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"Toxocariasis"

Brief Communication

Organ-specific Toxocara canis larvae migration and host immune response in experimentally infected mice
Min Seok Kim, Yan Jin, Se Joon Woo
Parasites Hosts Dis 2024;62(2):243-250.
Published online May 27, 2024
DOI: https://doi.org/10.3347/PHD.23125
We investigated organ specific Toxocara canis larval migration in mice infected with T. canis larvae. We observed the worm burden and systemic immune responses. Three groups of BALB/c mice (n=5 each) were orally administered 1,000 T. canis 2nd stage larvae to induce larva migrans. Mice were sacrificed at 1, 3, and 5 weeks post-infection. Liver, lung, brain, and eye tissues were collected. Tissue from 2 mice per group was digested for larval count, while the remaining 3 mice underwent histological analysis. Blood hematology and serology were evaluated and compared to that in a control uninfected group (n=5) to assess the immune response. Cytokine levels in bronchoalveolar lavage (BAL) fluid were also analyzed. We found that, 1 week post-infection, the mean parasite load in the liver (72±7.1), brain (31±4.2), lungs (20±5.7), and eyes (2±0) peaked and stayed constant until the 3 weeks. By 5-week post-infection, the worm burden in the liver and lungs significantly decreased to 10±4.2 and 9±5.7, respectively, while they remained relatively stable in the brain and eyes (18±4.2 and 1±0, respectively). Interestingly, ocular larvae resided in all retinal layers, without notable inflammation in outer retina. Mice infected with T. canis exhibited elevated levels of neutrophils, monocytes, eosinophils, and immunoglobulin E. At 5 weeks post-infection, interleukin (IL)-5 and IL-13 levels were elevated in BAL fluid. Whereas IL-4, IL-10, IL-17, and interferon-γ levels in BAL fluid were similar to that in controls. Our findings demonstrate that a small portion of T. canis larvae migrate to the eyes and brain within the first week of infection. Minimal tissue inflammation was observed, probably due to increase of anti-inflammatory cytokines. This study contributes to our understanding of the histological and immunological responses to T. canis infection in mice, which may have implications to further understand human toxocariasis.

Citations

Citations to this article as recorded by  Crossref logo
  • Toxocara canis infections in mice: from subtle to severe consequences in 100 weeks
    Nicol Bernardová, Jan Novák, Chia-Kwung Fan, Libuše Kolářová, Marta Chanová
    Journal of Helminthology.2025;[Epub]     CrossRef
  • The association of seropositivity to Toxocara canis and Ascaris lumbricoides with blood group antigens
    Kravchun Pavlo Grigorovich, Leontyeva Frida Solomonivna, Povelichenko Olena Dmytrivna, Kadykova Olga Igorivna, Valentyna Dielievska
    Reviews and Research in Medical Microbiology.2025;[Epub]     CrossRef
  • Characteristics of Toxocara canis induced lung inflammation in C57BL/6 mice
    Janina Lekki-Jóźwiak, Justyna Karabowicz, Magdalena Paschall, Karolina Gregorczyk-Zboroch, Małgorzata Sobczak-Filipiak, Piotr Bąska, Irma Schabussova, Ewa Długosz
    Frontiers in Immunology.2025;[Epub]     CrossRef
  • Immunomodulatory and anti-fibrotic effects of Toxocara canis infection in a murine model of thioacetamide-induced chronic hepatic fibrosis
    Iman F. Abou-El-Naga, Eman Dorry Elkerdany, Rania G. Aly, Enas Mohamed Mostafa Zaytoun
    Acta Tropica.2025; 272: 107913.     CrossRef
  • 5,150 View
  • 87 Download
  • 4 Web of Science
  • Crossref

Case Report

A Case Report of Intravitreal Dexamethasone Implant with Exudative Retinal Detachment for Ocular Toxocariasis Treatment
Youran Cai, Ye Yang, Xin Zhong
Korean J Parasitol 2022;60(2):133-137.
Published online April 20, 2022
DOI: https://doi.org/10.3347/kjp.2022.60.2.133
Toxocariasis is one of the most common geohelminth infections in several parts of the world. We describe a rare case of ocular toxocariasis with secondary exudative retinal detachment treated with albendazole and an intravitreal dexamethasone implant. A 13-year-old boy with counting finger vision was diagnosed with retinal vasculitis and exudative retinal detachment in his right eye. Fundoscopic examination revealed retinal hemorrhage, retinal vasculitis, and exudative retinal detachment. Serological test using serum and intraocular aqueous humor were positive for anti-Toxocara specific IgG antibodies. He received repeated doses of intravitreal dexamethasone implants combined with oral albendazole. A sequential follow-up optical coherence tomography revealed that the retina was successfully reattached. His visual acuity subsequently improved to 20/400.

