The detectability of helminth eggs from feces by cellophane thick smear technique

, Chai, Jong Yil; , Yang, Young Tae; , Lee, Soon Hyung; , Seo, Byong Seol

doi:1982.20.1.14

Korean J Parasito > Volume 20(1):1982 > Article

Original Article


Korean J Parasitol. 1982 Jun;20(1):14-20. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1982.20.1.14
Copyright © 1982 by The Korean Society for Parasitology


The detectability of helminth eggs from feces by cellophane thick smear technique
Jong-Yil Chai,Young-Tae Yang,Soon-Hyung Lee and Byong-Seol Seo
Basic Medical Research Section, Seoul District Armed Forces General Hospital, Korea.
Department of Parasitology, College of Medicine,Seoul National University, Korea.


Abstract
The efficacy of the cellophane thick smear(CTS) technique was reassessed for detecting helminths eggs such as Ascaris lumbricoides, Trichuris trichiura and Clonorchis sinensis, in comparsion with the formalin-ether(F-E) one, with repeated smears per case. From the fecal samples of 411 ROK Army soldiers, a total of 3,288 smears were made and examined; 4 by CTS and 4 by F-E techniques. The expected egg detectability with one, two, three or four smears by each technique was mathemativally calculated. The results were as follows: 1. When the results of all 8 smears per case were integrated, the number of cases eith Ascaris egg were 54(13.1%), Trichuris 125(30.4%) and Clonorchis 33(8.0%). Some other kinds of helminths eggs and protozoan cysts were found from a few cases each. 2. The calculated detectability of Ascaris eggs by CTS technique was fairly high(95.8~96.3%) with 1-4 smears, while that by F-E technique was not so high(73.1~88.9%). 3. In case of Trchuris eggs, the egg detectability by CTS technique with 1-4 smears was a little lower(62.2-86.4%) than that by F-E technique(73.4 - 91.2%). 4. The detectability of Clonorchis eggs with 1-4 smears by CTS technique was the lowest(44.7~60.6%) among the three helminths as well as much lower than that by F-E technique(67.4~87.9%).

Figures


	Fig. 1 The graphic view of the calculated egg detectability according to examination techniques and number of smears. ○-○: cellophane thick smear technique, ●-●: formalin-ether technique.

Tables


	Table 1 The integrated results of stool examination both by CTS and F-E techniques with 8 smears per case


	Table 2 The detailed results of examinations on the egg-producers by CTS and F-E techniques with a total of 8 smears per case


	Table 3 The calculated egg detectability according to methods and number of smears to be examined

References


1.	Brown HW, et al. J Parasit 1927;14:88–90.

2.	Chai JY, Hong ST, Lee SH, Seo BS. [Fluctuation Of The Egg Production Amounts According To Worm Burden And Length Of Ascaris Lumbricoides]. Korean J Parasitol 1981;19(1):38–44.

3.	Cho SY, Lee SH, Rim HJ, Seo BS. An Evaluation Of Cellophane Thick Smear Technique For Mass Stool Examination. Korean J Parasitol 1969;7(1):48–52.

4.	Delgado Y, Martinez-Murray R. [Irregularities in egg-laying of Ascaris lumbricoides]. Ann Parasitol Hum Comp 1970;45(2):223–226.

5.	Faust EC, et al. Am J Hyg 1927;8:1–284.

6.	Komiya Y, et al. Jpn J Parasit 1960;9:61–68.

7.	Lee SH, Seo BS, Cho SY, Kang SY. Clinical Trial Of Oxantel Pamoate(Cp-14, 445) On Trichocephalus Trichiurus Infection. Korean J Parasitol 1976;14(1):25–31.

8.	Martin LK. Randomness of particle distribution in human feces and the resulting influence on helminth egg counting. Am J Trop Med Hyg 1965;14(5):747–759.

9.	Seo BS. Seoul J Med 1981;22:327–341.

*10.*	Seo BS, Cho SY, Kang SY, Chai JY. Anthelmintic Efficacy Of Methyl-5-Benzoylbenzimidazole-2-Carbamate(Mebendazole) Against Multiple Helminthic Infections. Korean J Parasitol 1977;15(1):11–16.

*11.*	Seo BS, Cho SY, Chai JY. Egg Discharging Patterns Of Ascaris Lumbricoides In Low Worm Burden Cases. Korean J Parasitol 1979;17(2):98–104.

*12.*	Seo BS, Cho SY, Chai JY. Frequency Distribution Of Ascaris Lumbricoides In Rural Koreans With Special Reference On The Effect Of Changing Endemicity. Korean J Parasitol 1979;17(2):105–113.

*13.*	Seo BS, Lee SH, Cho SY, Chai JY, Hong ST, Han IS, Sohn JS, Cho BH, Ahn SR, Lee SK, Chung SC, Kang KS, Shim HS, Hwang IS. An Epidemiologic Study On Clonorchiasis And Metagonimiasis In Riverside Areas In Korea. Korean J Parasitol 1981;19(2):137–150.

*14.*	Sinniah B. Daily egg production of Ascaris lumbricoides: the distribution of eggs in the faeces and the variability of egg counts. Parasitology 1982;84(1):167–175.

*15.*	Wykoff DE. Studies on Clonorchis sinensis. IV. Production of eggs in experimentally infected rabbits. J Parasitol 1959;45(1):91–94.