Parasites, Hosts and Diseases

"solution"

Original Articles

Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii: Eun-Kyung Moon, Hye-Ryun Park, Fu-Shi Quan, Hyun-Hee Kong; Korean J Parasitol 2016;54(6):697-702.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.697

Abstract
PDF

Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing and disinfecting microorganisms because of its convenience. We determined amoebicidal effects of MPDS made in Korea and their cytotoxicity on human corneal epithelium cells. Fifteen commercial MPDS (A to O) were tested for their amoebicidal effects on Acanthamoeba castellanii trophozoites and cysts by using a most probable number (MPN) technique. Among them, 7 kinds of MPDS showed little or no amoebicidal effects for 24 hr exposure. Solutions A, B, G, H, L, and O showed positive amoebicidal effects, and solutions M and N killed almost all trophozoites and cysts after 24 hr exposure. However, 50%-N solution showed 56% cytotoxicity on human corneal epithelial cells within 4 hr exposure, and 50%-O solution also showed 62% cytotoxicity on human cells within 4 hr exposure. Solution A did not show any cytotoxicity on human cells. These results revealed that most MPDS made in Korea were ineffective to kill Acanthamoeba. The solutions having amoebicidal activity also showed high levels of cytotoxicity on human corneal epithelial cells. New formulations for improved MPDS that are amoebicidal but safe for host cells are needed to prevent Acanthamoeba keratitis.

Citations

Citations to this article as recorded by

Amoebicidal and cysticidal in vitro activity of cationic dendritic molecules against Acanthamoeba polyphaga and Acanthamoeba griffini
Cristina Verdú-Expósito, Tania Martín-Pérez, Jorge Pérez-Serrano, Javier Sanchez-Nieves, Francisco Javier de la Mata, Irene Heredero-Bermejo
Parasitology Research.2024;[Epub] CrossRef
Tannic Acid-Modified Silver Nanoparticles in Conjunction with Contact Lens Solutions Are Useful for Progress against the Adhesion of Acanthamoeba spp. to Contact Lenses
Marcin Padzik, Lidia Chomicz, Julita Bluszcz, Karolina Maleszewska, Jaroslaw Grobelny, David Bruce Conn, Edyta B. Hendiger
Microorganisms.2022; 10(6): 1076. CrossRef
In vitro effects of multi-purpose contact lens disinfecting solutions towards survivability of Acanthamoeba genotype T4 in Malaysia
Rosnani Hanim Mohd Hussain, Wan Nur Afiqah, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
Saudi Journal of Biological Sciences.2021; 28(4): 2352. CrossRef
Development of anti-acanthamoebic approaches
Mohammad Ridwane Mungroo, Tommy Tong, Naveed Ahmed Khan, Tengku Shahrul Anuar, Sutherland K. Maciver, Ruqaiyyah Siddiqui
International Microbiology.2021; 24(3): 363. CrossRef
BCLA CLEAR - Contact lens wettability, cleaning, disinfection and interactions with tears
Mark Willcox, Nancy Keir, Vinod Maseedupally, Simin Masoudi, Alison McDermott, Rabia Mobeen, Christine Purslow, Jacinto Santodomingo-Rubido, Silvia Tavazzi, Fabrizio Zeri, Lyndon Jones
Contact Lens and Anterior Eye.2021; 44(2): 157. CrossRef
In Vitro Evaluation of the Combination of Melaleuca alternifolia (Tea Tree) Oil and Dimethyl Sulfoxide (DMSO) against Trophozoites and Cysts of Acanthamoeba Strains. Oxygen Consumption Rate (OCR) Assay as a Method for Drug Screening
Tania Martín-Pérez, Irene Heredero-Bermejo, Cristina Verdú-Expósito, Jorge Pérez-Serrano
Pathogens.2021; 10(4): 491. CrossRef
Combination of tert-butyl hydroperoxide with vorinostat induces cell death of Acanthamoeba through cell cycle arrest
Hae-Ahm Lee, Eun-Kyung Moon, Fu-Shi Quan
Experimental Parasitology.2020; 210: 107833. CrossRef
Anti-staphylococcal Effect of a Nephrite-containing Contact Lens Storage Case
Su Hwan Park, Seung Uk Lee, Yoon Kyung Kim, Hak Sun Yu, Sung Hee Park, Jung Hyo Ahn, Su Jin Kim, Jong Hoon Shin, Ji Eun Lee
Journal of the Korean Ophthalmological Society.2020; 61(8): 868. CrossRef
Synthesis and in vitro activity of new biguanide-containing dendrimers on pathogenic isolates of Acanthamoeba polyphaga and Acanthamoeba griffini
T. Martín-Pérez, T. Lozano-Cruz, A. Criado-Fornelio, P. Ortega, R. Gómez, F. J. de la Mata, J. Pérez-Serrano
Parasitology Research.2019; 118(6): 1953. CrossRef
Chloroquine as a possible disinfection adjunct of disinfection solutions against Acanthamoeba
Eun-Kyung Moon, Seungeun Lee, Fu-Shi Quan, Hyun-Hee Kong
Experimental Parasitology.2018; 188: 102. CrossRef
Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions
Eun-Kyung Moon, Seungeun Lee, Fu-Shi Quan, Hyun-Hee Kong
The Korean Journal of Parasitology.2018; 56(5): 491. CrossRef
Amoebicidal Effect of Nephrite-containing Contact Lens Storage Case
Jae Woo Jung, Jong Heon Lee, Sung Hee Park, Hak Sun Yu, Yoon Kyung Kim, Ji-Eun Lee
Journal of the Korean Ophthalmological Society.2017; 58(5): 509. CrossRef
Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae
Hae-Jin Sohn, Heekyoung Kang, Ga-Eun Seo, Jong-Hyun Kim, Suk-Yul Jung, Ho-Joon Shin
The Korean Journal of Parasitology.2017; 55(3): 233. CrossRef
Effect of Multipurpose Solution Combined With Autophagy Inhibitors on Adhesion of Acanthamoeba trophozoites to Silicone Hydrogel Contact Lenses
Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee
Cornea.2017; 36(12): 1538. CrossRef

