Parasites, Hosts and Diseases

"UAE"

Original Articles

Susceptibility of Laboratory Rodents to Trichinella papuae: Lakkhana Sadaow, Pewpan M. Intapan, Thidarut Boonmars, Nimit Morakote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):629-632.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.629

Abstract
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Members of the genus Trichinella are small nematodes that can infect a wide range of animal hosts. However, their infectivity varies depending on the parasite and host species combination. In this study, we examined the susceptibility of 4 species of laboratory rodents, i.e., mice, rats, hamsters, and gerbils to Trichinella papuae, an emerging non-encapsulated Trichinella species. Trichinella spiralis and Trichinella pseudospiralis were also included in this study for comparison. Fifteen animals of each rodent species were infected orally with 100 muscle larvae of each Trichinella species. Intestinal worm burden was determined at day 6 and 10 post-inoculation (PI). The numbers of muscle larvae were examined at day 45 PI. The reproductive capacity index (RCI) of the 3 Trichinella species in different rodent hosts was determined. By day 6 PI, 33.2-69.6% of the inoculated larvae of the 3 Trichinella species became adult worms in the small intestines of the host animals. However, in rats, more than 96% of adult worms of all 3 Trichinella species were expelled from the gut by day 10 PI. In gerbils, only 4.8-18.1% of adult worms were expelled by day 10 PI. In accordance with the intestinal worm burden and the persistence of adults, the RCI was the highest in gerbils with values of 241.5±41.0 for T. papuae, 432.6±48 for T. pseudospiralis, and 528.6±20.6 for T. spiralis. Hamsters ranked second and mice ranked third in susceptibility in terms of the RCI, Rats yielded the lowest parasite RCI for all 3 Trichinella species. Gerbils may be an alternative laboratory animal for isolation and maintenance of Trichinella spp.

Citations

Citations to this article as recorded by

Viability of Trichinella spiralis in traditional sour pork fermentation and its inactivation by microwave heating: Implications for zoonotic risk and food safety
Atchara Artchayasawat, Benjamabhorn Pumhirunroj, Sukhonthip Khueangchiangkhwang, Thidarut Boonmars, Parichart Boueroy, Porntip Laummaunwai, Panaratana Rattanasuwan
Veterinary World.2025; : 1660. CrossRef
Real-time PCR versus traditional and Nano-based ELISA in early detection of murine trichinellosis
S. M. Mohammad, L. A. Hegazy, R. S. Abdel Hady, M. A. Salama, S. K. Hammad, S. M. Ibrahim
Journal of Helminthology.2023;[Epub] CrossRef
A potential herbal therapeutic for trichinellosis
Asmaa M. El-kady, Iman A. M. Abdel-Rahman, Eman Sayed, Majed H. Wakid, Hussah M. Alobaid, Khalil Mohamed, Eman Abdullah Alshehri, Hayam Elshazly, Wafa Abdullah I. Al-Megrin, Furhan Iqbal, Hatem A. Elshabrawy, Ashraf G. Timsah
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Reprint of: Survival of Trichinella spiralis in cured meat products
Annette Johne, Matthias Filter, Jennifer Gayda, Anja Buschulte, Niels Bandick, Karsten Nöckler, Anne Mayer-Scholl
Veterinary Parasitology.2021; 297: 109544. CrossRef
Human Outbreak of Trichinellosis Caused by Trichinella papuae Nematodes, Central Kampong Thom Province, Cambodia
Yannick Caron, Sotharith Bory, Michel Pluot, Mary Nheb, Sarin Chan, Sang Houn Prum, Sun Bun Hong Lim, Mala Sim, Yi Sengdoeurn, Ly Sovann, Virak Khieu, Isabelle Vallée, Hélène Yera
Emerging Infectious Diseases.2020; 26(8): 1759. CrossRef
Survival of Trichinella spiralis in cured meat products
Annette Johne, Matthias Filter, Jennifer Gayda, Anja Buschulte, Niels Bandick, Karsten Nöckler, Anne Mayer-Scholl
Veterinary Parasitology.2020; 287: 109260. CrossRef
Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera
Chalermchai Somboonpatarakun, Rutchanee Rodpai, Pewpan M. Intapan, Oranuch Sanpool, Lakkhana Sadaow, Chaisiri Wongkham, Tonkla Insawang, Thidarut Boonmars, Wanchai Maleewong
Parasitology Research.2018; 117(1): 201. CrossRef
Differential immune responses in mice infected with the tissue-dwelling nematode Trichinella zimbabwensis
W.N. Onkoba, M.J. Chimbari, J.M. Kamau, S. Mukaratirwa
Journal of Helminthology.2016; 90(5): 547. CrossRef

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Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR: Doaa M. Sultan, Marwa M. Khalil, Ahmed S. Abdouh, Wafaa F. Doleh, Abdul Aziz M. Al Muthanna; Korean J Parasitol 2009;47(3):227-233.
Published online August 28, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.3.227

