Parasites, Hosts and Diseases

"Leishmania major"

Original Articles

Tamoxifen Induces Apoptosis of Leishmania major Promastigotes in Vitro: Masoud Doroodgar, Mahdi Delavari, Moein Doroodgar, Ali Abbasi, Ali Akbar Taherian, Abbas Doroodgar; Korean J Parasitol 2016;54(1):9-14.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.9

Abstract
PDF

Tamoxifen is an antagonist of the estrogen receptor and currently used for the treatment of breast cancer. The current treatment of cutaneous leishmaniasis with pentavalent antimony compounds is not satisfactory. Therefore, in this study, due to its antileishmanial activity, effects of tamoxifen on the growth of promastigotes and amastigotes of Leishmania major Iranian strain were evaluated in vitro. Promastigotes and amastigotes were treated with different concentrations (1, 5, 10, 20, and 50 μg/ml) and time periods (24, 48, and 72 hr) of tamoxifen. After tamoxifen treatment, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5 biphenyl tetrazolium bromide assay) was used to determine the percentage of live parasites and Graph Pad Prism software to calculate IC₅₀. Flow cytometry was applied to investigate the induction of tamoxifen-induced apoptosis in promastigotes. The half maximal inhibitory concentration (IC₅₀) of tamoxifen on promastigotes was 2.6 μg/ml after 24 hr treatment. Flow cytometry analysis showed that tamoxifen induced early and late apoptosis in Leishmania promastigotes. While after 48 hr in control group the apoptosis was 2.0%, the 50 ?g/L concentration of tamoxifen increased it to 59.7%. Based on the in vitro antileishmanial effect, tamoxifen might be used for leishmaniasis treatment; however, further researches on in vivo effects of tamoxifen in animal models are needed.

Citations

Citations to this article as recorded by

Therapeutic effect of topical tamoxifen in murine cutaneous leishmaniasis caused by Leishmania major
Parastoo Hassani-Abharian, Seyedamirmehdi Hejazi Dehaghani, Zabihollah Shahmoradi, Seyed Hossein Hejazi, Audrey Odom John
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Compared Antileishmanial Activity of Clomiphene and Tamoxifen
Sergio Sifontes-Rodríguez, Alma Reyna Escalona-Montaño, Ricardo Mondragón Flores, Niurka Mollineda-Diogo, Lianet Monzote Fidalgo, Mónica Edith Mondragón-Castelán, Fedra Alardin-Gutiérrez, Lourdes Araceli López-Enzana, Daniel Andrés Sánchez-Almaraz, Ofelia
Biomedicines.2024; 12(10): 2290. CrossRef
Caryocar coriaceumWittm. fruit extracts asLeishmaniainhibitors:in-vitroandin-silicoapproaches
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Selene Maia de Morais, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Taylon Felipe Silva, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Idessania Nazareth Costa, Emmanuel Silva Marinho, Mar
Journal of Biomolecular Structure and Dynamics.2022; 40(17): 8040. CrossRef
Antileishmanial Activity of Tamoxifen by Targeting Sphingolipid Metabolism: A Review
Kaleab Alemayehu Zewdie, Haftom Gebregergs Hailu, Muluken Altaye Ayza, Bekalu Amare Tesfaye
Clinical Pharmacology: Advances and Applications.2022; Volume 14: 11. CrossRef
The potential role and apoptotic profile of three medicinal plant extracts on Leishmania tropica by MTT assay, macrophage model and flow cytometry analysis
Mozhde Ilaghi, Iraj Sharifi, Fariba Sharififar, Fatemeh Sharifi, Razieh Tavakoli Oliaee, Zahra Babaei, Manzume Shamsi Meimamandi, Alireza Keyhani, Mehdi Bamorovat
Parasite Epidemiology and Control.2021; 12: e00201. CrossRef
Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
Jasem Saki, Khalil Saki, Reza Arjmand
Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub] CrossRef
Selective Estrogen Receptor Modulators Against Gram-Positive and Gram-Negative Bacteria: An Experimental Study
Aakriti Garg, Arti Singh, Anoop Kumar
Future Microbiology.2021; 16(13): 987. CrossRef
Network-Based Approaches Reveal Potential Therapeutic Targets for Host-Directed Antileishmanial Therapy Driving Drug Repurposing
J. Eduardo Martinez-Hernandez, Zaynab Hammoud, Alessandra Mara de Sousa, Frank Kramer, Rubens L. do Monte-Neto, Vinicius Maracaja-Coutinho, Alberto J. M. Martin, Tim Downing
Microbiology Spectrum.2021;[Epub] CrossRef
Male predominance in reported Visceral Leishmaniasis cases: Nature or nurture? A comparison of population-based with health facility-reported data
Kristien Cloots, Sakib Burza, Paritosh Malaviya, Epco Hasker, Sangeeta Kansal, Guy Mollett, Jaya Chakravarty, Nurpur Roy, Bibek Kumar Lal, Suman Rijal, Shyam Sundar, Marleen Boelaert, Guilherme L. Werneck
PLOS Neglected Tropical Diseases.2020; 14(1): e0007995. CrossRef
Selective Estrogen Receptor Modulators (SERMs): Mechanistic Insights Against Microbial Infections
Aakriti Garg, Balraj Singh, Ruchika Sharma, Arti Singh, Anoop Kumar
Current Molecular Medicine.2020; 20(2): 102. CrossRef
Epidemiology, Associated Factors and Treatment Methods of Cutaneous Leishmaniasis Based on Previous Data from 2013 to 2018 in Ilam, Western Iran
Nasrin Rezaee, Vahid Raissi, Ahmad Rajaeipour, Mehdi Nazari, Muhammad Getso, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim
Acta Parasitologica.2020; 65(3): 760. CrossRef
Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage
João Paulo Assolini, Fernanda Tomiotto-Pellissier, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Sergio Marques Borghi, Waldiceu Apareci
Journal of Drug Targeting.2020; 28(10): 1110. CrossRef
4-nitrochalcone exerts leishmanicidal effect on L. amazonensis promastigotes and intracellular amastigotes, and the 4-nitrochalcone encapsulation in beeswax copaiba oil nanoparticles reduces macrophages cytotoxicity
João Paulo Assolini, Thais Peron da Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Claudia Sayer, Pe
European Journal of Pharmacology.2020; 884: 173392. CrossRef
Repurposing azole antifungals into antileishmanials: Novel 3-triazolylflavanones with promising in vitro antileishmanial activity against Leishmania major
Masoud Keighobadi, Saeed Emami, Mahdi Fakhar, Azar Shokri, Hassan Mirzaei, Saeed Hosseini Teshnizi
Parasitology International.2019; 69: 103. CrossRef
Study of Ethinyl Estradiol Activity Against Promastigotes, Axenic and Macrophage-Dwelling Amastigotes of Leishmania infantum by Using Atomic Force Microscopy and Methyl Thiazolyl Tetrazolium Methods
Farnoush Shadnoush, Reza Arjmand, Fakher Rahim, Jasem Saki
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Repurposing as a strategy for the discovery of new anti-leishmanials: the-state-of-the-art
REBECCA L. CHARLTON, BARTIRA ROSSI-BERGMANN, PAUL W. DENNY, PATRICK G. STEEL
Parasitology.2018; 145(2): 219. CrossRef
Caryocar coriaceum extracts exert leishmanicidal effect acting in promastigote forms by apoptosis-like mechanism and intracellular amastigotes by Nrf2/HO-1/ferritin dependent response and iron depletion
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Milena Menegazzo Miranda-Sapla, Selene Maia de Morais, João Paulo Assolini, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Allan Henrique Depieri Cataneo, Danielle Kian, Tiago Bervelieri Ma
Biomedicine & Pharmacotherapy.2018; 98: 662. CrossRef
Epidemiological Aspects of Cutaneous Leishmaniasis during 2009-2016 in Kashan City, Central Iran
Doroodgar Moein, Doroodgar Masoud, Mahboobi Saeed, Doroodgar Abbas
The Korean Journal of Parasitology.2018; 56(1): 21. CrossRef
Tamoxifen Never Ceases to Amaze: New Findings on Non-Estrogen Receptor Molecular Targets and Mediated Effects
Tatiana Anatolievna Bogush, Boris Borisovich Polezhaev, Ivan Andreevich Mamichev, Elena Alexandrovna Bogush, Boris Evseevich Polotsky, Sergei Alexeevich Tjulandin, Andrey Borisovich Ryabov
Cancer Investigation.2018; 36(4): 211. CrossRef
Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy
Débora P. Aureliano, José Angelo Lauletta Lindoso, Sandra Regina de Castro Soares, Cleusa Fumika Hirata Takakura, Thiago Martini Pereira, Martha Simões Ribeiro
Photodiagnosis and Photodynamic Therapy.2018; 23: 1. CrossRef
Grandiflorenic acid promotes death of promastigotes via apoptosis-like mechanism and affects amastigotes by increasing total iron bound capacity
Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, João Paulo Assolini, Amanda Cristina Machado Carloto, Priscila Goes Camargo de Carvalho, Ian Lucas Alves Cardoso, Andréa Name Colado
Phytomedicine.2018; 46: 11. CrossRef
In vitro Effects of Ketotifen and Cromolyn Sodium on Promastigote and Amastigotes of Leishmania major
Lima Asgharpour Sarouey, Khadijeh Khanaliha, Parvaneh Rahimi-Moghaddam, Samaneh Khorrami, Mohammad Saaid Dayer, Fatemeh Tabatabaie
Jundishapur Journal of Microbiology.2018;[Epub] CrossRef
Repurposing Estrogen Receptor Antagonists for the Treatment of Infectious Disease
Marhiah C. Montoya, Damian J. Krysan, Danielle A. Garsin
mBio.2018;[Epub] CrossRef
Library of Seleno-Compounds as Novel Agents against Leishmania Species
Álvaro Martín-Montes, Daniel Plano, Rubén Martín-Escolano, Verónica Alcolea, Marta Díaz, Silvia Pérez-Silanes, Socorro Espuelas, Esther Moreno, Clotilde Marín, Ramón Gutiérrez-Sánchez, Carmen Sanmartín, Manuel Sánchez-Moreno
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef

10,859 View
165 Download
29 Web of Science
Crossref

In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite: Somayeh Sadeghi, Negar Seyed, Mohammad-Hossein Etemadzadeh, Saeid Abediankenari, Sima Rafati, Tahereh Taheri; Korean J Parasitol 2015;53(4):385-394.
Published online August 25, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.4.385

Abstract
PDF

Leishmaniasis is a worldwide uncontrolled parasitic disease due to the lack of effective drug and vaccine. To speed up effective drug development, we need powerful methods to rapidly assess drug effectiveness against the intracellular form of Leishmania in high throughput assays. Reporter gene technology has proven to be an excellent tool for drug screening in vitro. The effects of reporter proteins on parasite infectivity should be identified both in vitro and in vivo. In this research, we initially compared the infectivity rate of recombinant Leishmania major expressing stably enhanced green fluorescent protein (EGFP) alone or EGFP-luciferase (EGFP-LUC) with the wild-type strain. Next, we evaluated the sensitivity of these parasites to amphotericin B (AmB) as a standard drug in 2 parasitic phases, promastigote and amastigote. This comparison was made by MTT and nitric oxide (NO) assay and by quantifying the specific signals derived from reporter genes like EGFP intensity and luciferase activity. To study the amastigote form, both B10R and THP-1 macrophage cell lines were infected in the stationary phase and were exposed to AmB at different time points. Our results clearly revealed that the 3 parasite lines had similar in vitro infectivity rates with comparable parasite-induced levels of NO following interferon-γ/lipopolysaccharide induction. Based on our results we proposed the more reporter gene, the faster and more sensitive evaluation of the drug efficiency.

