Parasites, Hosts and Diseases

"Selma Giorgio"

Original Articles

Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein: Solange dos Santos Costa, Marjorie de Assis Golim, Bartira Rossi-Bergmann, Fabio Trindade Maranh?o Costa, Selma Giorgio; Korean J Parasitol 2011;49(4):357-364.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.357

Abstract
PDF

Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have used the flagellated extracellular promastigote, the stage of parasite detected in the midgut of the sandfly vector; fewer studies have been performed with amastigotes, the stage of parasite detected in mammals. In this study, comparisons were made regarding the efficiency for in vitro G418 selection of GFP-Leishmania amazonensis promastigotes and amastigotes and the use of in vivo G418 selection. The GFP-promastigotes retained episomal plasmid for a prolonged period and G418 treatment was necessary and efficient for in vitro selection. In contrast, GFP-amastigotes showed low retention of the episomal plasmid in the absence of G418 selection and low sensitivity to antibiotics in vitro. The use of protocols for G418 selection using infected BALB/c mice also indicated low sensitivity to antibiotics against amastigotes in cutaneous lesions.

Citations

Citations to this article as recorded by

Nanocrystallization Effectively Improves the Oral Efficacy of an Antileishmanial Chalcone
Maria Paula Gonçalves Borsodi, Wallace Pacienza-Lima, Jaqueline Correia Villaça Menezes, Douglas Escrivani-Oliveira, Natalia Arruda-Costa, Alcides José Monteiro da Silva, Lucio Mendes Cabral, Patrick G. Steel, Ariane de Jesus Sousa-Batista, Bartira Rossi-
Pharmaceutics.2025; 17(4): 399. CrossRef
Reporter gene systems: A powerful tool for Leishmania studies
Romário Lopes Boy, Ahyun Hong, Juliana Ide Aoki, Lucile Maria Floeter-Winter, Maria Fernanda Laranjeira-Silva
Current Research in Microbial Sciences.2022; 3: 100165. CrossRef
In Vivo Safety and Efficacy of Chalcone-Loaded Microparticles with Modified Polymeric Matrix against Cutaneous Leishmaniasis
Ariane de J. Sousa-Batista, Natalia Arruda-Costa, Wallace Pacienza-Lima, Felipe Carvalho-Gondim, Rosiane F. Santos, Silvia A. G. Da-Silva, Maria Inês Ré, Bartira Rossi-Bergmann
Pharmaceutics.2022; 15(1): 51. CrossRef
Antileishmanial Chemotherapy through Clemastine Fumarate Mediated Inhibition of the Leishmania Inositol Phosphorylceramide Synthase
John G. M. Mina, Rebecca L. Charlton, Edubiel Alpizar-Sosa, Douglas O. Escrivani, Christopher Brown, Amjed Alqaisi, Maria Paula G. Borsodi, Claudia P. Figueiredo, Emanuelle V. de Lima, Emily A. Dickie, Wenbin Wei, Robson Coutinho-Silva, Andy Merritt, Terr
ACS Infectious Diseases.2021; 7(1): 47. CrossRef
Ex Vivo Phenotypic Screening of Two Small Repurposing Drug Collections Identifies Nifuratel as a Potential New Treatment against Visceral and Cutaneous Leishmaniasis
Bárbara Domínguez-Asenjo, Camino Gutiérrez-Corbo, María Álvarez-Bardón, Yolanda Pérez-Pertejo, Rafael Balaña-Fouce, Rosa M. Reguera
ACS Infectious Diseases.2021; 7(8): 2390. CrossRef
Encapsulation in lipid-core nanocapsules improves topical treatment with the potent antileishmanial compound CH8
Douglas O Escrivani, Milene Valéria Lopes, Fernanda Poletto, Stela Regina Ferrarini, Ariane J. Sousa-Batista, Patrick G. Steel, Sílvia Stanisçuaski Guterres, Adriana Raffin Pohlmann, Bartira Rossi-Bergmann
Nanomedicine: Nanotechnology, Biology and Medicine.2020; 24: 102121. CrossRef
Single-dose treatment for cutaneous leishmaniasis with an easily synthesized chalcone entrapped in polymeric microparticles
Ariane J. Sousa-Batista, Natalia Arruda-Costa, Douglas O. Escrivani, Franceline Reynaud, Patrick G. Steel, Bartira Rossi-Bergmann
Parasitology.2020; 147(9): 1032. CrossRef
Broad Spectrum and Safety of Oral Treatment with a Promising Nitrosylated Chalcone in Murine Leishmaniasis
Ariane J. Sousa-Batista, Douglas Escrivani-Oliveira, Camila Alves Bandeira Falcão, Cintia Iana Monteiro da Silva Philipon, Bartira Rossi-Bergmann
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef
EGFP reporter protein: its immunogenicity in Leishmania-infected BALB/c mice
Samira Seif, Fereshteh Kazemi, Elham Gholami, Negar Seyed, Yasaman Taslimi, Sima Habibzadeh, Bahareh Azarian, Shahram Jamshidi, Mehrdad Hashemi, Sima Rafati, Tahereh Taheri
Applied Microbiology and Biotechnology.2016; 100(9): 3923. CrossRef
Development of Leishmania donovani stably expressing DsRed for flow cytometry-based drug screening using chalcone thiazolyl-hydrazone as a new antileishmanial target
Anil Kumar Jaiswal, K. Bhaskara Rao, Pragati Kushwaha, Keerti Rawat, Ram K. Modukuri, Prashant Khare, Sumit Joshi, Shikha Mishra, Ambak Rai, Koneni V. Sashidhara, Anuradha Dube
International Journal of Antimicrobial Agents.2016; 48(6): 695. CrossRef
Evaluation of the leishmanicidal and cytotoxic effects of inhibitors for microorganism metabolic pathway enzymes
Ademar de Mesquita Barbosa, Solange dos Santos Costa, Josmar Rodrigues da Rocha, Carlos Alberto Montanari, Selma Giorgio
Biomedicine & Pharmacotherapy.2015; 74: 95. CrossRef
An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein
Marcele N. Rocha, Célia M. Corrêa, Maria N. Melo, Stephen M. Beverley, Olindo Assis Martins-Filho, Ana Paula Madureira, Rodrigo P. Soares
Diagnostic Microbiology and Infectious Disease.2013; 75(3): 282. CrossRef

