Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcusgranulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.
Citations
Citations to this article as recorded by
Progresses and challenges in
Strongyloides
spp. proteomics
Natalia Tiberti, Marcello Manfredi, Chiara Piubelli, Dora Buonfrate Philosophical Transactions of the Royal Society B: Biological Sciences.2024;[Epub] CrossRef
A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease Jing Xiao, Jian-Wen Wu, Yun-Zhuo Xin, Dong Song, Xiao-ping Gao, Mei Yin, Wei Zhao, Fu-lin Liu, Hao Wang, Jie Wang, Jia-Qing Zhao Parasitology Research.2024;[Epub] CrossRef
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response María Celeste Nicolao, Christian Rodriguez Rodrigues, Magalí B. Coccimiglio, Camila Ledo, Guillermo H. Docena, Andrea C. Cumino Parasites & Vectors.2023;[Epub] CrossRef
Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice With Echinococcus granulosus at Different Infection Stages and Their Immunomodulatory Functions Chunli Shi, Xiaojing Zhou, Wenjuan Yang, Jianwen Wu, Min Bai, Ying Zhang, Wei Zhao, Hui Yang, Atsushi Nagai, Mei Yin, Xiaoping Gao, Shuqin Ding, Jiaqing Zhao Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing Jing Xiao, Yazhou Zhu, Jianwen Wu, Min Bai, Yunzhuo Xin, Qiang Wang, Jiaqing Zhao Parasitology Research.2022; 121(7): 1993. CrossRef
Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts Andrea Maglioco, Facundo A. Agüero, María Pía Valacco, Alejandra Juárez Valdez, Margot Paulino, Alicia G. Fuchs Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition Hynek Mazanec, Peter Koník, Zdenko Gardian, Roman Kuchta International Journal for Parasitology.2021; 51(5): 327. CrossRef
Identification of Different Extracellular Vesicles in the Hydatid Fluid of Echinococcus granulosus and Immunomodulatory Effects of 110 K EVs on Sheep PBMCs Jing Yang, Jin'en Wu, Yong Fu, Lujun Yan, Yating Li, Xiaola Guo, Yong'e Zhang, Xiaoqiang Wang, Yujuan Shen, William C. Cho, Yadong Zheng Frontiers in Immunology.2021;[Epub] CrossRef
Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia Jie Wang, Jun He, Yucheng Fan, Fangjing Xu, Qian Liu, Ruhua He, Ru Yan Experimental and Therapeutic Medicine.2021;[Epub] CrossRef
Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages Li-Qun Wang, Ting-Li Liu, Pan-Hong Liang, Shao-Hua Zhang, Tao-Shan Li, Yan-Ping Li, Guang-Xue Liu, Li Mao, Xue-Nong Luo Parasites & Vectors.2020;[Epub] CrossRef
Hymenolepis nana and Hymenolepis diminuta are globally widespread zoonotic cestodes. Rodents are the main reservoir host of these cestodes. Brown rats (Rattus norvegicus) are the best known and most common rats, and usually live wherever humans live, especially in less than desirable hygiene conditions. Due to the little information of the 2 hymenolepidid species in brown rats in China, the aim of this study was to understand the prevalence and genetic characterization of H. nana and H. diminuta in brown rats in Heilongjiang Province, China. Total 114 fecal samples were collected from brown rats in Heilongjiang Province. All the samples were subjected to morphological examinations by microscopy and genetic analysis by PCR amplification of the mitochondrial cytochrome c oxidase subunit 1 (COX1) gene and the internal transcribed spacer 2 (ITS2) region of the nuclear ribosomal RNA gene. In total, 6.1% (7/114) and 14.9% (17/114) of samples were positive for H. nana and H. diminuta, respectively. Among them, 7 and 3 H. nana isolates were successfully amplified and sequenced at the COX1 and ITS2 loci, respectively. No nucleotide variations were found among H. nana isolates at either of the 2 loci. Seventeen H. diminuta isolates produced 2 different COX1 sequences while 7 ITS2 sequences obtained were identical to each other. The present results of H. nana and H. diminuta infections in brown rats implied the risk of zoonotic transmission of hymenolepiasis in China. These molecular data will be helpful to deeply study intra-specific variations within Hymenolepis cestodes in the future.