Citations

Citations to this article as recorded by

Characterization and Evaluation of Microwave-Synthesized Nanostructured Lipid Carriers for Enhanced Amphotericin B Efficacy Against Leishmania donovani: A Novel Therapeutic Paradigm
Sunidhi Lohan, Meenakshi Bhatia
BioNanoScience.2024; 14(3): 2782. CrossRef
Insights into the drug screening approaches in leishmaniasis
Boobalan Gopu, Parampreet Kour, Ramajayan Pandian, Kuljit Singh
International Immunopharmacology.2023; 114: 109591. CrossRef
A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry
Christian Florian Teh-Poot, Victor Manuel Dzul-Huchim, Jonathan M. Mercado, Liliana Estefanía Villanueva-Lizama, Maria Elena Bottazzi, Kathryn M. Jones, Francis T.F. Tsai, Julio Vladimir Cruz-Chan
Experimental Parasitology.2023; 249: 108519. CrossRef
Challenges and Tools for In Vitro Leishmania Exploratory Screening in the Drug Development Process: An Updated Review
Anita Cohen, Nadine Azas
Pathogens.2021; 10(12): 1608. CrossRef
A Survey on Inhibitory Effect of Whole-Body Extraction and Secretions of Lucilia sericata's Larvae on Leishmania major In vitro
Maryam Tahmasebi, Simindokht Soleimanifard, Alireza Sanei, Azadeh Karimy, Seyed Mohammad Abtahi
Advanced Biomedical Research.2020;[Epub] CrossRef
Potential of the natural products against leishmaniasis in Old World - a review of in-vitro studies
Sofia Cortes, Carolina Bruno de Sousa, Thiago Morais, João Lago, Lenea Campino
Pathogens and Global Health.2020; 114(4): 170. CrossRef
Sambucus ebulus extract stimulates cellular responses in cutaneous leishmaniasis
Maryam Heidari‐Kharaji, Vahid Fallah‐Omrani, Alireza Badirzadeh, Behnam Mohammadi‐Ghalehbin, Mohammad Ali Nilforoushzadeh, Leila Masoori, Hossein Montakhab‐Yeganeh, Mehrak Zare
Parasite Immunology.2019;[Epub] CrossRef
In-depth comparison of cell-based methodological approaches to determine drug susceptibility of visceral Leishmania isolates
Sarah Hendrickx, Lieselotte Van Bockstal, Guy Caljon, Louis Maes, Kiyoshi Kita
PLOS Neglected Tropical Diseases.2019; 13(12): e0007885. CrossRef
Assessment of Leishmania cell lines expressing high levels of beta-galactosidase as alternative tools for the evaluation of anti-leishmanial drug activity
Aline C. da Silva Santos, Danielle M.N. Moura, Thiago A.R. dos Santos, Osvaldo P. de Melo Neto, Valéria R.A. Pereira
Journal of Microbiological Methods.2019; 166: 105732. CrossRef
Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays
Sarah L. Berry, Hamza Hameed, Anna Thomason, Marissa L. Maciej-Hulme, Somaia Saif Abou-Akkada, Paul Horrocks, Helen P. Price, Timothy G. Geary
PLOS Neglected Tropical Diseases.2018; 12(7): e0006639. CrossRef
Arginase activity in pathogenic and non-pathogenic species of Leishmania parasites
Alireza Badirzadeh, Tahereh Taheri, Yasaman Taslimi, Zahra Abdossamadi, Maryam Heidari-Kharaji, Elham Gholami, Baharehsadat Sedaghat, Maryam Niyyati, Sima Rafati, Armando Jardim
PLOS Neglected Tropical Diseases.2017; 11(7): e0005774. CrossRef
Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice
Zahra Abdossamadi, Negar Seyed, Farnaz Zahedifard, Tahereh Taheri, Yasaman Taslimi, Hossein Montakhab-Yeganeh, Alireza Badirzadeh, Mohammad Vasei, Safoora Gharibzadeh, Sima Rafati, Michael P. Pollastri
PLOS Neglected Tropical Diseases.2017; 11(12): e0006123. CrossRef
EGFP reporter protein: its immunogenicity in Leishmania-infected BALB/c mice
Samira Seif, Fereshteh Kazemi, Elham Gholami, Negar Seyed, Yasaman Taslimi, Sima Habibzadeh, Bahareh Azarian, Shahram Jamshidi, Mehrdad Hashemi, Sima Rafati, Tahereh Taheri
Applied Microbiology and Biotechnology.2016; 100(9): 3923. CrossRef

11,370 View
113 Download
15 Web of Science
Crossref

An Epidemiological Study of Cutaneous Leishmaniasis in Al-Jabal Al-Gharbi, Libya: Manal Z. M. Abdellatif, Khamis El-Mabrouk, Ashraf A. Ewis; Korean J Parasitol 2013;51(1):75-84.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.75

Abstract
PDF

Cutaneous leishmaniasis (CL) is an endemic parasitic infection in the Mediterranean region, including Libya and its Al-jabal Al-gharbi province. We aimed at studying the occupational relevance as well as other epidemiological aspects of CL. We investigated 140 CL cases who attended at Gharyan outpatient polyclinic during a period of 6 months in 2009. CL infection was clinically diagnosed and confirmed by demonstration of Leishmania parasites on smears from lesions. Our findings showed that males were more affected than females (P=0.04), and people above 10-years were more affected than younger ones (P=0.0001). A significant percent of CL cases belonged to Al-Kawasem subprovince (P=0.0001). Farm-related activities were the most frequent occupations among CL cases (P=0.04). In addition to farm workers, housewives and students are at risk groups since they are engaged at farm activities. Moreover, those who have occupations that require staying outdoors for a part of night, e.g., policemen, are also at risk. Compared to children, adult CL patients had multiple lesions (P=0.001) that were more prevalent in their upper and lower extremities than the face (P=0.0001). We conclude that CL is a major health problem in Al-jabal Al-gharbi province of Libya. The presence of rodents and sandflies makes it a suitable environment for Leishmania to spread in an endemic epidemiological pattern. Being engaged in farming activities or outdoor occupations increases the risk of infection. Various clinical patterns of CL suggest the presence of more than 1 species of Leishmania at Al-jabal Al-gharbi province. We propose that the 2 species responsible for CL in this area are L. major and L. tropica. Further investigations to identify the leishmanial species responsible for CL at Al-jabal Al-gharbi together with adoption of preventive and control programs are needed.

Citations

Citations to this article as recorded by

Ongoing presence of Leishmania major cutaneous leishmaniasis in Mauritania, 2016–2024
Aminetou El Moctar, Mohamed Ouldabdallahi Moukah, Abdoulaye Kassoum Koné, Mona Saout, Magalie Demar, Mamadou Dramani Fofana, Taleb Khyar Cheikh Mohamed Vadel, Mariem Kébé, Mahamadou Ali Thera, Romain Blaizot, Ali Ould Mohamed Salem Boukhary
BMC Infectious Diseases.2025;[Epub] CrossRef
A Retrospective Analysis of Human Cutaneous Leishmaniasis Epidemiology During 2016 - 2021 in Hoveyzeh County, Khuzestan Province, Iran
Mehdi Kian, Naser Hatamzadeh, Hamid Kassiri, Abas Naboureh, Zahra Makiani, Somayeh Mirzavand
Jundishapur Journal of Health Sciences.2024;[Epub] CrossRef
Cutaenous Leishmaniasis In Libya
Tarek Mohamed Arshah
Arab Board Medical Journal.2023; 24(1): 3. CrossRef
Human Cutaneous Leishmaniasis in North Africa and Its Threats to Public Health: A Statistical Study Focused on Djelfa (Algeria)
Fatma Messaoudene, Slimane Boukraa, Said Chaouki Boubidi, Ahlem Guerzou, Abdeldjalil Ouahabi
Microorganisms.2023; 11(10): 2608. CrossRef
Cutaneous Leishmaniasis in Al-Jabal Al-Gharbi, Libya: Incidence and Epidemiology
Abdalsalam Ashour, Ahmed Atia, Nisreen Akash, Elham Jumaa, Amira Alkhishrabi
Khalij-Libya Journal of Dental and Medical Research.2022; : 81. CrossRef
Spatiotemporal patterns of cutaneous leishmaniasis in the district upper and lower Dir, Khyber Pakhtunkhwa, Pakistan: A GIS-based spatial approaches
Ismail Zeb, Naveeda Akhter Qureshi, Nargis Shaheen, Mazhar Iqbal Zafar, Abid Ali, Aneeqa Hamid, Syed Aizaz Ali Shah, Asma Ashraf
Acta Tropica.2021; 217: 105861. CrossRef
Epidemiological and genetic characteristics of asymptomatic canine leishmaniasis and implications for human Leishmania infections in Egypt
Mai Abuowarda, Huda O. AbuBakr, Elshaimaa Ismael, Mohamed Shaalan, Mona A. Mohamed, Samira H. Aljuaydi
Zoonoses and Public Health.2021; 68(5): 413. CrossRef
Phylogenetic analysis of kinetoplast DNA: kDNA of Leishmania tropica in Thi-Qar province, Iraq
Mohammed Hassan Flaih, Fadhil Abbas Al-Abady, Khwam Reissan Hussein
Comparative Immunology, Microbiology and Infectious Diseases.2021; 78: 101696. CrossRef
Epidemiological situation analysis of cutaneous leishmaniasis in Batna (northeast): An important focus in Algeria
Nacer Eddine Messahel, Ismail Lafri, Idir Moualek, Karim Houali, Ahcène Hakem
Veterinary Parasitology: Regional Studies and Reports.2021; 26: 100621. CrossRef
Socioeconomic, demographic and landscape factors associated with cutaneous leishmaniasis in Kurunegala District, Sri Lanka
Tharaka Wijerathna, Nayana Gunathilaka, Kithsiri Gunawardena, Wasana Rodrigo
Parasites & Vectors.2020;[Epub] CrossRef
Epidemiology, Associated Factors and Treatment Methods of Cutaneous Leishmaniasis Based on Previous Data from 2013 to 2018 in Ilam, Western Iran
Nasrin Rezaee, Vahid Raissi, Ahmad Rajaeipour, Mehdi Nazari, Muhammad Getso, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim
Acta Parasitologica.2020; 65(3): 760. CrossRef
Clinico-epidemiological features of cutaneous leishmaniasis in Mazandaran Province, northern Iran
Mahmood Moosazadeh, Mahdi Afshari, Mohammadreza Parsaee, Ali charkameh, Asghar Nezammahalleh, Ghasem Abedi, Mahdi Fakhar, Seif Ali Mahdavi, Jalil Shojaei, Ahmad Ali Enayati
Clinical Epidemiology and Global Health.2019; 7(3): 378. CrossRef
Spatial epidemiology of cutaneous leishmaniasis in Colombia: socioeconomic and demographic factors associated with a growing epidemic
Andrés M Hernández, Juan D Gutierrez, Yanyu Xiao, Adam J Branscum, Diego F Cuadros
Transactions of The Royal Society of Tropical Medicine and Hygiene.2019; 113(9): 560. CrossRef
Visceral and Cutaneous Leishmaniases in a City in Syria and the Effects of the Syrian Conflict
Alexey Youssef, Rim Harfouch, Said El Zein, Zuheir Alshehabi, Rafea Shaaban, Souha S. Kanj
The American Journal of Tropical Medicine and Hygiene.2019; 101(1): 108. CrossRef
Clinical and epidemiological characteristics of cutaneous leishmaniasis in Sri Lanka
Devika Iddawela, Sanura Malinda Pallegoda Vithana, Dhilma Atapattu, Lanka Wijekoon
BMC Infectious Diseases.2018;[Epub] CrossRef
Epidemiological Aspects of Cutaneous Leishmaniasis during 2009-2016 in Kashan City, Central Iran
Doroodgar Moein, Doroodgar Masoud, Mahboobi Saeed, Doroodgar Abbas
The Korean Journal of Parasitology.2018; 56(1): 21. CrossRef
Cutaneous leishmaniasis in Qasr-e Shirin, a border area in the west of Iran
Yazdan Hamzavi, Naser Nazari, Nahid Khademi, Keivan Hassani, Arezoo Bozorgomid
Veterinary World.2018; : 1692. CrossRef
Entomological studies of phlebotomine sand flies (Diptera: Psychodidae) in relation to cutaneous leishmaniasis transmission in Al Rabta, North West of Libya
Mostafa Ramahdan Dokhan, Mohamed Amin Kenawy, Said Abdallah Doha, Shabaan Said El-Hosary, Taher Shaibi, Badereddin Bashir Annajar
Acta Tropica.2016; 154: 95. CrossRef
Genetic diversity of Leishmania tropica strains isolated from clinical forms of cutaneous leishmaniasis in rural districts of Herat province, Western Afghanistan, based on ITS1-rDNA
Mahdi Fakhar, Hossein Pazoki Ghohe, Sayed Abobakar Rasooli, Mehdi Karamian, Abdul Satar Mohib, Hajar Ziaei Hezarjaribi, Abdol Sattar Pagheh, Mohammad Amin Ghatee
Infection, Genetics and Evolution.2016; 41: 120. CrossRef
Geographical Distribution and New Situation ofLeishmaniaSpecies after the Control of Cutaneous Leishmaniasis Foci in Errachidia Province, Morocco, in 2014
El Alem Mohamed Mahmoud, Sebti Faiza, Med Lemine, Chichaoui Smaine, El Bachir Adlaoui, Habbari Khalid, Sadak Abderrahim, Fellah Hajiba
BioMed Research International.2016; 2016: 1. CrossRef
RISK FACTORS ASSOCIATED WITH AMERICAN CUTANEOUS LEISHMANIASIS IN AN ENDEMIC AREA OF BRAZIL
Alberon Ribeiro de ARAUJO, Nairomberg Cavalcanti PORTELA, Ana Paula Sampaio FEITOSA, Otamires Alves da SILVA, Ricardo Andrade Arraes XIMENES, Luiz Carlos ALVES, Fábio André BRAYNER
Revista do Instituto de Medicina Tropical de São Paulo.2016;[Epub] CrossRef
A Comparison of Demographic and Clinical Characteristics of Syrian and Turkish Patients with Cutaneous Leishmaniasis
Enver Turan, Nurittin Ardic, Hacer Altun Sürücü, Mustafa Aksoy, Abhay R. Satoskar, Sanjay Varikuti, Nebiye Doni, Steve Oghumu, Abdullah Yesilova, Yavuz Yeşilova
The American Journal of Tropical Medicine and Hygiene.2015; 93(3): 559. CrossRef
Leishmaniases in Maghreb: An endemic neglected disease
Dhekra Chaara, Najoua Haouas, Jean Pierre Dedet, Hamouda Babba, Francine Pratlong
Acta Tropica.2014; 132: 80. CrossRef