9,099 View
84 Download
Crossref

A Novel Organotellurium Compound (RT-01) as a New Antileishmanial Agent: Camila B?rbara Cantalupo Lima, Wagner Welber Arrais-Silva, Rodrigo Luiz Oliveira Rodrigues Cunha, Selma Giorgio; Korean J Parasitol 2009;47(3):213-218.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.213

Abstract
PDF

Leishmaniasis is a neglected disease and endemic in developing countries. A lack of adequate and definitive chemotherapeutic agents to fight against this infection has led to the investigation of numerous compounds. The aim of this study was to investigate the effect of RT-01, an organotellurane compound presenting biological activities, in 2 experimental systems against Leishmania amazonensis. The in vitro system consisted of promastigotes and amastigotes forms of the parasite, and the in vivo system consisted of L. amazonensis infected BALB/c mice, an extremely susceptible mouse strain. The compound proved to be toxic against promastigotes and amastigotes. The study also showed that treatment with RT-01 produces an effect similar to that treatment with the reference antimonial drug, Glucantime, in L. amazonensis infected mice. The best results were obtained following RT-01 intralesional administration (720 ?g/kg/day); mice showed significant delay in the development of cutaneous lesions and decreased numbers of parasites obtained from the lesions. Significant differences in tissue pathology consisted mainly of no expressive accumulation of inflammatory cells and well-preserved structures in the skin tissue of RT-01-treated mice compared with expressive infiltration of infected cells replacing the skin tissue in lesions of untreated mice. These findings highlight the fact that the apparent potency of organotellurane compounds, together with their relatively simple structure, may represent a new avenue for the development of novel drugs to combat parasitic diseases.

