Parasites, Hosts and Diseases

"temperature"

Brief Communications

Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro: Parichart Boueroy, Kunyarat Duenngai, Chatanun Eamudomkarn, Panupan Sripan, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Atchara Artchayasawat, Jiraporn Songsri, Kanpicha Chomphumee, Panaratana Rattanasuwan, Porntip Laummaunwai, Sukhonthip Khueangchiangkhwang, Sirintip Boonjaraspinyo; Korean J Parasitol 2019;57(1):49-53.
Published online February 26, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.1.49

Abstract
PDF

Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage.

Citations

Citations to this article as recorded by

Development of a Dielectric Heating System for Selective Thermal Targeting of Liver Fluke Regions in Cirrhinus microlepis
Supatinee Kornsing, Sirigiet Phunklang, Chanchai Thongsopa, Piyaporn Krachodnok, Nuchanart Santalunai, Samran Santalunai
Applied Sciences.2025; 15(10): 5466. CrossRef
Viability of Trichinella spiralis in traditional sour pork fermentation and its inactivation by microwave heating: Implications for zoonotic risk and food safety
Atchara Artchayasawat, Benjamabhorn Pumhirunroj, Sukhonthip Khueangchiangkhwang, Thidarut Boonmars, Parichart Boueroy, Porntip Laummaunwai, Panaratana Rattanasuwan
Veterinary World.2025; : 1660. CrossRef

8,284 View
137 Download
5 Web of Science
Crossref

Quantitative Evaluation of Viability- and Apoptosis-Related Genes in Ascaris suum Eggs under Different Culture-Temperature Conditions: Yong-Man Yu, You-Hang Cho, Young-Nam Youn, Juan Hua Quan, In-Wook Choi, Young-Ha Lee; Korean J Parasitol 2012;50(3):243-247.
Published online August 13, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.3.243

Abstract
PDF

Ascaris suum eggs are inactivated by composting conditions; however, it is difficult to find functional changes in heat-treated A. suum eggs. Here, unembryonated A. suum eggs were incubated at 20℃, 50℃, and 70℃ in vitro, and the gene expression levels related to viability, such as eukaryotic translation initiation factor 4E (IF4E), phosphofructokinase 1 (PFK1), and thioredoxin 1 (TRX1), and to apoptosis, such as apoptosis-inducing factor 1 (AIF1) and cell death protein 6 (CDP6), were evaluated by real-time quantitative RT-PCR. No prominent morphological alterations were noted in the eggs at 20℃ until day 10. In contrast, the eggs developed rapidly, and embryonated eggs and hatched larvae began to die, starting on day 2 at 50℃ and day 1 at 70℃. At 20℃, IF4E, PFK1, and TRX1 mRNA expression was significantly increased from days 2-4; however, AIF1 and CDP6 mRNA expression was not changed significantly. IF4E, PFK1, and TRX1 mRNA expression was markedly decreased from day 2 at 50℃ and 70℃, whereas AIF1 and CDP6 mRNA expression was significantly increased. The expressions of HSP70 and HSP90 were detected for 9-10 days at 20℃, for 3-5 days at 50℃, and for 2 days at 70℃. Taken together, incremental heat increases were associated with the rapid development of A. suum eggs, decreased expression of genes related to viability, and earlier expression of apoptosis-related genes, and finally these changes of viability- and apoptosis-related genes of A. suum eggs were associated with survival of the eggs under temperature stress.