12,133 View
123 Download
Crossref

The Route of Leishmania tropica Infection Determines Disease Outcome and Protection against Leishmania major in BALB/c Mice: Hamid Mahmoudzadeh-Niknam, Ghader Khalili, Firoozeh Abrishami, Ali Najafy, Vahid Khaze; Korean J Parasitol 2013;51(1):69-74.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.69

Abstract
PDF

Leishmania tropica is one of the causative agents of leishmaniasis in humans. Routes of infection have been reported to be an important variable for some species of Leishmania parasites. The role of this variable is not clear for L. tropica infection. The aim of this study was to explore the effects of route of L. tropica infection on the disease outcome and immunologic parameters in BALB/c mice. Two routes were used; subcutaneous in the footpad and intradermal in the ear. Mice were challenged by Leishmani major, after establishment of the L. tropica infection, to evaluate the level of protective immunity. Immune responses were assayed at week 1 and week 4 after challenge. The subcutaneous route in the footpad in comparison to the intradermal route in the ear induced significantly more protective immunity against L. major challenge, including higher delayed-type hypersensitivity responses, more rapid lesion resolution, lower parasite loads, and lower levels of IL-10. Our data showed that the route of infection in BALB/c model of L. tropica infection is an important variable and should be considered in developing an appropriate experimental model for L. tropica infections.

Citations

Citations to this article as recorded by

The Immune Memory Response of In Vitro-Polarised Th1, Th2, and Th17 Cells in the Face of Ovalbumin-Transgenic Leishmania major in a Mouse Model
Mebrahtu G. Tedla, Musammat F. Nahar, Alison L. Every, Jean-Pierre Y. Scheerlinck
International Journal of Molecular Sciences.2024; 25(16): 8753. CrossRef
Interleukin-4 Responsive Dendritic Cells Are Dispensable to Host Resistance Against Leishmania mexicana Infection
Bernard Ong’ondo Osero, Zama Cele, Raphael Taiwo Aruleba, Rebeng A. Maine, Mumin Ozturk, Manfred B. Lutz, Frank Brombacher, Ramona Hurdayal
Frontiers in Immunology.2022;[Epub] CrossRef
A Mouse Model of Ulcerative Cutaneous Leishmaniasis by Leishmania (Viannia) panamensis to Investigate Infection, Pathogenesis, Immunity, and Therapeutics
Natalia Muñoz-Durango, Alexander Gómez, Natalia García-Valencia, Miguel Roldán, Marcela Ochoa, David E. Bautista-Erazo, José R. Ramírez-Pineda
Frontiers in Microbiology.2022;[Epub] CrossRef
The outcome of arginase activity inhibition in BALB/c mice hosting Leishmania tropica
Shima Nahidi, Elham Gholami, Yasaman Taslimi, Sima Habibzadeh, Negar Seyed, Elaheh Davarpanah, Alireza Ghanadan, Sima Rafati, Tahereh Taheri
Parasite Immunology.2020;[Epub] CrossRef
To the Skin and Beyond: The Immune Response to African Trypanosomes as They Enter and Exit the Vertebrate Host
Omar A. Alfituri, Juan F. Quintana, Annette MacLeod, Paul Garside, Robert A. Benson, James M. Brewer, Neil A. Mabbott, Liam J. Morrison, Paul Capewell
Frontiers in Immunology.2020;[Epub] CrossRef
DermotropicLeishmania donovaniin Sri Lanka: visceralizing potential in clinical and preclinical studies
K. K. G. D. U. L. KARIYAWASAM, A. SELVAPANDIYAN, H. V. Y. D. SIRIWARDANA, A. DUBE, P. KARUNANAYAKE, S. A. S. C. SENANAYAKE, R. DEY, S. GANNAVARAM, H. L. NAKHASI, N. D. KARUNAWEERA
Parasitology.2018; 145(4): 443. CrossRef
Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Mosayeb Rostamian, Fariborz Bahrami, Hamid M. Niknam, Humberto Lanz-Mendoza
PLOS ONE.2018; 13(9): e0204491. CrossRef
Route of Infection Affects Pathogenicity of Leishmania major in BALB/c Mice
Ehsan Sarreshteh, Mosayeb Rostamian, Mahsa Tat Asadi, Firoozeh Abrishami, Ali Najafi, Maryam Abolghazi, Hamid Mahmoudzadeh Niknam
Journal of Medical Microbiology and Infectious Diseases .2017; 5(1): 26. CrossRef
Indigenous microbiota and Leishmaniasis
M. E. M. Lopes, M. B. H. Carneiro, L. M. dos Santos, L. Q. Vieira
Parasite Immunology.2016; 38(1): 37. CrossRef
Cross‐protective efficacy from a immunogen firstly identified in Leishmania infantum against tegumentary leishmaniasis
V. T. Martins, D. P. Lage, M. C. Duarte, L. E. Costa, M. A. Chávez‐Fumagalli, B. M. Roatt, D. Menezes‐Souza, C. A. P. Tavares, E. A. F. Coelho
Parasite Immunology.2016; 38(2): 108. CrossRef
Coinjection with TLR2 Agonist Pam3CSK4 Reduces the Pathology of Leishmanization in Mice
Lu Huang, Meleana Hinchman, Susana Mendez, Mary Ann McDowell
PLOS Neglected Tropical Diseases.2015; 9(3): e0003546. CrossRef
Site-Dependent Recruitment of Inflammatory Cells Determines the Effective Dose of Leishmania major
Flavia L. Ribeiro-Gomes, Eric Henrique Roma, Matheus B. H. Carneiro, Nicole A. Doria, David L. Sacks, Nathan C. Peters, J. A. Appleton
Infection and Immunity.2014; 82(7): 2713. CrossRef

9,114 View
84 Download
Crossref

Miltefosine-Induced Apoptotic Cell Death on Leishmania major and L. tropica Strains: Shahram Khademvatan, Mohammad Javad Gharavi, Fakher Rahim, Jasem Saki; Korean J Parasitol 2011;49(1):17-23.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.17

Abstract
PDF

The aim of this study was to assess the cytotoxic effects of various concentrations of miltefosine on Leishmania major (MRHO/IR/75/ER) and L. tropica (MHOM/IR/02/Mash10) promastigotes and to observe the programmed cell death features. The colorimetric MTT assay was used to find L. major and L. tropica viability and the obtained results were expressed as 50% inhibitory concentration (IC50). Also, 50% effective doses (ED50) for L. major and L. tropica amastigotes were also determined. Annexin-V FLUOS staining was performed to study the cell death properties of miltefosine using FACS analysis. Qualitative analysis of the total genomic DNA fragmentation was performed by agarose gel electrophoresis. Furthermore, to observe changes in cell morphology, promastigotes were examined using light microscopy. In both strains of L. major and L. tropica, miltefosine induced dose-dependent death with features of apoptosis, including cell shrinkage, DNA laddering, and externalization of phosphatidylserine. The IC50 was achieved at 22 ?M and 11 ?M for L. major and L. tropica after 48 hr of incubation, respectively. ED50 of L. major and L. tropica amastigotes were 5.7 ?M and 4.2 ?M, respectively. Our results indicate that miltefosine induces apoptosis of the causative agent of cutaneous leishmaniasis in a dose-dependent manner. Interestingly, L. major did not display any apoptotic changes when it was exposed to miltefosine in concentrations sufficient to kill L. tropica.

