Innate lymphoid cells (ILCs) are key players during an immune response at the mucosal surfaces, such as lung, skin, and gastrointestinal tract. Giardia lamblia is an extracellular protozoan pathogen that inhabits the human small intestine. In this study, ILCs prepared from the lamina propria of mouse small intestine were incubated with G. lamblia trophozoites. Transcriptional changes in G. lamblia-exposed ILCs resulted in identification of activation of several immune pathways. Secretion of interleukin (IL)-17A, IL-17F, IL-1β, and interferon-γ was increased, whereas levels of IL-13, IL-5, and IL22, was maintained or reduced upon exposure to G. lamblia. Goup 3 ILC (ILC3) was found to be dominant amongst the ILCs, and increased significantly upon co-cultivation with G. lamblia trophozoites. Oral inoculation of G. lamblia trophozoites into mice resulted in their presence in the small intestine, of which, the highest number of parasites was detected at the 5 days-post infection. Increased ILC3 was observed amongst the ILC population at the 5 days-post infection. These findings indicate that ILC3 from the lamina propria secretes IL-17 in response to G. lamblia, leading to the intestinal pathology observed in giardiasis.
Citations
Citations to this article as recorded by
Post-stroke depression: exploring gut microbiota-mediated barrier dysfunction through immune regulation Jia Jiang, Haihua Xie, Sihui Cao, Xuan Xu, Jingying Zhou, Qianyan Liu, Changsong Ding, Mi Liu Frontiers in Immunology.2025;[Epub] CrossRef
Giardia duodenalis stabilizes HIF-1α and induces glycolytic alterations in intestinal epithelial cells Emily DeMichele, Olivia Sosnowski, Darragh Flood, Cormac T. Taylor, Ian A. Lewis, Thibault Allain, Andre G. Buret Scientific Reports.2025;[Epub] CrossRef
Unravelling the cellular sources and location of IL-17A production during a Giardia infection Charlotte E. Van Crombrugge, Bregt Decorte, Leen J. M. Seys, Peter Geldhof Scientific Reports.2025;[Epub] CrossRef
Microbiota, metabolites and mucosal immunity as potential targets of traditional Chinese medicine for respiratory diseases based on the lung-gut crosstalk Wei Wang, Shaoqing Zhu, Yuanbin Zhang, Lin Chu, Siyao Liu, Huan Yang, Hao Wu Pharmacological Research - Modern Chinese Medicine.2024; 10: 100374. CrossRef
Conserved Candidate Antigens and Nanoparticles to Develop Vaccine against Giardia intestinalis Suthinee Sangkanu, Alok K. Paul, Julalak Chuprom, Watcharapong Mitsuwan, Rachasak Boonhok, Maria de Lourdes Pereira, Sonia Marlene Rodrigues Oliveira, Polrat Wilairatana, Mohammed Rahmatullah, Christophe Wiart, Muhammad Nawaz, Chea Sin, Sunil Kayesth, Vee Vaccines.2022; 11(1): 96. CrossRef
Probiotics in the management of Giardia duodenalis: an update on potential mechanisms and outcomes Nasrin Dashti, Mitra Zarebavani Naunyn-Schmiedeberg's Archives of Pharmacology.2021; 394(9): 1869. CrossRef
Type 3 Innate Lymphoid Cells as Regulators of the Host-Pathogen Interaction Ana Valle-Noguera, Anne Ochoa-Ramos, Maria José Gomez-Sánchez, Aranzazu Cruz-Adalia Frontiers in Immunology.2021;[Epub] CrossRef
The role of innate lymphoid cells in response to microbes at mucosal surfaces Goo-Young Seo, Daniel A. Giles, Mitchell Kronenberg Mucosal Immunology.2020; 13(3): 399. CrossRef
Physiological function and regulatory signal of intestinal type 3 innate lymphoid cell(s) Yin Xian, Xiaodong Lv, Minjia Xie, Fuyang Xiao, Chenyang Kong, Yixing Ren Life Sciences.2020; 262: 118504. CrossRef
First
Prev
