Skip to main navigation Skip to main content
  • KSPTM
  • E-Submission

PHD : Parasites, Hosts and Diseases

OPEN ACCESS
ABOUT
BROWSE ARTICLES
FOR CONTRIBUTORS

Page Path

1
results for

"autofluorescence"

Article category

Keywords

Publication year

Authors

"autofluorescence"

Original Article

Application of Autofluorescence for Confocal Microscopy to Aid in Archaeoparasitological Analyses
Johnica Jo Morrow, Christian Elowsky
Korean J Parasitol 2019;57(6):581-585.
Published online December 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.6.581
Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.

Citations

Citations to this article as recorded by  Crossref logo
  • Label-free spectral confocal reflectance microscopy for ex vivo neuroimaging and neural structure visualization
    Reinher Pimentel-Domínguez, Rainald Pablo Ordaz, Abraham J. Cisneros-Mejorado, Rogelio O. Arellano, Remy Avila
    Methods.2025; 241: 140.     CrossRef
  • Fluorescence Microscopy with Deep UV, Near UV, and Visible Excitation forIn SituDetection of Microorganisms
    Noel Case, Nikki Johnston, Jay Nadeau
    Astrobiology.2024; 24(3): 300.     CrossRef
  • Analytical Techniques for the Preservation of Cultural Heritage: Frontiers in Knowledge and Application
    Mina Magdy
    Critical Reviews in Analytical Chemistry.2022; 52(6): 1171.     CrossRef
  • Paleomicrobiology of the human digestive tract: A review
    Riccardo Nodari, Michel Drancourt, Rémi Barbieri
    Microbial Pathogenesis.2021; 157: 104972.     CrossRef
  • Exploring confocal laser scanning microscopy (CLSM) and fluorescence staining as a tool for imaging and quantifying traces of marine microbioerosion and their trace‐making microendoliths
    Philipp‐Konrad Schätzle, Max Wisshak, Andreas Bick, André Freiwald, Alexander Kieneke
    Journal of Microscopy.2021; 284(2): 118.     CrossRef
  • 5,791 View
  • 107 Download
  • 5 Web of Science
  • Crossref