Citations

Citations to this article as recorded by

Miltefosine reduces coxsackievirus B3 lethality of mice with enhanced STAT3 activation
Chun Yu Zhang, Cheng-Huei Hung, Yi-Ling Hsiao, Tung-Miao Chang, Yu-Chieh Su, Li-Chiu Wang, Shih-Min Wang, Shun-Hua Chen
Antiviral Research.2024; 223: 105824. CrossRef
Appraisal of Chitosan-Coated Lipid Nano-Combination with Miltefosine and Albendazole in the Treatment of Murine Trichinellosis: Experimental Study with Evaluation of Immunological and Immunohistochemical Parameters
Asmaa F. Ibrahim, Sahar M. Selim, Dalia A. Shafey, Dina M. Sweed, Shaimaa A. Farag, Marwa A. Gouda
Acta Parasitologica.2024; 69(1): 929. CrossRef
Successful treatment of complex cutaneous leishmaniasis (L. tropica) in a 3‐year‐old Syrian boy with miltefosine
Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153. CrossRef
Erfolgreiche Therapie einer komplexen kutanen Leishmaniose (L. tropica) bei einem 3‐jährigen syrischen Jungen mit Miltefosin
Linda Golle, Cord Sunderkötter, Jovine Ehrenreich, Johannes Wohlrab
JDDG: Journal der Deutschen Dermatologischen Gesellschaft.2024; 22(8): 1153. CrossRef
Miltefosine induces reproductive toxicity during sperm capacitation by altering PI3K/AKT signaling pathway
Eun-Ju Jung, Woo-Jin Lee, Jeong-Won Bae, Woo-Sung Kwon
Environmental Toxicology and Pharmacology.2024; 111: 104565. CrossRef
Anti-leishmanial activity of Hypericum Scabrum extract against Leishmania major
Reza Saberi, Zaynab Jamshidzad, Elaheh Karimi, Jahangir Abdi, Razi Naserifar, Asad Mirzaei
AMB Express.2024;[Epub] CrossRef
A short-term method to evaluate anti-leishmania drugs by inhibition of stage differentiation in Leishmania mexicana using flow cytometry
Christian Florian Teh-Poot, Victor Manuel Dzul-Huchim, Jonathan M. Mercado, Liliana Estefanía Villanueva-Lizama, Maria Elena Bottazzi, Kathryn M. Jones, Francis T.F. Tsai, Julio Vladimir Cruz-Chan
Experimental Parasitology.2023; 249: 108519. CrossRef
The Antileishmanial Activity of Eugenol Associated with Lipid Storage Reduction Rather Than Membrane Properties Alterations
Kristelle Hughes, Thanh Binh Le, Patrick Van Der Smissen, Donatienne Tyteca, Marie-Paule Mingeot-Leclercq, Joëlle Quetin-Leclercq
Molecules.2023; 28(9): 3871. CrossRef
Laurequinone, a Lead Compound against Leishmania
Sara García-Davis, Atteneri López-Arencibia, Carlos J. Bethencourt-Estrella, Desirée San Nicolás-Hernández, Ezequiel Viveros-Valdez, Ana R. Díaz-Marrero, José J. Fernández, Jacob Lorenzo-Morales, José E. Piñero
Marine Drugs.2023; 21(6): 333. CrossRef
In vitro efficacy of polymer coated miltefosine drug against leishmania tropica
Mehvish Khokhar, Muhammad Adnan Shereen, Momin Khan, Rahat Ullah Khan, Aamir Sohail, Imdad Ullah Khan, Inam Ullah Khan, Saadullah Khattak
Journal of Parasitic Diseases.2022; 46(2): 366. CrossRef
Tackling Drug Resistance and Other Causes of Treatment Failure in Leishmaniasis
Gert-Jan Wijnant, Franck Dumetz, Laura Dirkx, Dimitri Bulté, Bart Cuypers, Katrien Van Bocxlaer, Sarah Hendrickx
Frontiers in Tropical Diseases.2022;[Epub] CrossRef
The Effect of Curcumin on the Expression of INFγ, TNF-α, and iNOS Genes in PBMCs Infected with Leishmania major [MRHO/IR/75/ER]
Soheila Alinejad, Shahram Khademvatan, Shahla Amani, Negar Asadi, Khosrow Hazrati Tappeh, Elham Yousefi, Touraj Miandoabi
Infectious Disorders - Drug Targets.2022;[Epub] CrossRef
In Vitro Study of Cytotoxic Mechanisms of Alkylphospholipids and Alkyltriazoles in Acute Lymphoblastic Leukemia Models
Larissa de Oliveira Passos Jesus, Aline Aparecida de Souza, Heron Fernandes Vieira Torquato, Vanessa Silva Gontijo, Rossimirian Pereira de Freitas, Tarsis Ferreira Gesteira, Vivien Jane Coulson-Thomas, Ricardo José Soares Torquato, Aparecida Sadae Tanaka,
Molecules.2022; 27(23): 8633. CrossRef
Anti-leishmanial physalins—Phytochemical investigation, in vitro evaluation against clinical and MIL-resistant L. tropica strains and in silico studies
Saira Bano, Memoona Bibi, Saba Farooq, Humaira Zafar, Muniza Shaikh, Behram Khan Khoso, Sammer Yousuf, M. Iqbal Choudhary, Mohammad Shahid
PLOS ONE.2022; 17(11): e0274543. CrossRef
Leishmanicidal Activity and Ultrastructural Changes of Maslinic Acid Isolated from Hyptidendron canum
Jéssica Adriana Jesus, Márcia Dalastra Laurenti, Matheus Lopes Silva, João Henrique Ghilardi Lago, Luiz Felipe Domingues Passero, Valeria Sülsen
Evidence-Based Complementary and Alternative Medicine.2021; 2021: 1. CrossRef
A Novel Automated Framework for QSAR Modeling of Highly Imbalanced Leishmania High-Throughput Screening Data
Omar Casanova-Alvarez, Aliuska Morales-Helguera, Miguel Ángel Cabrera-Pérez, Reinaldo Molina-Ruiz, Christophe Molina
Journal of Chemical Information and Modeling.2021; 61(7): 3213. CrossRef
Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
Jasem Saki, Khalil Saki, Reza Arjmand
Jundishapur Journal of Natural Pharmaceutical Products.2021;[Epub] CrossRef
Une leishmaniose cutanée réfractaire : intérêt de la miltéfosine
C. Guerveno, K. Delavigne, A. Berry, G. Martin-Blondel, P. Delobel
Médecine et Maladies Infectieuses.2019; 49(4): 281. CrossRef
Natural compounds from plants controlling leishmanial growth via DNA damage and inhibiting trypanothione reductase and trypanothione synthetase: an in vitro and in silico approach
Shaila Mehwish, Huma Khan, Ashfaq Ur Rehman, Asif Ullah Khan, Mubarak Ali Khan, Obaid Hayat, Mansoor Ahmad, Abdul Wadood, Nazif Ullah
3 Biotech.2019;[Epub] CrossRef
Antileishmanial and cytotoxic activities of ionic surfactants compared to those of miltefosine
Lais Alonso, Éder Jeferson Souza Cardoso, Rodrigo Saar Gomes, Sebastião Antônio Mendanha, Miriam Leandro Dorta, Antonio Alonso
Colloids and Surfaces B: Biointerfaces.2019; 183: 110421. CrossRef
In vitro study of the efficacy of Solanum nigrum against Leishmania major
Christine N. Mutoro, Johnson K. Kinyua, Joseph K. Ng'ang'a, Daniel W. Kariuki, Johnstone M. Ingonga, Christopher O. Anjili
F1000Research.2018; 7: 1329. CrossRef
The cytotoxic activity of miltefosine against Leishmania and macrophages is associated with dynamic changes in plasma membrane proteins
Kelly Souza Fernandes, Paulo Eduardo Narcizo de Souza, Miriam Leandro Dorta, Antonio Alonso
Biochimica et Biophysica Acta (BBA) - Biomembranes.2017; 1859(1): 1. CrossRef
Antileishmanial and Immunomodulatory Activity of Allium sativum (Garlic)
Masoud Foroutan-Rad, Khosrow Hazrati Tappeh, Shahram Khademvatan
Journal of Evidence-Based Complementary & Alternative Medicine.2017; 22(1): 141. CrossRef
Effect of 1,2,3-triazole salts, non-classical bioisosteres of miltefosine, on Leishmania amazonensis
Pedro H.F. Stroppa, Luciana M.R. Antinarelli, Arturene M.L. Carmo, Jacy Gameiro, Elaine S. Coimbra, Adilson D. da Silva
Bioorganic & Medicinal Chemistry.2017; 25(12): 3034. CrossRef
Pomegranate (Punica granatum) Juice Shows Antioxidant Activity against Cutaneous Leishmaniasis-Induced Oxidative Stress in Female BALB/c Mice
Badriah Alkathiri, Manal El-Khadragy, Dina Metwally, Ebtesam Al-Olayan, Muhammed Bakhrebah, Ahmed Abdel Moneim
International Journal of Environmental Research and Public Health.2017; 14(12): 1592. CrossRef
Enterocin AS-48 as Evidence for the Use of Bacteriocins as New Leishmanicidal Agents
María Ángeles Abengózar, Rubén Cebrián, José María Saugar, Teresa Gárate, Eva Valdivia, Manuel Martínez-Bueno, Mercedes Maqueda, Luis Rivas
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Cytotoxic Activity ofHolothuria leucospilotaExtract againstLeishmania infantum In Vitro
Shahram Khademvatan, Alborz Eskandari, Jasem Saki, Masoud Foroutan-Rad
Advances in Pharmacological Sciences.2016; 2016: 1. CrossRef
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
Adriana Bezerra-Souza, Eduardo S. Yamamoto, Márcia D. Laurenti, Susan P. Ribeiro, Luiz Felipe D. Passero
Parasitology International.2016; 65(6): 702. CrossRef
New Approaches to Overcome Transport Related Drug Resistance in Trypanosomatid Parasites
Jose A. Garcia-Salcedo, Juan D. Unciti-Broceta, Javier Valverde-Pozo, Miguel Soriano
Frontiers in Pharmacology.2016;[Epub] CrossRef
Biological Activity of Coumarin Derivatives as Anti-Leishmanial Agents
Vineetha Mandlik, Sohan Patil, Ramanamurthy Bopanna, Sudipta Basu, Shailza Singh, Imtaiyaz Hassan
PLOS ONE.2016; 11(10): e0164585. CrossRef
Molecular docking and molecular dynamics simulation study of inositol phosphorylceramide synthase – inhibitor complex in leishmaniasis: Insight into the structure based drug design
Vineetha Mandlik, Shailza Singh
F1000Research.2016; 5: 1610. CrossRef
A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum
Paloma Tejera Nevado, Eugenia Bifeld, Katharina Höhn, Joachim Clos
Antimicrobial Agents and Chemotherapy.2016; 60(9): 5262. CrossRef
Molecular docking and molecular dynamics simulation study of inositol phosphorylceramide synthase – inhibitor complex in leishmaniasis: Insight into the structure based drug design
Vineetha Mandlik, Shailza Singh
F1000Research.2016; 5: 1610. CrossRef
Cinnamic Acid Bornyl Ester Derivatives from Valeriana wallichii Exhibit Antileishmanial In Vivo Activity in Leishmania major-Infected BALB/c Mice
Anita Masic, Ana Maria Valencia Hernandez, Sudipta Hazra, Jan Glaser, Ulrike Holzgrabe, Banasri Hazra, Uta Schurigt, Bhaskar Saha
PLOS ONE.2015; 10(11): e0142386. CrossRef
Evaluation of the effect of miltefosine on Trichomonas vaginalis
Débora Afonso Silva Rocha, Ivone de Andrade Rosa, Wanderley de Souza, Marlene Benchimol
Parasitology Research.2014; 113(3): 1041. CrossRef
In silico and in vitro comparative activity of novel experimental derivatives against Leishmania major and Leishmania infantum promastigotes
Shahram Khademvatan, Neda Adibpour, Alborz Eskandari, Saeed Rezaee, Mahmoud Hashemitabar, Fakher Rahim
Experimental Parasitology.2013; 135(2): 208. CrossRef
Pistagremic acid a new leishmanicidal triterpene isolated from Pistacia integerrima Stewart
Ghias Uddin, Abdur Rauf, Mohammad Arfan, Waliullah, Inamullah khan, Mumtaz Ali, Mazhar Taimur, Inaayat ur-Rehman, Samiullah
Journal of Enzyme Inhibition and Medicinal Chemistry.2012; 27(5): 646. CrossRef

