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Functional Genes and Proteins of Clonorchis sinensis: Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong; Korean J Parasitol 2009;47(Suppl):S59.
Published online October 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.S.S59

Abstract
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During the past several decades, researches on parasite genetics have progressed from biochemical and serodiagnostic studies to protein chemistry, molecular biology, and functional gene studies. Nowadays, bioinformatics, genomics, and proteomics approaches are being applied by Korean parasitology researchers. As for Clonorchis sinensis, investigations have been carried out to identify its functional genes using forward and reverse genetic approaches and to characterize the biochemical and biological properties of its gene products. The authors review the proteins of cloned genes, which include antigenic proteins, physiologic and metabolic enzymes, and the gene expression profile of Clonorchis sinensis.

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Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
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Identification, immunolocalization, and characterization analyses of an exopeptidase of papain superfamily, (cathepsin C) from Clonorchis sinensis
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Proteomic identification of potential Clonorchis sinensis excretory/secretory products capable of binding and activating human hepatic stellate cells
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Characterization of the secreted cathepsin B cysteine proteases family of the carcinogenic liver fluke Clonorchis sinensis
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ClonorESTdb: a comprehensive database for Clonorchis sinensis EST sequences
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Adult Opisthorchis felineus major protein fractions deduced from transcripts: Comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis
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Experimental Parasitology.2013; 135(2): 297. CrossRef
Biochemical characterization and functional analysis of fructose-1,6-bisphosphatase from Clonorchis sinensis
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Genetic diversity of nuclear ITS1–5.8S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East
Yulia V. Tatonova, Galina N. Chelomina, Vladimir V. Besprosvannykh
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Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2
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Developmental Transcriptomic Features of the Carcinogenic Liver Fluke, Clonorchis sinensis
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Original Articles

Effects of gamma-irradiation on the infectivity and chromosome aberration of Clonorchis sinensis: Gab-Man Park, Tai-Soon Yong; Korean J Parasitol 2003;41(1):41-45.
Published online March 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.1.41

Abstract
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Effects of gamma irradiation on the worm survival and chromosomal aberration of Clonorchis sinensis were studied. The metacercariae irradiated with various amounts of gamma radiation (ranging from 5 Gy to 50 Gy) were fed to rats, and the effects were compared with those of non-irradiated controls. Recovery rates of adult worms in irradiated groups were reduced gradually as increasing of the irradiation doses. No worm was recovered from rats which were fed with 50 Gy irradiated metacercariae. The chromosome number was 2n = 56 in all worms from all experimental groups. However, the groups irradiated with 20 Gy, 25 Gy or 30 Gy showed variations in the chromosome number, depending on different cells in the same individual. Radiation doses used in this study did not appear to induce chromosome aberrations, however, irradiation with 30 Gy showed slightly reduced chromosome size.

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Frits Franssen, C?dric Gerard, Anamaria Cozma-Petru?, Madalena Vieira-Pinto, Anet Re?ek Jambrak, Neil Rowan, Peter Paulsen, Miroslaw Rozycki, Kristoffer Tysnes, David Rodriguez-Lazaro, Lucy Robertson
Trends in Food Science & Technology.2018;[Epub] CrossRef
Effects of X-ray on the metacestodes of Echinococcus granulosus in vitro
Rui Mao, Ge Wu, Hui Wang, Pengfei Lu, Jun Li, Haitao Li, Aimudula Ainiwaer, Yiwei Bai, Mingyang Shu, Yongxing Bao, Wenbao Zhang
BMC Infectious Diseases.2017;[Epub] CrossRef
Immunization and challenge shown by hamsters infected withOpisthorchis viverrinifollowing exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke
A. Papatpremsiri, P. Junpue, A. Loukas, P.J. Brindley, J.M. Bethony, B. Sripa, T. Laha
Journal of Helminthology.2016; 90(1): 39. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Gene expression of Clonorchis sinensis metacercaria induced by gamma irradiation
Tae Im Kim, Pyo Yun Cho, Kwang Jin Song, Shunyu Li, Sung-Jong Hong, Suk Won Park, Jong-Yil Chai, Eun-Hee Shin
Parasitology Research.2008; 102(6): 1143. CrossRef