Citations

Citations to this article as recorded by  Crossref logo
  • Human toxocariasis
    Susana Lopez-Alamillo, Pravallika Padyala, Megan Carey, Megan M. Duffey, Jill E. Weatherhead, Louisa A. Messenger, Bachir Medrouh, Marcelo U. Ferreira
    Clinical Microbiology Reviews.2025;[Epub]     CrossRef
  • Intravitreal Dexamethasone Implant (Ozurdex) for Ocular Toxocariasis
    Yongwei Zhou, Fangyuan Zhen, Jiahui Wu, Shasha Wang, Xiaoyan Lu, Ge Yang, Zhirou Hu, Fei Chen, Qiuming Li, Shuqian Dong, Akio Oishi
    Journal of Ophthalmology.2024;[Epub]     CrossRef
  • The application of dexamethasone implants in uveitis treatment
    Tian Zhang, Zhutao Liu, Na Li
    Frontiers in Medicine.2024;[Epub]     CrossRef
  • Case report: Ultrasound biomicroscopy as a guide for the selection of injection sites for dexamethasone intravitreal implant (Ozurdex) for peripheral granulomatous ocular toxocariasis in children
    Xin Zhang, Xinzhu Hou, Yan Zhang, Jingjie Liu, Zhiyong Zhang
    Frontiers in Medicine.2023;[Epub]     CrossRef
  • 4,723 View
  • 188 Download
  • 4 Web of Science
  • Crossref

Original Articles

Prevalence of Toxocariasis and Its Risk Factors in Patients with Eosinophilia in Korea
Hyun Beom Song, Deokho Lee, Yan Jin, Jinwoo Kang, Shin-Hyeong Cho, Min Sun Park, Jin-Ho Park, Woo-Jung Song, Hye-Ryun Kang, Sang Hyub Lee, Sung-Tae Hong, Min-Ho Choi
Korean J Parasitol 2020;58(4):413-419.
Published online August 26, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.4.413
Eosinophilia occurs commonly in many diseases including allergic diseases and helminthic infections. Toxocariasis has been suggested as one cause of eosinophilia. The present study was undertaken to examine the prevalence of toxocariasis in patients with eosinophilia and to identify the risk factors for toxocariasis. This prospective cohort study recruited a total of 81 patients with eosinophilia (34 males and 47 females) who visited the outpatient clinic at Seoul National University Hospital from January 2017 to February 2018 and agreed to participate in this study. The prevalence of toxocariasis was examined by T. canis-specific ELISA, and the various risk factors for toxocariasis were evaluated by a questionnaire survey. Among 81 patients with eosinophilia, 18 were positive for anti-T. canis antibodies (22.2%); 88.9% were male (16/18) and 11.1% were female (2/18). Multivariate statistical analysis revealed that males (OR 21.876, 95% CI: 1.667-287.144) with a history of consuming the raw meat or livers of animals (OR 5.899, 95% CI: 1.004-34.669) and a heavy alcohol-drinking habit (OR 8.767, 95% CI: 1.018-75.497) were at higher risk of toxocariasis in patients with eosinophilia. Toxocariasis should be considered a potential cause of eosinophilia when the patient has a history of eating the raw meat or livers of animals in Korea. A single course of albendazole is recommended to reduce the migration of Toxocara larvae in serologically positive cases with eosinophilia.

