Parasites, Hosts and Diseases

"tachyzoite"

Original Articles

A New IgG Immunoblot Kit for Diagnosis of Toxoplasmosis in Pregnant Women: Imen Khammari, Fatma Saghrouni, Sami Lakhal, Aida Bouratbine, Moncef Ben Said, Jalel Boukadida; Korean J Parasitol 2014;52(5):493-499.
Published online October 22, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.5.493

Abstract
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The determination of the accurate immune status of pregnant women is crucial in order to prevent congenital toxoplasmosis. Equivocal results with conventional serological techniques are not uncommon when IgG titers are close to the cut-off value of the test, so that a confirmatory technique is needed. For this purpose, we developed a homemade immunoblot (IB) using soluble extract of Toxoplasma gondii tachyzoites and assessed it by testing 154 positive, 100 negative, and 123 equivocal sera obtained from pregnant women. In order to select the more valuable bands in terms of sensitivity and specificity, we used the Youden Index (YI). The highest YIs were those given by the 32, 36, 98, 21, and 33 bands. The simultaneous presence on the same blot of at least 3 bands showed a much higher YI (0.964) and was adapted as the positivity criterion. The analysis of results showed that our homemade IB correlated well with the commercial LDBIO Toxo II IgG® kit recently recommended as a confirmatory test (96.7% of concordance).

Citations

Citations to this article as recorded by

Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges
Priscila Silva Franco, Ana Carolina Morais Oliveira Scussel, Rafaela José Silva, Thadia Evelyn Araújo, Henrique Tomaz Gonzaga, Camila Ferreira Marcon, Joaquim Pedro Brito-de-Sousa, Angélica Lemos Debs Diniz, Marina Carvalho Paschoini, Bellisa Freitas Barb
Journal of Tropical Medicine.2024; 2024: 1. CrossRef
Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection
Min-ju Kim, Soeun J. Park, Hyunwoo Park
European Journal of Medical Research.2024;[Epub] CrossRef
Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study
Valeria Meroni, Francesca Genco, Luigia Scudeller, Marie-Pierre Brenier-Pinchart, Hélène Fricker-Hidalgo, Coralie L’Ollivier, Luc Paris, Hervé Pelloux
Pathogens.2022; 11(6): 665. CrossRef
Serological diagnosis of toxoplasmosis: evaluation of the commercial test recomLine Toxoplasma IgG immunoblot (Mikrogen) based on recombinant antigens
Vincent Jean-Pierre, Julien Miozzo, Hélène Fricker-Hidalgo, Cécile Garnaud, Marie Gladys Robert, Hervé Pelloux, Marie-Pierre Brenier-Pinchart
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The impact of lowering the cut-off value on the sensitivity of the Platelia Elisa IgG (Bio-Rad) test for toxoplasmosis diagnosis
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Real-Time RT-PCR on SAG1 and BAG1 Gene Expression during Stage Conversion in Immunosuppressed Mice Infected with Toxoplasma gondii Tehran Strain: Monavar Selseleh, Mohammad Hossein Modarressi, Mehdi Mohebali, Saeedeh Shojaee, Mohammad Reza Eshragian, Mina Selseleh, Ebrahim Azizi, Hossein Keshavarz; Korean J Parasitol 2012;50(3):199-205.
Published online August 13, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.3.199

Abstract
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Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

Citations

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