Parasites, Hosts and Diseases

"collagen"

Original Articles

Ameliorative Effect of Bone Marrow-Derived Stem Cells on Injured Liver of Mice Infected with Schistosoma mansoni: Magda M. El-Mahdi, Wafaa A. Mansour, Olfat Hammam, Noha A. Mehana, Taghreed M. Hussein; Korean J Parasitol 2014;52(2):151-162.
Published online April 18, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.2.151

Abstract
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The technique of stem cells or hepatocytes transplantation has recently improved in order to bridge the time before whole-organ liver transplantation. In the present study, unfractionated bone marrow stem cells (BMSCs) were harvested from the tibial and femoral marrow compartments of male mice, which were cultured in Dulbecco's modified Eagle's medium (DMEM) with and without hepatocyte growth factor (HGF), and then transplanted into Schistosoma mansoni-infected female mice on their 8th week post-infection. Mice were sacrificed monthly until the third month of bone marrow transplantation, serum was collected, and albumin concentration, ALT, AST, and alkaline phosphatase (ALP) activities were assayed. On the other hand, immunohistopathological and immunohistochemical changes of granuloma size and number, collagen content, and cells expressing OV-6 were detected for identification of liver fibrosis. BMSCs were shown to differentiate into hepatocyte-like cells. Serum ALT, AST, and ALP were markedly reduced in the group of mice treated with BMSCs than in the untreated control group. Also, granuloma showed a marked decrease in size and number as compared to the BMSCs untreated group. Collagen content showed marked decrease after the third month of treatment with BMSCs. On the other hand, the expression of OV-6 increased detecting the presence of newly formed hepatocytes after BMSCs treatment. BMSCs with or without HGF infusion significantly enhanced hepatic regeneration in S. mansoni-induced fibrotic liver model and have pathologic and immunohistopathologic therapeutic effects. Also, this new therapeutic trend could generate new hepatocytes to improve the overall liver functions.

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Anti-fibrotic Effect of Oral Versus Intraperitoneal Administration of Gold Nanoparticles in Hepatic Schistosoma mansoni-Infected Mice
Shahira Abdelaziz Ali Ahmed, Samer Eid Mohamed Gad, Omima Mohamed Eida, Laila Mohamed Makhlouf
Acta Parasitologica.2024; 69(1): 190. CrossRef
The Role of Praziquantel in the Prevention and Treatment of Fibrosis Associated with Schistosomiasis: A Review
Xuehua Niu, Tao Hu, Ye Hong, Xiaoyan Li, Yuzhou Shen, Wei Wang
Journal of Tropical Medicine.2022; 2022: 1. CrossRef
Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni-infection
Muslimah Alsulami, Rewaida Abdel-Gaber
Acta Parasitologica.2021; 66(1): 136. CrossRef
hUCMSC-extracellular vesicles downregulated hepatic stellate cell activation and reduced liver injury in S. japonicum-infected mice
Liyang Dong, Yanan Pu, Xiaojun Chen, Xin Qi, Lina Zhang, Lei Xu, Wei Li, Yongbin Ma, Sha Zhou, Jifeng Zhu, Yalin Li, Xuefeng Wang, Chuan Su
Stem Cell Research & Therapy.2020;[Epub] CrossRef
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Therapeutic Potential of Mesenchymal Stem Cells on Early and Late Experimental Hepatic Schistosomiasis Model
Shahinaz F. El-Shennawy, Heba E. Abdel Aaty, Nehal A. Radwan, Dina M. Abdel-Hameed, Yosra H. Alam-Eldin, Ayman M. El-Ashkar, Fatma A. Abu-Zahra
Journal of Parasitology.2015; 101(5): 587. CrossRef

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Molecular Cloning and Characterization of a Paramyosin from Clonorchis sinensis: Tae-Joon Park, Jung-Mi Kang, Byoung-Kuk Na, Woon-Mok Sohn; Korean J Parasitol 2009;47(4):359-367.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.359

Abstract
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Paramyosin is a myofibrillar protein present in helminth parasites and plays multifunctional roles in host-parasite interactions. In this study, we identified the gene encoding paramyosin of Clonorchis sinensis (CsPmy) and characterized biochemical and immunological properties of its recombinant protein. CsPmy showed a high level of sequence identity with paramyosin from other helminth parasites. Recombinant CsPmy (rCsPmy) expressed in bacteria had an approximate molecular weight of 100 kDa and bound both human collagen and complement 9. The protein was constitutively expressed in various developmental stages of the parasite. Imunofluorescence analysis revealed that CsPmy was mainly localized in the tegument, subtegumental muscles, and the muscle layer surrounding the intestine of the parasite. The rCsPmy showed high levels of positive reactions (74.6%, 56/75) against sera from patients with clonorchiasis. Immunization of experimental rats with rCsPmy evoked high levels of IgG production. These results collectively suggest that CsPmy is a multifunctional protein that not only contributes to the muscle layer structure but also to non-muscular functions in host-parasite interactions. Successful induction of host IgG production also suggests that CsPmy can be applied as a diagnostic antigen and/or vaccine candidate for clonorchiasis.

Citations

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