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Ultrastructure of the integument of adult Paragonimus westermani
Won Young Choi and Jae Eul Yoo
Department of Parasitology, Catholic Medical College, Seoul, Korea.
Abstract
The present study was performed to observe the ultrastructure of the integument of adult Paragonimus westermani. Dogs experimentally infected with 60 metacercariae of P. westermani were autopsied 4 months after the infection. Adult P. westermani were extracted from the dogs and the fine structure was studied by means of scanning and transmission electron microscope. The findings are as follows: Scanning electron microscopic findings showed that the spines and the papillae are distributed at whole body surface but the well developed spines or papaillae are shown around the oral suckrer and ventral sucker. At the end of the body, excretory pore was found, the shape was irregular. Transmission electron microscopic findings showed that plasma membrane, tegument, basal lamina, connective tissue, circular muscle layer, longitudinal muscle layer, nerve axon and tegumental cell were observed. In higher magnification, plasma membrane and bar-shaped granules were found at the outer surface of the tegument.
Figures
Figs. 1-6 I. Scanning electron microscopie findings
Fig. 1. Ventral view of the worm, showing the oral sucker (VS) (×60).
Fig. 2. Dorsal view of the worm, showing the oral sucker (OS) (×60).
Fig. 3. Fine spines and papillae (P) are noted around the oral sucker (×180).
Fig. 4. Small and fine spines and papillae are noted around the ventral sucker (×160).
Fig. 5. Small branched spines and aggregated papillae are noted in inner side of the oral sucker (×2,000).
Fig. 6. Several papillae are noted around the oral sucker (×4,200).
Figs. 7-12 I. Scanning electron microscopie findings
Fig. 7. Well developed spines and papillae are noted below the oral sucker (×1,540).
Fig. 8. Several papillae are aggregated around the oral sucker (×330).
Fig. 9. & Fig. 10. Poorly developed papillae are aggregated around the ventral sucker (Fig. 9: ×2,000, Fig. 10: ×600).
Fig. 11. Well developed large papillae (P) are noted around the excretory pore (×1,200).
Fig. 12. Excretory pore (EP) is noted at lower part of the worm (×900).
Figs. 13-16 II. Transmission electron microscopic findings
Fig. 13. Showing the cross-sectioned spine (SP), muscle (M) and tegument nucleus (N) in tegumental syncytium in ventral side of worm between oral sucker and ventral sucker (×12,000).
Fig. 14. Well developed mitochondria (M), endoplasmic reticulum (ER), rod-shaped granule (G) and fine vacuole are noted in tegumental syncytium (×10,000).
Fig. 15. Higher magnification of mitochondria (M) (×27,000).
Fig. 16. Well developed muscle (M) and thin tegumental layer are noted in dorsal side of the worm (×17,600).
Figs. 17-21 II. Transmission electron microscopic findings
Fig. 17. Nerve body (NB), sectioned spine (SP), basal lamina (BL) and connective tissue (CT) are noted in tegumental syncytium near the oral sucker (×8,000).
Fig. 18. Higher magnification of nerve body (NB) (×20,000).
Fig. 19. Plasma membrane (PM) is covering the outer tegument and bar-shaped granules are noted below the layer (×54,000).
Fig. 20. Structures below the tegument; muscle (M), connective tissue (CT), basal lamina (BL) and plasma membrane (PM) are noted (×54,000).
Fig. 21. Tegumental communication is noted from outer tegumental layer to inner tegumental cell (×54,000).
Figs. 22-25 II. Transmission electron microscopic findings
Fig. 22. Inner side of the tegumental syncytium (×6,500).
Fig. 23. Higher magnification of Fig. 22, showing the plasma membrane (PM), basal lamina (BL), connective tissue (CT), circular muscle (M) and dense body (D) (×17,500).
Fig. 24. & Fig. 25. Higher magnification of mitochondria (Mt). They have double membranes (×7,000 & ×26,000).
Figs. 26-27 II. Transmission electron microscopic findings
Fig. 26. Sectioned tegumental syncytium (Tg), spine (SP), basal lamina (BL), and muscle (M) are noted. (×2,000).
Fig. 27. Higher magnification of Fig. 26., basal lamina (BL) and connective tissue are noted between the spine (SP) and muscle layer (M) (×36,000).
Abbreviation of figures
OS
oral sucker
VS
ventral sucker
P
papillae
EP
excretory pore
SP
spine
Tg
tegument
ER
endoplasmic reticulum
V
vacuole
M
muscle
G
granule
A
nerve axon
BL
basal lamina
CT
connective tissue
Mt
mitochondria
D
dense body
N
tegumental nucleus
References
1.
Fujino T, et al. J Parasit 1979;9:435–448.
2.
Fujino T, Ishii Y, Cho DW. Surface ultrastructure of the tegument of Clonorchis sinensis newly excysted juveniles and adult worms. J Parasitol 1979;65(4):579–590.
3.
Fujino T, Ishii Y, Choi DW. The ultrastructural characterization of the tegument of Clonorchis sinensis (Cobbold, 1875) cercaria. Z Parasitenkd 1979;60(1):65–76.
4.
Fukuda K, Fujino T, Hamajima F. Crystalline inclusions in the subtegumental cells of the adult lung fluke, Paragonimus westermani. Z Parasitenkd 1982;68(2):235–238.
5.
Higo H, et al. Jpn J Parasitol 1984;33:421–427.
6.
Jeong KH, Rim HJ, Kim CW. [A Study On The Fine Structure Of Clonorchis Sinensis, A Liver Fluke: 1. The Body Wall And The Nervous System]. Korean J Parasitol 1978;16(2):156–164.
7.
Kim CH. [Ultrastructure of the integument of adult Clonorchis sinensis]. Korean J Parasitol 1968;6(3):111–122.
8.
Burton PR. Fine structure of the reproductive system of a frog lung fluke. 3. The spermatozoon and its differentiation. J Parasitol 1972;58(1):68–83.
9.
Lumsden RD, Sogandares-Bernal F. Ultrastructural manifestations of pulmonary paragonimiasis. J Parasitol 1970;56(6):1095–1109.
10.
Sato M, Oh M, Sakoda K. Electron microscopic study of spermatogenesis in the lung fluke (Paragonimus miyazakii). Z Zellforsch Mikrosk Anat 1967;77(2):232–243.
11.
Senft AW, Philpott DE, Pelofsky AH. Electron microscope observations of the integument, flame cells, and gut of Schistosoma mansoni. J Parasitol 1961;47:217–229.
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