Citations
Citations to this article as recorded by
The Role of the Norway Rat, Rattus norvegicus, as a Reservoir of Zoonotic Helminth Species in the City of Barcelona (Spain) Màrius Vicent Fuentes, Pablo Puchades-Colera, Carla Gosálvez, Sandra Sáez-Durán, Maria Cholvi-Simó, Santiago Ruvira, Joan Sanxis-Furió, Jordi Pascual, Rubén Bueno-Marí, Sandra Franco, Víctor Peracho, Tomás Montalvo, María Trelis, Ángela L. Debenedetti, Ma Animals.2025; 15(3): 298. CrossRef
Gastrointestinal helminth infections and ectoparasitism in wild rodents along wildlife-human interfaces in Tanzania Venance T. Msoffe, Claus A. Thomas, Alfan A. Rija, Jahashi Nzalawahe, Abdul S. Katakweba, Gerald Misinzo, Ladslaus L. Mnyone International Journal for Parasitology: Parasites and Wildlife.2025; 26: 101040. CrossRef
Quercetin-loaded solid lipid nanoparticles for enhanced anti-helminthic activity Sunidhi Sharma, Ruchika Thukral, Lachhman Das Singla, Neena Singla, Diptiman Choudhury International Journal of Pharmaceutics.2025; 672: 125308. CrossRef
Sewer-associated rodents in countries with lower human development, a time-bomb for zoonoses? Clara Muñoz-Hernández, Ana Huertas-López, Woraporn Sukhumavasi, Moisés Gonzálvez Research in Veterinary Science.2025; 188: 105614. CrossRef
Treating Hymenolepiasis with Pumpkin Seeds: Effect on Ileum Histology and Dwarf Tapeworm Morphology Majed H. Wakid, Anan O. Alhawiti, Alaa S. Banjar, Rowaid M. Qahwaji, Hattan S. Gattan, Maimonah S. Alghanmi, Haifaa A. Mahjoub, Mohammad F. Al-Refai Acta Parasitologica.2025;[Epub] CrossRef
Prevalence of intestinal parasites in laboratory rats and mice and effectiveness of aqueous extracts from carob pods, Moringa oleifera, and Artemisia alba on the zoonotic parasite Hymenolepis diminuta infection Reham G. A. Anter, Hoda A. Abd-Ellatieff, Hanaa Mohammed, Ahmed Bendari, Rehab E. Mohamed, Ashraf Fawzy Mosa Ahmed, Rasha Kamal Elsaid Fahmy, Marwa Alkaffas, Fatima M. Ibrahim, Doaa Naguib, Asmaa Aboelabbas Gouda Acta Parasitologica.2025;[Epub] CrossRef
Morphometric and molecular identification of Hymenolepis diminuta in domestic rats (Rattus rattus) from urban slums in Northeastern Bangladesh: Implications for zoonotic transmission Tilak Chandra Nath, Md. Rokibul Hasan Shanto, Md. Masudur Rahman Khondaker, Mahfuja Akter, Md. Shakil Mahmud Supto, Proloy Chakraborty Tusher, Tarek Siddiki, Heeil Lee IJID One Health.2025; 8: 100077. CrossRef
Rodent-Borne Parasites and Human Disease: A Growing Public Health Concern Alfonso J. Rodriguez-Morales, Awad A. Shehata, Rokshana Parvin, Shadia Tasnim, Phelipe Magalhães Duarte, Shereen Basiouni Animals.2025; 15(18): 2681. CrossRef
Identification and histopathological assessment of intestinal Hymenolepis nana in pet hamsters in Guiyang, China Hongyan Wang, Ke Zhang, Xuanyin Cui, Xiaomao Li, Jiayu Hong, Yi Cheng, Rong Mou Veterinary Parasitology: Regional Studies and Reports.2025; 65: 101339. CrossRef
Hymenolepidid cestodes: Diversity, morphological and molecular characterization of a new species, and phylogeny of parasitic species of rodents from North and South America Natalia Beatriz Guerreiro Martins, Maria del Rosario Robles, Graciela Teresa Navone, Callejón Rocío Acta Tropica.2022; 231: 106480. CrossRef
Comparative comprehensive analysis on natural infections of Hymenolepis diminuta and Hymenolepis nana in commensal rodents S. K. Brar, N. Singla, L. D. Singla Helminthologia.2021; 58(3): 248. CrossRef
The dwarf tapeworm Hymenolepis nana in pet rodents in Slovakia—epidemiological survey and genetic analysis Júlia Jarošová, Daniela Antolová, Viliam Šnábel, Dana Miklisová, Serena Cavallero Parasitology Research.2020; 119(2): 519. CrossRef
Prevalence of intestinal parasitic infections among the Bulgarian population over a three year period (2015 – 2017) R. Harizanov, I. Rainova, N. Tsvetkova, I. Kaftandjiev, R. Borisova, A. Ivanova, M. Videnova Helminthologia.2020; 57(1): 12. CrossRef