Citations

Citations to this article as recorded by

A blending-based therapeutic strategy using diaryl dichalcogenides and ivermectin against gastrointestinal nematodes of small ruminants
Irineu Romero-Neto, Júlia Dall’Anese, Tay Zugman, Eduarda Proença, Alda Lúcia Gomes Monteiro, Leandro Piovan, Marcelo Beltrão Molento
Veterinary Parasitology.2025; 338: 110529. CrossRef
Indazole Derivatives Against Murine Cutaneous Leishmaniasis
Niurka Mollineda-Diogo, Yunierkis Pérez-Castillo, Sergio Sifontes-Rodríguez, Osmani Marrero-Chang, Alfredo Meneses-Marcel, Alma Reyna Escalona-Montaño, María Magdalena Aguirre-García, Teresa Espinosa-Buitrago, Yeny Morales-Moreno, Vicente Arán-Redó
Pharmaceuticals.2025; 18(8): 1107. CrossRef
Analysis of thermomechanical coupled accelerated aging of HTPB propellants
Yi Zeng, Wei Huang, Jia‐Xing Chen, Jin‐Sheng Xu, Xiong Chen, Rui Wu, Qi‐Xuan Song
Propellants, Explosives, Pyrotechnics.2024;[Epub] CrossRef
Synthesis and application of organotellurium compounds
Biswa Mohan Sahoo, Bimal Krishna Banik, Abhishek Tiwari, Varsha Tiwari, Adya Jain, Preetismita Borah
Physical Sciences Reviews.2023; 8(12): 4435. CrossRef
Synthesis of Tellurium Oxide (TeO2) Nanorods and Nanoflakes and Evaluation of Its Efficacy Against Leishmania major In Vitro and In Vivo
Pooya Tavakoli, Fatemeh Ghaffarifar, Hamid Delavari, Amir KarimiPourSaryazdi, Mohammad Saaid Dayer, Vahid Nasiri, Salimeh Ahmadi
Acta Parasitologica.2022; 67(1): 143. CrossRef
Equilibrium between tri‐ and tetra‐coordinate chalcogenuranes is critical for cysteine protease inhibition
Gabriela Dias‐da‐Silva, Rodrigo L. O. R. Cunha, Mauricio D. Coutinho‐Neto
Journal of Computational Chemistry.2021; 42(17): 1225. CrossRef
Synthesis, Mechanism Elucidation and Biological Insights of Tellurium(IV)‐Containing Heterocycles
João Pedro A. Souza, Leociley R. A. Menezes, Francielle P. Garcia, Débora B. Scariot, Pamela T. Bandeira, Mateus B. Bespalhok, Siddhartha O. K. Giese, David L. Hughes, Celso V. Nakamura, Andersson Barison, Alfredo R. M. Oliveira, Renan B. Campos, Leandro
Chemistry – A European Journal.2021; 27(58): 14427. CrossRef
Stereoselective Bioreduction of Telluro‐Acetophenones to Optically Active Hydroxy Tellurides
Pamela Taisline Bandeira, Vicente Gotor‐Fernández, Leandro Piovan
European Journal of Organic Chemistry.2020; 2020(9): 1129. CrossRef
Tellurides Bearing Sulfonamides as Novel Inhibitors of Leishmanial Carbonic Anhydrase with Potent Antileishmanial Activity
Andrea Angeli, Mikel Etxebeste-Mitxeltorena, Carmen Sanmartín, Socorro Espuelas, Esther Moreno, Amaya Azqueta, Seppo Parkkila, Fabrizio Carta, Claudiu T. Supuran
Journal of Medicinal Chemistry.2020; 63(8): 4306. CrossRef
preADMET analysis and clinical aspects of dogs treated with the Organotellurium compound RF07: A possible control for canine visceral leishmaniasis?
Adriana Maria Viana Nunes, Francisco das Chagas Pereira de Andrade, Lívia Alves Filgueiras, Octávio Augusto de Carvalho Maia, Rodrigo L.O.R. Cunha, Sindy V.A. Rodezno, Antônio Luiz Martins Maia Filho, Fernando Aécio de Amorim Carvalho, Debora Cavalcante B
Environmental Toxicology and Pharmacology.2020; 80: 103470. CrossRef
