Raillietina species are prevalent in domestic chickens (Gallus gallus domesticus) in Phayao province, northern Thailand. Their infection may cause disease and death, which affects the public health and economic situation in chicken farms. The identification of Raillietina has been based on morphology and molecular analysis. In this study, morphological observations using light (LM) and scanning electron microscopies (SEM) coupled with molecular analysis of the internal transcribed spacer 2 (ITS2) region and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) gene were employed for precise identification and phylogenetic relationship studies of Raillietina spp. Four Raillietina species, including R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp., were recovered in domestic chickens from 4 districts in Phayao province, Thailand. LM and SEM observations revealed differences in the morphology of the scolex, position of the genital pore, number of eggs per egg capsule, and rostellar opening surface structures in all 4 species. Phylogenetic relationships were found among the phylogenetic trees obtained by the maximum likelihood and distance-based neighbor-joining methods. ITS2 and ND1 sequence data recorded from Raillietina sp. appeared to be monophyletic. The query sequences of R. echinobothrida, R. tetragona, R. cesticillus, and Raillietina sp. were separated according to the different morphological characters. This study confirmed that morphological studies combined with molecular analyses can differentiate related species within the genus Raillietina in Thailand.
Citations
Citations to this article as recorded by
Development of a duplex loop-mediated isothermal amplification together with lateral flow dipstick assay for the detection and discrimination of parasitic infections in chickens between cestodes belonging to genus Raillietina and trematodes in family Echi Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth Research in Veterinary Science.2025; 185: 105539. CrossRef
What's worming Egyptian chickens: Morphological and molecular identification of Raillietina echinobothrida and Ascaridia galli in domestic chickens in Egypt Ayman N. Elsayed, Wafaa Fayez Abd El-Hamed, Abd Elsattar M. Metwally, Nahed S. Amer, Abdelbaset E. Abdelbaset, Mohamed Abdo Rizk, Adel Abdelkhalek, Refaat Ras Veterinary Parasitology: Regional Studies and Reports.2025; 66: 101372. CrossRef
Raillietina cesticillus infection causes reduced egg production in chickens in a windowless poultry house Fumi OSHIMA, Akiko MIYAJI, Masaki KONNAI, Saki ITO, Hinako SUZUKI, Naoyuki AIHARA, Takanori SHIGA, Kensuke TAIRA Journal of Veterinary Medical Science.2024; 86(2): 224. CrossRef
Assay for the simultaneous detection of Raillietina spp. (R. echinobothrida, R. tetragona, and R. cesticillus) and Ascaridia galli infection in chickens using duplex loop-mediated isothermal amplification integrated with a lateral flow dipstick assay Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth Veterinary Parasitology.2024; 328: 110174. CrossRef
Phylogenetic relationships and systematics of tapeworms of the family Davaineidae (Cestoda, Cyclophyllidea), with emphasis on species in rodents Voitto Haukisalmi, Alexis Ribas, Jean-Pierre Hugot, Serge Morand, Kittipong Chaisiri, Kerstin Junker, Sonja Matthee, Andrea Spickett, Jukka T. Lehtonen, Carlos Feliu, Heikki Henttonen Folia Parasitologica.2024;[Epub] CrossRef
An ethnopharmacological approach to evaluate antiparasitic and health-promoting abilities of Pueraria tuberosa (Willd.) DC. in livestock Suman Kalyan Mandal, Niladri Mukherjee, Anindya Sundar Ray, Samik Hazra, Sathi Saha, Swetarka Das, Nikhilesh Joardar, Saradindu Saha, Santi Prasad Sinha Babu, Chowdhury Habibur Rahaman, Marcello Iriti PLOS ONE.2024; 19(7): e0305667. CrossRef
A simple color absorption analysis of colorimetric loop-mediated isothermal amplification for detection of Raillietina spp. in clinical samples using a 3D-printed tube holder coupled with a smartphone camera and notebook screen Wasin Panich, Chokchai Puttharugsa, Thanawan Tejangkura, Thapana Chontananarth Microchimica Acta.2024;[Epub] CrossRef
