Parasites, Hosts and Diseases

"vaccination"

Original Articles

Protective and Anti-Pathology Effects of Sm Fructose-1,6-Bisphosphate Aldolase-Based DNA Vaccine against Schistosoma mansoni by Changing Route of Injection: Mohamed Saber, Tarek Diab, Olft Hammam, Amr Karim, Amina Medhat, Mamdouh Khela, Ehab El-Dabaa; Korean J Parasitol 2013;51(2):155-163.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.155

Abstract
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This study aimed to evaluate the efficacy of fructose-1,6-bis phosphate aldolase (SMALDO) DNA vaccination against Schistosoma mansoni infection using different routes of injection. The SMALDO has been cloned into the eukaryotic expression vector pcDNA3.1/V5-His TOPO-TA and was used in injecting Swiss albino mice intramuscularly (IM), subcutaneously (SC), or intraperitoneally (IP) (50 ?g/mouse). Mice vaccinated with non-recombinant pcDNA3.1 served as controls. Each group was immunized 4 times at weeks 0, 2, 4, and 6. Two weeks after the last booster dose, all mice groups were infected with 80 S. mansoni cercariae via tail immersion. At week 8 post-infection, animals were sacrificed for assessment of parasitological and histopathological parameters. High anti-SMALDO IgG antibody titers were detected in sera of all vaccinated groups (P<0.01) compared to the control group. Both the IP and SC vaccination routes resulted in a significant reduction in worm burden (46.2% and 28.9%, respectively, P<0.01). This was accompanied by a significant reduction in hepatic and intestinal egg counts (41.7% and 40.2%, respectively, P<0.01) in the IP group only. The number of dead eggs was significantly increased in both IP and IM groups (P<0.01). IP vaccination recorded the highest significant reduction in granuloma number and diameter (54.7% and 29.2%, respectively, P<0.01) and significant increase in dead miracidia (P<0.01). In conclusion, changing the injection route of SMALDO DNA vaccination significantly influenced the efficacy of vaccination. SMALDO DNA vaccination via IP route could be a promising protective and anti-pathology vaccine candidate against S. mansoni infection.

