Anopheles fluviatilis James (Diptera: Culicidae) is one of the known malaria vectors in south and southeastern Iran. Earlier ITS2 sequences analysis of specimens from Iran demonstrated only a single genotype that was identical to species Y in India, which is also the same as species T. We identified 2 haplotypes in the An. fluviatilis populations of Iran based on differences in nucleotide sequences of D3 domain of the 28S locus of ribosomal DNA (rDNA). Comparison of sequence data from 44 Iranian specimens with those publicly available in the Genbank database showed that all of the 28S-D3 sequences from Kazeroun and Khesht regions in Fars Province were identical to the database entry representing species U in India. In other regions, all the individuals showed heterozygosity at the single nucleotide position, which identifies species U and T. It is argued that the 2 species may co-occur in some regions and hybridize; however, the heterozygosity in the 28S-D3 locus was not reflected in ITS2 sequences and this locus for all individuals was identical to species T. This study shows that in a newly diverged species, like members of An. fluviatilis complex, a single molecular marker may not be sufficiently discriminatory to identify all the taxa over a vast geographical area. In addition, other molecular markers may provide more reliable information for species discrimination.
Citations
Citations to this article as recorded by
Are members of the Anopheles fluviatilis complex conspecific? Om P. Singh, Ankita Sindhania, Gunjan Sharma, Shobhna Mishra, Surya K. Sharma, Piyoosh K. Singh, Manoj K. Das Acta Tropica.2021; 224: 106149. CrossRef
A detailed review of the mosquitoes (Diptera: Culicidae) of Iran and their medical and veterinary importance Shahyad Azari-Hamidian, Behzad Norouzi, Ralph E. Harbach Acta Tropica.2019; 194: 106. CrossRef
Spatial changes in the distribution of malaria vectors during the past 5 decades in Iran A. Salahi-Moghaddam, A. Khoshdel, H. Dalaei, K. Pakdad, G.G. Nutifafa, M.M. Sedaghat Acta Tropica.2017; 166: 45. CrossRef
An evaluation of the
suitability
of COI and COII gene variation for reconstructing the phylogeny of, and identifying cryptic species in, anopheline mosquitoes (Diptera Culicidae)
Gang Wang, Chunxiao Li, Wei Zheng, Fenglin Song, Xiaoxia Guo, Zhonghua Wu, Peng Luo, Yongyao Yang, Lei He, Tongyan Zhao Mitochondrial DNA Part A.2017; 28(5): 769. CrossRef
An integrative approach to species discrimination in the Anagrus atomus group sensu stricto (Hymenoptera: Mymaridae), with a description of a new species Francesco Nugnes, Umberto Bernardo, Gennaro Viggiani Systematics and Biodiversity.2017; 15(6): 582. CrossRef
Molecular Phylogenetic Analysis of the SubgeneraAnophelesandCellia(Diptera: Culicidae) Based on Nuclear Ribosomal Sequences G. Wang, C. Li, X. Guo, D. Xing, Y. Dong, T. Zhao African Entomology.2014; 22(3): 660. CrossRef
Molecular phylogenetic analysis of Anopheles and Cellia subgenus anophelines (Diptera: Culicidae) in temperate and tropical regions of Iran Saber Gholizadeh, Navid Dinparast Djadid, Behzad Nouroozi, Mojtaba Bekmohammadi Acta Tropica.2013; 126(1): 63. CrossRef
Mosquito fauna (Diptera: Culicidae) of the Iranian islands in the Persian Gulf II. Greater Tonb, Lesser Tonb and Kish Islands M. Khoobdel, S. Azari-Hamidian, A.A. Hanafi-Bojd Journal of Natural History.2012; 46(31-32): 1939. CrossRef
Malaria elimination trend from a hypo-endemic unstable active focus in southern Iran: predisposing climatic factors M D Moemenbellah-Fard, V Saleh, O Banafshi, T Dabaghmanesh Pathogens and Global Health.2012; 106(6): 358. CrossRef
Spatio—temporal distribution of malaria vectors (Diptera: Culicidae) across different climatic zones of Iran Ahmad Ali Hanafi-Bojd, Shahyad Azari-Hamidian, Vatandoost Hassan, Charrahy Zabihollah Asian Pacific Journal of Tropical Medicine.2011; 4(6): 498. CrossRef
First
Prev