Metal Compounds against Neglected Tropical Diseases
Yih Ching Ong, Saonli Roy, Philip C. Andrews, Gilles Gasser
Chemical Reviews.2019; 119(2): 730. CrossRef
Antitumor effect of chiral organotelluranes elicited in a murine melanoma model
Thaysa Paschoalin, Adam A. Martens, Álvaro T. Omori, Felipe V. Pereira, Luiz Juliano, Luiz R. Travassos, Glaucia M. Machado-Santelli, Rodrigo L.O.R. Cunha
Bioorganic & Medicinal Chemistry.2019; 27(12): 2537. CrossRef
Diacetal Ditellurides as Highly Active and Selective Antiparasitic Agents toward Leishmania amazonensis
Pamela T. Bandeira, João Pedro A. Souza, Débora B. Scariot, Francielle P. Garcia, Celso V. Nakamura, Alfredo R. M. de Oliveira, Leandro Piovan
ACS Medicinal Chemistry Letters.2019; 10(5): 806. CrossRef
Ammonium trichloro [1,2-ethanediolato-O,O′]-tellurate cures experimental visceral leishmaniasis by redox modulation of Leishmania donovani trypanothione reductase and inhibiting host integrin linked PI3K/Akt pathway
Preeti Vishwakarma, Naveen Parmar, Pragya Chandrakar, Tanuj Sharma, Manoj Kathuria, Pramod K. Agnihotri, Mohammad Imran Siddiqi, Kalyan Mitra, Susanta Kar
Cellular and Molecular Life Sciences.2018; 75(3): 563. CrossRef
Resuspendable Powders of Lyophilized Chalcogen Particles with Activity against Microorganisms
Sharoon Griffin, Muhammad Sarfraz, Steffen Hartmann, Shashank Pinnapireddy, Muhammad Nasim, Udo Bakowsky, Cornelia Keck, Claus Jacob
Antioxidants.2018; 7(2): 23. CrossRef
Synthesis, spectral characterization, DFT studies and biological activity of novel Ligand 1‐(2‐cyclohexyl thioethyl) piperidine and its complexes with group 12 metal chlorides
Muzzaffar A. Bhat, Shabir H. Lone, Muzzaffar A. Mir, Sheikh A. Majid, Sanjay K. Srivastava
Applied Organometallic Chemistry.2018;[Epub] CrossRef
Cysteine proteases in protozoan parasites
Jair L. Siqueira-Neto, Anjan Debnath, Laura-Isobel McCall, Jean A. Bernatchez, Momar Ndao, Sharon L. Reed, Philip J. Rosenthal, Photini Sinnis
PLOS Neglected Tropical Diseases.2018; 12(8): e0006512. CrossRef
Glucose-6-phosphate dehydrogenase and Trypanothione reductase interaction protects Leishmania donovani from metalloid mediated oxidative stress
Ayan Kumar Ghosh, Savita Saini, Sushmita Das, Abhishek Mandal, Abul Hasan Sardar, Md. Yousuf Ansari, Kumar Abhishek, Ajay Kumar, Ruby Singh, Sudha Verma, Asif Equbal, Vahab Ali, Pradeep Das
Free Radical Biology and Medicine.2017; 106: 10. CrossRef
Stability Study of Hypervalent Tellurium Compounds in Aqueous Solutions
Cleverson R. Princival, Marcos V. L. R. Archilha, Alcindo A. Dos Santos, Maurício P. Franco, Ataualpa A. C. Braga, André F. Rodrigues-Oliveira, Thiago C. Correra, Rodrigo L. O. R. Cunha, João V. Comasseto
ACS Omega.2017; 2(8): 4431. CrossRef
Thioridazine inhibits gene expression control of the cell wall signaling pathway (CWI) in the human pathogenic fungus Paracoccidioides brasiliensis
Daniela Leite Jabes, Ana Claudia de Freitas Oliveira, Valquíria Campos Alencar, Fabiano Bezerra Menegidio, Débora Liliane Souza Reno, Daiene Souza Santos, David Aciole Barbosa, Renata Ozelami Vilas Boas, Rodrigo Luiz de Oliveira Rodrigues Cunha, Tiago Rod