9,776 View
229 Download
14 Web of Science
Crossref

Molecular Differentiation of Opisthorchis viverrini and Clonorchis sinensis Eggs by Multiplex Real-Time PCR with High Resolution Melting Analysis: Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Porntip Laummaunwai, Viraphong Lulitanond, Pham Ngoc Doanh, Wanchai Maleewong; Korean J Parasitol 2013;51(6):689-694.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.689

Abstract
PDF

Opisthorchis viverrini and Clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in Asia. The standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. However, the method has low sensitivity, and eggs of O. viverrini and C. sinensis are difficult to distinguish from each other and from those of some other trematodes. Here, we report a multiplex real-time PCR coupled with high resolution melting (HRM) analysis for the differentiation of O. viverrini and C. sinensis eggs in fecal samples. Using 2 pairs of species-specific primers, DNA sequences from a portion of the mitochondrial NADH dehydrogenase subunit 2 (nad 2) gene, were amplified to generate 209 and 165 bp products for O. viverrini and C. sinensis, respectively. The distinct characteristics of HRM patterns were analyzed, and the melting temperatures peaked at 82.4±0.09℃ and 85.9±0.08℃ for O. viverrini and C. sinensis, respectively. This technique was able to detect as few as 1 egg of O. viverrini and 2 eggs of C. sinensis in a 150 mg fecal sample, which is equivalent to 7 and 14 eggs per gram of feces, respectively. The method is species-specific, rapid, simple, and does not require fluorescent probes or post-PCR processing for discrimination of eggs of the 2 species. It offers a new tool for differentiation and detection of Asian liver fluke infections in stool specimens.

