Thin layer immunoassay (TIA) for circulating antibody detection in clonorchiasis

Original Article


Korean J Parasitol. 1983 Jun;21(1):1-5. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1983.21.1.1
Copyright © 1983 by The Korean Society for Parasitology


Thin layer immunoassay (TIA) for circulating antibody detection in clonorchiasis
Kyung-Il Im
Department of Parasitology, College of Medicine, Hanyang University, Seoul, Korea.


Abstract
Thin layer immunoassay was carried out to demonstrate antibodies against Clonorchis sinensis in sera from clonorchiasis patients. Saline extract of adult worm was used as antigen. TIA technique was performed as described earlier by Elwing et al. (1976), but agarose was used instead of agar. The antibody titres of sera in 60 clonorchiasis cases were higher than that of 10 healthy and 10 amoebiasis cases, but not different comparing with that of 10 paragonimiasis cases. Antibody titres in clonorchiasis gave no differences according to the age, sex, EPG in feces, eosinophilia degree of blood, level of alkaline phosphatase and transaminase (SGOT, SGPT) in sera. It is suggested that, after evaluation, the TIA might supplement or be used as an alternative to other immunodiagnostic tests already in use for the diagnosis of clonorchiasis.

Figures


	Fig. 1 Antibody titres distribution by TIA in 60 clonorchiasis, 10 healthy, 10 paragonimiasis and 10 amoebiasis cases.


	Fig. 2 Antibody titres distribution by TIA in clonorchiasis cases by eggs per gram of feces (EPG).


	Fig. 3 Antibody titres in clonorchiasis by percentage of eosinophilic leukocyte.


	Fig. 4 Antibody titres by alkaline phosphatase activity in serum of clonorchiasis cases.


	Fig. 5 Antibody tetres by TIA with reference to S.G.O.T. in clonorchiasis cases.


	Fig. 6 Antibody titres by TIA with reference to S.G.P.T. in clonorchiasis cases.

Tables


	Table 1 Comparison of antibody titres examined by Thin Layer Immunoassay (TIA) in clonorchiasis and other parasitic diseases

References


1.	Sadun EH, Walton BC, Buck AA, Lee BK. The use of purified antigens in the diagnosis of clonorchiasis sinensis by means of intradermal and complement fixation tests. J Parasitol 1959;45(2):129–134.

2.	Sawada T, Nagata Y, Takei K, Sato S. Studies on the substance responsible for the skin tests on Clonorchiasis. Jpn J Exp Med 1964;34:315–322.

3.	Sawada T, Takei K, Williams JE, Moose JW. Isolation and purification of antigen from adult Clonorchis sinensis for complement fixation and precipitin tests. Exp Parasitol 1965;17(3):340–349.

4.	Sun T, Gibson JB. Antigens of Clonorchis sinensis in experimental and human infections. Am J Trop Med Hyg 1969;18(2):241–252.