Citations

Citations to this article as recorded by  Crossref logo
  • Evaluation of a Multiplex Bead Assay for Strongyloides stercoralis diagnosis using the recombinant antigen rSs-NIE-1
    Joelma Nascimento de Souza, Wéslei Almeida Costa Araújo, Márcia Cristina Aquino Teixeira, Neci Matos Soares
    Diagnostic Microbiology and Infectious Disease.2026; 114(2): 117108.     CrossRef
  • High toxocariasis seroprevalence in a tri-border indigenous community (Brazil, Paraguay and Argentina): A One Health perspective
    Isabella Braghin Ferreira, Roberto Teixeira de Souza Filho, Susana Angélica Zevallos Lescano, Rogério Giuffrida, Daniele Rodrigues, Suelen Teixeira de Faria Resende, Fabiano Borges Figueiredo, Louise Bach Kmetiuk, Andrea Pires dos Santos, Alexander Welker
    One Health.2025; 21: 101106.     CrossRef
  • Human toxocariasis
    Susana Lopez-Alamillo, Pravallika Padyala, Megan Carey, Megan M. Duffey, Jill E. Weatherhead, Louisa A. Messenger, Bachir Medrouh, Marcelo U. Ferreira
    Clinical Microbiology Reviews.2025;[Epub]     CrossRef
  • First Report on the Seroprevalence and Risk Factors Associated with Toxocara Infection in Blood Donors from Romania
    Ana Alexandra Ardelean, Rodica Lighezan, Sorin Ursoniu, Sergiu Adrian Sprintar, Daniela Adriana Oatis, Alin Gabriel Mihu, Maria Alina Lupu, Tudor Rareș Olariu
    Pathogens.2025; 14(9): 857.     CrossRef
  • TỶ LỆ VÀ YẾU TỐ LIÊN QUAN NHIỄM TOXOCARA CANIS TẠI PHÒNG KHÁM BỆNH VIỆN ĐẠI HỌC Y DƯỢC 1 THÀNH PHỐ HỒ CHÍ MINH
    Nguyễn Ngọc Phi Anh Nguyễn Ngọc Phi Anh, Trần Thị Kim Dung Trần Thị Kim Dung, Huỳnh Thị Thu Thảo Huỳnh Thị Thu Thảo
    Tạp Chí Khoa Học Trường Đại Học Quốc Tế Hồng Bàng.2024; : 213.     CrossRef
  • Toxocariasis: potential association with bronchial asthma, and pneumonia among pediatric children
    Wegdan M. Abd El Wahab, Mona I. Ali, Shimaa S. Ibrahim, Yasmen A. Mohamed, Doaa A. Hamdy
    Journal of Parasitic Diseases.2023; 47(1): 93.     CrossRef
  • One health approach to toxocariasis in quilombola communities of southern Brazil
    Vamilton Alvares Santarém, Giovanni Kalempa Panazzolo, Louise Bach Kmetiuk, Orlei José Domingues, Isabella Braghin Ferreira, Roberto Teixeira de Souza Filho, João Henrique Farinhas, Fernando Rodrigo Doline, Susana Angélica Zevallos Lescano, Leandro Menegu
    Parasites & Vectors.2023;[Epub]     CrossRef
  • Seroprevalence of toxocariasis among allergic patients in Kuwait and its association with eosinophilia
    Mohammad Al-Awadhi, Wafaa Jamal
    Parasite Epidemiology and Control.2022; 18: e00260.     CrossRef
  • Green vegetable juice as a potential source of human fascioliasis in Korea
    Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
    One Health.2022; 15: 100441.     CrossRef
  • Serosurvey of anti-Toxocara canis antibodies in people experiencing homelessness and shelter workers from São Paulo, Brazil
    Vamilton Alvares Santarém, Anahi Chechia do Couto, Susana Zevallos Lescano, William Henry Roldán, Ruana Renostro Delai, Rogério Giuffrida, Louise Bach Kmetiuk, Alexander Welker Biondo, Sriveny Dangoudoubiyam, Andrea Pires dos Santos
    Parasites & Vectors.2022;[Epub]     CrossRef
  • Seroprevalence of Toxocariasis Among Hypereosinophilic Children: A Single Center Study, Tehran, Iran
    Meysam Sharifdini, Mehdi Zarean, Mehrzad Sadredinamin, Mitra Zare- Bavani, Sarah Sharifi-yazdi, Bahram Nikmanesh
    Archives of Pediatric Infectious Diseases.2022;[Epub]     CrossRef
  • The Role of Nile Tilapia (Oreochromis niloticus) in the Life Cycle of Toxocara spp.
    Everton André de Oliveira, Yslla Fernanda Fitz Balo Merigueti, Isabella Braghin Ferreira, Isabele Santos Garcia, Alini Soriano Pereira, Rosemeire de Souza Santos, Louise Bach Kmetiuk, Andrea Pires dos Santos, Alexander Welker Biondo, Rogerio Giuffrida, Va
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • 7,306 View
  • 185 Download
  • 11 Web of Science
  • Crossref
Immunopathological Changes in the Brain of Immunosuppressed Mice Experimentally Infected with Toxocara canis
Mohamed M. Eid, Samy I. El-Kowrany, Ahmad A. Othman, Dina I. El Gendy, Eman M. Saied
Korean J Parasitol 2015;53(1):51-58.
Published online February 27, 2015
DOI: https://doi.org/10.3347/kjp.2015.53.1.51

Toxocariasis is a soil-transmitted helminthozoonosis due to infection of humans by larvae of Toxocara canis. The disease could produce cognitive and behavioral disturbances especially in children. Meanwhile, in our modern era, the incidence of immunosuppression has been progressively increasing due to increased incidence of malignancy as well as increased use of immunosuppressive agents. The present study aimed at comparing some of the pathological and immunological alterations in the brain of normal and immunosuppressed mice experimentally infected with T. canis. Therefore, 180 Swiss albino mice were divided into 4 groups including normal (control) group, immunocompetent T. canis-infected group, immunosuppressed group (control), and immunosuppressed infected group. Infected mice were subjected to larval counts in the brain, and the brains from all mice were assessed for histopathological changes, astrogliosis, and IL-5 mRNA expression levels in brain tissues. The results showed that under immunosuppression, there were significant increase in brain larval counts, significant enhancement of reactive gliosis, and significant reduction in IL-5 mRNA expression. All these changes were maximal in the chronic stage of infection. In conclusion, the immunopathological alterations in the brains of infected animals were progressive over time, and were exaggerated under the effect of immunosuppression as did the intensity of cerebral infection.

