-Blinded Dose-Comparison Trial
Leonore Lovis, Tippi K. Mak, Khampheng Phongluxa, Phonepasong Ayé Soukhathammavong, Youthanavanh Vonghachack, Jennifer Keiser, Penelope Vounatsou, Marcel Tanner, Christoph Hatz, Jürg Utzinger, Peter Odermatt, Kongsap Akkhavong, Banchob Sripa
PLoS Neglected Tropical Diseases.2012; 6(7): e1726. CrossRef

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63 Download
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Single Nucleotide Polymorphisms of Cytokine Genes are Associated with Fibrosis of the Intrahepatic Bile Duct Wall in Human Clonorchiasis: Byung-Suk Chung, Jeong-Keun Lee, Min-Ho Choi, Myoung Hee Park, Dongil Choi, Sung-Tae Hong; Korean J Parasitol 2009;47(2):145-151.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.145

Abstract
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This study examined the association of cytokine gene polymorphisms with intrahepatic bile duct wall fibrosis in human clonorchiasis. A total of 240 residents in Heilongjiang, China underwent ultrasonography, blood sampling, and stool examination. Single nucleotide polymorphism (SNP) sites for IFN-γ (+874 T/A), IL-10 (-1,082 G/A, -819 C/T, -592 C/A), TNF-α (-308 G/A), and TGF-β1 (codon 10 T/C, codon 25 G/C) genes were observed with the TaqMan allelic discrimination assay. No significant correlation was observed between individual cytokine gene polymorphisms and intrahepatic duct dilatation (IHDD). Among individuals with clonorchiasis of moderate intensity, the incidence of IHDD was high in those with IFN-γ intermediate-producing genotype, +874AT (80.0%, P = 0.177), and in those with TNF-α low-producing genotype, -308GG (63.0%, P = 0.148). According to the combination of IFN-γ and TNF-α genotypes, the risks for IHDD could be stratified into high (intermediate-producing IFN-γ and low producing TNF-α), moderate, and low (low-producing IFN-γ and high producing TNF-α) risk groups. The incidence of IHDD was significantly different among these groups (P = 0.022): 88.9% (odds ratio, OR = 24.0) in high, 56.5% (OR = 3.9) in moderate, and 25.0% (OR = 1) in low risk groups. SNP of IFN-γ and TNF-α genes may contribute to the modulation of fibrosis in the intrahepatic bile duct wall in clonorchiasis patients.

Citations

Citations to this article as recorded by

Risk Factors of Clonorchis sinensis Human Infections in Endemic Areas, Haman-Gun, Republic of Korea: A Case-Control Study
Sang-Eun Lee, Hee-Eun Shin, Myoung-Ro Lee, Yang-Hee Kim, Shin-Hyeong Cho, Jung-Won Ju
The Korean Journal of Parasitology.2020; 58(6): 647. CrossRef
The impact of cytokine gene polymorphisms on Epstein–Barr virus infection outcome in pediatric liver transplant recipients
Beata Kasztelewicz, Irena Jankowska, Joanna Pawłowska, Joanna Teisseyre, Katarzyna Dzierżanowska-Fangrat
Journal of Clinical Virology.2012; 55(3): 226. CrossRef
Clinical relevance of the interleukin 10 promoter polymorphisms in Chinese Han patients with major trauma: genetic association studies
Ling Zeng, Wei Gu, Kehong Chen, Dongpo Jiang, Lianyang Zhang, Dingyuan Du, Ping Hu, Qing Liu, Suna Huang, Jianxin Jiang
Critical Care.2009;[Epub] CrossRef

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Brief Communication

Collection of Clonorchis sinensis adult worms from infected humans after praziquantel treatment: Chenghua Shen, Jae-hwan Kim, Jeong-Keun Lee, Young Mee Bae, Min-Ho Choi, Jin-Kyoung Oh, Min Kyung Lim, Hai-Rim Shin, Sung-Tae Hong; Korean J Parasitol 2007;45(2):149-152.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.149