Morphological and phylogenetic analysis of Raillietina spp. in indigenous chickens (Gallus gallus domesticus) in Bangladesh Tanbin Rubaiya Siddiqui, Md. Razibul Hoque, Babul Chandra Roy, Anisuzzaman, Mohammad Zahangir Alam, Mst. Sawda Khatun, Anita Rani Dey Saudi Journal of Biological Sciences.2023; 30(10): 103784. CrossRef
Feasibility of a DNA biosensor assay based on loop-mediated isothermal amplification combined with a lateral flow dipstick assay for the visual detection of Ascaridia galli eggs in faecal samples Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth Avian Pathology.2023; 52(3): 209. CrossRef
Anthelmintic efficacy of Holarrhena pubescens against Raillietina spp. of domestic fowl through ultrastructural, histochemical, biochemical and GLCM analysis Rachita Saha, Manjil Gupta, Rima Majumdar, Subrata Saha, Pradip Kumar Kar, Josué de Moraes PLOS ONE.2023; 18(9): e0282033. CrossRef
High-performance triplex PCR detection of three tapeworm species belonging to the genus Raillietina in infected poultry Wasin Panich, Sirapat Nak-on, Thapana Chontananarth Acta Tropica.2022; 232: 106516. CrossRef
Heteroplasmic mitochondrial genomes of a Raillietina tapeworm in wild Pangolin Merga Daba Tuli, Hongyi Li, Xi Pan, Song Li, Junqiong Zhai, Yajiang Wu, Wu Chen, Wanyi Huang, Yaoyu Feng, Lihua Xiao, Dongjuan Yuan Parasites & Vectors.2022;[Epub] CrossRef
Areca catechu L. and Anredera cordifolia (Ten) Steenis supplementation reduces faecal parasites and improves caecal histopathology in laying hens Retno Murwani, Endang Kusumanti, Elena N. Naumova International Journal of Veterinary Science and Medicine.2022; 10(1): 52. CrossRef
Intestinal cestode infection of Raillietina species in a 9 weeks old broiler in Umuahia, Abia State, Nigeria – A Case Report
Journal of Sustainable Veterinary and Allied Sciences.2022; : 123. CrossRef
Cestode Infection in the Digestive Tract of Ducks from Chicken Slaughterhouses in Bojong Gede and Cibinong Market, Bogor, West Java Lala Aviola, Risa Tiuria, Arifin Budiman Nugraha Jurnal Medik Veteriner.2022; 5(2): 149. CrossRef
Detection ofRaillietina saudiaefrom the domestic pigeon in Saudi Arabia through18Sand28S rDNAgenes S. Al‐Quraishy, R. Abdel‐Gaber, M.A. Dkhil, A.S. Abdel‐Baki, M. Alotaibi, W. Alhafidh, N. Al‐Houshany Letters in Applied Microbiology.2021; 72(1): 90. CrossRef
Preliminary data on Ascaridia galli infections in Gallus gallus domesticus and the development of a specific primer based on the NADH dehydrogenase subunit 4 Tanapat Watcharakranjanaporn, Metawee Sabaijai, Chadaporn Dunghungzin, Thapana Chontananarth Journal of Parasitic Diseases.2021; 45(2): 293. CrossRef
Molecular phylogenetic identification and morphological characteristics of Raillietina echinobothrida (Cestoda: Cyclophyllidea: Davaineidae) in commercial chickens in North China Ding Zhang, Guodong Wu, Xiaoyu Yang, Wenxia Tian, Nairui Huo Parasitology Research.2021; 120(4): 1303. CrossRef
Among the shapeshifters: parasite-induced morphologies in ants (Hymenoptera, Formicidae) and their relevance within the EcoEvoDevo framework Alice Laciny EvoDevo.2021;[Epub] CrossRef
Novel high-performance detection of Raillietina echinobothrida, Raillietina tetragona, and Raillietina cesticillus using loop-mediated isothermal amplification coupled with a lateral flow dipstick (LAMP-LFD) Wasin Panich, Thanawan Tejangkura, Thapana Chontananarth Veterinary Parasitology.2021; 292: 109396. CrossRef
Molecular detection of three intestinal cestode species (Raillietina echinobothrida, R. tetragona, R. cesticillus) from poultry in Thailand Wasin Panich, Thapana Chontananarth Avian Pathology.2021; 50(4): 321. CrossRef
Effect of Lysimachia ramosa Wall. Ex Duby and Its n-Butanol Fraction on Glycogen Content and Some Energy Related Enzymes in the Cestode, Raillietina echinobothrida Paulomi Dey, Bishnupada Roy Proceedings of the Zoological Society.2020; 73(3): 255. CrossRef
