In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.
Citations
Citations to this article as recorded by
A point-of-care testing assay for clonorchiasis using a EuNPs-CsTR1 fluorescent probe-based immunoassay Xiaoxiao Ma, Huiyuan Zhang, Yiming Fang, Jing Wang, Xingyang Wang, Chen Li, Xiaolei Liu, Mingyuan Liu, Bin Tang, Yi Liu, Javier Sotillo PLOS Neglected Tropical Diseases.2024; 18(4): e0012107. CrossRef
Evaluation of Crude and Recombinant Antigens of Schistosoma japonicum for the Detection of Schistosoma mekongi Human Infection Jose Ma. M. Angeles, Atcharaphan Wanlop, Minh-Anh Dang-Trinh, Masashi Kirinoki, Shin-ichiro Kawazu, Aya Yajima Diagnostics.2023; 13(2): 184. CrossRef
Combination of Biodata Mining and Computational Modelling in Identification and Characterization of ORF1ab Polyprotein of SARS-CoV-2 Isolated from Oronasopharynx of an Iranian Patient Reza Zolfaghari Emameh, Hassan Nosrati, Ramezan Ali Taheri Biological Procedures Online.2020;[Epub] CrossRef
Cs1, a Clonorchis sinensis-derived serodiagnostic antigen containing tandem repeats and a signal peptide Na Cheng, Xue-Nian Xu, Yan Zhou, Yu-Ting Dong, Yi-Fang Bao, Bin Xu, Wei Hu, Zheng Feng, Maria Elena Bottazzi PLOS Neglected Tropical Diseases.2018; 12(8): e0006683. CrossRef
Heat shock proteins 70 and 90 from Clonorchis sinensis induce Th1 response and stimulate antibody production Eun Joo Chung, Young-Il Jeong, Myoung-Ro Lee, Yu Jung Kim, Sang-Eun Lee, Shin-Hyeong Cho, Won-Ja Lee, Mi-Yeoun Park, Jung-Won Ju Parasites & Vectors.2017;[Epub] CrossRef
Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in Clonorchis sinensis as a Potential Druggable Target Yu-Jung Kim, Won Yoo, Myoung-Ro Lee, Jung-Mi Kang, Byoung-Kuk Na, Shin-Hyeong Cho, Mi-Yeoun Park, Jung-Won Ju International Journal of Molecular Sciences.2017; 18(3): 557. CrossRef
Development of “-omics†research in Schistosoma spp. and -omics-based new diagnostic tools for schistosomiasis Shuqi Wang, Wei Hu Frontiers in Microbiology.2014;[Epub] CrossRef
Analysis of an expressed sequence tag library from Dicrocoelium dentriticum A.M. Martínez-Ibeas, M.J. Perteguer, C. González-Lanza, T. Gárate, M.Y. Manga-González Experimental Parasitology.2013; 135(2): 287. CrossRef
Genomics and molecular genetics of Clonorchis sinensis: Current status and perspectives Si-Yang Huang, Guang-Hui Zhao, Bao-Quan Fu, Min-Jun Xu, Chun-Ren Wang, Song-Ming Wu, Feng-Cai Zou, Xing-Quan Zhu Parasitology International.2012; 61(1): 71. CrossRef
Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong Parasitology Research.2011; 108(5): 1295. CrossRef
Human Antibody Response to Thioredoxin Peroxidase-1 and Tandem Repeat Proteins as Immunodiagnostic Antigen Candidates for Schistosoma japonicum Infection Shin-ichiro Kawazu, Masashi Kirinoki, Pilarita Tongol-Rivera, Yasuyuki Goto, Lydia Leonardo, Elena Villacorte, Yuichi Chigusa, Noboru Inoue, Jose Ma. Angeles The American Journal of Tropical Medicine and Hygiene.2011; 85(4): 674. CrossRef
Recent Advances in Serodiagnosis for Clonorchiasis Tae Im Kim, Sung-Jong Hong Hanyang Medical Reviews.2010; 30(3): 232. CrossRef
Functional Genes and Proteins of Clonorchis sinensis Tae Im Kim, Byoung-Kuk Na, Sung-Jong Hong The Korean Journal of Parasitology.2009; 47(Suppl): S59. CrossRef
Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α Tae Yun Kim, Pyo Yun Cho, Jong Won Na, Sung-Jong Hong Parasitology Research.2007; 101(6): 1557. CrossRef
Clonorchiasis: an update H.-J. Rim Journal of Helminthology.2005; 79(3): 269. CrossRef
Immunology and molecular biology of Opisthorchis viverrini infection S. Wongratanacheewin, R.W. Sermswan, S. Sirisinha Acta Tropica.2003; 88(3): 195. CrossRef
Use of a recombinant Clonorchis sinensis pore-forming peptide, clonorin, for serological diagnosis of clonorchiasis Ji-Yun Lee, Tae Yun Kim, Xiao-Xian Gan, Shin-Yong Kang, Sung-Jong Hong Parasitology International.2003; 52(2): 175. CrossRef
Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach Ji-Sook Lee, Jongweon Lee, Soon-Jung Park, Tai-Soon Yong Parasitology Research.2003; 91(4): 283. CrossRef
First
Prev
