Parasites, Hosts and Diseases

"Jong-Il Kim"

Original Article

Draft Genome of Toxocara canis, a Pathogen Responsible for Visceral Larva Migrans: Jinhwa Kong, Jungim Won, Jeehee Yoon, UnJoo Lee, Jong-Il Kim, Sun Huh; Korean J Parasitol 2016;54(6):751-758.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.751

Abstract
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This study aimed at constructing a draft genome of the adult female worm Toxocara canis using next-generation sequencing (NGS) and de novo assembly, as well as to find new genes after annotation using functional genomics tools. Using an NGS machine, we produced DNA read data of T. canis. The de novo assembly of the read data was performed using SOAPdenovo. RNA read data were assembled using Trinity. Structural annotation, homology search, functional annotation, classification of protein domains, and KEGG pathway analysis were carried out. Besides them, recently developed tools such as MAKER, PASA, Evidence Modeler, and Blast2GO were used. The scaffold DNA was obtained, the N50 was 108,950 bp, and the overall length was 341,776,187 bp. The N50 of the transcriptome was 940 bp, and its length was 53,046,952 bp. The GC content of the entire genome was 39.3%. The total number of genes was 20,178, and the total number of protein sequences was 22,358. Of the 22,358 protein sequences, 4,992 were newly observed in T. canis. Following proteins previously unknown were found: E3 ubiquitin-protein ligase cbl-b and antigen T-cell receptor, zeta chain for T-cell and B-cell regulation; endoprotease bli-4 for cuticle metabolism; mucin 12Ea and polymorphic mucin variant C6/1/40r2.1 for mucin production; tropomodulin-family protein and ryanodine receptor calcium release channels for muscle movement. We were able to find new hypothetical polypeptides sequences unique to T. canis, and the findings of this study are capable of serving as a basis for extending our biological understanding of T. canis.

Citations

Citations to this article as recorded by

Parasites in Sewage: Legal Requirements and Diagnostic Tools
Oliwia Obuch-Woszczatyńska, Klaudia Bylińska, Małgorzata Krzyżowska, Karol Korzekwa, Piotr Bąska
Pathogens.2025; 14(1): 86. CrossRef
Toward anthelmintic drug candidates for toxocariasis: Challenges and recent developments
Ana C. Mengarda, Tais C. Silva, Aline S. Silva, Daniel B. Roquini, João Paulo S. Fernandes, Josué de Moraes
European Journal of Medicinal Chemistry.2023; 251: 115268. CrossRef
Antigenic Proteins from the Excretory–Secretory Products of Toxocara canis Larvae and Evaluation of Their Potential for Immunodiagnostics of Larval Toxocarosis
Kateřina Skulinová, Jan Novák, Libuše Kolářová, Martin Kašný
Acta Parasitologica.2022; 67(2): 705. CrossRef
GAAP: A Genome Assembly + Annotation Pipeline
Jinhwa Kong, Sun Huh, Jung-Im Won, Jeehee Yoon, Baeksop Kim, Kiyong Kim
BioMed Research International.2019; 2019: 1. CrossRef

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Brief Communication

Intestinal protozoan infections and echinococcosis in the inhabitants of Dornod and Selenge, Mongolia (2003): Sun Huh, Jae-Ran Yu, Jong-Il Kim, Choijamts Gotov, Radnaabazar Janchiv, Jeong-Sun Seo; Korean J Parasitol 2006;44(2):171-174.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.171

Abstract
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The status of intestinal parasitic infections and seroprevalence of cystic echinococcosis in the inhabitants in Dornod and Selenge, Mongolia, was observed with stool and serum samples from 165 and 683 residents from August 9 to August 15, 2003. A total of 10 inhabitants (6.1%) were found to be infected with protozoan cysts or oocysts by stool examinations; 7, 1 and 2 cases with Entamoeba coli, Giardia lamblia and Cryptosporidium parvum, respectively. A total of 62 people (9.1%) revealed significantly high levels of specific antibodies against Echinococcus granulosus by enzyme-linked immunosorbent assay (ELISA). The surveyed areas are rural areas and the sanitized tab water is not available. There is a possibility of endemic transmission of water-borne protozoan diseases in these areas. The clinical echinococcosis is needed to be searched from the seropositive inhabitants.

Citations

Citations to this article as recorded by

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