Chikungunya virus (CHIKV), a tropical pathogen, has re-emerged and has massive outbreaks abruptly all over the world. Containing many dominant epitopes, the envelope E2 protein of CHIKV has been explored for the vaccination or diagnosis. In the present study, the antigenicity of a recombinant expressed intrinsically disorder domain (IUD) of E2 was tested for the detection of the antibody against CHIKV through western blot method. The gene of the IUD of E2 was inserted into 2 different vectors and expressed as recombinant GST-E2 and recombinant MBP-E2 fusion protein, respectively. Two kinds of fusion proteins were tested with 30 CHIKV patient sera and 30 normal sera, respectively. Both proteins were detected by 25 patients sera (83.3%) and 1 normal serum (3.3%). This test showed a relatively high sensitivity and very high specificity of the recombinant E2 proteins to be used as diagnostic antigens against CHIKV infection.
Citations
Citations to this article as recorded by
Medical viruses: diagnostic techniques Harsh, Pratima Tripathi Virology Journal.2023;[Epub] CrossRef
Diagnostic accuracy of serological tests for the diagnosis of Chikungunya virus infection: A systematic review and meta-analysis Anna Andrew, Tholasi Nadhan Navien, Tzi Shien Yeoh, Marimuthu Citartan, Ernest Mangantig, Magdline S. H. Sum, Ewe Seng Ch’ng, Thean-Hock Tang, Guilherme S. Ribeiro PLOS Neglected Tropical Diseases.2022; 16(2): e0010152. CrossRef
An Overview of Laboratory Diagnosis of Central Nervous System Viral Infections Sharifah Aliah Diyanah Syed Hussin, Ang-Lim Chua, Hassanain Al-Talib, Shamala Devi Sekaran, Seok Mui Wang Journal of Pure and Applied Microbiology.2022; 16(4): 2225. CrossRef
Molecular and Immunological Diagnostic Techniques of Medical Viruses Daniel Hussien Reta, Tesfaye Sisay Tessema, Addis Simachew Ashenef, Adey Feleke Desta, Wajana Lako Labisso, Solomon Tebeje Gizaw, Solomon Mequanente Abay, Daniel Seifu Melka, Fisseha Alemu Reta International Journal of Microbiology.2020; 2020: 1. CrossRef
Apigenin enhances the cisplatin cytotoxic effect through p53-modulated apoptosis Rui Liu, Ping Ji, Bin Liu, Haishi Qiao, Xia Wang, Likun Zhou, Ting Deng, Yi Ba Oncology Letters.2017; 13(2): 1024. CrossRef
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