Citations

Citations to this article as recorded by

Environmental monitoring for Ascaris egg remediation: a critical review
Nathaniel I. Strong, Jack T. Andraka, Sung-Geun Woo, Sahar H. El Abbadi, Aaron J. Lewis, Sindy K. Y. Tang, Stephen P. Luby, Craig S. Criddle
Environmental Science and Pollution Research.2025; 32(17): 10688. CrossRef
Investigating cement-based surfaces as a sustainable flooring solution to improve Ascaris Suum egg removal and inactivation in low-resource settings
Claire E. Anderson, Suhi Hanif, Jason Hernandez, Yoshika Crider, Michael Lepech, Sarah L. Billington, Alexandria B. Boehm, Jade Benjamin-Chung, Vito Colella,
PLOS Neglected Tropical Diseases.2025; 19(10): e0012919. CrossRef
Chancen und Risiken der Nutzung genetischer Resistenzen gegen Infektionskrankheiten beim Schwein – eine Übersicht
Doris Höltig, Gerald Reiner
Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere.2022; 50(01): 46. CrossRef
Expression patterns and structural modelling of Hsp70 and Hsp90 in a fish-borne zoonotic nematode Anisakis pegreffii
Hui-Yu Chen, Yi-Sheng Cheng, Hsiu-Hui Shih
Veterinary Parasitology.2015; 212(3-4): 281. CrossRef
Effects of Some Pesticides on Development of Ascaris suum Eggs
Yong-Man Yu, Jin-Won Kim, Won-Seok Na, Young-Nam Youn, In-Wook Choi, Young-Ha Lee
The Korean Journal of Parasitology.2014; 52(1): 111. CrossRef

9,222 View
69 Download
Crossref

Effect of Temperature on Embryonation of Ascaris suum Eggs in an Environmental Chamber: Min-Ki Kim, Kyoung-Ho Pyo, Young-Sang Hwang, Ki Hwan Park, In Gyun Hwang, Jong-Yil Chai, Eun-Hee Shin; Korean J Parasitol 2012;50(3):239-242.
Published online August 13, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.3.239

Abstract
PDF

The influence of temperature on the development and embryonation of Ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. The time required for development and embryonation of eggs was examined under 3 different temperature conditions, 5℃, 25℃, and 35℃. A. suum eggs did not develop over 1 month at the temperature of 5℃. However, other temperature conditions, 25℃ and 35℃, induced egg development to the 8-cell-stage at days 5-6 after incubation. All eggs examined developed to the 8-cell stage at day 6 after incubation in the sand medium at 25℃. The higher temperature, 35℃, slightly accelerated the A. suum egg development compared to 25℃, and the development to the 8-cell stage occurred within day 5 after incubation. The formation of larvae in A. suum eggs at temperatures of 35℃ and 25℃ appeared at days 17 and 19 after incubation, respectively. These findings show that 35℃ condition shortens the time for the development of A. suum eggs to the 8-cell-stage in comparison to 25℃, and suggest the possibility of accelerated transmission of this parasite, resulting from global warming and ecosystem changes.