Abstract
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Local malaria transmission in the United Arab Emirates (UAE) came to an end in 1997. Nevertheless, UAE has been subjected to substantial importation of malaria cases from abroad, concerning both UAE nationals and immigrants from malarious countries with a total number of 2,119 cases in 2007. To evaluate a new DNA extraction technique using nested PCR, blood samples were collected from 132 individuals who presented to Infectious Diseases Department in Rashid Hospital, Dubai, and Central Department of Malaria Control with fever and persistent headache. Giemsa-stained blood films and ELISA test for malaria antibodies were carried out for detection of Plasmodium infection. Plasmodium infections were identified with the genus-specific primer set and species differentiation using nested PCR. A rapid procedure for diagnosis of malaria infections directly from dried blood spots using for the first time DNA extract from FTA Elute cards was evaluated in contrast to extraction techniques using FTA classic cards and rapid boiling technique. Our new simple technique for DNA extraction using FTA Elute cards was very sensitive giving a sensitivity of 100% compared to 94% using FTA classic cards and 62% in the rapid boiling technique. No complex preparation of blood samples was required prior to the amplification. The production cost of DNA isolation in our PCR assay was much less in comparable to that of other DNA extraction protocols. The nested PCR detected plasmodial infection and could differentiate P. falciparum from P. vivax, and also detected the mixed infection.

Citations

Citations to this article as recorded by

Nucleic acid purification from dried blood spot on FTA Elute Card provides template for polymerase chain reaction for highly sensitive Plasmodium detection
Muneaki Hashimoto, Mika Bando, Jun-ichi Kido, Kazumichi Yokota, Toshihiro Mita, Kazuaki Kajimoto, Masatoshi Kataoka
Parasitology International.2019; 73: 101941. CrossRef
Genetic diversity and transmissibility of imported Plasmodium vivax in Qatar and three countries of origin
Mohammed H. Abdelraheem, Devendra Bansal, Mohammed A. Idris, Moawia M. Mukhtar, Muzamil M. Abdel Hamid, Zainb S. Imam, Sisay Getachew, Rakesh Sehgal, Hargobinder Kaur, Amal H. Gadalla, Salam Al-Hamidhi, Zainab Al-Hashami, Ali Al-Jabri, Ali A. Sultan, Hamz
Scientific Reports.2018;[Epub] CrossRef
A simple, efficient and inexpensive method for malaria parasites’ DNA catching from fixed Giemsa-stained blood slides
Abbas Ali Eskandarian, Sara Moradi, Saeed Abedi
Journal of Parasitic Diseases.2016; 40(3): 833. CrossRef
Development of new malaria diagnostics: matching performance and need
David Bell, Alessandra E. Fleurent, Michael C. Hegg, John D. Boomgard, Caitlin C. McConnico
Malaria Journal.2016;[Epub] CrossRef
Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination
E. C. Oriero, J. Jacobs, J.-P. Van Geertruyden, D. Nwakanma, U. D'Alessandro
Journal of Antimicrobial Chemotherapy.2015; 70(1): 2. CrossRef
Genetic diversity of Plasmodium falciparum and distribution of drug resistance haplotypes in Yemen
Salama Al-Hamidhi, Mohammed AK Mahdy, Zainab Al-Hashami, Hissa Al-Farsi, Abdulsalam M Al-mekhlafi, Mohamed A Idris, Albano Beja-Pereira, Hamza A Babiker
Malaria Journal.2013;[Epub] CrossRef
Quantitative detection of <i>Streptococcus mutans</i> from saliva using FTA<sup>TM</sup> elute cards and real-time polymerase chain reaction
Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren
American Journal of Molecular Biology.2013; 03(03): 148. CrossRef
An optimized method for elution of enteroviral RNA from a cellulose-based substrate
Yan Li, Hiromu Yoshida, Lu Wang, Zexin Tao, Haiyan Wang, Xiaojuan Lin, Aiqiang Xu
Journal of Virological Methods.2012; 186(1-2): 62. CrossRef
Comparative Analytical Utility of DNA Derived from Alternative Human Specimens for Molecular Autopsy and Diagnostics
Tara L. Klassen, Eva-Lotta von Rüden, Janice Drabek, Jeffrey L. Noebels, Alica M. Goldman
The Journal of Molecular Diagnostics.2012; 14(5): 451. CrossRef
Source of drug resistant Plasmodium falciparum in a potential malaria elimination site in Saudi Arabia
Hissa M. Al-Farsi, Zainab S. Al-Hashami, Saad M. Bin Dajem, Adel Ali H. Al-Sheikh, Ahmed Al-Qahtani, Albano Beja-Pereira, Mohamed A. Idris, Hamza A. Babiker
Infection, Genetics and Evolution.2012; 12(6): 1253. CrossRef