Citations

Citations to this article as recorded by

Multiparasitism in Spain in a Korean Visiting Friends and Relatives: Case Report and Review of Imported Cases of Clonorchis sinensis in Europe
Paola Cociancic, Jacklyn Comas, J. Guillermo Esteban
Clinical Case Reports.2025;[Epub] CrossRef
Study of the population genetic structure of Opisthorchis-like eggs in northern Thailand using mitochondrial genes
Picha Suwannahitatorn, Mathirut Mungthin, Ittisak Subrungruang, Lakhanawan Charoensuk, Nithikoon Aksorn, Saiwasan Buathong, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2024; 18(8): e0012445. CrossRef
An Approach for Egg Parasite Classification Based on Ensemble Deep Learning
Narut Butploy, Wanida Kanarkard, Pewpan M. Intapan, Oranuch Sanpool
Journal of Advanced Computational Intelligence and Intelligent Informatics.2023; 27(6): 1113. CrossRef
Fish and Food-Fatale: Food-borne Trematode Opisthorchis viverrini and Cholangiocarcinoma
S. Tan, M. Machrumnizar
Helminthologia.2023; 60(4): 287. CrossRef
Are Melanoides tuberculata and Tarebia granifera (Gastropoda, Thiaridae), suitable first intermediate hosts of Clonorchis sinensis in Vietnam?
Hung Manh Nguyen, Hien Hoang Van, Loan Thi Ho, Yulia V. Tatonova, Henry Madsen, Xiao-Nong Zhou
PLOS Neglected Tropical Diseases.2021; 15(1): e0009093. CrossRef
Clonorchis sinensis and clonorchiasis
Byoung-Kuk Na, Jhang Ho Pak, Sung-Jong Hong
Acta Tropica.2020; 203: 105309. CrossRef
Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics
Pyo Yun Cho, Ji-Yun Lee, Tae Im Kim, Jin-Ho Song, Sung-Jong Hong, Won Gi Yoo, Takafumi Tsuboi, Kwon-Soo Ha, Jae-Wan Jung, Satoru Takeo, Eun-Taek Han, Banchob Sripa, Sung-Tae Hong, Jong-Yil Chai, Ho-Woo Nam, Jhang Ho Pak, Tong-Soo Kim, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2020; 14(12): e0008998. CrossRef
Clonorchiasis sinensis detected by laparoscopic exploration of biliary tracts in two patients with obstructive jaundice
Xialei Liu, Genglong Zhu, Chaonong Cai, Zhiyue Lv, Jian Li
BMC Infectious Diseases.2019;[Epub] CrossRef
Development of Conventional Multiplex PCR: A Rapid Technique for Simultaneous Detection of Soil-Transmitted Helminths
Vivornpun Sanprasert, Ruthairat Kerdkaew, Siriporn Srirungruang, Sarit Charuchaibovorn, Kobpat Phadungsaksawasdi, Surang Nuchprayoon
Pathogens.2019; 8(3): 152. CrossRef
Performance evaluation of existing immunoassays for Clonorchis sinensis infection in China
Hong-Mei Li, Men-Bao Qian, Yi-Chao Yang, Zhi-Hua Jiang, Kang Wei, Jia-Xu Chen, Jun-Hu Chen, Ying-Dan Chen, Xiao-Nong Zhou
Parasites & Vectors.2018;[Epub] CrossRef
Molecular characterization of the unique Mesostephanus appendiculatus (Trematoda: Cyathocotylidae) by small ribosomal RNA from Egypt
Nasr M. El-Bahy, Eman K. Bazh, Shimaa S. Sorour, Nagwa M. Elhawary
Parasitology Research.2017; 116(4): 1129. CrossRef
LAMPhimerus: A novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples
William Cevallos, Pedro Fernández-Soto, Manuel Calvopiña, Cristina Fontecha-Cuenca, Hiromu Sugiyama, Megumi Sato, Julio López Abán, Belén Vicente, Antonio Muro, David Blair
PLOS Neglected Tropical Diseases.2017; 11(6): e0005672. CrossRef
Molecular discrimination of Opisthorchis-like eggs from residents in a rural community of central Thailand
Saiwasan Buathong, Saovanee Leelayoova, Mathirut Mungthin, Toon Ruang-areerate, Tawee Naaglor, Picha Suwannahitatorn, Phunlerd Piyaraj, Paanjit Taamasri, Peerapan Tan-ariya, Edoardo Pozio
PLOS Neglected Tropical Diseases.2017; 11(11): e0006030. CrossRef
Current status ofClonorchis sinensisand clonorchiasis in China
De-Hua Lai, Xiao-Kun Hong, Bi-Xiu Su, Chi Liang, Geoff Hide, Xiaoli Zhang, Xinbing Yu, Zhao-Rong Lun
Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(1): 21. CrossRef

10,882 View
131 Download
Crossref

Molecular Differentiation of Schistosoma japonicum and Schistosoma mekongi by Real-Time PCR with High Resolution Melting Analysis: Amornmas Kongklieng, Worasak Kaewkong, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Viraphong Lulitanond, Pusadee Sri-Aroon, Yanin Limpanont, Wanchai Maleewong; Korean J Parasitol 2013;51(6):651-656.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.651

Abstract
PDF

Human schistosomiasis caused by Schistosoma japonicum and Schistosoma mekongi is a chronic and debilitating helminthic disease still prevalent in several countries of Asia. Due to morphological similarities of cercariae and eggs of these 2 species, microscopic differentiation is difficult. High resolution melting (HRM) real-time PCR is developed as an alternative tool for the detection and differentiation of these 2 species. A primer pair was designed for targeting the 18S ribosomal RNA gene to generate PCR products of 156 base pairs for both species. The melting points of S. japonicum and S. mekongi PCR products were 84.5±0.07℃ and 85.7±0.07℃, respectively. The method permits amplification from a single cercaria or an egg. The HRM real-time PCR is a rapid and simple tool for differentiation of S. japonicum and S. mekongi in the intermediate and final hosts.

