Parasites, Hosts and Diseases

"Kyung Ho Kim"

Brief Communication

Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing: Ji Sun Jang, Kyung Ho Kim, Jae-Ran Yu, Soo-Ung Lee; Korean J Parasitol 2007;45(4):301-306.
Published online December 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.4.301

Abstract
PDF

We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.

Citations

Citations to this article as recorded by

Clonorchis sinensis infection are associated with calcium phosphate gallbladder stones: A single-center retrospective observational study in China
Rui-Hong Ma, Xiao-Bing Luo, Yu Peng Liu, Qin Li
Medicine.2025; 104(46): e45739. CrossRef
Gallstone and Gallbladder Disease: Biliary Tract and Cholangiopathies
Ludovica Ceci, Yuyan Han, Kelsey Krutsinger, Leonardo Baiocchi, Nan Wu, Debjyoti Kundu, Konstantina Kyritsi, Tianhao Zhou, Eugenio Gaudio, Heather Francis, Gianfranco Alpini, Lindsey Kennedy
Comprehensive Physiology.2023; 13(3): 4909. CrossRef
A systematic study on 33 gallbladder stones resembling adult Clonorchis sinensis worms
R.-h. Ma, X.-f. Wang, Q. Li, H.-y. Cai, X.-b. Luo
Journal of Helminthology.2022;[Epub] CrossRef
Clonorcis sinensis eggs are associated with calcium carbonate gallbladder stones
Tie Qiao, Rui-hong Ma, Zhen-liang Luo, Liu-qing Yang, Xiao-bing Luo, Pei-ming Zheng
Acta Tropica.2014; 138: 28. CrossRef
Molecular diagnosis in clinical parasitology: When and why?
Samson SY Wong, Kitty SC Fung, Sandy Chau, Rosana WS Poon, Sally CY Wong, Kwok-Yung Yuen
Experimental Biology and Medicine.2014; 239(11): 1443. CrossRef
A microstructural study of gallbladder stones using scanning electron microscopy
Tie Qiao, Rui‐Hong MA, Xiao‐Bing Luo, Zhen‐Liang Luo, Pei‐Ming Zheng, Liu‐Qing Yang
Microscopy Research and Technique.2013; 76(5): 443. CrossRef
The zoonotic, fish-borne liver flukes Clonorchis sinensis, Opisthorchis felineus and Opisthorchis viverrini
Trevor N. Petney, Ross H. Andrews, Weerachai Saijuntha, Alexandra Wenz-Mücke, Paiboon Sithithaworn
International Journal for Parasitology.2013; 43(12-13): 1031. CrossRef
Molecular Diagnostics and Parasitic Disease
Shawn Vasoo, Bobbi S. Pritt
Clinics in Laboratory Medicine.2013; 33(3): 461. CrossRef
Microscopic Examination of Gallbladder Stones Improves Rate of Detection of Clonorchis sinensis Infection
Tie Qiao, Rui-hong Ma, Xiao-bing Luo, Pei-ming Zheng, Zhen-liang Luo, Liu-qing Yang
Journal of Clinical Microbiology.2013; 51(8): 2551. CrossRef
New pathophysiological concepts underlying pathogenesis of pigment gallstones
Libor Vítek, Martin C. Carey
Clinics and Research in Hepatology and Gastroenterology.2012; 36(2): 122. CrossRef
Development of a real-time PCR assay for the detection of Clonorchis sinensis DNA in gallbladder bile and stone samples from patients with cholecystolithiasis
Tie Qiao, Pei-Ming Zheng, Rui-Hong Ma, Xiao-Bing Luo, Zhen-Liang Luo
Parasitology Research.2012; 111(4): 1497. CrossRef
Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
Parasitology International.2012; 61(1): 71. CrossRef
Trematode Infections
Thomas Fürst, Urs Duthaler, Banchop Sripa, Jürg Utzinger, Jennifer Keiser
Infectious Disease Clinics of North America.2012; 26(2): 399. CrossRef
Concept of the pathogenesis and treatment of cholelithiasis
Vasiliy Ivanovich Reshetnyak
World Journal of Hepatology.2012; 4(2): 18. CrossRef
Update on the diagnosis and treatment of food-borne trematode infections
Jennifer Keiser, Urs Duthaler, Jürg Utzinger
Current Opinion in Infectious Diseases.2010; 23(5): 513. CrossRef