To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons. The PCR products were cloned and sequenced, after which 12 different retrotransposon-related sequences were isolated from the trematode genome. These showed various degrees of identity to the polyprotein of divergent retrotransposon families. A phylogenetic analysis demonstrated that these sequences could be classified into three different families of LTR retrotransposons, namely, Xena, Bel, and Gypsy families. Of these, two mRNA transcripts were detected by reverse transcriptase-PCR, showing that these two elements preserved their mobile activities. The genomic distributions of these two sequences were found to be highly repetitive. These results suggest that there are diverse retrotransposons including the ancient Xena family in the genome of P. westermani, which may have been involved in the evolution of the host genome.
Citations
Citations to this article as recorded by
Gene diversity and genetic variation in lung flukes (genusParagonimus) David Blair, Yukifumi Nawa, Makedonka Mitreva, Pham Ngoc Doanh Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 6. CrossRef
Transfection of Platyhelminthes Bárbara Moguel, Raúl J. Bobes, Julio C. Carrero, Juan P. Laclette BioMed Research International.2015; 2015: 1. CrossRef
Transient transgenesis of the tapeworm Taenia crassiceps Bárbara Moguel, Norma Moreno-Mendoza, Raúl J. Bobes, Julio C. Carrero, Jesús Chimal-Monroy, Martha E. Díaz-Hernández, Luis Herrera-Estrella, Juan P. Laclette SpringerPlus.2015;[Epub] CrossRef
Review Article: ISTR, a Retrotransposons-Based Marker to Assess Plant Genome Variability with Special Emphasis in the Genera <i>Zea</i> and <i>Agave</i> Martha Isabel Torres-Morán, Norma Almaraz-Abarca, Martha Escoto-Delgadillo American Journal of Plant Sciences.2012; 03(12): 1820. CrossRef
PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy Young-An Bae, Jong-Sook Ahn, Seon-Hee Kim, Mun-Gan Rhyu, Yoon Kong, Seung-Yull Cho BMC Genomics.2008;[Epub] CrossRef
Both sense and antisense strands of the LTR of the Schistosoma mansoni Pao-like retrotransposon Sinbad drive luciferase expression Claudia S. Copeland, Victoria H. Mann, Paul J. Brindley Molecular Genetics and Genomics.2007; 277(2): 161. CrossRef
The evolutionary course of the CsRn1 long-terminal-repeat (LTR) retrotransposon was predicted by conducting a phylogenetic analysis with its paralog LTR sequences. Based on the clustering patterns in the phylogenetic tree, multiple CsRn1 copies could be grouped into four subsets, which were shown to have different integration times. Their differential sequence divergences and heterogeneous integration patterns strongly suggested that these subsets appeared sequentially in the genome of C. sinensis. Members of recently expanding subset showed the lowest level of divergence in their LTR and reverse transcriptase gene sequences. They were also shown to be highly polymorphic among individual genomes of the trematode. The CsRn1 element exhibited a preference for repetitive, agenic chromosomal regions in terms of selecting integration targets. Our results suggested that CsRn1 might induce a considerable degree of intergenomic variation and, thereby, have influenced the evolution of the C. sinensis genome.
Citations
Citations to this article as recorded by
Recurrent emergence of structural variants of LTR retrotransposon CsRn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, Clonorchis sinensis Seon-Hee Kim, Yoon Kong, Young-An Bae Molecular and Biochemical Parasitology.2017; 214: 14. CrossRef
Genomics and transcriptomics of the Chinese liver fluke Clonorchis sinensis (Opisthorchiidae, Trematoda) G. N. Chelomina Molecular Biology.2017; 51(2): 184. CrossRef
Egg-specific expression of protein with DNA methyltransferase activity in the biocarcinogenic liver fluke Clonorchis sinensis SEON-HEE KIM, HYE-JEONG CHO, WOON-MOK SOHN, CHUN-SEOB AHN, YOON KONG, HYUN-JONG YANG, YOUNG-AN BAE Parasitology.2015; 142(9): 1228. CrossRef
Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser Biotechnology Advances.2010; 28(6): 859. CrossRef
The biology and evolution of transposable elements in parasites M. Carmen Thomas, Francisco Macias, Carlos Alonso, Manuel C. López Trends in Parasitology.2010; 26(7): 350. CrossRef
Functional Genes and Proteins of Clonorchis sinensis Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Molecular genetic profiles among individual Clonorchis sinensis adults collected from cats in two geographic regions of China revealed by RAPD and MGE-PCR methods De-Hua Lai, Qiao-Ping Wang, Wen Chen, Lian-Shun Cai, Zhong-Dao Wu, Xing-Quan Zhu, Zhao-Rong Lun Acta Tropica.2008; 107(2): 213. CrossRef
PwRn1, a novel Ty3/gypsy-like retrotransposon of Paragonimus westermani: molecular characters and its differentially preserved mobile potential according to host chromosomal polyploidy Young-An Bae, Jong-Sook Ahn, Seon-Hee Kim, Mun-Gan Rhyu, Yoon Kong, Seung-Yull Cho BMC Genomics.2008;[Epub] CrossRef
First
Prev