Molecular characterization of Raillietina isolates from the gastrointestinal tract of free range chickens (Gallus Gallus domesticus) from the southern region of Zimbabwe using the 18S rDNA gene T. Makwanise, S. Dube, M.S. Sibula Veterinary Parasitology: Regional Studies and Reports.2020; 20: 100389. CrossRef
Morphological and molecular appraisal of cyclophyllidean cestoda parasite Raillietina saudiae sp. nov. infecting the domestic pigeon Columba livia domestica and its role as a bio-indicator for environmental quality Saleh Al Quraishy, Rewaida Abdel-Gaber, Reem Alajmi, Mohamed A. Dkhil, Munirah Al Jawher, Kareem Morsy Parasitology International.2019; 71: 59. CrossRef
Toxocara vitulorum has been rarely reported in yaks at high altitudes and remote areas of Sichuan Province of Tibetan Plateau of China. The current study was designed to investigate the prevalence, associated risk factors, and phylogenetic characteristics of T. vitulorum in yak calves on the Qinghai Tibetan plateau. Fecal samples were collected from 891 yak calves and were examined for the presence of T. vitulorum eggs by the McMaster technique. A multivariable logistic regression model was employed to explore variables potentially associated with exposure to T. vitulorum infection. T. vitulorum specimens were collected from the feces of yaks in Hongyuan of Sichuan Province, China. DNA was extracted from ascaris. After PCR amplification, the sequencing of ND1 gene was carried out and phylogenetic analyses was performed by MEGA 6.0 software. The results showed that 64 (20.1%; 95% CI 15.8-24.9%), 75 (17.2; 13.8-21.1), 29 (40.9; 29.3-53.2), and 5 (7.6; 2.5-16.8) yak calves were detected out to excrete T. vitulorum eggs in yak calve feces in Qinghai, Tibet, Sichuan, and Gansu, respectively. The present study revealed that high infection and mortality by T. vitulorum is wildly spread on the Qinghai Tibetan plateau, China by fecal examination. Geographical origin, ages, and fecal consistencies are the risk factors associated with T. vitulorum prevalence by logistic regression analysis. Molecular detection and phylogenetic analysis of ND1 gene of T. vitulorum indicated that T. vitulorum in the yak calves on the Qinghai Tibetan plateau are homologous to preveiously studies reported.
Citations
Citations to this article as recorded by
Molecular characterization and genetic variability of Toxocara vitulorum from naturally infected buffalo calves for the first time in Bangladesh Hiranmoy Biswas, Nurnabi Ahmed, Babul Chandra Roy, Mohammad Manjurul Hasan, MD Khalilur Rahman, Md. Hasanuzzaman Talukder Parasitology.2024; 151(8): 795. CrossRef
RESEARCH PROGRESS ON MAJOR DISEASES OF PIGS AND YAKS ON THE QINGHAI-TIBET PLATEAU: A COMPREHENSIVE REVIEW FROM 1990 TO 2023 P Tian, Y Wang, H Zhang, H Yang, M.U. Rehman, F Kebzai, Y Li The Journal of Animal and Plant Sciences.2024; 34(4): 832. CrossRef
Application of Silver Nanoparticles in Parasite Treatment Ping Zhang, Jiahao Gong, Yan Jiang, Yunfeng Long, Weiqiang Lei, Xiuge Gao, Dawei Guo Pharmaceutics.2023; 15(7): 1783. CrossRef
Molecular detection and phylogenetic analysis of Toxocara vitulorum in feces and milk samples from naturally infected water buffaloes Osman Furkan Urhan, Ufuk Erol, Kursat Altay Research in Veterinary Science.2023; 162: 104952. CrossRef
In vitro anti-Toxocara vitulorum effect of silver nanoparticles Manar Ahmed Bahaaeldine, Manal El Garhy, Sohair R. Fahmy, Ayman Saber Mohamed Journal of Parasitic Diseases.2022; 46(2): 409. CrossRef
Antiprotozoal and Anthelmintic Activity of Zinc Oxide Nanoparticles José Rodrigues do Carmo Neto, Rhanoica Oliveira Guerra, Juliana Reis Machado, Anielle Christine Almeida Silva, Marcos Vinicius da Silva Current Medicinal Chemistry.2022; 29(12): 2127. CrossRef
The Complete Mitogenome of Toxocara vitulorum: Novel In-Sights into the Phylogenetics in Toxocaridae Yue Xie, Lidan Wang, Yijun Chen, Zhao Wang, Pengchen Zhu, Zun Hu, Xinfeng Han, Zhisheng Wang, Xuan Zhou, Zhicai Zuo Animals.2022; 12(24): 3546. CrossRef