Morphological, Molecular, and Pathological Appraisal ofHymenolepis nana(Hymenolepididae) Infecting Laboratory Mice (Mus musculus) Ebtsam Al-Olayan, Maha Elamin, Eman Alshehri, Abeer Aloufi, Zainab Alanazi, Mina Almayouf, Lamia Bakr, Rewaida Abdel-Gaber Microscopy and Microanalysis.2020; 26(2): 348. CrossRef
Molecular genotypes analysis of Cryptosporidium and Hymenolepis in rats on Lombok Island, Indonesia Ersandhi Resnhaleksmana, Wayan Tunas Artama, Mahardika Agus Wijayanti, Fihiruddin Fihiruddin International Journal of One Health.2020; 6(2): 123. CrossRef
Novel Linezolid analogues with antiparasitic activity against Hymenolepis nana Eleazar Alcántar-Zavala, Esteban Hernández-Guevara, Adrián Ochoa-Terán, Julio Montes-Ávila, Edgar A. Estrada-Zavala, Alex J. Salazar-Medina, Efraín Alday, Alberto Cabrera, Gerardo Aguirre, Valentín Miranda-Soto, Carlos Velazquez, Sylvia P. Díaz-Camacho, J Bioorganic Chemistry.2020; 105: 104359. CrossRef
Epidemiological and clinical characteristic of Hymenolepis diminuta infection – review of current literature Patrycja Kapczuk, Dariusz Chlubek, Irena Baranowska-Bosiacka Pomeranian Journal of Life Sciences.2020; 66(1): 29. CrossRef
Epidemiological and clinical characteristic of Hymenolepis diminuta infection – review of current literature Patrycja Kapczuk, Dariusz Chlubek, Irena Baranowska-Bosiacka Pomeranian Journal of Life Sciences.2020; 66(2): 32. CrossRef
Rodent-borne and rodent-related diseases in Iran Vahid Kazemi-Moghaddam, Rouhullah Dehghani, Mostafa Hadei, Samaneh Dehqan, Mohammad Mehdi Sedaghat, Milad Latifi, Shamim Alavi-Moghaddam Comparative Clinical Pathology.2019; 28(4): 893. CrossRef
Genetic diversity of the potentially therapeutic tapeworm Hymenolepis diminuta (Cestoda: Cyclophyllidea) Lucie Řežábková, Jan Brabec, Milan Jirků, Marc Dellerba, Roman Kuchta, David Modrý, William Parker, Kateřina Jirků Pomajbíková Parasitology International.2019; 71: 121. CrossRef
Comparative Study morphological and Molecular for infected Rodent with two cestoda Hymenolepis nana & H. diminuta In Al-Diwaniyah City, South Of Iraq Habeeb waseel kadhum shubber, Murtadha Nabeel, Murtadha Al-Tameemi Journal of Physics: Conference Series.2019; 1294(6): 062058. CrossRef
Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the pathogenicity of T. gondii in broiler chickens were rarely reported. The objective of the present study was to compare the pathogenicity of 5 different T. gondii strains (RH, CN, JS, CAT2, and CAT3) from various host species origin in 10-day-old chickens. Each group of chickens was infected intraperitoneally with 5×108, 1×108, 1×107, and 1×106 tachyzoites of the 5 strains, respectively. The negative control group was mockly inoculated with PBS alone. After infection, clinical symptoms and rectal temperatures of all the chickens were checked daily. Dead chickens during acute phage of the infection were checked for T. gondii tachyzoites by microscope, while living cases were checked for T. gondii infection at day 53 post-inoculation (PI) by PCR method. Histopathological sections were used to observe the pathological changes in the dead chickens and the living animals at day 53 PI. No significant differences were found in survival periods, histopathological findings, and clinical symptoms among the chickens infected with the RH, CN, CAT2, and CAT3 strains. Histopathological findings and clinical symptoms of the JS (chicken origin) group were similar to the others. However, average survival times of infected chickens of the JS group inoculated with 5×108 and 1×108 tachyzoites were 30.0 and 188.4 hr, respectively, significantly shorter than those of the other 4 mammalian isolates. Chickens exposed to 108 of T. gondii tachyzoites and higher showed acute signs of toxoplasmosis, and the lesions were relatively more severe than those exposed to lower doses. The results indicated that the pathogenicity of JS strain was comparatively stronger to the chicken, and the pathogenicity was dose-dependent.
