Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.
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In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review Luis C. Ascanio, Savannah Carroll, Alberto Paniz-Mondolfi, Juan David Ramírez Frontiers in Microbiology.2024;[Epub] CrossRef
A refined genome phage display methodology delineates the human antibody response in patients with Chagas disease André Azevedo Reis Teixeira, Luis Rodriguez Carnero, Andréia Kuramoto, Fenny Hui Fen Tang, Carlos Hernique Gomes, Natalia Bueno Pereira, Léa Campos de Oliveira, Regina Garrini, Jhonatas Sirino Monteiro, João Carlos Setubal, Ester Cerdeira Sabino, Renata P iScience.2021; 24(6): 102540. CrossRef
Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
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