Citations

Citations to this article as recorded by

Current advances in serological and molecular diagnosis of Schistosoma mekongi infection
Adrian Miki C. Macalanda, Atcharaphan Wanlop, Kevin Austin L. Ona, Eloiza May S. Galon, Virak Khieu, Somphou Sayasone, Aya Yajima, Jose Ma. M. Angeles, Shin-ichiro Kawazu
Tropical Medicine and Health.2024;[Epub] CrossRef
Molecular Techniques as Alternatives of Diagnostic Tools in China as Schistosomiasis Moving towards Elimination
Chao Lv, Wangping Deng, Liping Wang, Zhiqiang Qin, Xiaonong Zhou, Jing Xu
Pathogens.2022; 11(3): 287. CrossRef
Development of a novel real-time polymerase chain reaction assay for the sensitive detection of Schistosoma japonicum in human stool
Sara Halili, Jessica R. Grant, Nils Pilotte, Catherine A. Gordon, Steven A. Williams, Cinzia Cantacessi
PLOS Neglected Tropical Diseases.2021; 15(10): e0009877. CrossRef
Establishment and application of a novel fluorescence-based analytical method for the rapid detection of viable bacteria in different samples
Qiuyue Yin, Maiqian Nie, Zhenjun Diwu, Yuting Zhang, Lei Wang, Dandan Yin, Liancheng Li
Analytical Methods.2020; 12(31): 3933. CrossRef
Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+-dependent cysteine protease (calpain)
Salisa Chaimon, Yanin Limpanont, Onrapak Reamtong, Sumate Ampawong, Orawan Phuphisut, Phiraphol Chusongsang, Jiraporn Ruangsittichai, Usa Boonyuen, Dorn Watthanakulpanich, Anthony J. O’Donoghue, Conor R. Caffrey, Poom Adisakwattana
Parasites & Vectors.2019;[Epub] CrossRef
Rapid detection and identification of four major Schistosoma species by high-resolution melt (HRM) analysis
Juan Li, Guang-Hui Zhao, RuiQing Lin, David Blair, Hiromu Sugiyama, Xing-Quan Zhu
Parasitology Research.2015; 114(11): 4225. CrossRef
Detection of Schistosoma mansoni and Schistosoma haematobium by Real-Time PCR with High Resolution Melting Analysis
Hany Sady, Hesham Al-Mekhlafi, Romano Ngui, Wahib Atroosh, Ahmed Al-Delaimy, Nabil Nasr, Salwa Dawaki, Awatif Abdulsalam, Init Ithoi, Yvonne Lim, Kek Chua, Johari Surin
International Journal of Molecular Sciences.2015; 16(7): 16085. CrossRef
Bacterial detection: From microscope to smartphone
Subash C.B. Gopinath, Thean-Hock Tang, Yeng Chen, Marimuthu Citartan, Thangavel Lakshmipriya
Biosensors and Bioelectronics.2014; 60: 332. CrossRef

10,177 View
104 Download
Crossref

Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P: Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.645