Citations

Citations to this article as recorded by  Crossref logo
  • Toxocara canis infections in mice: from subtle to severe consequences in 100 weeks
    Nicol Bernardová, Jan Novák, Chia-Kwung Fan, Libuše Kolářová, Marta Chanová
    Journal of Helminthology.2025;[Epub]     CrossRef
  • Using Biocontrol Fungi to Control Helminthosis in Wild Animals: An Innovative Proposal for the Health and Conservation of Species
    Júlia dos Santos Fonseca, Beatriz Bacelar Barbosa, Adolfo Paz Silva, María Sol Arias Vázquez, Cristiana Filipa Cazapal Monteiro, Huarrisson Azevedo Santos, Jackson Victor de Araújo
    Pathogens.2025; 14(8): 775.     CrossRef
  • The potential relationship between Toxocara canis infection and epilepsy in a rat model
    Amina M. Salama, Dina I. Elgendy, Rasha A. Elmahy, Asmaa F. Eltantawy, Monira A. Seleem, Ahmed M. Elgohary, Omaima K. Docmac, Marwa E. F. Al Mwafy, Ahmed A. Almeldin, Mahmoud S. Sharaf
    Parasitology Research.2025;[Epub]     CrossRef
  • Efficacy of artemether against toxocariasis in mice: parasitological and immunopathological changes in brain, liver, and lung
    Dina I. Elgendy, Rasha A. Elmahy, Alaa Ibrahim Mohamed Amer, Hoda A. Ibrahim, Asmaa Fawzy Eltantawy, Fotouh Rashed Mansour, Amina M. Salama
    Pathogens and Global Health.2024; 118(1): 47.     CrossRef
  • Neurobehavioral Disorders and Pathological Changes in the Brain of Mice Are Caused by Chronic Toxocara canis Larval Invasion with Low to Moderate Inoculum
    Nicol Bernardová, Jan Novák, Petr Horák, Chia-Kwung Fan, Libuše Kolářová
    Acta Parasitologica.2024; 69(4): 1736.     CrossRef
  • Effects of metformin on parasitological, pathological changes in the brain and liver and immunological aspects during visceral toxocariasis in mice
    Amina M. Salama, Rasha A. Elmahy, Hoda A. Ibrahim, Alaa Ibrahim Mohamed Amer, Asmaa Fawzy Eltantawy, Dina I. Elgendy
    Parasitology Research.2023; 122(12): 3213.     CrossRef
  • Cyclosporine A increases the intensity of Toxocara canis infection in swiss mice
    W. D. S. Terto, M. Q. de Moura, J. L. Borchardt, F. D. S. Santos, L. F. da Costa Avila, N. B. Pinheiro, F. P. Leivas Leite, M. M. Villela, M. E. A. Berne
    Brazilian Journal of Biology.2022;[Epub]     CrossRef
  • Glial Fibrillary Acidic Protein and Ionized Calcium-Binding Adapter Molecule 1 Immunostaining Score for the Central Nervous System of Horses With Non-suppurative Encephalitis and Encephalopathies
    Gisele Silva Boos, Klaus Failing, Edson Moleta Colodel, David Driemeier, Márcio Botelho de Castro, Daniele Mariath Bassuino, José Diomedes Barbosa, Christiane Herden
    Frontiers in Veterinary Science.2021;[Epub]     CrossRef
  • Diallyl sulfide alleviates cyclophosphamide-induced nephropathic encephalopathy in rats
    Shereen M. Galal, Heba H. Mansour, Abeer A. Elkhoely
    Toxicology Mechanisms and Methods.2020; 30(3): 208.     CrossRef
  • Mesenchymal stem cells combined with albendazole as a novel therapeutic approach for experimental neurotoxocariasis
    E. V. N. Beshay, S. A. El-Refai, G. S. Sadek, A. A. Elbadry, F. H. Shalan, A. F. Afifi
    Parasitology.2020; 147(7): 799.     CrossRef
  • Histopathological characterization of Toxocara canis- and T. cati-induced neurotoxocarosis in the mouse model
    Andrea Springer, Lea Heuer, Elisabeth Janecek-Erfurth, Andreas Beineke, Christina Strube
    Parasitology Research.2019; 118(9): 2591.     CrossRef
  • Developmental stages and viability of Toxocara canis eggs outside the host
    Iman Fathy Abou-El-Naga
    Biomédica.2018; 38(2): 189.     CrossRef
  • Seroprevalence and Risk Factors for Toxocara canis Infection in Serbia During 2015
    Simona Gabrielli, Suzana Tasić-Otašević, Aleksandra Ignjatović, Maurizio Fraulo, Marija Trenkić-Božinović, Stefan Momčilović, Gabriella Cancrini
    Foodborne Pathogens and Disease.2017; 14(1): 43.     CrossRef
  • 12,708 View
  • 160 Download
  • 16 Web of Science
  • Crossref