Citations
Citations to this article as recorded by
Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species Jiyuan Fan, Hao Sun, Jiawen Fang, Yafan Gao, Haojie Ding, Bin Zheng, Qingming Kong, Xunhui Zhuo, Shaohong Lu Parasites & Vectors.2024;[Epub] CrossRef
First identified Toxoplasma gondii Type I in market-sold ducks in Fujian province, China: a significant for public health Si-Ang Li, Li-Yuan Huang, Xu-Dong Guo, Wen-Yuan Miao, Ying-Sheng Lin, Dong-Hui Zhou Poultry Science.2024; 103(9): 104024. CrossRef
Epidemiological investigation and reinfection evaluation of Toxoplasma gondii in chickens in Shandong Province, China Xiao Chen, Pei Sun, Junpeng Chen, Qianqian Tan, Jinxuan Li, Xiaomei Liu, Qianqian Xiao, Hongmei Li, Xiaomin Zhao, Ningning Zhao, Xiao Zhang Experimental Parasitology.2022; 238: 108276. CrossRef
Experimental infection of Toxoplasma gondii in specific pathogen-free and commercial broiler chicks Xiao Chen, Junpeng Chen, Qianqian Tan, Jinxuan Li, Xiaomei Liu, Qianqian Xiao, Hongmei Li, Xiaomin Zhao, Xiao Zhang Comparative Immunology, Microbiology and Infectious Diseases.2022; 90-91: 101890. CrossRef
Application of Toxoplasma gondii-specific SAG1, GRA7 and BAG1 proteins in serodiagnosis of animal toxoplasmosis Tongsheng Qi, Jingkai Ai, Yali Sun, Hejia Ma, Ming Kang, Xiaoqian You, Jixu Li Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade J. P. Dubey, H. F. J. Pena, C. K. Cerqueira-Cézar, F. H. A. Murata, O. C. H. Kwok, Y. R. Yang, S. M. Gennari, C. Su Parasitology.2020; 147(12): 1263. CrossRef
Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact S. Stelzer, W. Basso, J. Benavides Silván, L.M. Ortega-Mora, P. Maksimov, J. Gethmann, F.J. Conraths, G. Schares Food and Waterborne Parasitology.2019; 15: e00037. CrossRef
Characterisation of susceptibility of chicken macrophages to infection with Toxoplasma gondii of type II and III strains Irene Malkwitz, Angela Berndt, Arwid Daugschies, Berit Bangoura Experimental Parasitology.2018; 187: 22. CrossRef
Chicken line-dependent mortality after experimental infection with three type IIxIII recombinant Toxoplasma gondii clones G. Schares, D.C. Herrmann, P. Maksimov, B. Matzkeit, F.J. Conraths, G. Moré, R. Preisinger, S. Weigend Experimental Parasitology.2017; 180: 101. CrossRef
Diagnosis of Toxoplasmosis Using Affinity Purified Fraction of Tachyzoites Local Isolate Soad E. Hassan, Nagwa I. Toaleb, Raafat M. Shaapan, Eman Hussien Abdel-Rahm, Ehab Kotb Elmahallaw Research Journal of Parasitology.2016; 11(1): 13. CrossRef
Seroprevalence of Toxoplasma gondii infection among patients with hand, foot and mouth disease in Henan, China: a hospital-based study Shuai Wang, Chunwei Lan, Luwen Zhang, Haizhu Zhang, Zhijun Yao, Dong Wang, Jingbo Ma, Jiarong Deng, Shiguo Liu Infectious Diseases of Poverty.2015;[Epub] CrossRef
First
Prev
