Parasites, Hosts and Diseases

"in vitro culture"

Original Article

In vitro culture of Cryptosporidium muris in a human stomach adenocarcinoma cell line: Min-Ho Choi, Sung-Tae Hong, Jong-Yil Chai, Woo-Yoon Park, Jae-Ran Yu; Korean J Parasitol 2004;42(1):27-34.
Published online March 20, 2004; DOI: https://doi.org/10.3347/kjp.2004.42.1.27

Abstract
PDF

We investigated the optimal culture conditions for Cryptosporidium muris in a human stomach adenocarcinoma (AGS) cell line by determining the effects of medium pH and of selected supplements on the development of C. muris. The optimum pH of the culture medium required for the development of C. muris was determined to be 6.6. The number of parasites significantly increased during cultivation for 72 hr (p < 0.05) at this level. On the other hand, numbers decreased linearly after 24 hr of incubation at pH 7.5. When cultured in different concentrations of serum, C. muris in media containing 5% FBS induced 4-7 times more parasites than in 1% or 10% serum. Of the six medium supplements examined, only 1 mM pyruvate enhanced the number of C. muris in vitro. Transmission electron microscopic observation showed the developmental stages of C. muris in the cytoplasm of the cells, not in an extracytoplasmic location. The growth of C. muris in AGS cells provides a means of investigating its biological characteristics and of testing its response to therapeutic agents. However, a more optimized culture system is needed for the recovery of oocysts on a large scale in vitro.

Citations

Citations to this article as recorded by

A Scoping Review of Changing Climate and Weather Connections to Waterborne Pathogens in Coastal Environments
Bailey M. Magers, Kyle D. Brumfield, Moiz Usmani, Rita R. Colwell, Antarpreet S. Jutla
Global Change Biology.2025;[Epub] CrossRef
Response of cell lines to actual and simulated inoculation with Cryptosporidium proliferans
Janka Melicherová, Lada Hofmannová, Andrea Valigurová
European Journal of Protistology.2018; 62: 101. CrossRef
The fine structure of sexual stage development and sporogony ofCryptosporidium parvumin cell-free culture
HEBATALLA M. ALDEYARBI, PANAGIOTIS KARANIS
Parasitology.2016; 143(6): 749. CrossRef
Morphology and infectivity of virus that persistently caused infection in an AGS cell line
Yukimasa Ooi, Eriko Daikoku, Hong Wu, Hiroaki Aoki, Chizuko Morita, Takashi Nakano, Takehiro Kohno, Tomohiko Takasaki, Kouichi Sano
Medical Molecular Morphology.2011; 44(4): 213. CrossRef
Evolution of Cryptosporidium in vitro culture
P. Karanis, H.M. Aldeyarbi
International Journal for Parasitology.2011; 41(12): 1231. CrossRef
Effect of select medium supplements on in vitro development of Cryptosporidium andersoni in HCT-8 cells
Liang Wu, Sheng-xia Chen, Xu-gan Jiang, Yu-juan Shen, Zhao-xi Lu, Guo-hua Tu, Xing-li Fu, Jian-ping Cao
Parasitology Research.2009; 105(5): 1419. CrossRef

8,095 View
77 Download
Crossref

Brief Communication

Postmetacercarial changes in Echinostoma caproni maintained in a defined medium plus calf serum: Bernard Fried, Aditya Reddy; Korean J Parasitol 2000;38(3):173-175.
Published online September 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.3.173

Abstract
PDF

The present study examined postmetacercarial changes in the excysted metacercariae of Echinostoma caproni maintained in the defined medium Mixture 199 plus 20% calf serum for 7 days at 41℃. The gas phase was atmospheric air. Each culture was inoculated with 25 excysted metacerariae. Cultures were maintained upright in closed 15 ml plastic centrifuge tubes each containing 10 ml of medium plus 200 units of penicillin/ml and 200 ?g of streptomycin/ml. By 4 days in culture, most metacercariae had voided their excretory concretions. Organisms were clumped or solitary at the bottom of the cultures. Many organisms showed flaring of the oral collar and extension of both the collar and tegumentary spines. By 4 days in culture, posterior protuberances or bumps were noted on many of the organisms and some organisms showed abnormal vesicular growths or blebs at their posterior ends. Some mortality was noted in culture by day 5, but most organisms were still alive when the cultures were terminated on day 7.