Case Reports

A Case of Ocular Toxocariasis Successfully Treated with Albendazole and Triamcinolon
San Seong, Daruchi Moon, Dong Kyu Lee, Hyung Eun Kim, Hyun Sup Oh, Soon Hyun Kim, Oh Woong Kwon, Yong Sung You
Korean J Parasitol 2014;52(5):537-540.
Published online October 22, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.5.537

We present a case of ocular toxocariasis treated successfully with oral albendazole in combination with steroids. A 26-year-old male visited the authors' clinic with the chief complaint of flying flies in his right eye. The fundus photograph showed a whitish epiretinal scar, and the fluorescein angiography revealed a hypofluorescein lesion of the scar and late leakage at the margin. An elevated retinal surface and posterior acoustic shadowing of the scar were observed in the optical coherence tomography, and Toxocara IgG was positive. The patient was diagnosed with toxocariasis, and the condition was treated with albendazole (400 mg twice a day) for a month and oral triamcinolone (16 mg for 2 weeks, once a day, and then 8 mg for 1 week, once a day) from day 13 of the albendazole treatment. The lesions decreased after the treatment. Based on this study, oral albendazole combined with steroids can be a simple and effective regimen for treating ocular toxocariasis.

Citations

Citations to this article as recorded by  Crossref logo
  • Who Let the Dogs Out? Unmasking the Neglected: A Semi-Systematic Review on the Enduring Impact of Toxocariasis, a Prevalent Zoonotic Infection
    Katrin Henke, Sotirios Ntovas, Eleni Xourgia, Aristomenis K. Exadaktylos, Jolanta Klukowska-Rötzler, Mairi Ziaka
    International Journal of Environmental Research and Public Health.2023; 20(21): 6972.     CrossRef
  • Toxocariasis ocular simulando un hamartoma retiniano. Reportefide un caso
    Luis R. Michel-Espinoza, Belina Arias-Cabello, Daniel Moreno-Páramo, M. Estela Arroyo-Yllanes
    Revista Mexicana de Oftalmología.2021; 95(6S): 285.     CrossRef
  • Treatment of larva migrans syndrome with long-term administration of albendazole
    Amy Hombu, Ayako Yoshida, Taisei Kikuchi, Eiji Nagayasu, Mika Kuroki, Haruhiko Maruyama
    Journal of Microbiology, Immunology and Infection.2019; 52(1): 100.     CrossRef
  • Ocular toxocariasis in a pediatric patient undergoing a bone marrow transplantation
    Blanca Molina, Marta González-Vicent, Isabel Valls, Miguel Ángel Díaz
    Enfermedades Infecciosas y Microbiología Clínica.2019; 37(9): 617.     CrossRef
  • Ocular toxocariasis: a neglected parasitic disease in Egypt
    Nagwa Mostafa El-Sayed, Nagham Gamal Masoud
    Bulletin of the National Research Centre.2019;[Epub]     CrossRef
  • Ocular toxocariasis in a pediatric patient undergoing a bone marrow transplantation
    Blanca Molina, Marta González-Vicent, Isabel Valls, Miguel Ángel Díaz
    Enfermedades infecciosas y microbiologia clinica (English ed.).2019; 37(9): 618.     CrossRef
  • Toxocariasis: critical analysis of serology in patients attending a public referral center for ophthalmology in Brazil
    Guita Rubinsky-Elefant, Joyce H. Yamamoto, Carlos E. Hirata, Luiz E. Prestes-Carneiro
    Japanese Journal of Ophthalmology.2018; 62(1): 77.     CrossRef
  • 13,223 View
  • 124 Download
  • 5 Web of Science
  • Crossref
Pulmonary Toxocariasis Mimicking Invasive Aspergillosis in a Patient with Ulcerative Colitis
Eun Jin Park, Joon Young Song, Min Ju Choi, Ji Ho Jeon, Jah-yeon Choi, Tae Un Yang, Kyung Wook Hong, Ji Yun Noh, Hee Jin Cheong, Woo Joo Kim
Korean J Parasitol 2014;52(4):425-428.
Published online August 29, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.4.425

A 45-year-old-male who had underlying ulcerative colitis and presented with fever and dry cough. Initially, the patient was considered to have invasive aspergillosis due to a positive galactomannan assay. He was treated with amphotericin B followed by voriconazole. Nevertheless, the patient deteriorated clinically and radiographically. The lung biopsy revealed eosinophilic pneumonia, and ELISA for Toxocara antigen was positive, leading to a diagnosis of pulmonary toxocariasis. After a 10-day treatment course with albendazole and adjunctive steroids, the patient recovered completely without any sequelae. Pulmonary toxocariasis may be considered in patients with subacute or chronic pneumonia unresponsive to antibiotic agents, particularly in cases with eosinophilia.