Citations

Citations to this article as recorded by

Possibilities of decontaminating organic waste from swine-farming complexes using anaerobic digestion
Olexandra Boyko, Viktor Brygadyrenko, Yelizaveta Chernysh, Viktoriia Chubur, Hynek Roubík
Biomass Conversion and Biorefinery.2025; 15(7): 11175. CrossRef
Report of the Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) on the effects of climate change on the risk of transmission of foodborne pathogens
Antonio Valero Díaz, Rosa María Capita González, Baltasar Mayo Pérez, Azucena del Carmen Mora Gutiérrez, María Dolores Rodrigo Aliaga, Gloria Sánchez Moragas, Paula Arrabal Durán
Food Risk Assess Europe.2025;[Epub] CrossRef
Spatial modeling of soil-transmitted helminthiases in Colombia under climate change scenarios
Mario J. Olivera, Julián Felipe Porras-Villamil, Màrius Vicent Fuentes
Biomédica.2025; 45(Sp. 2): 68. CrossRef
Exposure of American Black Bears (Ursus americanus) to Ticks, Tick-Borne Diseases, and Intestinal Parasites in Wisconsin
Nika S. Reichert, Daniela Mathieu, Christopher J. Katz, Kent A. Hatch
Diversity.2024; 16(9): 537. CrossRef
Environmental modulators on the development of the raccoon roundworm (Baylisascaris procyonis): Effects of temperature on the embryogenesis
Robin Stutz, Dorian D. Dörge, Anna V. Schantz, Norbert Peter, Sven Klimpel
International Journal for Parasitology: Parasites and Wildlife.2024; 25: 100997. CrossRef
Influence of Tempetature on Viability and Deveplopment of Heterakis gallinarum (Nematoda, Heterakidae) Eggs
V. Yevstafieva, O. Omelchenko , V. Melnychuk , L. Nagorna , M. Petrenko , B. Shaferivskyi , S. Kravchenko , K. Suprunenko , L. Karysheva , S. Sheiko , T. Burda , N. Syzonenko , O. Savenkova , V. Liulka
zoodiversity.2024; 58(5): 435. CrossRef
Analysis of application status and development prospect of environmental chambers: A review
Dong Xie, Haixia Zhou, Lifeng Zhou, Chuck Wah Yu
Indoor and Built Environment.2023; 32(2): 305. CrossRef
Why Are We Still a Worm World in the 2020s? An Overview of Risk Factors and Endemicity for Soil-Transmitted Helminthiasis
Adelson Ferreira Ramos Neto, Yvanna Louise Di Christine Oliveira, Luciana Maria de Oliveira, Roseli La Corte, Sona Jain, Divaldo Pereira de Lyra Junior, Ricardo Toshio Fujiwara, Silvio Santana Dolabella
Acta Parasitologica.2023; 68(3): 481. CrossRef
Epidemiology of gastrointestinal helminths among chickens (Gallus domesticus) from Borno State, Northeastern Nigeria: prevalence, helminth burden and associated risk factors
Jallailudeen Rabana Lawal, Saleh Mohammed Jajere, Umar Isa Ibrahim, Abdullahi Abubakar Biu, Dauda Jonathan
Revue d’élevage et de médecine vétérinaire des pays tropicaux.2023; 76: 1. CrossRef
The Prospective Effects of Climate Change on Neglected Tropical Diseases in the Eastern Mediterranean Region: a Review
Ahmed K. Al-Delaimy
Current Environmental Health Reports.2022; 9(2): 315. CrossRef
Method optimisation for prolonged laboratory storage of Ascaridia galli eggs
Anwar Shifaw, Teka Feyera, Timothy Elliott, Brendan Sharpe, Isabelle Ruhnke, Stephen W. Walkden-Brown
Veterinary Parasitology.2022; 309: 109758. CrossRef
Schistosoma mansoni and soil-transmitted helminths among schoolchildren in An-Nadirah District, Ibb Governorate, Yemen after a decade of preventive chemotherapy
Walid M. S. Al-Murisi, Abdulsalam M. Al-Mekhlafi, Mohammed A. K. Mahdy, Sami Ahmed Al-Haidari, Dhekra A. Annuzaili, Ahmed Ali Qaid Thabit, Marcello Otake Sato
PLOS ONE.2022; 17(8): e0273503. CrossRef
