Parasites, Hosts and Diseases

"enzyme-linked immunosorbent assay"

Brief Communication

Enzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea: Sung-Hwan Wee, Chung-Gil Lee, Hoo-Don Joo, Yung-Bai Kang; Korean J Parasitol 2001;39(3):261-264.
Published online September 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.3.261

Abstract
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Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspected while one was positive by ELISA. But western blot analyses employed for further confirmation have shown that all of 10 samples did not react to larval excretory-secretory product antigens. These results indicate that pig breeding farms included in the present study are free from trichinellosis. However, it does not mean Korea is free from trichinellosis since human trichinellosis has recently been reported. The necessity of continued surveillance for trichinellosis in both pigs and wild animals was discussed.

Citations

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Veterinární medicína.2015; 60(4): 181. CrossRef
Primary characterization and assessment of a T. spiralis antigen for the detection of Trichinella infection in pigs
Aleksandar Zocevic, Sandrine A. Lacour, Pauline Mace, Baldissera Giovani, Aurelie Grasset-Chevillot, Isabelle Vallee, Pascal Boireau
Veterinary Parasitology.2014; 205(3-4): 558. CrossRef
Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)
Leigh Cuttell, Maria Angeles Gómez-Morales, Beth Cookson, Peter J. Adams, Simon A. Reid, Paul B. Vanderlinde, Louise A. Jackson, C. Gray, Rebecca J. Traub
Veterinary Parasitology.2014; 199(3-4): 179. CrossRef
Seroprevalence of trichinellosis in domestic animals in northwestern Vietnam
N. Vu Thi, N.V. De, N. Praet, L. Claes, S. Gabriël, P. Dorny
Veterinary Parasitology.2013; 193(1-3): 200. CrossRef
Development and evaluation of an immunochromatographic strip for trichinellosis detection
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Veterinary Parasitology.2006; 137(3-4): 286. CrossRef

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Original Articles

Experimental infection of Anopheles sinensis with Korean isolates of Plasmodium vivax: Hyeong Woo Lee, Shin Hyeong Cho, E-Hyun Shin, Jong Soo Lee, Joon-Sang Lee, Jong-Yil Chai, Soon-Hyung Lee, Tong Soo Kim; Korean J Parasitol 2001;39(2):177-183.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.177

Abstract
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The
objective
s of the present study were to (1) determine the susceptibility of Anopheles sinensis to Korean isolates of Plasmodium vivax, (2) establish a method to collect large quantities of P. vivax sporozoites for use as antigen in seroepidemiological studies, and (3) investigate the characteristics of Korean isolates of P. vivax sporozoites. Females of Anopheles sinensis were collected at non-epidemic area, Seokwha-ri, Cheongwon-gun and Chungcheongbuk-do using tent-trap methods coupled with dry ice. The females were artificially infected with gametocytes of P. vivax using blood obtained from P. vivax malaria patients. Individual mosquitoes were infected using either a parafilm-covered glass feeding apparatus or were allowed to feed on naturally infected volunteers. Mosquitoes were sacrificed between 16 and 18 days post-feeding and an enzyme-linked immunosorbent assay (ELISA) was used to detect sporozoites. Four (33.4%) of 12 mosquitoes, which were fed on naturally infected volunteers directly, were positive for sporozoites. In cases, the mosquitoes allowed to feed on whole blood which were extract from three different patients with heparin treated vacuutainers using a parafilm-covered glass apparatus. Two of 55 (3.6%) were positive which blood sample was maintained at room temperature for 8 hours, 1 of 68 (1.5%) was positive which blood was maintained at 4℃ for 24 hours and 1 of 47 (2.3%) was positive at 4℃ for 48 hours. The mean number of sporozoites was estimated about 818 (n=8; range of 648-1,056) based on optical density values of ELISA.

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Variation of antigenicity and serological reaction to Pneumocystis carinii in Korea: Hyun-Young Park, Soo-Ung Lee, Seoung-Wan Chae, Sun Huh, Jae-Ran Yu, Jin Kim, Sung-Tae Hong; Korean J Parasitol 1999;37(2):109-115.
Published online June 30, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.2.109

Abstract
PDF

The present study observed the variation of antigenicity of Pneumocystis carinii and serum IgG antibody reaction to the antigens from different localities in Korea. Antigens of rat P. carinii and sera of inhabitants were collected at Chunchon, Chungju, Kwangju, and Seoul during 1995-1996. Enzyme-Linked Immunosorbent Assay and immunoblot were used for immune reaction. Absorbance of 1,294 human sera ranged between 0.01 and 0.93. Sera from Chunchon showed higher absorbances than those from other areas. Immunoblotting revealed IgG antibody reactions to 116, 100, and 45-55 kDa antigenic bands of rat P. carinii, but the frequencies of positive reaction to individual bands were variable by localities. Total 62.6% of the sera showed the reaction to 116 kDa band while 37.7% reacted to 100 kDa band and 32.0% to 45-55 kDa bands. For the reaction to 116 kDa, the reaction rate was 60.0% to 82.6% by localities. It is found that the reaction rates of the human sera to rat P. carinii antigen are variable according to the localities. Also, the high molecular antigen of 116 kDa of rat P. carinii is the most frequent antigenic band reacting to human sera.

Citations

Citations to this article as recorded by

Human Immunodeficiency Virus-Infected Patients with PriorPneumocystisPneumonia Exhibit Increased Serologic Reactivity to Several Major Surface Glycoprotein Clones
K. R. Daly, J. V. Koch, N. J. Shire, L. Levin, P. D. Walzer
Clinical and Vaccine Immunology.2006; 13(10): 1071. CrossRef