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Chromosomes of the liver fluke, Clonorchis sinensis: Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong; Korean J Parasitol 2000;38(3):201-206.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.201

Abstract
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A karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, Clonorchis sinensis (Trematoda: Opisthorchiidae), collected from Korea and China. Chromosome preparations were made by means of air-drying method. The chromosome number was 2n=56 in both Korean and Chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. However, the karyotypes showed some differences between Korean and Chinese flukes. The karyotype of liver flukes from Korea consisted of three metacentric pairs, one meta-/submetacentric pair, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes. On the other hand, liver flukes from China consisted of two metacentric pairs, two meta-/submetacentric pairs, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes.

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Maria Y. Pakharukova, Viatcheslav A. Mordvinov
Parasitology.2022; 149(10): 1306. CrossRef
High-quality reference genome for Clonorchis sinensis
Neil D. Young, Andreas J. Stroehlein, Liina Kinkar, Tao Wang, Woon-Mok Sohn, Bill C.H. Chang, Parwinder Kaur, David Weisz, Olga Dudchenko, Erez Lieberman Aiden, Pasi K. Korhonen, Robin B. Gasser
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Robin B. Gasser, Patrick Tan, Bin Tean Teh, Sopit Wongkham, Neil D. Young
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Cytogenetics of Aspidogaster limacoides (Trematoda, Aspidogastrea): karyotype, spermatocyte division, and genome size
Marta Bombarová, Marta Špakulová, Martin Kello, Petr Nguyen, Eva Bazsalovicsová, Ivica Králová-Hromadová
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Krystyna Cwiklinski, John Pius Dalton, Philippe J Dufresne, James La Course, Diana JL Williams, Jane Hodgkinson, Steve Paterson
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The Opisthorchis viverrini genome provides insights into life in the bile duct
Neil D. Young, Niranjan Nagarajan, Suling Joyce Lin, Pasi K. Korhonen, Aaron R. Jex, Ross S. Hall, Helena Safavi-Hemami, Worasak Kaewkong, Denis Bertrand, Song Gao, Qihui Seet, Sopit Wongkham, Bin Tean Teh, Chaisiri Wongkham, Pewpan Maleewong Intapan, Wan
Nature Communications.2014;[Epub] CrossRef
Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
Jiufeng Sun, Yan Huang, Huaiqiu Huang, Pei Liang, Xiaoyun Wang, Qiang Mao, Jingtao Men, Wenjun Chen, Chuanhuan Deng, Chenhui Zhou, Xiaoli Lv, Juanjuan Zhou, Fan Zhang, Ran Li, Yanli Tian, Huali Lei, Chi Liang, Xuchu Hu, Jin Xu, Xuerong Li, XinbingYu, Dav
PLoS ONE.2013; 8(6): e67006. CrossRef
Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives
Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu
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Clarification of the systematic position ofCercariaeum crassumWesenberg-Lund, 1934 (Digenea), based on karyological analysis and DNA sequences
R. Petkevičiūtė, V. Stunžėnas, G. Stanevičiūtė
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Comparative cytogenetics of opisthorchid species (Trematoda, Opisthorchiidae)
Kira S. Zadesenets, Alexei V. Katokhin, Viatcheslav A. Mordvinov, Nikolay B. Rubtsov
Parasitology International.2012; 61(1): 87. CrossRef
Functional Genes and Proteins of Clonorchis sinensis
Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong
The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Molecular genetic profiles among individual Clonorchis sinensis adults collected from cats in two geographic regions of China revealed by RAPD and MGE-PCR methods
De-Hua Lai, Qiao-Ping Wang, Wen Chen, Lian-Shun Cai, Zhong-Dao Wu, Xing-Quan Zhu, Zhao-Rong Lun
Acta Tropica.2008; 107(2): 213. CrossRef
Genetic comparison of liver flukes, Clonorchis sinensis and Opisthorchis viverrini, based on rDNA and mtDNA gene sequences
Gab-Man Park
Parasitology Research.2007; 100(2): 351. CrossRef
Gene discovery for the carcinogenic human liver fluke, Opisthorchis viverrini
Thewarach Laha, Porntip Pinlaor, Jason Mulvenna, Banchob Sripa, Manop Sripa, Michael J Smout, Robin B Gasser, Paul J Brindley, Alex Loukas
BMC Genomics.2007;[Epub] CrossRef
Clonorchiasis: an update
H.-J. Rim
Journal of Helminthology.2005; 79(3): 269. CrossRef
Effects of gamma-irradiation on the infectivity and chromosome aberration of Clonorchis sinensis
Gab-Man Park, Tai-Soon Yong
The Korean Journal of Parasitology.2003; 41(1): 41. CrossRef