Ascaridia galli eggs obtained from fresh excreta, worm uteri or worms cultured in artificial media differ in embryonation capacity and infectivity
Anwar Shifaw, Isabelle Ruhnke, Timothy Elliott, Brendan Sharpe, Teka Feyera, Stephen W. Walkden-Brown
Veterinary Parasitology.2022; 310: 109792. CrossRef
The Effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L., Satureja hortensis L. and Calendula officinalis L. on the Embryogenesis of Ascaris suum Eggs during an In Vitro Experimental Study
Mihai-Horia Băieş, Călin Gherman, Zsolt Boros, Diana Olah, Ana-Maria Vlase, Anamaria Cozma-Petruț, Adriana Györke, Doina Miere, Laurian Vlase, Gianina Crișan, Marina Spînu, Vasile Cozma
Pathogens.2022; 11(9): 1065. CrossRef
Persistent Ascaris Transmission Is Possible in Urban Areas Even Where Sanitation Coverage Is High
Drew Capone, Troy Barker, Oliver Cumming, Abeoseh Flemister, Riley Geason, Elizabeth Kim, Jackie Knee, Yarrow Linden, Musa Manga, Mackenzie Meldrum, Rassul Nala, Simrill Smith, Joe Brown
Environmental Science & Technology.2022; 56(22): 15969. CrossRef
The impact of climate change on neglected tropical diseases: a systematic review
Rachel Tidman, Bernadette Abela-Ridder, Rafael Ruiz de Castañeda
Transactions of The Royal Society of Tropical Medicine and Hygiene.2021; 115(2): 147. CrossRef
Effective low-cost preservation of human stools in field-based studies for helminth and microbiota analysis
Katharina Stracke, Poom Adisakwattana, Suparat Phuanukoonnon, Tippayarat Yoonuan, Akkarin Poodeepiyasawat, Paron Dekumyoy, Kittipong Chaisiri, Alexandra Roth Schulze, Stephen Wilcox, Harin Karunajeewa, Rebecca J. Traub, Aaron R. Jex
International Journal for Parasitology.2021; 51(9): 741. CrossRef
Ascaris lumbricoideseggs or artefacts? A diagnostic conundrum
M. P. Maurelli, L. C. Alves, C. S. Aggarwal, P. Cociancic, B. Levecke, P. Cools, A. Montresor, D. Ianniello, L. Gualdieri, G. Cringoli, L. Rinaldi
Parasitology.2021; 148(13): 1554. CrossRef
Inactivating Effects of Common Laboratory Disinfectants, Fixatives, and Temperatures on the Eggs of Soil Transmitted Helminths
Kristine J. Kines, Mark Fox, MacKevin Ndubuisi, Guilherme G. Verocai, Vitaliano Cama, Richard S. Bradbury, Anat Florentin
Microbiology Spectrum.2021;[Epub] CrossRef
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh
Jade Benjamin-Chung, Nils Pilotte, Ayse Ercumen, Jessica R. Grant, Jacqueline R. M. A. Maasch, Andrew M. Gonzalez, Ashanta C. Ester, Benjamin F. Arnold, Mahbubur Rahman, Rashidul Haque, Alan E. Hubbard, Stephen P. Luby, Steven A. Williams, John M. Colford
PLOS Neglected Tropical Diseases.2020; 14(4): e0008087. CrossRef
Viability and development of Ascaridia galli eggs recovered in artificial media followed by storage under different conditions
T. Feyera, I. Ruhnke, B. Sharpe, T. Elliott, D.L.M. Campbell, S.W. Walkden-Brown
Journal of Helminthology.2020;[Epub] CrossRef
Molecular identification of potential intermediate hosts of Aulonocephalus pennula from the order Orthoptera
C. Henry, M.Z. Brym, A. Kalyanasundaram, R.J. Kendall
Journal of Helminthology.2019; 93(2): 166. CrossRef
Quick incubation process to determine inactivation of Ascaris and Toxocara eggs
C. Maya, M. Pérez, G. Velásquez, J. A. Barrios, A. Román, B. Jiménez
Water Science and Technology.2019; 80(12): 2328. CrossRef
Manure management and public health: Sanitary and socio-economic aspects among urban livestock-keepers in Cambodia
Gunilla Ström, Ann Albihn, Tomas Jinnerot, Sofia Boqvist, Agnes Andersson-Djurfeldt, Seng Sokerya, Kristina Osbjer, Sorn San, Holl Davun, Ulf Magnusson