Abstract
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A cohort was established for evaluation of cancer risk factors in Sancheong-gun, Gyeongsangnam-do, Korea. As one of the cohort studies, stools of 947 residents (403 males and 544 females, age range: 29-86 years) were screened for Clonorchis sinensis eggs using both Kato-Katz method and formalin-ether sedimentation technique. The overall egg positive rate of C. sinensis was 37.7% and individual EPG (eggs per gram of feces) counts ranged from 24 to 28,800. Eight egg positive residents voluntarily joined a process of collection of the passed worms after praziquantel treatment. A total of 158 worms were recovered from 5 of the 8 treated persons, ranged from 3 to 108 in each individual. The worms were 15-20 mm × 2-3 mm in size, and showed brown-pigmented, red, or white body colors. This is the first collection record of C. sinensis adult worms from humans through anthelmintic treatment and purgation. The adult worms of C. sinensis may be paralyzed by praziquantel and then discharged passively through bile flow in the bile duct and by peristaltic movement of the bowel.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
Natural history of liver fluke infection underpins epidemiological patterns of biliary cancer
Thomas Crellen, Francesca Vita, Chiara Braconi, Paiboon Sithithaworn, T. Déirdre Hollingsworth
Proceedings of the National Academy of Sciences.2025;[Epub] CrossRef
Diagnosis of helminths depends on worm fecundity and the distribution of parasites within hosts
Thomas Crellen, Melissa Haswell, Paiboon Sithithaworn, Somphou Sayasone, Peter Odermatt, Poppy H. L. Lamberton, Simon E. F. Spencer, T. Déirdre Hollingsworth
Proceedings of the Royal Society B: Biological Sciences.2023;[Epub] CrossRef
A comparison of helminth infections as assessed through coprological analysis and adult worm burdens in a wild host
Rachel L. Byrne, Ursula Fogarty, Andrew Mooney, Nicola M. Marples, Celia V. Holland
International Journal for Parasitology: Parasites and Wildlife.2018; 7(3): 439. CrossRef
Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples
S. M. Mazidur Rahman, Hyun Beom Song, Yan Jin, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Min-Ho Choi, Justin V. Remais
PLOS Neglected Tropical Diseases.2017; 11(10): e0005995. CrossRef
Cohort Profile: Community-based prospective cohort from the National Cancer Center, Korea
Jin-Kyoung Oh, Min Kyung Lim, E Hwa Yun, Min-Ho Choi, Sung-Tae Hong, Soung-Hoon Chang, Sue Kyung Park, Sung-Il Cho, Dong-Hyun Kim, Keun-Young Yoo, Hai-Rim Shin
International Journal of Epidemiology.2015; : dyv302. CrossRef
An EF-handed Ca2+-binding protein of Chinese liver fluke Clonorchis sinensis
Eun Joo Chung, Tae Yun Kim, Sung-Jong Hong, Tai-Soon Yong
Parasitology Research.2013; 112(12): 4121. CrossRef
Clonorchis sinensis and clonorchiasis, an update
Sung-Tae Hong, Yueyi Fang
Parasitology International.2012; 61(1): 17. CrossRef
Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats
S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Parasitology International.2012; 61(1): 203. CrossRef
Oriental Cholangiohepatitis (Clonorchiasis Infestation) Caused by Clonorchis Sinensis
Sarah Klemencic, Michael Phelan, Robert Patrick, Noushin Vahdat
The Journal of Emergency Medicine.2012; 43(2): e107. CrossRef
Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis
S. M. Mazidur Rahman, Young Mee Bae, Sung-Tae Hong, Min-Ho Choi
Parasitology Research.2011; 109(2): 297. CrossRef
Hypersensitive Reaction to Praziquantel in a Clonorchiasis Patient
Jung-Min Lee, Hyun-Sul Lim, Sung-Tae Hong
The Korean Journal of Parasitology.2011; 49(3): 273. CrossRef
Obstructive Jaundice Caused by Clonorchiasis-associated Duodenal Papillitis: A Case Report
Jun Uk Lim, Kwang Ro Joo, Hyun Phil Shin, Jae Myung Cha, Joung Il Lee, Sung Jig Lim
Journal of Korean Medical Science.2011; 26(1): 135. CrossRef
Correlation between Discharged Worms and Fecal Egg Counts in Human Clonorchiasis
Jae-Hwan Kim, Min-Ho Choi, Young Mee Bae, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Akira Ito
PLoS Neglected Tropical Diseases.2011; 5(10): e1339. CrossRef