Epidemiological Studies on Nematode Parasites of Domestic Geese (Anser anser f. domesticus) and First Molecular Identification and Phylogenetic Analysis of Heterakis dispar (Schrank, 1790) in Egypt Ismail Elshahawy, Mahmoud El-Siefy, Samia Fawy, Eman Mohammed Acta Parasitologica.2021; 66(4): 1297. CrossRef
Prevalence and risk factors of Toxocara vitulorum infection in buffalo calves in coastal, northeastern and northwestern regions of Bangladesh Hiranmoy Biswas, Babul Chandra Roy, Pallab Kumar Dutta, Mohammad Manjurul Hasan, Shanaz Parvin, Dipok Kumar Choudhury, Nurjahan Begum, Md. Hasanuzzaman Talukder Veterinary Parasitology: Regional Studies and Reports.2021; 26: 100656. CrossRef
Immunodiagnosis of anti-Toxocara vitulorum IgG antibodies by using commercially available bovine ELISA Kit in bovine of Potohar region Pakistan Samina Asghar Abbasi, Mazhar Qayum, Ruqayya Mehmood Baig, Mehvish Naseer Ahmad, Akbar Shah, Mujeeb ur Rahman, Haroon Acta Ecologica Sinica.2020; 40(1): 97. CrossRef
Impacts of different altitudes and natural drying times on lipolysis, lipid oxidation and flavour profile of traditional Tibetan yak jerky Ge Han, Lang Zhang, Qixuan Li, Yan Wang, Qian Chen, Baohua Kong Meat Science.2020; 162: 108030. CrossRef
Molecular characterization of ascaridoid parasites from captive wild carnivores in China using ribosomal and mitochondrial sequences Yue Xie, Yingxin Li, Xiaobin Gu, Yunjian Liu, Xuan Zhou, Lu Wang, Ran He, Xuerong Peng, Guangyou Yang Parasites & Vectors.2020;[Epub] CrossRef
Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park The Korean Journal of Parasitology.2020; 58(4): 403. CrossRef
Toxocariasis y vacunación para Toxocara: una revisión sistemática Dumar A. Jaramillo-Hernández, Luis F. Salazar-Garcés, Mónica M. Baquero-Parra, Carina Da Silva-Pinheiro, Neuza M. Alcantara-Neves Orinoquia.2020; 24(2): 79. CrossRef
Molecular characterization and phylogenetic analysis of Toxocara species in dogs, cattle and buffalo in Egypt O.A. Mahdy, W. M. Mousa, S. Z. Abdel-Maogood, S. M. Nader, S. Abdel-Radi Helminthologia.2020; 57(2): 83. CrossRef
The impact of Bacillus subtilis 18 isolated from Tibetan yaks on growth performance and gut microbial community in mice Aoyun Li, Xiong Jiang, Yaping Wang, Lihong Zhang, Hui Zhang, Khalid Mehmood, Zhixing Li, Muhammad Waqas, Jiakui Li Microbial Pathogenesis.2019; 128: 153. CrossRef
The serodiagnostic potential of recombinant proteins TES–30 and TES–120 in an indirect ELISA in the diagnosis of toxocariasis in cattle, horses, and sheep Lucas Moreira dos Santos, Rafael Amaral Donassolo, Maria Elisabeth Berne, Fábio Pereira Leivas Leite, Luciana Farias da Costa Avila, Carlos James Scaini, Ângela Nunes Moreira, Fabricio Rochedo Conceição, Henk D. F. H. Schallig PLOS ONE.2019; 14(3): e0213830. CrossRef
Analysis of the internal transcribed spacer region of
Ascaris suum
and
Ascaris lumbricoides
derived from free range Tibetan pigs
Kun Li, Houqiang Luo, Hui Zhang, Khalid Mehmood, Muhammad Shahzad, Lihong Zhang, Jiakui Li Mitochondrial DNA Part A.2018; 29(4): 624. CrossRef
Characterization of fungus microbial diversity in healthy and diarrheal yaks in Gannan region of Tibet Autonomous Prefecture Kun Li, Khalid Mehmood, Hui Zhang, Xiong Jiang, Muhammad Shahzad, Xiaoqian Dong, Jiakui Li Acta Tropica.2018; 182: 14. CrossRef
Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau Yantao Wu, Li Li, Guoqiang Zhu, Wenhui Li, Nianzhang Zhang, Shuangnan Li, Gang Yao, Wenjun Tian, Baoquan Fu, Hong Yin, Xingquan Zhu, Hongbin Yan, Wanzhong Jia Parasites & Vectors.2018;[Epub] CrossRef
Socio-economic burden of parasitic infections in yaks from 1984 to 2017 on Qinghai Tibetan Plateau of China—A review Kun Li, Muhammad Shahzad, Hui Zhang, Xiong Jiang, Khalid Mehmood, Xiaodong Zhao, Jiakui Li Acta Tropica.2018; 183: 103. CrossRef
Epidemiological investigation and risk factors of Echinococcus granulosus in yaks (Bos grunniens), Tibetan pigs and Tibetans on Qinghai Tibetan plateau Kun Li, Lihong Zhang, Hui Zhang, Zhixin Lei, Houqiang Luo, Khalid Mehmood, Muhammad Shahzad, Yanfang Lan, Meng Wang, Jiakui Li Acta Tropica.2017; 173: 147. CrossRef
First
Prev