Science of The Total Environment.2018; 621: 193. CrossRef
Seasonally timed treatment programs for Ascaris lumbricoides to increase impact—An investigation using mathematical models
Emma L. Davis, Leon Danon, Joaquín M. Prada, Sharmini A. Gunawardena, James E. Truscott, Johnny Vlaminck, Roy M. Anderson, Bruno Levecke, Eric R Morgan, T. Deirdre Hollingsworth, Guillermina Avila
PLOS Neglected Tropical Diseases.2018; 12(1): e0006195. CrossRef
The impact of seasonality on the dynamics and control of Ascaris lumbricoides infections
A.J. Cooper, T. Déirdre Hollingsworth
Journal of Theoretical Biology.2018; 453: 96. CrossRef
The time–temperature relationship for the inactivation of Ascaris eggs
D. Naidoo, G. L. Foutch
Journal of Water, Sanitation and Hygiene for Development.2018; 8(1): 123. CrossRef
Prevalence of Toxoplasma gondii and Potentially Zoonotic Helminths in Wild Boars (Sus scrofa) Hunted in Central Italy
Roberto Amerigo Papini, Sara Vannucci, Guido Rocchigiani, Simona Nardoni, Francesca Mancianti
Macedonian Veterinary Review.2018; 41(1): 83. CrossRef
Seroprevalence of five parasitic pathogens in pregnant women in ten Caribbean countries
Fengguang Guo, Martin S. Forde, Stephen R. Werre, Rosina C. Krecek, Guan Zhu
Parasitology Research.2017; 116(1): 347. CrossRef
Effects of climate change on the occurrence and distribution of livestock diseases
B. Bett, P. Kiunga, J. Gachohi, C. Sindato, D. Mbotha, T. Robinson, J. Lindahl, D. Grace
Preventive Veterinary Medicine.2017; 137: 119. CrossRef
A multilevel study of the environmental determinants of swine ascariasis in England
Ângelo Joel Mendes, Ana Isabel Ribeiro, Milton Severo, João Niza-Ribeiro
Preventive Veterinary Medicine.2017; 148: 10. CrossRef
Climate Change Contribution to the Emergence or Re-Emergence of Parasitic Diseases
Erica E Short, Cyril Caminade, Bolaji N Thomas
Infectious Diseases: Research and Treatment.2017;[Epub] CrossRef
Embryonation ability of Ascaridia galli eggs isolated from worm uteri or host faeces
Shayan Rahimian, Matthias Gauly, Gürbüz Daş
Veterinary Parasitology.2016; 215: 29. CrossRef
Temperature dependent embryonic development of Trichuris suis eggs in a medicinal raw material
Nermina Vejzagić, Helene Kringel, Johan Musaeus Bruun, Allan Roepstorff, Stig Milan Thamsborg, Anette Blak Grossi, Christian M.O. Kapel
Veterinary Parasitology.2016; 215: 48. CrossRef
Climate change and foodborne transmission of parasites: A consideration of possible interactions and impacts for selected parasites
Kjersti Selstad Utaaker, Lucy J. Robertson
Food Research International.2015; 68: 16. CrossRef
Effect of temperature and shear stress on the viability of Ascaris suum
J. E. Thomas, J. T. Podichetty, Y. Shi, D. Belcher, R. Dunlap, K. McNamara, M. V. Reichard, J. Smay, A. J. Johannes, G. L. Foutch
Journal of Water, Sanitation and Hygiene for Development.2015; 5(3): 402. CrossRef
Effect of fenbendazole on shedding and embryonation of Ascaris suum eggs from naturally infected sows
Jeremy Pittman, Gil Myers, Kenneth Stalder, Locke Karriker
Journal of Swine Health and Production.2015; 23(5): 252. CrossRef
Effects of Some Pesticides on Development of Ascaris suum Eggs
Yong-Man Yu, Jin-Won Kim, Won-Seok Na, Young-Nam Youn, In-Wook Choi, Young-Ha Lee
The Korean Journal of Parasitology.2014; 52(1): 111. CrossRef
Assessment of parasitic contamination of raw vegetables in Mannuthy, Kerala state, India
B. Sunil, Divya Rani Thomas, C. Latha, H. Shameem
Veterinary World.2014; 7(4): 253. CrossRef