12,527 View
99 Download
Crossref

Anti-leishmanial Effects of Trinitroglycerin in BALB/C Mice Infected with Leishmania major via Nitric Oxide Pathway: Hossein Nahrevanian, Mana Najafzadeh, Reza Hajihosseini, Habib Nazem, Mahin Farahmand, Zahra Zamani; Korean J Parasitol 2009;47(2):109-115.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.109

Abstract
PDF

This study investigated whether trinitroglycerine (TNG) as nitric oxide (NO) releasing agent had anti-leishmanial effects and mediated pathology in BALB/c mice infected with Leishmania major. Cutaneous leishmaniasis (CL), a zoonotic infection caused by leishmania protozoa is still one of the health problems in the world and in Iran. NO is involved in host immune responses against intracellular L. major, and leishmania killing by macrophages is mediated by this substance. Moreover, application of CL treatment with NO-donors has been recently indicated. In our study, TNG was used for its ability to increase NO and to modify CL infection in mice, in order to evaluate NO effects on lesion size and formation, parasite proliferation inside macrophages, amastigote visceralization in target organs, and NO induction in plasma and organ suspensions. Data obtained in this study indicated that TNG increased plasma and liver-NO, reduced lesion sizes, removed amastigotes from lesions, livers, spleens, and lymph nodes, declined proliferation of amastigotes, hepatomegaly, and increased survival rate. However, TNG reduced spleen-NO and had no significant effects on spelenomegaly. The results show that TNG therapy reduced leishmaniasis and pathology in association with raised NO levels. TNG had some antiparasitic activity by reduction of positive smears from lesions, livers, spleens, and lymph nodes, which could emphasize the role of TNG to inhibit visceralization of L. major in target organs.

Citations

Citations to this article as recorded by

Topical liposomal amphotericin B gel treatment for cutaneous leishmaniasis caused by Leishmania major: a double‐blind, randomized, placebo‐controlled, pilot study
Amir Horev, Orli Sagi, Eyal Zur, Shalom Ben‐Shimol
International Journal of Dermatology.2023; 62(1): 40. CrossRef
Partial Immunotherapy of Leishmaniasis by in vivo Trial of L-Arginine in Balb/c Mice Infected with Leishmania major via Nitric Oxide Pathway
Fatemeh Faezi, Hossein Nahrevania, Mahin Farahmand, Mohammad Sayyah, Seyed Kazem Bidoki, Sara Nemati
International Journal of Biological Chemistry.2015; 9(3): 110. CrossRef
Is topical nitric oxide and cryotherapy more effective than cryotherapy in the treatment of old world cutaneous leishmaniasis?
Farideh Jowkar, Farideh Dehghani, Akram Jamshidzadeh
Journal of Dermatological Treatment.2012; 23(2): 131. CrossRef
Immunomodulation by chemotherapeutic agents against Leishmaniasis
Piu Saha, Debanjan Mukhopadhyay, Mitali Chatterjee
International Immunopharmacology.2011; 11(11): 1668. CrossRef
Leishmania–macrophage interactions: Insights into the redox biology
Tim Van Assche, Maartje Deschacht, Raquel A. Inocêncio da Luz, Louis Maes, Paul Cos
Free Radical Biology and Medicine.2011; 51(2): 337. CrossRef
Nitric oxide contributes to learning and memory deficits observed in hypothyroid rats during neonatal and juvenile growth
Mahmoud Hosseini, Samaneh Sadat Dastghaib, Houshang Rafatpanah, Mosa Al-Reza Hadjzadeh, Hossein Nahrevanian, Ismaeil Farrokhi
Clinics.2010; 65(11): 1175. CrossRef

9,398 View
120 Download
Crossref

Brief Communication

Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes: Majid Zeinali, Sussan K. Ardestani, Amina Kariminia; Korean J Parasitol 2007;45(4):287-293.
Published online December 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.4.287

Abstract
PDF

The identification and characterization of antigens that elicit human T cell responses is an important step toward understanding of Leishmania major infection and ultimately in the development of a vaccine. Micropreparative SDS-PAGE followed by electrotransfer to a PVDF membrane and elution of proteins from the PVDF, was used to separate 2 novel proteins from L. major promastigotes, which can induce antibodies of the IgG2a isotype in mice and also are recognized by antisera of recovered human cutaneous leishmaniasis subjects. Fractionation of the crude extract of L. major revealed that all detectable proteins of interest were present within the soluble Leishmania antigens (SLA). Quantitation of these proteins showed that their expression in promastigotes is relatively very low. Considering the molecular weight, immunoreactivity, chromatographic and electrophoretic behavior in reducing and non-reducing conditions, these proteins are probably 2 isoforms of a single protein. A digest of these proteins was resolved on Tricine-SDS-PAGE and immunoreactive fragments were identified by human sera. Two immunoreactive fragments (36.4 and 34.8 kDa) were only generated by endoproteinase Glu-C treatment. These immunoreactive fragments or their parent molecules may be ideal candidates for incorporation in a cocktail vaccine against cutaneous leishmaniasis.

Citations

Citations to this article as recorded by

Highly Effective Serodiagnosis for Chagas' Disease
Pilar Hernández, Michael Heimann, Cristina Riera, Marco Solano, José Santalla, Alejandro O. Luquetti, Ewald Beck
Clinical and Vaccine Immunology.2010; 17(10): 1598. CrossRef

7,354 View
92 Download
Crossref

Original Articles

Leishmania tropica infection, in comparison to Leishmania major, induces lower delayed type hypersensitivity in BALB/c mice: Hamid Mahmoudzadeh-Niknam, Simin Sadat Kiaei, Davood Iravani; Korean J Parasitol 2007;45(2):103-109.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.103

Abstract
PDF

Leishmania tropica and L. major are etiologic agents of human cutaneous leishmaniasis. Delayed type hypersensitivity (DTH) is an immunologic response that has been frequently used as a correlate for protection against or sensitization to leishmania antigen. In BALB/c mice, L. tropica infection results in non-ulcerating disease, whereas L. major infection results in destructive lesions. In order to clarify the immunologic mechanisms of these 2 different outcomes, we compared the ability of these 2 leishmania species in induction of DTH response in this murine model. BALB/c mice were infected with L. major or L. tropica, and disease evolution and DTH responses were determined. The results show that the primary L. major infection can exacerbate the secondary L. major infection and is associated with DTH response. Higher doses of the primary L. major infection result in more disease exacerbation of the secondary L. major infection as well as higher DTH response. L. tropica infection induces lower DTH responses than L. major. We have previously reported that the primary L. tropica infection induces partial protection against the secondary L. major infection in BALB/c mice. Induction of lower DTH response by L. tropica suggests that the protection induced against L. major by prior L. tropica infection may be due to suppression of DTH response.

Citations

Citations to this article as recorded by

Expression of key cytokines in dog macrophages infected by Leishmania tarentolae opening new avenues for the protection against Leishmania infantum
Viviane Noll Louzada-Flores, Maria Stefania Latrofa, Jairo Alfonso Mendoza-Roldan, Maria Stella Lucente, Sara Epis, Ilaria Varotto-Boccazzi, Claudio Bandi, Domenico Otranto
Scientific Reports.2024;[Epub] CrossRef
Interspecies and Intrastrain Interplay among Leishmania spp. Parasites
Bruna Dias das Chagas, Thaís Martins Pereira, Lilian Motta Cantanhêde, Gabriela Pereira da Silva, Mariana Côrtes Boité, Luiza de Oliveira Ramos Pereira, Elisa Cupolillo
Microorganisms.2022; 10(10): 1883. CrossRef
Integration of Bioinformatics and in vitro Analysis Reveal Anti-leishmanial Effects of Azithromycin and Nystatin
Irum Jehangir, Syed Farhan Ahmad, Maryam Jehangir, Anwar Jamal, Momin Khan
Current Bioinformatics.2019; 14(5): 450. CrossRef
Vaccination with whole-cell killed or recombinant leishmanial protein and toll-like receptor agonists against Leishmania tropica in BALB/c mice
Mosayeb Rostamian, Fariborz Bahrami, Hamid M. Niknam, Humberto Lanz-Mendoza
PLOS ONE.2018; 13(9): e0204491. CrossRef
Route of Infection Affects Pathogenicity of Leishmania major in BALB/c Mice
Ehsan Sarreshteh, Mosayeb Rostamian, Mahsa Tat Asadi, Firoozeh Abrishami, Ali Najafi, Maryam Abolghazi, Hamid Mahmoudzadeh Niknam
Journal of Medical Microbiology and Infectious Diseases .2017; 5(1): 26. CrossRef
Studies on cocktails of 31‐kDa, 36‐kDa and 51‐kDa antigens of Leishmania donovani along with saponin against murine visceral leishmaniasis
H. Kaur, A. Thakur, S. Kaur
Parasite Immunology.2015; 37(4): 192. CrossRef
A Review: The Current In Vivo Models for the Discovery and Utility of New Anti-leishmanial Drugs Targeting Cutaneous Leishmaniasis
Emily Rose Mears, Farrokh Modabber, Robert Don, George E. Johnson, Louis Maes
PLOS Neglected Tropical Diseases.2015; 9(9): e0003889. CrossRef
Studies on the protective efficacy of second-generation vaccine along with standard antileishmanial drug in Leishmania donovani infected BALB/c mice
JYOTI JOSHI, SUKHBIR KAUR
Parasitology.2014; 141(4): 554. CrossRef
The Route of Leishmania tropica Infection Determines Disease Outcome and Protection against Leishmania major in BALB/c Mice
Hamid Mahmoudzadeh-Niknam, Ghader Khalili, Firoozeh Abrishami, Ali Najafy, Vahid Khaze
The Korean Journal of Parasitology.2013; 51(1): 69. CrossRef
Antineoplastic drug, carboplatin, protects mice against visceral leishmaniasis
Tejinder Kaur, Prerna Makkar, Kulbir Randhawa, Sukhbir Kaur
Parasitology Research.2013; 112(1): 91. CrossRef
Protective immunity against Leishmania major induced by Leishmania tropica infection of BALB/c mice
Hamid Mahmoudzadeh-Niknam, Simin Sadat Kiaei, Davood Iravani
Experimental Parasitology.2011; 127(2): 448. CrossRef
FML vaccine against canine visceral leishmaniasis: from second-generation to synthetic vaccine
Clarisa B Palatnik-de-Sousa, André de Figueiredo Barbosa, Sandra Maria Oliveira, Dirlei Nico, Robson Ronney Bernardo, Wania R Santos, Mauricio M Rodrigues, Irene Soares, Gulnara P Borja-Cabrera
Expert Review of Vaccines.2008; 7(6): 833. CrossRef
Viscerotropic growth pattern of Leishmania tropica in BALB/c mice is suggestive of a murine model for human viscerotropic leishmaniasis
Hamid Mahmoudzadeh-Niknam, Simin Sadat Kiaei, Davood Iravani
The Korean Journal of Parasitology.2007; 45(4): 247. CrossRef