Abstract
PDF

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

Citations

Citations to this article as recorded by

Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis
Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
Pathogens.2024; 13(6): 447. CrossRef
Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods
Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
Pathogens.2024; 13(11): 950. CrossRef
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM
Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto
Journal of Vocational Health Studies.2024; 8(1): 42. CrossRef
Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of Anopheles and Aedes Surveillance Strategies
Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar
Pathogens.2023; 12(12): 1406. CrossRef
Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting)
Bagus Muhammad Ihsan, Afiat Berbudi, Ridad Agoes, Muhammad Ifham Hanif
Acta Parasitologica.2022; 67(1): 496. CrossRef
Collection and DNA Detection of Dirofilaria immitis (Rhabditida Onchocercidae), Using a Novel Primer Set, in Wild-Caught Mosquitoes From Gainesville, FL
Chris Holderman, Nicole O Abruzzo, Noor A Abdelsamad, Phillip E Kaufman, Peter M DiGennaro, Stephen Rich
Journal of Medical Entomology.2021; 58(3): 1429. CrossRef
The use of molecular xenomonitoring for surveillance of mosquito-borne diseases
Mary M. Cameron, Anita Ramesh
Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816. CrossRef
Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia
Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto
Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef
The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria
Karin Sonnberger, Hans-Peter Fuehrer, Bernhard Werner Sonnberger, Michael Leschnik
Pathogens.2021; 10(5): 550. CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
Bagus Muhammad Ihsan, Cecep Dani Sucipto, Khayan Khayan
Journal of Vocational Health Studies.2021; 5(1): 26. CrossRef
Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences
Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi
Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef
Prevalence and risk factors associated with Dirofilaria immitis infection in dogs and cats in Songkhla and Satun provinces, Thailand
Ketsarin Kamyingkird, Witchuta Junsiri, Witsanuwat Chimnoi, Chanya Kengradomkij, Sinsamut Saengow, Keplee Sangchuto, Wicha Kajeerum, Decha Pangjai, Burin Nimsuphan, Tawin Inpankeaw, Sathaporn Jittapalapong
Agriculture and Natural Resources.2017; 51(4): 299. CrossRef
Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?
Aleksander Masny, Rusłan Sałamatin, Wioletta Rozej-Bielicka, Elzbieta Golab
Parasitology Research.2016; 115(2): 511. CrossRef
Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes
Oranuch Sanpool, Chairat Tantrawatpan, Tongjit Thanchomnang, Penchom Janwan, Pewpan M. Intapan, Rutchanee Rodpai, Viraphong Lulitanond, Piyanan Taweethavonsawat, Wanchai Maleewong
Vector-Borne and Zoonotic Diseases.2016; 16(5): 326. CrossRef
Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction
Jurairat Jongthawin, Pewpan M. Intapan, Viraphong Lulitanond, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Wanchai Maleewong
Parasitology Research.2016; 115(8): 2967. CrossRef
Trypanosome infection rates in tsetse flies in the “silent” sleeping sickness focus of Bafia in the Centre Region in Cameroon
Gustave Simo, Pierre Fongho, Oumarou Farikou, Prosper Innocent Ndjeuto Ndjeuto-Tchouli, Judith Tchouomene-Labou, Flobert Njiokou, Tazoacha Asonganyi
Parasites & Vectors.2015;[Epub] CrossRef

11,665 View
134 Download
Crossref

In Vitro Maintenance of Clonorchis sinensis Adult Worms: Md. Hafiz Uddin, Shunyu Li, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2012;50(4):309-315.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.309

Abstract
PDF

Clonorchis sinensis is a biological carcinogen inducing human cholangiocarcinoma, and clonorchiasis is one of the important endemic infectious diseases in East Asia. The present study investigated survival longevity of C. sinensis adult worms in various in vitro conditions to find the best way of keeping the worms longer. The worms were maintained in 0.85% NaCl, 1×PBS, 1×Locke's solution, RPMI-1640, DMEM, and IMDM media, and in 1×Locke's solution with different supplements. All of the worms died within 3 and 7 days in 0.85% NaCl and 1×PBS, respectively, but survived up to 57 days in 1×Locke's solution. The worms lived for 106 days in DMEM, and 114 days in both RPMI-1640 and IMDM media. The survival rate in RPMI-1640 medium was the highest (50%) compared to that in DMEM (20±10%) and in IMDM (33.3±25.2%) after 3 months. The 1×Locke's solution with 0.005% bovine bile supplement showed increased duration of maximum survival from 42 days to 70 days. Higher concentration of bile supplements than 0.005% or addition of glucose were disadvantageous for the worm survival. The worms died rapidly in solutions containing L-aspartic acid, L-glutamic acid, and adenine compared to L-arginine, L-serine, and L-tryptophan. In conclusion, the 1×Locke's solution best supports the worms alive among inorganic solutions for 57 days, and the RPMI-1640 medium maintains living C. sinensis adults better and longer up to 114 days in vitro than other media.