15,530 View
195 Download
Crossref

Effects of Kimchi Extract and Temperature on Embryostasis of Ascaris suum Eggs: Jin-Sung Kim, Dae-Sung Oh, Kyu-Sung Ahn, Sung-Shik Shin; Korean J Parasitol 2012;50(1):83-87.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.83

Abstract
PDF

To determine the effects of kimchi extracts at different temperatures on larval development, Ascaris suum eggs were mixed with soluble part of 7 different brands of commercially available kimchi and preserved at either 5℃ or 25℃ for up to 60 days. A. suum eggs incubated at 25℃ showed marked differences in larval development between kimchi extract and control group. While all eggs in the control group completed embryonation by day 21, only 30% of the eggs in the kimchi extract group became embryonated by day 36 and about 25% never became larvated even at day 60. At 5℃, however, none of the eggs showed larval development regardless of the incubation period or type of mixture group. To determine the survival rate of A. suum eggs that showed no embryonation after being preserved at 5℃, eggs preserved in kimchi extracts for 14, 28, and 60 at 5℃ were re-incubated at 25℃ for 3 weeks in distilled water. While all eggs in the control group became larvated, eggs in the kimchi extract group showed differences in their embryonation rates by the incubation period; 87.4 % and 41.7% of the eggs became embryonated after being refrigerated for 14 days and 28 days, respectively. When refrigerated for 60 days, however, no eggs mixed in kimchi extract showed larval development. Our results indicate that embryogenesis of A. suum eggs in kimchi extract was affected by duration of refrigeration, and that all eggs stopped larval development completely in kimchi kept at 5℃ for up to 60 days.