8,667 View
94 Download
Crossref

Childhood cutaneous leishmaniasis: report of 117 cases from Iran: Safar Ali Talari, Rezvan Talaei, Gholamreza Shajari, Zarichehr Vakili, Abbas Taghaviardakani; Korean J Parasitol 2006;44(4):355-360.
Published online December 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.4.355

Abstract
PDF

Cutaneous leishmaniasis (CL), due to Leishmania major, is endemic in different parts of Iran and has long been recognized in most provinces of Iran. This study was conducted to determine the prevalence of childhood leishmaniasis in 3 areas at the southeast of Kashan. A descriptive study was carried out on all children referred to central laboratories during a 3-year period. Initial information including age, sex, sites of ulcer on the body, number of lesions, address, and the place of the disease was obtained. The study gathered 117 children, and the results showed a prevalence of 7.2% in patients with lesions among the population and 4.2% of people displayed lesion and scar. The ages of subjects were from 6 to 15 years (average 9.75 years). The boy: girl ratio was 1.2. All of our patients lived in an endemic area. The face was affected in 47.0% of cases. The encountered forms of leishmaniasis are as follows: papulonodular 27.4%, ulcer 60.7%, sporotrichoid 6%, impetiginous 2.5%, and erysipeloid 3.4%. Treatment with intramuscular meglumine antimoniate 20-30 mg/kg/day was done for 93 patients. Meglumine antimoniate treatment was tolerated with no side effects. All leishmaniasis lesions healed within an average period of 2-14 months. Hyperpigmented scars were formed in 25.6% of the patients, atrophic scars in 4.3%, and hypopigmented scars were in 3.4%, respectively. The findings of this study indicate increased prevalence of CL in the villages at the area of Kashan and Aran-Bidgol. The clinical finding patterns belonged to different endemic strains of L. major in Isfahan, which indicates the possible transmission of infection from Isfahan to this area.

Citations

Citations to this article as recorded by

Molecular diagnosis and epidemiological aspects of cutaneous Leishmaniasis in Aleppo: Current status
Lana Kourieh, Mohammad Y. Abajy, Mahasen Alkebajy, Silva Ishkhanian, Ream Nayal
Parasite Epidemiology and Control.2025; 28: e00412. CrossRef
Epidemiological survey, molecular profiling and phylogenetic analysis of cutaneous leishmaniasis in Khyber Pakhtunkhwa, Pakistan
Wasia Ullah, Adil Khan, Sadaf Niaz, Maged A Al-Garadi, Nasreen Nasreen, Ayman A Swelum, Mourad Ben Said
Transactions of The Royal Society of Tropical Medicine and Hygiene.2024; 118(4): 273. CrossRef
Epidemiology and clinical features of parasitic disease Leishmaniasis. A case study from Pakistan
Muhaibullah khan, Sheraz Ahmad, Hamayun saeed, M. Junaid Akhtar, Amna Munir
Medical Reports.2024; 6: 100090. CrossRef
Effect of pentavalent antimony compounds on the inflammatory, hematological and biochemical parameters in patients with cutaneous leishmaniasis
Kadir Kaya
Cutaneous and Ocular Toxicology.2024; 43(4): 305. CrossRef
Prevalence of cutaneous Leishmaniasis in the Middle East: a systematic review and meta-analysis
Mohsen Karami, Tahmineh Gorgani-Firouzjaee, Mohammad Chehrazi
Pathogens and Global Health.2023; 117(4): 356. CrossRef
Distribution, clinical features, and epidemiology of cutaneous leishmaniasis: A case study of District Haripur, KPK, Pakistan
Muhaibullah Khan, Shehzad Ghayyur, Samina yasmin
Medical Reports.2023; 2: 100025. CrossRef
Geographical distribution and molecular epidemiology of cutaneous leishmaniasis in Fars Province, southern Iran
Tahereh Davoodi, Ali Khamesipour, Saeed Shahabi, Fatemeh Gholizadeh, Daniel Pourkamal, Gholamreza Hatam
Environmental Science and Pollution Research.2022; 29(3): 4024. CrossRef
Epidemiological and pathological characteristics of Cutaneous Leishmaniasis from Baluchistan Province of Pakistan
Aisha Khan, Rawan Sajid, Shaista Gul, Ashiq Hussain, Mohammad Tahir Zehri, Shumaila Naz, Sami Simsek, Shahid Waseem, Muhammad Sohail Afzal, Syed Kamran Ul Hassan Naqvi, Muhammad Qasim, Haroon Ahmed
Parasitology.2021; 148(5): 591. CrossRef
The Effects of Some Climate Factors upon Frequency Distribution of Local Sandflies Breed in Misan Province, Iraq
Rasha K A Alsaad, May H Kawan
The Iraqi Journal of Veterinary Medicine.2021; 45(1): 63. CrossRef
Pediatric Cutaneous Leishmaniasis in Hormozgan Province, Southeast Iran during 2016-2020: A Descriptive Epidemiological Study
Sajjad Fekrijaski, Mehdi Zare, Moussa Soleimani-Ahmadi, Kaveh Soleimani, Alireza Sanei-Dehkordi
Journal of Occupational Health and Epidemiology.2021; 10(4): 224. CrossRef
Pediatric Cutaneous Leishmaniasis
Shagufta Rather, Atiya Yaseen, Faizan Younus Shah, Mashkoor Wani, Kewal Krishan, Sumaya Zirak, Rajesh Sharma, Iffat Hassan, Devraj Dogra, Ruby Rishi
Indian Dermatology Online Journal.2021; 12(6): 852. CrossRef
La leishmaniose cutanée de l’enfant au Maroc : particularités cliniques et épidémiologiques
B. Baghad, M. Riyad, R. Razanapinaritra, H. Maksouri, H. Ben Errais, S. Chiheb
Annales de Dermatologie et de Vénéréologie.2020; 147(2): 106. CrossRef
Molecular epidemiological survey of cutaneous leishmaniasis from Azad Jammu and Kashmir, Pakistan.
Nargis Shaheen, Naveeda Akhter Qureshi, Muhammad Zahid Qureshi, Huma Fatima, Muhammad Afzal, Saleh S Alhewairini
Acta Tropica.2020; 206: 105434. CrossRef
Clinical, microscopic and molecular presentation in pediatric versus adult old world cutaneous Leishmaniasis
Gabriel Dunya, Asif Loya, Suad Taraif, Mohammad Adib Houreih, Ibrahim Khalifeh
Pediatric Dermatology.2020; 37(4): 656. CrossRef
Clinical and Epidemiological study of Cutaneous Leishmaniasis from two tertiary care hospitals of Jammu and Kashmir: an Emerging Disease in North India
Shagufta Rather, Mashkoor Wani, Faizan Younus Shah, Safia Bashir, Atiya Yaseen, Firdous Ahmad Giri, Rajesh Sharma, Sumaya Zeerak, Yasmeen Jabeen, Iffat Hassan, Devraj Dogra, Ruby Rishi
International Journal of Infectious Diseases.2020;[Epub] CrossRef
Novel Loci Controlling Parasite Load in Organs of Mice Infected With Leishmania major, Their Interactions and Sex Influence
Tatyana Kobets, Marie Čepičková, Valeriya Volkova, Yahya Sohrabi, Helena Havelková, Milena Svobodová, Peter Demant, Marie Lipoldová
Frontiers in Immunology.2019;[Epub] CrossRef
Mucosal leishmaniasis: A forgotten disease, description and identification of species in 50 Colombian cases
Sandra Muvdi-Arenas, Clemencia Ovalle-Bracho
Biomédica.2019; 39(Supl. 2): 58. CrossRef
Spatial modeling of cutaneous leishmaniasis in Iranian army units during 2014-2017 using a hierarchical Bayesian method and the spatial scan statistic
Erfan Ayubi, Mohammad Barati, Arasb Dabbagh Moghaddam, Ali Reza Khoshdel
Epidemiology and Health.2018; 40: e2018032. CrossRef
Spatial modeling of cutaneous leishmaniasis in Iran from 1983 to 2013
Kourosh Holakouie-Naieni, Ehsan Mostafavi, Ali Darvishi Boloorani, Mehdi Mohebali, Reza Pakzad
Acta Tropica.2017; 166: 67. CrossRef
Reprint of “Spatial modeling of Cutaneous Leishmaniasis in Iran from 1983 to 2013”
Kourosh Holakouie-Naieni, Ehsan Mostafavi, Ali Darvishi Boloorani, Mehdi Mohebali, Reza Pakzad
Acta Tropica.2017; 165: 90. CrossRef
Sporotrichoid leishmaniasis: a cross-sectional clinical, epidemiological and laboratory study in Rio de Janeiro State, Brazil
Livia Martins Veloso de Carvalho, Maria Inês Fernandes Pimentel, Fátima Conceição-Silva, Érica de Camargo Ferreira e Vasconcellos, Cláudia Maria Valete-Rosalino, Marcelo Rosandiski Lyra, Mariza de Matos Salgueiro, Maurício Naoto Saheki, Maria de Fátima Ma
Revista do Instituto de Medicina Tropical de São Paulo.2017;[Epub] CrossRef
Spatio-temporal analysis of cutaneous leishmaniasis using geographic information system among Iranian Army Units and its comparison with the general population of Iran during 2005–2014
Reza Pakzad, Arasb Dabbagh-Moghaddam, Mehdi Mohebali, Saeid Safiri, Mohammad Barati
Journal of Parasitic Diseases.2017; 41(4): 1114. CrossRef
Epidemiological trend of cutaneous leishmaniasis in two endemic focus of disease, south of Iran
Moosa Khosravani, Zahra Nasiri, Davood Keshavarz, Azam Rafat-Panah
Journal of Parasitic Diseases.2016; 40(4): 1609. CrossRef
Head and neck cutaneous leishmania: clinical characteristics, microscopic features and molecular analysis in a cohort of 168 cases
Gabriel Dunya, Robert Habib, Roger V. Moukarbel, Ibrahim Khalifeh
European Archives of Oto-Rhino-Laryngology.2016; 273(11): 3819. CrossRef
Pediatric Cutaneous Leishmaniasis in an Endemic Region in Turkey: A Retrospective Analysis of 8786 Cases during 1998-2014
Mustafa Aksoy, Nebiye Doni, Hatice Uce Ozkul, Yavuz Yesilova, Nurittin Ardic, Abdullah Yesilova, Jennifer Ahn-Jarvis, Steve Oghumu, Cesar Terrazas, Abhay R. Satoskar, Paulo Filemon Pimenta
PLOS Neglected Tropical Diseases.2016; 10(7): e0004835. CrossRef
Geographic Information System‐Based Analysis of the Spatial and Spatio‐Temporal Distribution of Zoonotic Cutaneous Leishmaniasis in Golestan Province, North‐East of Iran
A. Mollalo, A. Alimohammadi, M. R. Shirzadi, M. R. Malek
Zoonoses and Public Health.2015; 62(1): 18. CrossRef
Systemic Meglumine Antimoniate in Cutaneous Leishmaniasis of Children: Clinical and Laboratory Complications
P. Layegh, Z. Khademi, M. Afzal Aghaee, T. Moghiman
Journal of the Pediatric Infectious Diseases Society.2015; 4(4): 356. CrossRef
Prevalence and epidemiologic profile of acute cutaneous leishmaniasis in an endemic focus, Southwestern Iran
Mohammad-Hossein Feiz-Haddad, Hamid Kassiri, Niusha Kasiri, Assiae Panahandeh, Masoud Lotfi
Journal of Acute Disease.2015; 4(4): 292. CrossRef
Epidemiological Aspects of Cutaneous Leishmaniasis in Iran
Salman Khazaei, Abdollah Mohammadian Hafshejani, Mohammad Saatchi, Hamid Salehiniya, Shahrzad Nematollahi
Archives of Clinical Infectious Diseases.2015;[Epub] CrossRef
Knowledge, attitude, and practices related to cutaneous leishmaniasis in an endemic focus of cutaneous leishmaniasis, Southern Iran
Bahador Sarkari, Asgari Qasem, Mohammad Reza Shafaf
Asian Pacific Journal of Tropical Biomedicine.2014; 4(7): 566. CrossRef
Pediatric Cutaneous Leishmaniasis in an Endemic Region in India
Abhay R. Satoskar, Saurabh Agrawal, Kanika Khandelwal, Steve Oghumu, Ram A. Bumb, Poonam Salotra
The American Journal of Tropical Medicine and Hygiene.2014; 91(5): 901. CrossRef
Overexpression of Ubiquitin and Amino Acid Permease Genes in Association with Antimony Resistance in Leishmania tropica Field Isolates
Elham Kazemi-Rad, Mehdi Mohebali, Mohammad Bagher Khadem-Erfan, Homa Hajjaran, Ramtin Hadighi, Ali Khamesipour, Sassan Rezaie, Mojtaba Saffari, Reza Raoofian, Mansour Heidari
The Korean Journal of Parasitology.2013; 51(4): 413. CrossRef
A newly emerged cutaneous leishmaniasis focus in central Iran
Mahmoud Nateghi Rostami, Abedin Saghafipour, Ehsan Vesali
International Journal of Infectious Diseases.2013; 17(12): e1198. CrossRef
The Incidence Rate of Cutaneous Leishmaniasis in Behbahan County of Khuzestan Province, Southwest of Iran
Khadijeh Shemshad, Hamid Kassiri, Samaneh Shojaee
Jundishapur Journal of Microbiology.2013;[Epub] CrossRef
A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran
Mohammad Reza Shiee, Homa hajjaran, Mehdi Mohebali, Abbas Doroodgar, Masoomeh Hashemi saadat, Aref Teimouri, Hassan Afzali, Mohammad Reza Shirzadi
Asian Pacific Journal of Tropical Disease.2012; 2(6): 421. CrossRef
The prevalence of cutaneous leishmaniasis in school children in southwestern Iran, 2009
Hojat Asgari Nezhad, Mohammad Mirzaie, Iraj Sharifi, Mehdi Zarean, Mojtaba Norouzi
Comparative Clinical Pathology.2012; 21(5): 1065. CrossRef
Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007)
Hamid Kassiri, Narges Sharifinia, Mostafa Jalilian, Khadijeh Shemshad
Asian Pacific Journal of Tropical Disease.2012; 2: S382. CrossRef
Clinical Laboratory and Epidemiological Research on Cutaneous leishmaniasis in the South West of Iran
Hamid Kassiri, Khadijeh Shemshad, Ali Kassiri, Samaneh Shojaee, Narges Sharifinia, Masoomeh Shemshad
Archives of Clinical Infectious Diseases.2012; 7(4): 128. CrossRef
An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran
Mahin Farahmand, Hossein Nahrevanian, Hasti Atashi Shirazi, Sabah Naeimi, Zahra Farzanehnejad
The Brazilian Journal of Infectious Diseases.2011; 15(1): 17. CrossRef
Systemic Meglumine Antimoniate in Acute Cutaneous Leishmaniasis: Children versus Adults
Amir Ali Rahsepar, Pouran Layegh, Sara Rahsepar
The American Journal of Tropical Medicine and Hygiene.2011; 84(4): 539. CrossRef
Childhood and adult cutaneous leishmaniasis in Tunisia
Ines Zaraa, Faten Ishak, Rym Kort, Dalenda El Euch, Mourad Mokni, Emna Chaker, Amel Ben Osman
International Journal of Dermatology.2010; 49(7): 790. CrossRef
The changing profile of cutaneous leishmaniasis in a focus of the disease in Jahrom district, southern Iran
M. H. Davami, M. H. Motazedian, B. Sarkari
Annals of Tropical Medicine & Parasitology.2010; 104(5): 377. CrossRef
Nuclear Weapons and Neglected Diseases: The “Ten-Thousand-to-One Gap”
Peter J. Hotez
PLoS Neglected Tropical Diseases.2010; 4(4): e680. CrossRef
Evaluation of Modified Novy-MacNeal-Nicolle Medium for Isolation of Leishmania Parasites from Cutaneous Lesions of Patients in Iran
J. Saki, L. Akhlaghi, S. Maraghi, A.R. Meamar, M. Mohebali, H. Oormazdi, E. Razmjou, S. Khademvata, F. Rahim
Research Journal of Parasitology.2009; 4(2): 56. CrossRef
Leishmaniasis in Turkey: molecular characterization of Leishmania from human and canine clinical samples
Seray Ozensoy Toz, Abedelmajeed Nasereddin, Yusuf Ozbel, Hatice Ertabaklar, Gulnaz Culha, Naser Sevil, M. Ziya Alkan, Charles L. Jaffe
Tropical Medicine & International Health.2009; 14(11): 1401. CrossRef
Identification of Leishmania parasites in clinical samples obtained from cutaneous leishmaniasis patients using PCR-RFLP technique in endemic region, Sanliurfa province, in Turkey
Feridun Akkafa, Fuat Dilmec, Zuhre Alpua
Parasitology Research.2008; 103(3): 583. CrossRef
Atypical presentation of Old‐World cutaneous leishmaniasis, diagnosis and species identification by PCR
M Karamian, MH Motazedian, M Fakhar, K Pakshir, F Jowkar, H Rezanezhad
Journal of the European Academy of Dermatology and Venereology.2008; 22(8): 958. CrossRef

