Our objective was to investigate whether inflammatory microenvironment induced by Trichomonas vaginalis infection can stimulate proliferation of prostate cancer (PCa) cells in vitro and in vivo mouse experiments. The production of CXCL1 and CCL2 increased when cells of the mouse PCa cells (TRAMP-C2 cell line) were infected with live T. vaginalis. T. vaginalis-conditioned medium (TCM) prepared from co-culture of PCa cells and T. vaginalis increased PCa cells migration, proliferation and invasion. The cytokine receptors (CXCR2, CCR2, gp130) were expressed higher on the PCa cells treated with TCM. Pretreatment of PCa cells with antibodies to these cytokine receptors significantly reduced the proliferation, mobility and invasiveness of PCa cells, indicating that TCM has its effect through cytokine-cytokine receptor signaling. In C57BL/6 mice, the prostates injected with T. vaginalis mixed PCa cells were larger than those injected with PCa cells alone after 4 weeks. Expression of epithelial-mesenchymal transition markers and cyclin D1 in the prostate tissue injected with T. vaginalis mixed PCa cells increased than those of PCa cells alone. Collectively, it was suggested that inflammatory reactions by T. vaginalis-stimulated PCa cells increase the proliferation and invasion of PCa cells through cytokine-cytokine receptor signaling pathways.
Citations
Citations to this article as recorded by
The role of proinflammatory cytokines and CXC chemokines (CXCL1–CXCL16) in the progression of prostate cancer: insights on their therapeutic management Amin Ullah, Wang Jiao, Bairong Shen Cellular & Molecular Biology Letters.2024;[Epub] CrossRef
Point-of-Care Diagnostic for Trichomonas vaginalis, the Most Prevalent, Non-Viral Sexually Transmitted Infection John F. Alderete, Hermes Chan Pathogens.2023; 12(1): 77. CrossRef
Involvement of Macrophages in Proliferation of Prostate Cancer Cells Infected with Trichomonas vaginalis Kyu-Shik Kim, Hong-Sang Moon, Sang-Su Kim, Jae-Sook Ryu The Korean Journal of Parasitology.2021; 59(6): 557. CrossRef
Trichomoniasis is the most common curable sexually-transmitted infection (STI) worldwide. There are few reports on the prevalence of Trichomonas vaginalis in Korea. The purpose of this study was to examine the prevalence of trichomoniasis by PCR in Guri city, Korea. All adult women who visited Hanyang University Guri Hospital for health screening within the National Health Care Service were invited to participate in the study, and 424 women were enrolled between March and June 2011. PCR was used to detect Trichomonas vaginalis using primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Fourteen women (3.3%) were found to have T. vaginalis. All were over 50, and they were significantly older on average than the 410 Trichomonas-negative women (mean ages 63.4 vs 55.3 years). It seems that T. vaginalis infection is not rare in women receiving health screening, especially among those over 50.
Citations
Citations to this article as recorded by
Molecular diagnosis of Trichomonas vaginalis in liquid-based Papanicolaou samples in Shiraz, southern Iran Mohammad Saleh Bahreini, Samaneh Sedghi, Yalda Badalzadeh, Mohammad Hossein Motazedian, Manouchehr Shirani, Sareh Sami Jahromi, Aref Teimouri, Mahmoud Agholi, Qasem Asgari BMC Women's Health.2023;[Epub] CrossRef
Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene Fakun Li, Yangyang Deng, Wanxin Sheng, Xihui Gao, Weijuan Wang, Zhili Chu, Xuefang Mei, Zhenke Yang, Xiaowei Tian, Shuai Wang, Zhenchao Zhang Journal of Eukaryotic Microbiology.2023;[Epub] CrossRef
Molecular characterization of Trichomonas infections in women of Ilam City, southwestern Iran Maryam Alikhani, Mohammad Akhoundi, Denis Sereno, Jahangir Abdi, Razi Naserifar, Mohammad Reza Mahmoudi, Asad Mirzaei Parasitology Research.2022; 121(6): 1631. CrossRef
Status of common parasitic diseases in Korea in 2019 Sun Huh Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
First
Prev