Citations

Citations to this article as recorded by

Glycolytic Enzymes as Vaccines Against Schistosomiasis: Testing Schistosoma mansoni Phosphoglycerate Mutase in Mice
David B. Pirovich, Akram A. Da'dara, Patrick J. Skelly
Journal of Parasitology.2024;[Epub] CrossRef
Multifunctional Fructose 1,6-Bisphosphate Aldolase as a Therapeutic Target
David B. Pirovich, Akram A. Da’dara, Patrick J. Skelly
Frontiers in Molecular Biosciences.2021;[Epub] CrossRef
Characterization of Fructose-1,6-Bisphosphate Aldolase 1 of Echinococcus multilocularis
Xuedong He, Jing Zhang, Yue Sun, Tianyan Lan, Xiaola Guo, Xiaoqiang Wang, Omnia M. Kandil, Mazhar Ayaz, Xuenong Luo, Houhui Song, Yadong Zheng
Veterinary Sciences.2021; 9(1): 4. CrossRef
Proteomic analysis of adult Schistosoma mekongi somatic and excretory-secretory proteins
Onrapak Reamtong, Nattapon Simanon, Tipparat Thiangtrongjit, Yanin Limpanont, Phiraphol Chusongsang, Yupa Chusongsang, Songtham Anuntakarun, Sunchai Payungporn, Orawan Phuphisut, Poom Adisakwattana
Acta Tropica.2020; 202: 105247. CrossRef
Amelioration of type 1 diabetes by recombinant fructose-1,6-bisphosphate aldolase and cystatin derived from Schistosoma japonicum in a murine model
Ke Yan, Bo Wang, Huabang Zhou, Qingli Luo, Jilong Shen, Yunxia Xu, Zhengrong Zhong
Parasitology Research.2020; 119(1): 203. CrossRef
Linking murine resistance to secondary cystic echinococcosis with antibody responses targeting Echinococcus granulosus tegumental antigens
Sebastián Miles, Javier Magnone, Marek Cyrklaff, Paula Arbildi, Friedrich Frischknecht, Sylvia Dematteis, Gustavo Mourglia-Ettlin
Immunobiology.2020; 225(3): 151916. CrossRef
Interactome analysis of CD5 and CD6 ectodomains with tegumental antigens from the helminth parasite Echinococcus granulosus sensu lato
Sebastián Miles, María Velasco-de-Andrés, Francisco Lozano, Gustavo Mourglia-Ettlin
International Journal of Biological Macromolecules.2020; 164: 3718. CrossRef
Protein extract from head-foot tissue of Oncomelania hupensis promotes the growth and development of mother sporocysts of Schistosoma japonicum via upregulation of parasite aldolase gene
Ting Chai, Sijing Zhu, Huifen Dong, Zhenping Ming
Parasitology Research.2019; 118(6): 1821. CrossRef
Comparative study of excretory–secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata
Conor E. Fogarty, Min Zhao, Donald P. McManus, Mary G. Duke, Scott F. Cummins, Tianfang Wang
Parasites & Vectors.2019;[Epub] CrossRef
Why Do Intravascular Schistosomes Coat Themselves in Glycolytic Enzymes?
David Pirovich, Akram A. Da'dara, Patrick J. Skelly
BioEssays.2019;[Epub] CrossRef
Schistosomiasis in Egypt: A never-ending story?
Ahmad A. Othman, Rasha H. Soliman
Acta Tropica.2015; 148: 179. CrossRef
Cloning, expression, and partial characterization of FBPA from Schistosoma japonicum, a molecule on that the fluke may develop nutrition competition and immune evasion from human
Qiping Hu, Huiqiong Xie, Shuyu Zhu, Dejun Liao, Tingzheng Zhan, Dengyu Liu
Parasitology Research.2015; 114(9): 3459. CrossRef
Evaluation of Echinostoma liei worm, metacercaria and redia antigens for schistosomiasis control
G. Abdel-Monaem, A. Farid, I. Rabia, A. El-Amir
Experimental Parasitology.2015; 157: 23. CrossRef

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Protective Role of Purified Cysteine Proteinases against Fasciola gigantica Infection in Experimental Animals: Eman EL-Ahwany, Ibrahim Rabia, Faten Nagy, Mona Zoheiry, Tarek Diab, Suher Zada; Korean J Parasitol 2012;50(1):45-51.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.45

Abstract
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Fascioliasis is one of the public health problems in the world. Cysteine proteinases (CP) released by Fasciola gigantica play a key role in parasite feeding, migration through host tissues, and in immune evasion. There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infections. Cysteine proteinases were purified and tested in vaccine trials of sheep infected with the liver fluke. Multiple doses (2 mg of CP in Freund's adjuvant followed by 3 booster doses 1 mg each at 4 week intervals) were injected intramuscularly into sheep 1 week prior to infect orally with 300 F. gigantica metacercariae. All the sheep were humanely slaughtered 12 weeks after the first immunization. Changes in the worm burden, ova count, and humoral and cellular responses were evaluated. Significant reduction was observed in the worm burden (56.9%), bile egg count (70.7%), and fecel egg count (75.2%). Immunization with CP was also found to be associated with increases of total IgG, IgG₁, and IgG₂ (P<0.05). Data showed that the serum cytokine levels of pro-inflammatory cytokines, IL-12, IFN-γ, and TNF-α, revealed significant decreases (P<0.05). However, the anti-inflammatory cytokine levels, IL-10, TGF-β, and IL-6, showed significant increases (P<0.05). In conclusion, it has been found that CP released by F. gigantica are highly important candidates for a vaccine antigen because of their role in the fluke biology and host-parasite relationships.