Citations

Citations to this article as recorded by

Clonorchis sinensis and Cholangiocarcinoma
Eun-Min Kim, Sung-Tae Hong
Journal of Korean Medical Science.2025;[Epub] CrossRef
The Truman Show for Human Helminthic Parasites: A Review of Recent Advances in In Vitro Cultivation Platforms
Smita Sutrave, Martin Heinrich Richter
Microorganisms.2023; 11(7): 1708. CrossRef
In vitro culture and morphology of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) collected from the intestine of tambaqui (Colossoma macropomum) farmed in the Brazilian Amazon
M. I. B. Oliveira, C. Majolo, F. A. Sebastião, M. Tavares-Dias, F. R. Brandão, E. C. Chagas
Brazilian Journal of Biology.2023;[Epub] CrossRef
Structure-based virtual screening and molecular dynamics of potential inhibitors targeting sodium-bile acid co-transporter of carcinogenic liver fluke Clonorchis sinensis
Won Gi Yoo, Fuhong Dai, Jhang Ho Pak, Sung-Jong Hong, Jin-Ho Song, Sutas Suttiprapa
PLOS Neglected Tropical Diseases.2022; 16(11): e0010909. CrossRef
Amino acids serve as an important energy source for adult flukes of Clonorchis sinensis
Shan Li, Xueqing Chen, Juanjuan Zhou, Zhizhi Xie, Mei Shang, Lei He, Pei Liang, Tingjin Chen, Qiang Mao, Chi Liang, Xuerong Li, Yan Huang, Xinbing Yu, John Pius Dalton
PLOS Neglected Tropical Diseases.2020; 14(4): e0008287. CrossRef
Sodium-bile acid co-transporter is crucial for survival of a carcinogenic liver fluke Clonorchis sinensis in the bile
Fuhong Dai, Won Gi Yoo, Yanyan Lu, Jin-Ho Song, Ji-Yun Lee, Youngro Byun, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
PLOS Neglected Tropical Diseases.2020; 14(12): e0008952. CrossRef
Characterization of a novel organic solute transporter homologue from Clonorchis sinensis
Yanyan Lu, Won Gi Yoo, Fuhong Dai, Ji-Yun Lee, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong, John Pius Dalton
PLOS Neglected Tropical Diseases.2018; 12(4): e0006459. CrossRef
Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis, a 53.1-kDa homopentamer, implicated immune protective efficacy against clonorchiasis
Tingjin Chen, Hongye Jiang, Hengchang Sun, Zhizhi Xie, Pengli Ren, Lu Zhao, Huimin Dong, Mengchen Shi, Zhiyue Lv, Zhongdao Wu, Xuerong Li, Xinbing Yu, Yan Huang, Jin Xu
Parasites & Vectors.2017;[Epub] CrossRef
C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine
Md. Hafiz Uddin, Shunyu Li, Yan Jin, Min-Ho Choi, Ja June Jang, Sung-Tae Hong
The Korean Journal of Parasitology.2016; 54(3): 281. CrossRef
Clonorchis sinensis acetoacetyl-CoA thiolase: identification and characterization of its potential role in surviving in the bile duct
Jinsi Lin, Hongling Qu, Guishan Chen, Lei He, Yanquan Xu, Zhizhi Xie, Mengyu Ren, Jiufeng Sun, Shan Li, Wenjun Chen, Xueqing Chen, Xiaoyun Wang, Xuerong Li, Chi Liang, Yan Huang, Xinbing Yu
Parasites & Vectors.2015;[Epub] CrossRef
Involvement of PSMD10, CDK4, and Tumor Suppressors in Development of Intrahepatic Cholangiocarcinoma of Syrian Golden Hamsters Induced by Clonorchis sinensis and N-Nitrosodimethylamine
Md. Hafiz Uddin, Min-Ho Choi, Woo Ho Kim, Ja-June Jang, Sung-Tae Hong, Xiao-Nong Zhou
PLOS Neglected Tropical Diseases.2015; 9(8): e0004008. CrossRef
Advanced Enzymology, Expression Profile and Immune Response of Clonorchis sinensis Hexokinase Show Its Application Potential for Prevention and Control of Clonorchiasis
Tingjin Chen, Jinyun Yu, Zeli Tang, Zhizhi Xie, Zhipeng Lin, Hengchang Sun, Shuo Wan, Xuerong Li, Yan Huang, Xinbing Yu, Jin Xu, Aaron R. Jex
PLOS Neglected Tropical Diseases.2015; 9(3): e0003641. CrossRef
Production and Deformation of Clonorchis sinensis Eggs during In Vitro Maintenance
Md. Hafiz Uddin, Young Mee Bae, Min-Ho Choi, Sung-Tae Hong, Jason Mulvenna
PLoS ONE.2012; 7(12): e52676. CrossRef

10,792 View
110 Download
Crossref

Brief Communication

In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst: Seyed Vahid Hosseini, Kurosh Ghanbarzadeh, Zahra Barzin, Seyed Mahmoud Sadjjadi, Nader Tanideh, Davood Mehrabani; Korean J Parasitol 2006;44(3):239-242.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.239

Abstract
PDF

To evaluate the protoscolicidal effects of various concentrations of hypertonic glucose, live protoscolices of sheep were exposed to 10%, 15%, 25% and 50% glucose solutions. Cetrimide (0.5%), silver nitrate (0.5%) and hypertonic saline (20%) were used as positive controls, while physiological saline was used as a negative control. After 1, 2 and 5 min, the protoscolicidal effects were determined by 1% eosin. A 25% glucose solution had no significant protoscolicidal effect. However, a 50% glucose solution revealed higher protoscolicidal effect than 0.5% silver nitrate but weaker effect than 0.5% cetrimide; the effect was comparable with that of 20% hypertonic saline. The results showed that hypertonic glucose solution is highly effective in killing protoscolices of Echinococcus granulosus in vitro.