  • 11,705 View
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Original Article

Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
Yan Jin, Chenghua Shen, Sun Huh, Woon-Mok Sohn, Min-Ho Choi, Sung-Tae Hong
Korean J Parasitol 2013;51(4):433-439.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.433

Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clinically confirmed toxocariasis, 115 healthy controls, and 119 other tissue-invading helminthiases were screened by ELISA using TCLA. The ELISA using TCLA showed 92.2% (59/64 patient samples) sensitivity and 86.6% (103/119) specificity. Its positive diagnostic predictivity was 78.7% and negative predictivity was 97.8%. No serum of healthy controls reacted but that of anisakiasis (45.5%), gnathostomiasis (19.2%), clonorchiasis (15.8%), sparganosis (11.1%), and cysticercosis (6.3%) cross-reacted. Immunoblot analysis on TCLA recognized antigenic proteins of 28- and 30-kDa bands in their dominant protein quantity and strong blotting reactivity. The present results indicate that the ELISA using our TCLA antigen is acceptable by the sensitivity and specificity for serodiagnosis of human toxocariasis. ELISA with TCLA is recommended to make differential diagnosis for patients with any sign of organ infiltration and eosinophilia.

Citations

Citations to this article as recorded by  Crossref logo
  • Intermediate Uveitis: An Updated Review of the Differential Diagnosis and Relevant Special Investigations
    Jacob Biju Mark, Derrick P. Smit, Rajashree E, Rathinam SR
    Ocular Immunology and Inflammation.2025; 33(4): 522.     CrossRef
  • Análisis serológico de Toxocara canis y Toxocara cati por ELISA en niños de una escuela de educación básica de la provincia de Chimborazo
    Laura Katheryne Hernández León, Sandra Noemí Escobar Arrieta, Verónica Mercedes Cando Brito
    Anatomía Digital.2025; 8(2.2): 93.     CrossRef
  • Epidemiological profile of human toxocariasis in patients examined at Evandro Chagas Institute (IEC/SVSA/MS) between 2014 and 2019
    Lucas Solano Araújo da Silva, Isabelle Helena Lima Dias, Álvaro Luan Santana Fonseca, Martin Johannes Enk, Joyce Favacho Cardoso Nogueira, Ricardo José de Paula Souza e Guimarães, Christiane de Oliveira Goveia
    Comparative Immunology, Microbiology and Infectious Diseases.2024; 105: 102112.     CrossRef
  • Evaluation of Somatic Antigens of Adult Toxocara helminthes for Detection of Human Toxocariasis
    Zahra Navi, Reza Falak, Mehdi Mohebali, Mohammad Bagher Molairad, Zabihollah Zarei, Mojgan Aryaeipour, Abbas Rahimi Foroushani, Mohammad Zibaei, Mohammad Bagher Rokni
    Parasite Immunology.2024;[Epub]     CrossRef
  • Toxocara canis and Fasciola hepatica Co-Infection Leading to Hepatic Abscess: A Case Report
    Seung Wan Kim, Byoung Kuk Jang
    Journal of Korean Medical Science.2023;[Epub]     CrossRef
  • Who Let the Dogs Out? Unmasking the Neglected: A Semi-Systematic Review on the Enduring Impact of Toxocariasis, a Prevalent Zoonotic Infection
    Katrin Henke, Sotirios Ntovas, Eleni Xourgia, Aristomenis K. Exadaktylos, Jolanta Klukowska-Rötzler, Mairi Ziaka
    International Journal of Environmental Research and Public Health.2023; 20(21): 6972.     CrossRef
  • Eosinophilic leukaemoid reaction and myocardial involvement in a male adolescent with Toxocara canis infection
    Panagiotis Krepis, Nikos Spyridis, Despina N Maritsi, Vasiliki Tsekoura, Lydia Kossiva
    Journal of Paediatrics and Child Health.2021; 57(6): 935.     CrossRef
  • A new ELISA and western blot technique based on recombinant TES antigen and/or larval antigen for the detection of toxocariasis in humans
    Marie-Kristin Raulf, Daniela Jordan, Herbert Auer, Jens M. Warnecke, Bernd Lepenies, Christina Strube
    Parasitology.2021; 148(3): 333.     CrossRef
  • Screening of Cystic Echinococcosis and Toxocariasis in Urmia Municipal Workers, Northwest Iran
    Negar Asadi, Khosrow Hazrati Tappeh, Iraj Mohebbi, Elham Yousefi, Shahram Khademvatan
    Infectious Disorders - Drug Targets.2021; 21(2): 220.     CrossRef
  • A Neurotoxocariasis Case Manifesting Multiple Cerebral Infarction and Eosinophilic Meningoencephalitis
    SangJoon Kang, Jaeyoung Park, Hoe Jong Jeong, Jae-Jeong Joo, Seungmin Kim
    Journal of the Korean Neurological Association.2021; 39(4): 331.     CrossRef
  • Toxocariasis as a Rare Parasitic Complication of a Transthoracic Spine Surgery Procedure
    Jan Soukup, Jan Cerny, Martin Cegan, Petr Kelbich, Tomas Novotny
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Brief Communication