Citations

Citations to this article as recorded by

Genetic diversity and adaptability of native sheep breeds from different climatic zones
George Wanjala, Zoltán Bagi, Dinu Gavojdian, Bouabid Badaoui, Putri Kusuma Astuti, Alexandru Mizeranschi, Elena Ilisiu, Husein Ohran, Eva Pasic Juhas, Dimitrios Loukovitis, Aldona Kawęcka, Rūta Šveistienė, Zsolt Becskei, Péter Strausz, Nelly Kichamu, Szil
Scientific Reports.2025;[Epub] CrossRef
Serodiagnosis of Fasciola gigantica Infection in Buffaloes with Native Cathepsin-L Proteases and Recombinant Cathepsin L1-D
Andleeb Aftab, Rohit Lall, Savita Bisen, Arun Anandanarayanan, Ajayta Rialch, Jayanta K. Chamuah, Shobha Yadav, M. Silamparasan, O. K. Raina
Acta Parasitologica.2020; 65(2): 413. CrossRef
A Survey on the Adjuvant Role of Naloxone Alone or Combined with Alum in Vaccination Against Fasciolosis in BALB/c Mice
Hakim Azizi, Hadi Mirzaeei, Amin Bagheri, Ali Bazi, Ali Khamesipour, Hajar Yaghoobi, Aliyar Mirzapour, Mehrdad Khatami, Samira Elikaee
Acta Parasitologica.2019;[Epub] CrossRef
Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp ( Ctenopharyngodon idellus ): Induces immune responses and has no damage on liver and intestine function
Zeli Tang, Hengchang Sun, TingJin Chen, Zhipeng Lin, Hongye Jiang, Xinyi Zhou, Cunbin Shi, Houjun Pan, Ouqin Chang, Pengli Ren, Jinyun Yu, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
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Comparative analysis of immune effects in mice model: Clonorchis sinensis cysteine protease generated from recombinant Escherichia coli and Bacillus subtilis spores
Zhanshuai Wu, Zeli Tang, Mei Shang, Lu Zhao, Lina Zhou, Xiangzhan Kong, Zhipeng Lin, Hengchang Sun, Tingjin Chen, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu
Parasitology Research.2017; 116(7): 1811. CrossRef
The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease
Zeli Tang, Mei Shang, Tingjin Chen, Pengli Ren, Hengchang Sun, Hongling Qu, Zhipeng Lin, Lina Zhou, Jinyun Yu, Hongye Jiang, Xinyi Zhou, Xuerong Li, Yan Huang, Jin Xu, Xinbing Yu
Parasites & Vectors.2016;[Epub] CrossRef
Immunological features of LPS from Ochrobactrum intermedium on sheep experimentally infected with Fasciola hepatica
J.M. Martínez-Pérez, D. Robles-Pérez, F.A. Rojo-Vázquez, M. Martínez-Valladares
Research in Veterinary Science.2014; 97(2): 329. CrossRef
Liver fluke vaccines in ruminants: strategies, progress and future opportunities
Hayley Toet, David M. Piedrafita, Terry W. Spithill
International Journal for Parasitology.2014; 44(12): 915. CrossRef

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Brief Communication

Immunosuppressive effect of Cryptosporidium baileyi infection on vaccination against avian infectious bronchitis in chicks: Jae Ku Rhee, Hong Ji Yang, Sim Yong Yook, Hyeon Cheol Kim; Korean J Parasitol 1998;36(3):203-206.
Published online September 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.3.203

Abstract
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Two-day-old commercial chicks were inoculated orally with 2 × 10⁶ oocysts of Cryptosporidium baileyi and vaccinated with 10^3.5 EID₅₀/head of a commercially available avian infectious bronchitis (IB) live virus vaccine at 4 and 14 days following inoculation. Chicks infected with C. baileyi were shown to have an immunosuppressive effect on IB virus. It is concluded that infection with the protozoon in early life may increase their susceptibility to IB.

Citations

Citations to this article as recorded by

Inactivation effects of slightly acidic electrolyzed water on Cryptosporidium baileyi and Eimeria tenella
Yan Huang, QiuXiang Li, ShiXin Huang, Hongyou Song, Xin Gu, Zhaoguo Chen
Veterinary Parasitology.2025; 337: 110499. CrossRef
avian infectious bronchitis
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Serum metabolomics in chickens infected with Cryptosporidium baileyi
Xue-Mei Wu, Xin Yang, Xian-Cheng Fan, Xi Chen, Yu-Xin Wang, Long-Xian Zhang, Jun-Ke Song, Guang-Hui Zhao
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