Citations

Citations to this article as recorded by

Reconciling Egg- and Antigen-Based Estimates of Schistosoma mansoni Clearance and Reinfection: A Modeling Study
Jessica Clark, Arinaitwe Moses, Andrina Nankasi, Christina L Faust, Adriko Moses, Diana Ajambo, Fred Besigye, Aaron Atuhaire, Aidah Wamboko, Lauren V Carruthers, Rachel Francoeur, Edridah M Tukahebwa, Joaquin M Prada, Poppy H L Lamberton
Clinical Infectious Diseases.2022; 74(9): 1557. CrossRef
2-Methyl-pentanoyl-carnitine (2-MPC): a urine biomarker for patent Ascaris lumbricoides infection
Ole Lagatie, Ann Verheyen, Stijn Van Asten, Maurice R. Odiere, Yenny Djuardi, Bruno Levecke, Johnny Vlaminck, Zeleke Mekonnen, Daniel Dana, Ruben T’Kindt, Koen Sandra, Rianne van Outersterp, Jos Oomens, Ronghui Lin, Lieve Dillen, Rob Vreeken, Filip Cuycke
Scientific Reports.2020;[Epub] CrossRef
Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA
Ole Lagatie, Ann Verheyen, Kim Van Hoof, Dax Lauwers, Maurice R. Odiere, Johnny Vlaminck, Bruno Levecke, Lieven J. Stuyver, Timothy G. Geary
PLOS Neglected Tropical Diseases.2020; 14(10): e0008807. CrossRef
Praziquantel decreases fecundity in Schistosoma mansoni adult worms that survive treatment: evidence from a laboratory life-history trade-offs selection study
Poppy H.L. Lamberton, Christina L. Faust, Joanne P. Webster
Infectious Diseases of Poverty.2017;[Epub] CrossRef
The capability of the fungus Mucor circinelloides to maintain parasiticidal activity after the industrial feed pelleting enhances the possibilities of biological control of livestock parasites
F.L. Arroyo, M.S. Arias, C.F. Cazapal-Monteiro, J.A. Hernández, J. Suárez, S. Miguélez, A. Romasanta, R. Sánchez-Andrade, A. Paz-Silva
Biological Control.2016; 92: 38. CrossRef
Effects of Some Pesticides on Development of Ascaris suum Eggs
Yong-Man Yu, Jin-Won Kim, Won-Seok Na, Young-Nam Youn, In-Wook Choi, Young-Ha Lee
The Korean Journal of Parasitology.2014; 52(1): 111. CrossRef
Sensitivity and Specificity of Multiple Kato-Katz Thick Smears and a Circulating Cathodic Antigen Test for Schistosoma mansoni Diagnosis Pre- and Post-repeated-Praziquantel Treatment
Poppy H. L. Lamberton, Narcis B. Kabatereine, David W. Oguttu, Alan Fenwick, Joanne P. Webster, Stefanie Knopp
PLoS Neglected Tropical Diseases.2014; 8(9): e3139. CrossRef
Inactivation of Ascaris suum eggs
Somsri Wiwanitkit, Viroj Wiwanitkit
American Journal of Infection Control.2013; 41(9): 849. CrossRef
Effect of Temperature on Embryonation of Ascaris suum Eggs in an Environmental Chamber
Min-Ki Kim, Kyoung-Ho Pyo, Young-Sang Hwang, Ki Hwan Park, In Gyun Hwang, Jong-Yil Chai, Eun-Hee Shin
The Korean Journal of Parasitology.2012; 50(3): 239. CrossRef

9,784 View
59 Download
Crossref

Original Article

Detection and characterization of excretory/secretory proteins from Toxoplasma gondii by monoclonal antibodies: Eui-Sun Son, Ho-Woo Nam; Korean J Parasitol 2001;39(1):49-56.
Published online March 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.1.49