A Seroepidemiological Survey of Toxocariasis among Eosinophilia Patients in Chungcheongnam-do
Min Seo, Sung Chul Yoon
Korean J Parasitol 2012;50(3):249-251.
Published online August 13, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.3.249

There have been several reports on the relationship between toxocariasis and eosinophilia, but all have been limited to the areas of Seoul or Gangwon-do. In the present study, we investigated the seroprevalence of toxocariasis among eosinophilia patients in Chungcheongnam-do, the central district of Korea. Among the 101 patients tested, 51 (50.5%) were identified as positive by Toxocara ELISA, and 46 (45.5%) were confidently diagnosed with toxocariasis because of absence of any other cause of eosinophilia. Whereas 22 of 42 seropositive patients (52.3%) had a recent history of consuming raw livers, especially the cow liver, only 1 of 25 seronegative patients (4%) had done so (P<0.01). From these results, we could confirm that toxocariasis is related to eosinophilia, and infer that ingestion of raw cow liver plays a vital role in the transmission of toxocariasis in Chungcheongnam-do.

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Original Articles

Transmission of Toxocara canis via Ingestion of Raw Cow Liver: A Cross-Sectional Study in Healthy Adults
Dongil Choi, Jae Hoon Lim, Dong-Chull Choi, Kyung Soo Lee, Seung Woon Paik, Sun-Hee Kim, Yoon-Ho Choi, Sun Huh
Korean J Parasitol 2012;50(1):23-27.
Published online March 6, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.1.23

The aim of this study is to ascertain the relationship between ingestion of raw cow liver and Toxocara canis infection. A total of 150 apparently healthy adults were divided into 2 groups; 1 group consisted of 86 adults with positive results of Toxocara ELISA, and the other group of 64 adults with negative results. One researcher collected the history of ingestion of raw cow liver within 1 year and recent history of keeping dogs. Among 86 seropositive adults for T. canis, 68 (79.1%) had a recent history of ingestion of raw cow liver. Multivariate statistical analysis showed that a recent ingestion of raw cow liver and keeping dogs were related to an increased risk of toxocariasis (odds ratios, 4.4 and 3.7; and 95% confidence intervals, 1.9-10.2 and 1.2-11.6, respectively). A recent history of ingestion of raw cow liver and keeping dogs was significantly associated with toxocariasis.

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Toxocariasis and Ingestion of Raw Cow Liver in Patients with Eosinophilia
Dongil Choi, Jae Hoon Lim, Dong-Chull Choi, Seung Woon Paik, Sun-Hee Kim, Sun Huh
Korean J Parasitol 2008;46(3):139-143.
Published online September 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.3.139

Ingestion of raw animal liver has been suggested as a possible mode of infection of human toxocariasis. We evaluated the relationship between toxocariasis and the ingestion of raw meat in patients with eosinophilia of unknown etiology. The study population consisted of 120 patients presenting with peripheral blood eosinophilia (> 500 cells/?l or > 10% of the white blood cell count). They were divided into 2 groups: 104 seropositive patients based on a Toxocara excretory-secretory IgG ELISA and 16 seronegative patients. While 25.0% of seronegative patients had a recent history of eating raw cow liver, 87.5% of seropositive patients had this history. Multivariate statistical analysis showed that a recent history of eating raw cow liver was related to an increased risk of toxocariasis. Collectively, it is proposed that raw cow liver is a significant infection source of toxocariasis in the patients with eosinophilia of unknown etiology.

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Seroprevalence of Toxocariasis among Healthy People with Eosinophilia
Yong-Hun Kim, Sun Huh, Young-Bae Chung
Korean J Parasitol 2008;46(1):29-32.
Published online March 20, 2008
DOI: https://doi.org/10.3347/kjp.2008.46.1.29

The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.

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Brief Communications

The intestines and hearts of dogs were examined for Toxocara canis, Toxascaris leonina, and Dirofilaria immitis, after necropsy between June 26 and September 29, 2004 in Chuncheon, Korea. Of the 662 dogs examined, 6 were infected with T. canis (0.9%), 86 with T. leonina (13.0%). Fifty dogs were infected with D. immitis among 500 dogs examined (10.0%). Five were co-infected with T. canis and T. leonina, and three were co-infected with T. leonina and D. immitis. The cumulative positive infection rate for three species was 134/662(20.2%). Considering previously reported seropositive rates of T. canis excretory-secretory antigen, i.e., 5% in the adult population in Korea, the possibility of toxocariasis caused by T. leonina should be reevaluated.