Molecular Genetics and Genomics.2016; 291(3): 1347. CrossRef
The Anticancer Activity of Organotelluranes: Potential Role in Integrin Inactivation
Alon Silberman, Yona Kalechman, Shira Hirsch, Ziv Erlich, Benjamin Sredni, Amnon Albeck
ChemBioChem.2016; 17(10): 918. CrossRef
Synthesis and Characterization of 2-Fluoro-3-Pyridyl Tellurium Compounds: X-Ray Crystal Structure of Bis(2-Fluoro-3-Pyridyltelluro)Methane
Shivani Gulati (nee Doomra), Shikha Pundir, Sanjeev Kumar, Kuldip K. Bhasin
Phosphorus, Sulfur, and Silicon and the Related Elements.2015; 190(9): 1509. CrossRef
Evaluation of the leishmanicidal and cytotoxic effects of inhibitors for microorganism metabolic pathway enzymes
Ademar de Mesquita Barbosa, Solange dos Santos Costa, Josmar Rodrigues da Rocha, Carlos Alberto Montanari, Selma Giorgio
Biomedicine & Pharmacotherapy.2015; 74: 95. CrossRef
Synthesis, antimicrobial activity, computational and modeling studies of some new organotellurium compounds containing azo moities
Wasfi Aboud Al-Masoudi, Rafid Hmedan Al-Asadi, Rasha Munther Othman, Najim Aboud Al‐Masoudi
European Journal of Chemistry.2015; 6(4): 374. CrossRef
Antileishmanial activity of essential oil from Chenopodium ambrosioides and its main components against experimental cutaneous leishmaniasis in BALB/c mice
L. Monzote, J. Pastor, R. Scull, L. Gille
Phytomedicine.2014; 21(8-9): 1048. CrossRef
Triphenyltellurium chloride
Ambika Chopra, Shalini Jain, Sanjay K. Srivastava, Sushil K. Gupta, Ray J. Butcher
Acta Crystallographica Section E Structure Reports Online.2014; 70(4): o421. CrossRef
Recent Developments in Drug Discovery for Leishmaniasis and Human African Trypanosomiasis
Advait S. Nagle, Shilpi Khare, Arun Babu Kumar, Frantisek Supek, Andriy Buchynskyy, Casey J. N. Mathison, Naveen Kumar Chennamaneni, Nagendar Pendem, Frederick S. Buckner, Michael H. Gelb, Valentina Molteni
Chemical Reviews.2014; 114(22): 11305. CrossRef
Oxidative stress in mice treated with antileishmanial meglumine antimoniate
D.B. Bento, B. de Souza, A.V. Steckert, R.O. Dias, D.D. Leffa, S.E. Moreno, F. Petronilho, V.M. de Andrade, F. Dal-Pizzol, P.R. Romão
Research in Veterinary Science.2013; 95(3): 1134. CrossRef
Therapeutic potential of selenium and tellurium compounds: Opportunities yet unrealised
Edward R. T. Tiekink
Dalton Transactions.2012; 41(21): 6390. CrossRef
In Vitro and In Vivo Activity of an Organic Tellurium Compound on Leishmania (Leishmania) chagasi
Isabella Aparecida Salerno Pimentel, Carolina de Siqueira Paladi, Simone Katz, Wagner Alves de Souza Júdice, Rodrigo L. O. R. Cunha, Clara Lúcia Barbiéri, Dan Zilberstein
PLoS ONE.2012; 7(11): e48780. CrossRef
Bis(μ-phenyltellurido-κ2Te:Te)bis[tetracarbonylrhenium(I)]
J. Muthukumaran, M. Kannan, A. Vanitha, Bala Manimaran, R. Krishna
Acta Crystallographica Section E Structure Reports Online.2010; 66(5): m558. CrossRef
Heptacarbonyl-1κ3C,2κ4C-(4-phenylpyridine-1κN)di-μ-phenyltellurido-1:2κ4Te:Te-dirhenium(I)
A. Vanitha, J. Muthukumaran, R. Krishna, Bala Manimaran
Acta Crystallographica Section E Structure Reports Online.2010; 66(5): m518. CrossRef