Detection of IgG antibodies with immunofluorescent antibody technique in human trichomoniasis

, Yoon, Kyong; , Kim, Kyong Min; , Ahn, Myong Hee; , Min, Duk Young; , Cha, Dong Soo

doi:1987.25.1.7

Korean J Parasito > Volume 25(1):1987 > Article

Original Article


Korean J Parasitol. 1987 Jun;25(1):7-12. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1987.25.1.7
Copyright © 1987 by The Korean Society for Parasitology


Detection of IgG antibodies with immunofluorescent antibody technique in human trichomoniasis
Kyong Yoon,Kyong Min Kim,Myong Hee Ahn,Duk Young Min and Dong Soo Cha^*
Department of Parasitology, Hanyang University, Medical College, Korea.
^*Department of Obstetrics and Gynecology, Won-Ju Medical College Yonsei University, Korea.


Abstract
The indirect fluorescent antibody(IFA) test was used to detect serum IgG and IgM antibodies to Trichomonas vaginalis in 31 vaginal trichomoniasis, 7 candidiasis and in 20 non-infected healthy wonem with antigen prepared from axenic culture of Trichomonas vaginalis isolated from vulvovaginitis patient. The results were as follows: In 31 vaginal trichomoniasis the positive reactions of IgG antibody were 27 in the 1/8 dilution or higher and 4 in the 1/4 dilution whereas in healthy women the reaction showed signigicantly low as in the 1/4 dilution of below. The sensitivity and specificity of IFA test for IgG antibody to trichomonad antigen in this study were 87.1% and 100%, respectively. No significant difference of IgM antibody levels between vaginal trichomoniasis and healthy women was observed. No relation between the levels of IgG and IgM antibodies to trichomonad antigen by IFA test was observed. No relation between the time lapse and the level of serum IgG antibodies in IFA test of vaginal trichomoniasis was regarded. In conclusion the present study suggests that IFA test in trichomoniasis could be a useful tool for detection of anti-trichomonad IgG antibodies and applicable as an immundiagnostic method.

Figures


	Fig. 1 Distribution of serum IgG antibody titers by indirect fluorescent antibody test in vaginal trichomoniasis, candidiasis and healthy controls.


	Fig. 2 Distribution of serum IgM antibody titers by indirect fluorescent antibody test in vaginal trichomoniasis and healthy controls.

Tables


	Table 1 Serum IgG antibody titers against trichomonad antigen by indirect fluorescent antibody test in vaginal trichomoniasis, candidiasis and healthy controls


	Table 2 Serum IgM antibody titers against trichomonad antigen by indirect fluorescent antibody test in vaginal trichomoniasis, candidiasis and healthy controls


	Table 3 Relationship between serum IgG and IgM antibody titers against trichomonad antigen by indirect fluorescent antibody test in each vaginal trichomoniasis and healthy controls


	Table 4 Distribution of serum IgG antibody titers by indirect fluorescent antibody test according to the duration of symptom in vaginal trichomoniasis

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