9,541 View
116 Download
Crossref

Effects of combined therapy with thalidomide and glucantime on leishmaniasis induced by Leishmania major in BALB/c mice: Ghassem Solgi, Amina Kariminia, Khossro Abdi, Majid Darabi, Behnaz Ghareghozloo; Korean J Parasitol 2006;44(1):55-61.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.55

Abstract
PDF

For treating Leishmania major infection in BALB/c mice, we used thalidomide in conjunction with glucantime. Groups of mice were challenged with 5 × 10³ metacyclic promastigotes of L. major subcutaneously. A week after the challenge, drug treatment was started and continued for 12 days. Thalidomide was orally administrated 30 mg/kg/day and glucantime was administrated intraperitoneally (200 mg/kg/day). It was shown that the combined therapy is more effective than single therapies with each one of the drugs since the foot pad swelling in the group of mice received thalidomide and glucantime was significantly decreased (0.9 ± 0.2 mm) compared to mice treated with either glucantime, thalidomide, or carrier alone (1.2 ± 0.25, 1.4 ± 0.3, and 1.7 ± 0.27 mm, respectively). Cytokine study showed that the effect of thalidomide was not dependent on IL-12; however, it up-regulated IFN-γ and down-regulated IL-10 production. Conclusively, thalidomide seems promising as a conjunctive therapy with antimony in murine model of visceral leishmaniasis.

Citations

Citations to this article as recorded by

Deciphering Molecular Mechanisms of Cutaneous Leishmaniasis, Pathogenesis and Drug Repurposing through Systems Biology
Fatemeh Saberi, Zeinab Dehghan, Zahra Taheri, Tayyebeh Pilehchi, Hakimeh Zali
Iranian Biomedical Journal.2024; 28(4): 179. CrossRef
A standardized intraperitoneal Glucantime™ for experimental treatment of cutaneous leishmaniasis caused by Leishmania amazonensis in BALB/c mice
Aline Ávila Brustolin, Nathália Martins Franzói, Áquila Carolina Fernandes Herculano Ramos-Milaré, Cínthia Akemi Tanoshi, Camila Alves Mota, Izabel Galhardo Demarchi, Maria Valdrinez Campana Lonardoni, Thaís Gomes Verzignassi Silveira
Experimental Parasitology.2022; 236-237: 108259. CrossRef
Effect of Synergism of Thalidomide and Liposomal Amphotericin-B on Leishmania tropica and Leishmania donovani Promastigote
Nasma I. Al-Mamary, Haitham L. Al-Hayali
Bionatura.2022; 7(2): 1. CrossRef
Thalidomide suppresses inflammation in adenine-induced CKD with uraemia in mice
Alexandre C. Santana, Sabrina Degaspari, Sergio Catanozi, Humberto Dellê, Larissa de Sá Lima, Cleonice Silva, Paula Blanco, Kim Solez, Cristoforo Scavone, Irene L. Noronha
Nephrology Dialysis Transplantation.2013; 28(5): 1140. CrossRef
Immunomodulation by chemotherapeutic agents against Leishmaniasis
Piu Saha, Debanjan Mukhopadhyay, Mitali Chatterjee
International Immunopharmacology.2011; 11(11): 1668. CrossRef
Immunomodulatory effect of picroliv on the efficacy of paromomycin and miltefosine in combination in experimental visceral leishmaniasis
Shraddha A. Sane, Nishi Shakya, Suman Gupta
Experimental Parasitology.2011; 127(2): 376. CrossRef
Potential utility of hyperbaric oxygen therapy and propolis in enhancing the leishmanicidal activity of glucantime
Diana Copi Ayres, Thiago Antonio Fedele, Maria Cristina Marcucci, Selma Giorgio
Revista do Instituto de Medicina Tropical de São Paulo.2011; 53(6): 329. CrossRef
Anni 2.0: a multipurpose text-mining tool for the life sciences
Rob Jelier, Martijn J Schuemie, Antoine Veldhoven, Lambert CJ Dorssers, Guido Jenster, Jan A Kors
Genome Biology.2008;[Epub] CrossRef

8,833 View
94 Download
Crossref

Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice: Mohammad Reza Mohammadi, Majid Zeinali, Sussan K. Ardestani, Amina Kariminia; Korean J Parasitol 2006;44(1):43-48.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.43

Abstract
PDF

Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are increasing non-comparative studies on immune responses against isolated antigens of L. major in different murine strains. The aim of the present study was to find out whether there is an antigen that can induce protective immune response in resistant and susceptible murine strains. To do that, crude antigenic extract of procyclic and metacyclic promastigotes of L. major was prepared and subjected to SDS-PAGE electrophoresis. Western-blotting was used to search for antigen(s) capable of raising high antibody level of IgG2a versus IgG1 in the sera of both infected resistant and susceptible strains. Two novel antigens from metacyclic promastigotes of L. major (140 and 152 kDa) were potentially able to induce specific dominant IgG2a responses in BALB/c and C57BL/6 mice. The 2 antigens also reacted with IgG antibody of cutaneous leishmaniasis patients. We confirm that 140 and 152 kDa proteins of L. major promastigotes are inducing IgG production in mice and humans.