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  • Significance of Serum Antibody Test for Toxocariasis in Healthy Healthcare Examinees with Eosinophilia in Seoul and Gyeongsangnam-do, Korea
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    Min Seo, Sung Chul Yoon
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  • Seroprevalence of Toxocariasis among Healthy People with Eosinophilia
    Yong-Hun Kim, Sun Huh, Young-Bae Chung
    The Korean Journal of Parasitology.2008; 46(1): 29.     CrossRef
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Seroprevalence of Toxocara antibodies among patients suspected of ocular toxocariasis in Slovenia
Jernej Logar, Barbara ?oba, Aleksandra Kraut, Branka Stirn-Kranjc
Korean J Parasitol 2004;42(3):137-140.
Published online September 20, 2004
DOI: https://doi.org/10.3347/kjp.2004.42.3.137

Ocular toxocariasis named also ocular larva migrans is caused by larvae of the roundworm Toxocara spp. The purpose of this study was to find out the seroprevalence of Toxocara antibodies in patients suspected of ocular toxocariasis. Between January 2001 and December 2003, sera from 239 ocular patients, aged 3 to 80 years, were examined by ELISA and confirmed by Western blot test. Out of the 239 patients, 172 (72%) were seronegative and 67 (28%) were Toxocara seropositive; 95% CI (22-34%). The median age of Toxocara seropositive patients was 37.6 years. There was no significant difference in the number of Toxocara positive sera between the younger age group (≤14 years) and the older age group (>14 years), p>0.05. A high rate of Toxocara seropositivity in ocular patients should alert the ophthalmologists in Slovenia to include toxocariasis in the differential diagnosis of eye diseases more frequently.

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Original Article

A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea
Hyun-Young Park, Soo-Ung Lee, Sun Huh, Yoon Kong, Jean-Fran?ois Magnaval
Korean J Parasitol 2002;40(3):113-117.
Published online September 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.3.113

We investigated the sero-prevalence of toxocariasis among healthy Korean adults in 1999. A total of 314 sera from normal inhabitants in Whachon-gun, Gangwon-do, Korea was examined for specific antibody levels against excretory-secretory products of second stage larvae of Toxocara (TES). The presence of cross-reactions with other helminthiases such as cysticercosis, paragonimiasis, sparganosis or clonorchiasis was also checked by specific IgG ELISA. Sera showing positive reaction against TES were also tested by IgG immunoblot and by IgE ELISA. Out of 314 subjects, 16 was found to be positive by TES IgG ELISA and immunoblot, among whom 12 were also positive by TES IgE ELISA. Among the 16 seropositive samples, two sera showed positive reaction against Paragonimus and sparganum antigen, respectively. These results inferred that cross-reactions were negligible between toxocariasis and other helminthiases. Toxocariasis seroprevalence among Korean rural adults was detected to be approximately 5%.

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Mini Review

Highlights of human toxocariasis
Jean-Fran?ois Magnaval, Lawrence T. Glickman, Philippe Dorchies, Bruno Morassin
Korean J Parasitol 2001;39(1):1-11.
Published online March 31, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.1.1

Human toxocariasis is a helminthozoonosis due to the migration of Toxocara species larvae through human organism. Humans become infected by ingesting either embryonated eggs from soil (geophagia, pica), dirty hands or raw vegetables, or larvae from undercooked giblets. The diagnosis relies upon sensitive immunological methods (ELISA or western-blot) which use Toxocara excretory-secretory antigens. Seroprevalence is high in developed countries, especially in rural areas, and also in some tropical islands. The clinical spectrum of the disease comprises four syndromes, namely visceral larva migrans, ocular larva migrans, and the more recently recognized "common" (in adults) and "covert" (in children) pictures. Therapy of ocular toxocariasis is primarily based upon corticosteroids use, when visceral larva migrans and few cases of common or covert toxocariasis can be treated by anthelmintics whose the most efficient appeared to be diethylcarbamazine. When diagnosed, all of these syndromes require thorough prevention of recontamination (especially by deworming pets) and sanitary education.

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Case Report
Five cases of ocular toxocariasis confirmed by serology
Sung-Pyo Park, Inwon Park, Hyun-Young Park, Soo-Ung Lee, Sun Huh, Jean-Fran?ois Magnaval
Korean J Parasitol 2000;38(4):267-273.
Published online December 31, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.4.267

We report 5 cases of ocular toxocariasis in Korean adults complaining of visual impairment along with floating or bubbling sensation. Fundoscopic examination revealed a retinal detachment along with exudate in 4 cases. They all showed typical reaction by ELISA and immunoblot against Toxocra excretory-secretory antigen. One case showed high level of anti-Toxocara IgE antibodies (34,000 Toxocara units/L) as well as increased level of serum total IgE antibodies and the specific IgE antibodies for 3 inhalant antigens, suggesting that high level of anti-Toxocara IgE antibodies was associated with an atopic status. Clinical manifestations were improved after the sequential use of steroids then mebendazole. We also suggest that ocular toxocariasis should be thoroughly investigated even when an evocative uniocular inflammatory lesion is encountered in peripheral retina without a systematic disease.

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