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A histopathological study on the intestine of mice and rats experimentally infected by Fibricola Seoulensis
Soon Hyung Lee,Byong Hoon Yoo,Sung Tae Hong,Jong Yil Chai,Byong Seol Seo and Je Geun Chi*
Department of Parasitology and Institute of Endemic Deseases, College of Medicine, Seoul National University, Seoul 110, Korea.
Department of Pathology*, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract
A histopathological study was carried out on the duodenum of mice and rats experimentally infected by F. seoulensis. Each mouse was infected wit 500 metacercariae and killed after 1, 2, 3 days, 1 and 2 weeks from infection. Each rat was given 1,000 metacercariae and was examined after 1, 2, and 4 weeks from infection. The duodenal tissue sections of mice and rats were stained with hematoxylin and eosin, and PAS stained for the rats of 1 week group. The pathological findings are summarized as below. There were no differences in mucosal findings between the mice and the rats, adn between the location of duodenum, 1 and 5 cm distal to the pylorus. Each worm embraced a villus exclusively with its foliate forebody which was inserted into the intervillous spaces. The fluke pinched villous epithelia using its oral and ventral suckers. The tribocytic organ destroyed the villous epithelia deeply up to the stroma after 3 days from infection. Apparent villous changes were observed in the mice after 3 days from infection. Villous changes were shortening, widening, blunting or fusion. The villous stroma showed edema, microscopic hemorrhage, capillary congestion, dilatation of lymphatics and inflammatory cell infiltration. The cells were lymphocytes, plasma cells, eosinophils and giant cells. Rarely submucosal and transmural inflammation was encountered.
Figures
Figs. 1-6 Fig. 1. Mouse duodenum of control group, showing normal long finger-like villi, H-E stain, ×72.
Fig. 2. Duodenal villi of a mouse ond day after infection, showing 2 young worms (arrows), H-E stain, ×100.
Fig. 3. Duodenal villi of a mouse ond day after infection, showing epithelial pinching by oral sucker and flattening of epithelium (arrow), H-E stain, ×200.
Fig. 4. One day group mouse with epithelial pinching by oral sucker (arrow), H-E stain, ×200.
Fig. 5. Duodenal villi of 3 day group mouse showing adhesion of a worm to a villus (arrow), H-E stain, ×100.
Fig. 6. A cross sectioned worm (arrow) entrapping a villus with ventral curvature of forebody, H-E stain, ×140.
Figs. 7-12 Fig. 7. Coronal section of a 3 day worm (arrow), H-E stain, ×100.
Fig. 8. Sagittal section of a 3 day old worm (arrow) with epithelial destruction by tribocytic organ, H-E stain, ×100.
Fig. 9. Duodenal villi of a 3 day group mouse, widening and edema of villi and lymphatics dilatation extending to submucosa, H-E stain, ×100.
Fig. 10. A bulbous villus, a mouse of 3 day group, H-E stain, ×100.
Fig. 11. Duodenal villi of an 1 week group mouse, shortening of height, widening and fusion of villi, H-E stain, ×56.
Fig. 12. High power of a short and wide villus in Fig. 11, H-E stain, ×100.
Figs. 13-17 Fig. 13. A worm of 2 week group destroying epithelium and invading stroma with tribocytic organ, H-E stain, ×100.
Fig. 14. Duodenal villi with degenerative changes of a 2 week group mouse, H-E stain, ×40.
Fig. 15. Duodenum of a control group rat with long slender villi, H-E stain, ×56.
Fig. 16. A sagittaly sectioned worm, 1 week group rat, attached to a villus, H-E stain, ×100.
Fig. 17. Invasion of villous stroma by tribocytic organ and entrapping of a villus with foliate forebody, 1 week group rat, H-E stain, ×100.
Figs. 18-23 Fig. 18. Duodenum of an 1 week group rat, shortening, widening and fusion of villi, H-E stain, ×40.
Fig. 19. An edematous and fused villus with inflammatory cell infiltration of a rat in 1 week group, H-E stain, ×100.
Fig. 20. Submucosal inflammation, 1 week group rat, H-E stain, ×200.
Fig. 21. Transmural infiltration of inflammatory cells of a 1 week group rat, H-E stain, ×100.
Fig. 22. High power view of transmural inflammation with eosinophils, lymphocytes and plasma cells, H-E stain, ×200.
Fig. 23. Epithelial erosion by tribocytic organ of a 2 week old worm with abrupt ending capillaries, H-E stain, ×100.
Figs. 24-28 Fig. 24. Villous fusion, shortening and inflammatory cell infiltration, 2 week group rat, H-E stain, ×40.
Fig. 25. Submucosal inflammation beneath worm attachment, 2 week group rat, H-E stain, ×100.
Fig. 26. Transmural inflammation extending via blood vessels, 2 week group rat, H-E stain, ×40.
Fig. 27. Villous changes, 4 week group rat, H-E stain, ×40.
Fig. 28. High power view of a fused villus of a 4 week group rat, H-E stain, ×100.
Figs. 29-32 Fig. 29. Epithelial destruction by tribocytic organ of a 2 week old worm and an abruptly ending capillary, H-E stain, ×100.
Fig. 30. Free floating red blood cells (arrows) in an entrapped villus of a 4 week group rat, H-E stain, ×400.
Fig. 31. Duodenal villi showing decreased number of goblet cells in epithelium around worms, 1 week group rat, PAS stain, ×48.
Fig. 32. A strong PAS positive worm, especially tegument, oral and ventral suckers and tribocytic organ from a rat of 1 week group, PAS stain, ×100.
Tables
Table 1 The numbers of mice and rats experimentally infected by F. seoulensis metacercariae
Table 2 Histopathological findings of mouse duodenum in fibricoliasis
Table 3 Histopathological findings of rat duodenum in fibricoliasis
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