Abstract
PDF

Excretory/secretory proteins (ESP) from Toxoplasma gondii were analyzed to define the function in the penetration process into host cells. Whole ESP obtained at 37℃ were composed of 15 bands with molecular mass of 110, 97, 86, 80, 70, 60, 54, 42, 40, 36, 30, 28, 26, 22, and 19 kDa. Five ESP of 86, 80, 42, 36, and 28 kDa were reacted with monoclonal antibodies (mAb), named as Tg386 (microneme), Tg485 (surface membrane), Tg786 (rhoptry), Tg378, and Tg556 (both dense granules), respectively. The ESP was released by a temperature-dependent/-independent manner and all at once whenever ready to pour out except Tg786. Each ESP was not exhausted within the parasite but the amount was limited. Tg786 was released continuously with increment, whereas Tg378 and Tg556 were ceased to release after 3 and 4 hr. Dense granular Tg378 and Tg556 were released spontaneously and constitutively before the entry into host cells also. The entry of T. gondii was inhibited by all the mAbs differentially. And the parasite deprived of ESP was inhibited to enter exponentially up to 90.1%. It is suggested that ESP play an essential function to provide appropriate environment for the entry of the parasite into host cells.

Citations

Citations to this article as recorded by

T. gondii excretory proteins promote the osteogenic differentiation of human bone mesenchymal stem cells via the BMP/Smad signaling pathway
Mingzhu Deng, Feifei Gao, Tianfeng Liu, Weiqiang Zhan, Juanhua Quan, Ziquan Zhao, Xuyang Wu, Zhuolan Zhong, Hong Zheng, Jiaqi Chu
Journal of Orthopaedic Surgery and Research.2024;[Epub] CrossRef
FAF1 downregulation by Toxoplasma gondii enables host IRF3 mobilization and promotes parasite growth
Fei‐Fei Gao, Juan‐Hua Quan, In‐Wook Choi, Yeon‐Jae Lee, Seul‐Gi Jang, Jae‐Min Yuk, Young‐Ha Lee, Guang‐Ho Cha
Journal of Cellular and Molecular Medicine.2021; 25(19): 9460. CrossRef
Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line
Hao Wang, Chunchao Li, Wei Ye, Zhaobin Pan, Jinhui Sun, Mingzhu Deng, Weiqiang Zhan, Jiaqi Chu
The Korean Journal of Parasitology.2021; 59(6): 573. CrossRef
FOXO1-Mediated NOX4 Expression is Required for Host to Defense in the Model of Ocular Toxoplasmosis
Wei Zhou, Fei-Fei Gao, Jang Ho Lee, Im-Soon Lee, Jae-Min Yuk, Kyoung Sang Cho, Young-Ha Lee, Guang-Ho Cha
SSRN Electronic Journal .2021;[Epub] CrossRef
The Role of PI3K/AKT Pathway and NADPH Oxidase 4 in Host ROS Manipulation by Toxoplasma gondii
Hei Gwon Choi, Fei-Fei Gao, Wei Zhou, Pu-Reum Sun, Jae-Min Yuk, Young-Ha Lee, Guang-Ho Cha
The Korean Journal of Parasitology.2020; 58(3): 237. CrossRef
Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for diagnosis of toxoplasmosis by ELISA test
S. Pishkari, S. Shojaee, H. Keshavarz, M. Salimi, M. Mohebali
Journal of Parasitic Diseases.2017; 41(1): 289. CrossRef
A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18
Zhaoshou Yang, Yongheng Hou, Taofang Hao, Hee-Sool Rho, Jun Wan, Yizhao Luan, Xin Gao, Jianping Yao, Aihua Pan, Zhi Xie, Jiang Qian, Wanqin Liao, Heng Zhu, Xingwang Zhou
Molecular & Cellular Proteomics.2017; 16(3): 469. CrossRef
Investigation of Toxoplasma gondii Infection in Cutaneous Leishmaniasis Patients of the Isfahan Province
Abbasali Eskandarian, Somayeh Jahani, Hossein Hejazi, Hossein Yousefi, Vahid Raissi
International Journal of Infection.2016;[Epub] CrossRef
Investigation of Toxoplasma gondii Infection in Cutaneous Leishmaniasis Patients of the Isfahan Province
Abbasali Eskandarian, Somayeh Jahani, Hossein Hejazi, Hossein Yousefi, Vahid Raissi
International Journal of Infection.2016;[Epub] CrossRef
Electrophoretic Patterns of Toxoplasma gondii Excreted/Secreted Antigens and Their Role in Induction of the Humoral Immune Response
Ahmad Daryani, Mehdi Sharif, Hamed Kalani, Alireza Rafiei, Farzad Kalani, Ehsan Ahmadpour
Jundishapur Journal of Microbiology.2014;[Epub] CrossRef
Rhoptry protein 6 from Toxoplasma gondii is an intrinsically disordered protein
Won-Kyu Lee, Hye-Jin Ahn, Yeon Gyu Yu, Ho-Woo Nam
Protein Expression and Purification.2014; 101: 146. CrossRef
Comprehensive Proteome Analysis of the Excretory/Secretory Proteins of Toxoplasma gondii
Won-Kyu Lee, Hye-Jin Ahn, Je-Hyun Baek, Chong-Heon Lee, Yeon Gyu Yu, Ho-Woo Nam
Bulletin of the Korean Chemical Society.2014; 35(10): 3071. CrossRef
High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles
Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam
The Korean Journal of Parasitology.2014; 52(4): 367. CrossRef
Heat Shock Protein 90 of Bonamia ostreae: Characterization and Possible Correlation with Infection of the Flat Oyster, Ostrea edulis
Maria Prado‐Alvarez, Bruno Chollet, Yann Couraleau, Benjamin Morga, Isabelle Arzul
Journal of Eukaryotic Microbiology.2013; 60(3): 257. CrossRef
Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells
Juan-Hua Quan, Wei Zhou, Guang-Ho Cha, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2013; 51(1): 85. CrossRef
Toxoplasma gondii Proliferation Require Down-Regulation of Host Nox4 Expression via Activation of PI3 Kinase/Akt Signaling Pathway
Wei Zhou, Juan-Hua Quan, Young-Ha Lee, Dae-Whan Shin, Guang-Ho Cha, Ira J. Blader
PLoS ONE.2013; 8(6): e66306. CrossRef
Evaluation of Excreted/Secreted Antigens Derived from Peritoneal of Toxoplasma Infected Small Mice to Detect IgG Against Toxoplasma
Seyed Hossein Abdollahi, Mohammad Kazemi Arababadi, Gholamhossein Hassanshah
Pakistan Journal of Biological Sciences.2009; 12(6): 530. CrossRef
Interaction between Parasitophorous Vacuolar Membrane-associated GRA3 and Calcium Modulating Ligand of Host Cell Endoplasmic Reticulum in the Parasitism of Toxoplasma gondii
Ji Yeon Kim, Hye-Jin Ahn, Kyung Ju Ryu, Ho-Woo Nam
The Korean Journal of Parasitology.2008; 46(4): 209. CrossRef
Host cell binding of GRA10, a novel, constitutively secreted dense granular protein from Toxoplasma gondii
Hye-Jin Ahn, Sehra Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2005; 331(2): 614. CrossRef
USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES
Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, Jong-Yil Chai
Journal of Parasitology.2004; 90(1): 161. CrossRef
Protease activity of 80 kDa protein secreted from the apicomplexan parasite Toxoplasma gondii
Kyoung-Ju Song, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(3): 165. CrossRef
Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients
Hye-Jin Ahn, Sera Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(2): 89. CrossRef
Molecular cloning of the 82-kDa heat shock protein (HSP90) of Toxoplasma gondii associated with the entry into and growth in host cells
Hye-Jin Ahn, Sera Kim, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2003; 311(3): 654. CrossRef
Protease Activity and Host Cell Binding of the 42-kDa Rhoptry Protein from Toxoplasma gondii after Secretion
Hye-Jin Ahn, Kyoung-Ju Song, Eui-Sun Son, Jong-Chul Shin, Ho-Woo Nam
Biochemical and Biophysical Research Communications.2001; 287(3): 630. CrossRef
Molecular cloning and characterization of peroxiredoxin from Toxoplasma gondii
Eui-Sun Son, Kyoung-Ju Song, Jong-Chul Shin, Ho-Woo Nam
The Korean Journal of Parasitology.2001; 39(2): 133. CrossRef
Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo
Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee
The Korean Journal of Parasitology.2001; 39(3): 233. CrossRef