Citations

Citations to this article as recorded by

In vitro excystation of Echinostoma paraensei (Digenea: Echinostomatidae) metacercariae assessed by light microscopy, morphometry and confocal laser scanning microscopy
Joyce Souza, Juberlan Garcia, Renata H. Neves, José Roberto Machado-Silva, Arnaldo Maldonado
Experimental Parasitology.2013; 135(4): 701. CrossRef

7,133 View
66 Download
Crossref

Original Article

Effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae: M. Khyrul Islam, Kiku Matsuda, Jin-Ho Kim, Byeong-Kirl Baek; Korean J Parasitol 1999;37(1):13-19.
Published online March 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.1.13

Abstract
PDF

The effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae (L₃) to rats were investigated. A significantly higher body length was observed in L₃ from filter paper culture (597.3 ± 32.2 ?m) than those in fecal (509.9 ± 35.0 ?m) and nutrient broth culture (503.3 ± 31.0 ?m) (P<0.05). Larval infectivity was assessed by exposing rats to 1,000 L₃ from each culture and worms were recovered from the lungs and small intestines. Recovery rate of these worms did not show any significant difference. A significantly greater body length of adults was recorded in those corresponding to the L₃ harvested from filter paper (2,777.5 ± 204.4 ?m) and nutrient broth culture (2,732.5 ± 169.8 ?m) than those corresponding to the L₃ obtained from fecal culture (2,600.5 ± 172.4 ?m) (P<0.05). Although worm fecundity and EPG counts differed among culture methods but worm burdens and course of infection did not. These findings suggest that the methods of cultures have a significant effect on the morphological development of the larvae to the L₃ stage, but do not influence the infectivity to rats.

Citations

Citations to this article as recorded by

Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration
Daigo Tsubokawa, Taisei Kikuchi, Jae Man Lee, Takahiro Kusakabe, Yasuhiko Yamamoto, Haruhiko Maruyama, James B. Lok
PLOS Pathogens.2021; 17(6): e1009649. CrossRef
Strongyloides ratti and S. venezuelensis – rodent models of Strongyloides infection
MARK VINEY, TAISEI KIKUCHI
Parasitology.2017; 144(3): 285. CrossRef
Venestatin, a Ca ++ -binding protein from the parasitic nematode Strongyloides venezuelensis , is involved in the larval migration process
Daigo Tsubokawa, Takeshi Hatta, Taisei Kikuchi, Hiroki Maeda, Fusako Mikami, M. Abdul Alim, Haruhiko Maruyama, Naotoshi Tsuji
International Journal for Parasitology.2017; 47(8): 501. CrossRef
Viability of Strongyloides venezuelensis eggs and larvae in vermiculite containing the fungus Duddingtonia flagrans
Laryssa Pinheiro Costa Silva, Carolina Magri Ferraz, Anderson Rocha Aguiar, Jackson Victor Araújo, Steveen Rios Ribeiro, Débora Goldner Rossi, Luanderson Queiroz Mendes, Fausto Edmundo Lima Pereira, Narcisa Imaculada Brant Moreira, Fabio Ribeiro Braga
Parasitology Research.2017; 116(7): 2047. CrossRef
Development of free-living stages of Strongyloides ratti under different temperature conditions
Maki Sakamoto, Shoji Uga
Parasitology Research.2013; 112(12): 4009. CrossRef
Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis
Eiji Nagayasu, Yoshitoshi Ogura, Takehiko Itoh, Ayako Yoshida, Gunimala Chakraborty, Tetsuya Hayashi, Haruhiko Maruyama
Parasitology International.2013; 62(1): 57. CrossRef
Characterization of the protective response against a homologous challenge infection with Strongyloides venezuelensis in rats
B.K Baek, M.K Islam, B.S Kim, C.W Lim, J Hur, A.O Oluoch, C.H Kim, I Kakoma
Veterinary Parasitology.2003; 113(3-4): 217. CrossRef
Persistent infection with Strongyloides venezuelensis in the Mongolian gerbil (Meriones unguiculatus)
Byeong-Kirl Baek, In-Soo Whang, M. Khyrul Islam, Byeong-Soo Kim, Ibulaimu Kakoma
The Korean Journal of Parasitology.2002; 40(4): 181. CrossRef
Partial cross-resistance between Strongyloides venezuelensis and Nippostrongylus brasiliensis in rats
Byeong-Kirl Baek, M. Khyrul Islam, Jin-Ho Kim, John-Wha Lee, Jin Hur
The Korean Journal of Parasitology.1999; 37(2): 101. CrossRef