Citations

Citations to this article as recorded by

In Vitro Scolicidal Effect of Urtica dioica and Pyrus boissieriana Extracts Against Protoscoleces of Echinococcus granulosus
Kimia Goudarzi, Rasoul Alimi, Tandis Zarghami, Mohammad Saleh Bahreini, Mohamad Ghanimatdan, Meysam Sharifdini, Erfan Kohansal, Aref Teimouri
Shiraz E-Medical Journal.2024;[Epub] CrossRef
Protoscolicidal effects of curcumin nanoemulsion against protoscoleces of Echinococcus granulosus
Aref Teimouri, Sanaz Jafarpour Azami, Saeedeh Hashemi Hafshejani, Mohammad Ghanimatdan, Mohammad Saleh Bahreini, Rasoul Alimi, Seyed Mahmoud Sadjjadi
BMC Complementary Medicine and Therapies.2023;[Epub] CrossRef
Scorpion crude venom induced apoptosis and structural changes of Echinococcus granulosus protoscolices
Esam S. Al-Malki, Manei M. Aljedaie, Omar S.O. Amer, Naser Abdelsater, Ahmed Badry
Journal of King Saud University - Science.2022; 34(4): 101937. CrossRef
In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism
Ya Xu, Wenjuan Qing, Zhen Wang, Lin Chen, Linyao Wang, Hailong Lv, Yufeng Jiang
Experimental Parasitology.2022; 239: 108280. CrossRef
Anti-hydatidosis Potential of Sodium Valproate: Evaluating New Therapeutic Potential for Sodium Valproate
Mohammadreza Rezaie, Reza Rahimi, Zahra Eslamirad
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef
Study on the effect of an ion channel inhibitor “Fluralaner” on Echinococcus granulosus protoscolices and metacestode layers in vitro
Fatima Zahran, Hayam Mohamed Ezz El-Din, Mai Abdel Sameaa Shehata
Journal of Parasitic Diseases.2020; 44(2): 411. CrossRef
Echinococcus Granulosus in Childhood: A Retrospective Study of 187 Cases and Newer Data
Anastasios S. Petropoulos, Georgios A. Chatzoulis
Clinical Pediatrics.2019; 58(8): 864. CrossRef
In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts
Nasr Moawad El-Bahy, Amer Ragheb Abdelaziz, Reda Elbastawisy Khalafalla
Revista Brasileira de Parasitologia Veterinária.2019; 28(2): 210. CrossRef
In Vitro Study on Protoscolicidal Effect of Methanolic Extract of Allium hirtifolium on Protoscoleces of Cystic Echinococcosis
Z. Shahamir Tabatabaei, S. Dehshahri, M.M. Taghi, F. Esfandiari, F.S. Sadjjadi, M. Ebrahimipour, S.M. Sadjjadi
Infectious Disorders - Drug Targets.2019; 19(3): 264. CrossRef
Povidone iodine versus silver nitrate in induction of sclerosing cholangitis in hepatic hydatid cysts surgery
Seyed Vahid Hosseini, Abdulrazzak Kalaf Hassan, Ahmed Mohamed Ali Hussein Alhurry, Maral Mokhtari, Nader Tanideh, Sepideh Sefidbakht, Hajar Khazraei
Comparative Clinical Pathology.2018; 27(1): 239. CrossRef
Solidification of hydatid cyst fluid with an injectable chitosan/carboxymethylcellulose/β-glycerophosphate hydrogel for effective control of spillage during aspiration of hydatid cysts
Mostafa D. A. Azadi, Shadi Hassanjili, Khalil Zarrabi, Bahador Sarkari
Progress in Biomaterials.2018; 7(1): 35. CrossRef
Progress in the pharmacological treatment of human cystic and alveolar echinococcosis: Compounds and therapeutic targets
Mar Siles-Lucas, Adriano Casulli, Roberto Cirilli, David Carmena, Giovanna Raso
PLOS Neglected Tropical Diseases.2018; 12(4): e0006422. CrossRef
Chemical composition and scolicidal activity of Zataria multiflora Boiss essential oil
Hossein Mahmoudvand, Seyed Reza Mirbadie, Saeed Sadooghian, Majid Fasihi Harandi, Sareh Jahanbakhsh, Ebrahim Saedi Dezaki
Journal of Essential Oil Research.2017; 29(1): 42. CrossRef