Citations

Citations to this article as recorded by

Evaluation of Leishmania homolog of activated C kinase (LACK) of Leishmania donovani in comparison to glycoprotein 63 as a vaccine candidate against visceral leishmaniasis
Nicky Didwania, Sudipta Bhowmick, Abdus Sabur, Anirban Bhattacharya, Nahid Ali, Monica R. Mugnier
Microbiology Spectrum.2025;[Epub] CrossRef
Diphencyprone reduces the CD8+ lymphocytes and IL-4 and enhences IgG2a/IgG1 ratio in pathogenicity of acute leishmania major infection in BALB/c mice
Pourandokht Mousavian, Vahid Mashayekhi Goyonlo, Mohammad Javanbakht, Mahmoud Reza Jafari, Hamidreza Moosavian, Monovar Afzal Aghaei, Mohammadreza Malekzadeh
Cytokine.2024; 184: 156792. CrossRef
Immunological characterization of rLdTCP1γ for its prophylactic potential against visceral leishmaniasis in hamster model
Apeksha Anand, Deep Chandra Balodi, Karthik Ramalingam, Shailendra Yadav, Neena Goyal
Molecular Immunology.2022; 141: 33. CrossRef
Adjuvanted vaccines driven protection against visceral infection in BALB/c mice by Leishmania donovani
Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur
Microbial Pathogenesis.2021; 151: 104733. CrossRef
Immune induction by adjuvanted Leishmania donovani vaccines against the visceral leishmaniasis in BALB/c mice
Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur
Immunobiology.2021; 226(2): 152057. CrossRef
Adjuvant effects of TLR agonist gardiquimod admixed with Leishmania vaccine in mice model of visceral leishmaniasis
Deepak Kumar Goyal, Poonam Keshav, Sukhbir Kaur
Infection, Genetics and Evolution.2021; 93: 104947. CrossRef
The potential of live attenuated vaccines against Cutaneous Leishmaniasis
A. Zabala-Peñafiel, D. Todd, H. Daneshvar, R. Burchmore
Experimental Parasitology.2020; 210: 107849. CrossRef
Immunization with a recombinant fusion protein protects mice against Helicobacter pylori infection
Amir Ghasemi, Nazanin Mohammad, Josef Mautner, Mehrnaz Taghipour Karsabet, Jafar Amani, Abolfazl Ardjmand, Zarichehr Vakili
Vaccine.2018; 36(34): 5124. CrossRef
Evaluation of vaccinal effectiveness of preparations containing membrane antigens of Leishmania (L.) amazonensis in experimental cutaneous leishmaniasis model
João G. Ribeiro, Amália S. Ferreira, Sharon R.A. Macedo, Norton R.D.L.P. Rossi, Mayara C.P. da Silva, Rosane N.M. Guerra, Neuza B. de Barros, Roberto Nicolete
International Immunopharmacology.2017; 47: 227. CrossRef
Evaluation of the immunoprophylactic potential of a killed vaccine candidate in combination with different adjuvants against murine visceral leishmaniasis
Ankita Thakur, Harpreet Kaur, Sukhbir Kaur
Parasitology International.2015; 64(1): 70. CrossRef
Ox40L–Ox40 pathway plays distinct roles in regulating Th2 responses but does not determine outcome of cutaneous leishmaniasis caused by Leishmania mexicana and Leishmania major
Rashmi Tuladhar, Steve Oghumu, Ran Dong, Allison Peterson, Arlene H. Sharpe, Abhay R. Satoskar
Experimental Parasitology.2015; 148: 49. CrossRef
Identification of immunodominant Leishmania major antigenic markers of the early C57BL/6 and BALB/c mice infection stages
A. Sassi, O. Kaak, A. Ben Ammar Elgaied
Parasite Immunology.2015; 37(10): 544. CrossRef
Th1 Platform Immune Responses Against Leishmania major Induced by Thiol-Specific Antioxidant-Based DNA Vaccines
Fatemeh Tabatabaie, Mehdi Mahdavi, Sobhan Faezi, Abdolhossein Dalimi, Zohreh Sharifi, Lame Akhlaghi, Fatemeh Ghaffarifar
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef
Studies on the protective efficacy and immunogenicity of Hsp70 and Hsp83 based vaccine formulations in Leishmania donovani infected BALB/c mice
Jaspreet Kaur, Tejinder Kaur, Sukhbir Kaur
Acta Tropica.2011; 119(1): 50. CrossRef
Protective effect of lectin from Synadenium carinatum on Leishmania amazonensis infection in BALB/c mice
Sandra R. Afonso-Cardoso, Flávio H. Rodrigues, Márcio A.B. Gomes, Adriano G. Silva, Ademir Rocha, Aparecida H.B. GuimarÃes, Ignês Candeloro, Sílvio Favoreto, Marcelo S. Ferreira, Maria A. de Souza
The Korean Journal of Parasitology.2007; 45(4): 255. CrossRef
Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes
Majid Zeinali, Sussan K. Ardestani, Amina Kariminia
The Korean Journal of Parasitology.2007; 45(4): 287. CrossRef

7,739 View
73 Download
Crossref

Production of nitric oxide by murine macrophages induced by lipophosphoglycan of Leishmania major: Gholamreza Kavoosi, Sussan K. Ardestani, Amina Kariminia, Zahra Tavakoli; Korean J Parasitol 2006;44(1):35-41.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.35

Abstract
PDF

Protozoan parasites of the genus Leishmania cause a number of important human diseases. One of the key determinants of parasite infectivity and survival is the surface glycoconjugate lipophosphoglycan (LPG). In addition, LPG is shown to be useful as a transmission blocking vaccine. Since culture supernatant of parasite promastigotes is a good source of LPG, we made attempts to characterize functions of the culture supernatant, and membrane LPG isolated from metacyclic promastigotes of Leishmania major. The purification scheme included anion-exchange chromatography, hydrophobic interaction chromatography and cold methanol precipitation. The purity of supernatant LPG (sLPG) and membrane LPG (mLPG) was determined by SDS-PAGE and thin layer chromatography. The effect of mLPG and sLPG on nitric oxide (NO) production by murine macrophages cell line (J774.1A) was studied. Both sLPG and mLPG induced NO production in a dose dependent manner but sLPG induced significantly higher amount of NO than mLPG. Our results show that sLPG is able to promote NO production by murine macrophages.

Citations

Citations to this article as recorded by

Review on the Role of Host Immune Response in Protection and Immunopathogenesis during Cutaneous Leishmaniasis Infection
Teshager Dubie, Yasin Mohammed, Patrice Xavier Petit
Journal of Immunology Research.2020;[Epub] CrossRef
Metformin partially reverses the inhibitory effect of co-culture with ER-/PR-/HER2+ breast cancer cells on biomarkers of monocyte antitumor activity
Zoheir Dahmani, Lynda Addou-Klouche, Florence Gizard, Sara Dahou, Aida Messaoud, Nihel Chahinez Djebri, Mahmoud Idris Benaissti, Meriem Mostefaoui, Hadjer Terbeche, Wafa Nouari, Marwa Miliani, Gérard Lefranc, Anne Fernandez, Ned J. Lamb, Mourad Aribi, Sal
PLOS ONE.2020; 15(10): e0240982. CrossRef
Future Prospects in the Treatment of Parasitic Diseases: 2-Amino-1,3,4-Thiadiazoles in Leishmaniasis
Georgeta Serban
Molecules.2019; 24(8): 1557. CrossRef
A new approach for development of vaccine against visceral leishmaniasis: Lipophosphoglycan and polyacrylic acid conjugates
Adil M. Allahverdiyev, Rabia Cakir Koc, Melahat Bagirova, Serhat Elcicek, Serap Yesilkir Baydar, Olga Nehir Oztel, Emrah Sefik Abamor, Sezen Canim Ates, Murat Topuzogullari, Sevil Isoglu Dincer, Zeynep Akdeste
Asian Pacific Journal of Tropical Medicine.2017; 10(9): 877. CrossRef
Reactive Oxygen Species and Nitric Oxide in Cutaneous Leishmaniasis
Maria Fátima Horta, Bárbara Pinheiro Mendes, Eric Henrique Roma, Fátima Soares Motta Noronha, Juan Pereira Macêdo, Luciana Souza Oliveira, Myrian Morato Duarte, Leda Quercia Vieira
Journal of Parasitology Research.2012; 2012: 1. CrossRef
DNA damage and nitric oxide production in mice following infection with L. chagasi
Larissa Ragozo Cardoso de Oliveira, Gláucia Aparecida Gomes Cezário, Carlos Roberto Gonçalves de Lima, Vanessa Cristina Nicolete, Eliana Peresi, Maria Tereza de Síbio, Mariele Cristina Modolo Picka, Sueli Aparecida Calvi
Mutation Research/Genetic Toxicology and Environmental Mutagenesis.2011; 723(2): 177. CrossRef
Antileishmanial Activity of 1,3,4-Thiadiazolium-2-Aminide in Mice Infected withLeishmania amazonensis
Raquel F. Rodrigues, Karen S. Charret, Edson F. da Silva, Áurea Echevarria, Verônica F. Amaral, Leonor L. Leon, Marilene M. Canto-Cavalheiro
Antimicrobial Agents and Chemotherapy.2009; 53(2): 839. CrossRef
Pharmacological evaluation of anti-leishmanial activity by in vivo nitric oxide modulation in Balb/c mice infected with Leishmania major MRHO/IR/75/ER: An Iranian strain of cutaneous leishmaniasis
Hossein Nahrevanian, Mahin Farahmand, Zohreh Aghighi, Mehdi Assmar, Aref Amirkhani
Experimental Parasitology.2007; 116(3): 233. CrossRef