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Karyotypes on three species of Chinese mesogastropod snails, Semisulcospira libertina, S. dolichostoma and Viviparus rivularis: Gab-Man Park, Jae-Jin Kim, Pyung-Rim Chung, Yong Wang, Duk-Young Min; Korean J Parasitol 1999;37(1):5-11.
Published online March 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.1.5

Abstract
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Three species of the families Viviparidae and Pleuroceridae, the first intermediate host of paragonimiasis, metagonimiasis and echinostomiasis were studied cytologically. The observed diploid chromosome number was as follows: Semisulcospira libertina 36, S. dolichostoma 34, and Viviparus rivularis 64. The mitotic chromosome complement of S. libertina has nine metacentric pairs and nine submetacentric pairs, and S. dolichostoma has three metacentric pairs and 14 submetacentric pairs of chromosomes. Viviparus rivularis showed two metacentric pairs and 30 submetacentric pairs of chromosomes.

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An adaptable chromosome preparation methodology for use in invertebrate research organisms
Longhua Guo, Alice Accorsi, Shuonan He, Carlos Guerrero-Hernández, Shamilene Sivagnanam, Sean McKinney, Matthew Gibson, Alejandro Sánchez Alvarado
BMC Biology.2018;[Epub] CrossRef
Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes
Jae-Suk Choi, Joo-Wan Kim, Jeong Been Park, Sang Eun Pyo, Yong-Ki Hong, Sae Kwang Ku, Mi-Ryung Kim
International Journal of Molecular Medicine.2017; 39(6): 1437. CrossRef
Karyotype of Arion vulgaris Moquin-Tandon, 1856 (Gastropoda: Pulmonata: Arionidae)
Alexandr V. Garbar, Natalia S. Kadlubovska
Folia Malacologica.2014;[Epub] CrossRef

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Brief Communication

Karyotype analysis of Neodiplostomum seoulense: Gab-Man Park, Soo-Ung Lee, Hyun-Young Park, Sun Huh; Korean J Parasitol 1998;36(4):277-279.
Published online December 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.4.277

Abstract
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A karyotype analysis of the chromosome of Neodiplostomum seoulense, one of causative agents of human intestinal trematodiasis, was done from the gonad tissue by the squashing method. The chromosome number was n = 10 and 2n = 20. Chromosome length was 1.30-4.0 ?m. Chromosome pairs in the complement consisted of two pairs of metacentric, five pairs of submetacentrics/subtelocentric and three pairs of telocentric chromosomes. These data were comparable with those of other intestinal trematodes.

Citations

Citations to this article as recorded by

Occurrence of a Diploid Type and a New First Intermediate Host of a Human Lung Fluke, Paragonimus westermani, in Korea
Gab Man Park, K.J. Lee, K.I. Im, H. Park, T.S. Yong
Experimental Parasitology.2001; 99(4): 206. CrossRef
Chromosomes of the liver fluke, Clonorchis sinensis
Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong
The Korean Journal of Parasitology.2000; 38(3): 201. CrossRef
A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea
Soo-Ung Lee, Sun Huh, Gab-Man Park, Jong-Yil Chai
The Korean Journal of Parasitology.1999; 37(4): 237. CrossRef