Effects of trigonelline inhibition of the Nrf2 transcription factor in vitro on Echinococcus granulosus
Wenjuan Qin, Dongfang Guan, Rongji Ma, Rentan Yang, Guoqiang Xing, Hongjuan Shi, Guangyao Tang, Jiajie Li, Hailong Lv, Yufeng Jiang
Acta Biochimica et Biophysica Sinica.2017; 49(8): 696. CrossRef
Scolicidal agents for protoscolices of Echinococcus granulosus hydatid cyst: Review of literature
Seyedeh Maryam Sharafi, Raheleh Rafiei Sefiddashti, Behnam Sanei, Morteza Yousefi, Hossein Yousofi Darani
Journal of Research in Medical Sciences.2017;[Epub] CrossRef
Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery
Hossein Mahmoudvand, Farnaz Kheirandish, Ebrahim Saedi Dezaki, Saeedeh Shamsaddini, Majid Fasihi Harandi
Biomedicine & Pharmacotherapy.2016; 82: 393. CrossRef
Destructive effect of gamma irradiation on Echinococcus granulosus metacestodes
Yosra Hussein Alam-Eldin, Abeer Fathy Badawy
Parasitology Research.2015; 114(8): 3145. CrossRef
Evaluation of lethal effect of microwave exposure on protoscolices of hydatid cyst in vitro
Zahra Eslamirad, Homa Soleimani, Reza Hajihossein, Fatemeh Rafiei
Asian Pacific Journal of Tropical Disease.2015; 5(10): 821. CrossRef
Study of Protoscolicidal Effects of Hypertonic Glucose on Protoscolices of Hydatid Cyst at Different Concentrations and Exposure Times
Mojtaba Shahnazi, Fatemeh Badakhsh, Abbas Azadmehr, Mehrzad Saraei, Mahmood Alipour, Maryam Shahnazi, Mehri Jamshidi
International Scholarly Research Notices.2014; 2014: 1. CrossRef
Scolicidal Effects of Black Cumin Seed (Nigella sativa) Essential Oil on Hydatid Cysts
Hossein Mahmoudvand, Ebrahim Saedi Dezaki, Farnaz Kheirandish, Behrouz Ezatpour, Sareh Jahanbakhsh, Majid Fasihi Harandi
The Korean Journal of Parasitology.2014; 52(6): 653. CrossRef
Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts
Hossein Mahmoudvand, Majid Fasihi Harandi, Mojtaba Shakibaie, Mohammad Reza Aflatoonian, Naser ZiaAli, Mahsa Sadat Makki, Sareh Jahanbakhsh
International Journal of Surgery.2014; 12(5): 399. CrossRef
In vitro protoscolicidal effects of high-intensity focused ultrasound enhanced by a superabsorbent polymer
Jing Zhang, Bin Ye, Jing Kong, Hui Cai, Yifeng Zhao, Xiumin Han, Faqi Li
Parasitology Research.2013; 112(1): 385. CrossRef
Scolicidal Effects of Olea europaea and Satureja khuzestanica Extracts on Protoscolices of Hydatid Cysts
Mohammad Zibaei, Amanallah Sarlak, Bahram Delfan, Behrouz Ezatpour, Alireza Azargoon
The Korean Journal of Parasitology.2012; 50(1): 53. CrossRef
Echinococcus granulosus: Protoscolicidal effect of high intensity focused ultrasound
Xiaoyi Zou, Junan Wang, Hailong Zhao, Jing Zhang, Weihua Wu, Bin Ye
Experimental Parasitology.2009; 121(4): 312. CrossRef
Evaluation of Different Chemical Agents on the Germinative Layer of Sheep Hydatid Cyst After Implantation to Peritoneal Cavity of Balb/C
Soheil Mansourian, Seyed Mahmoud Sadjjadi, Seyed Vahid Hosseini, Zahra Barzin, Davood Mehrabani, Nader Tanideh, Ali Rafati
Journal of Investigative Surgery.2009; 22(3): 183. CrossRef
In Vitro Screening of DifferentAllium SativumExtracts on Hydatid Cysts Protoscoleces
Seyed Mahmoud Sadjjadi, Mohammad Reza Zoharizadeh, Mohammad Reza Panjeshahin
Journal of Investigative Surgery.2008; 21(6): 318. CrossRef
In Vitro Effectiveness of Different Chemical Agents on Scolices of Hydatid Cyst
Recep Caglar, Mehmet Fatih Yuzbasioglu, Ertan Bulbuloglu, Mustafa Gul, Fikret Ezberci, Ilhami Taner Kale
Journal of Investigative Surgery.2008; 21(2): 71. CrossRef